B9DFG3

PR
Gene name
ISE2 (EMB25, PDE317, At1g70070, F20P5.20)
Protein name
DExH-box ATP-dependent RNA helicase DExH15 chloroplastic
Names
ATP-dependent RNA helicase ISE2, Protein EMBRYO DEFECTIVE 25, Protein INCREASED SIZE EXCLUSION LIMIT 2, Protein PIGMENT DEFECTIVE 317
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT1G70070
EC number
3.6.4.13: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

6-119 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for B9DFG3

Entry ID Method Resolution Chain Position Source
AF-B9DFG3-F1 Predicted AlphaFoldDB

83 variants for B9DFG3

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH05116450 7 V>A No 1000Genomes
ENSVATH05116449 10 T>A No 1000Genomes
ENSVATH05116448 31 P>A No 1000Genomes
ENSVATH05116448 31 P>S No 1000Genomes
tmp_1_26394036_G_T 38 S>Y No 1000Genomes
ENSVATH05116446 40 I>F No 1000Genomes
ENSVATH05116445 45 H>N No 1000Genomes
ENSVATH05116444 45 H>Q No 1000Genomes
tmp_1_26394006_A_T 48 F>Y No 1000Genomes
ENSVATH05116443 51 T>S No 1000Genomes
ENSVATH05116442 57 P>L No 1000Genomes
tmp_1_26393980_G_A 57 P>S No 1000Genomes
ENSVATH01513477 69 E>D No 1000Genomes
ENSVATH01513476 70 E>D No 1000Genomes
ENSVATH13781992 87 D>E No 1000Genomes
tmp_1_26393863_T_A 96 S>C No 1000Genomes
ENSVATH01513475 96 S>N No 1000Genomes
ENSVATH01513475 96 S>T No 1000Genomes
ENSVATH01513473 110 D>N No 1000Genomes
ENSVATH05116441 111 L>F No 1000Genomes
ENSVATH00135033 116 A>T No 1000Genomes
tmp_1_26393775_T_C 125 Q>R No 1000Genomes
tmp_1_26393719_T_C 144 I>V No 1000Genomes
ENSVATH05116440 148 I>V No 1000Genomes
tmp_1_26393693_A_T 152 D>E No 1000Genomes
tmp_1_26393593_C_A 186 A>S No 1000Genomes
tmp_1_26393574_G_A 192 S>F No 1000Genomes
tmp_1_26393382_C_T 256 S>N No 1000Genomes
ENSVATH00135026 364 S>T No 1000Genomes
tmp_1_26392534_A_G 373 S>P No 1000Genomes
ENSVATH14460328 382 D>E No 1000Genomes
ENSVATH05116423 386 K>E No 1000Genomes
ENSVATH09361311 402 V>M No 1000Genomes
tmp_1_26392428_G_A 408 P>L No 1000Genomes
tmp_1_26392253_C_T 427 D>N No 1000Genomes
tmp_1_26392229_T_C 435 K>E No 1000Genomes
ENSVATH05116419 441 I>V No 1000Genomes
tmp_1_26392174_G_A 453 A>V No 1000Genomes
ENSVATH14460325 459 N>I No 1000Genomes
ENSVATH05116416 545 S>A No 1000Genomes
ENSVATH05116414 606 V>L No 1000Genomes
ENSVATH14460323 672 A>G No 1000Genomes
ENSVATH05116413 679 E>K No 1000Genomes
tmp_1_26391494_T_G 680 I>L No 1000Genomes
tmp_1_26391467_C_T 689 A>T No 1000Genomes
tmp_1_26391443_G_A,T 697 L>F No 1000Genomes
tmp_1_26391443_G_A,T 697 L>I No 1000Genomes
ENSVATH05116412 700 A>V No 1000Genomes
ENSVATH05116408 730 E>G No 1000Genomes
ENSVATH05116408 730 E>V No 1000Genomes
ENSVATH05116407 732 F>L No 1000Genomes
ENSVATH00135017 733 L>S No 1000Genomes
tmp_1_26391325_T_C 736 K>R No 1000Genomes
tmp_1_26391298_C_T 745 G>E No 1000Genomes
ENSVATH05116405 754 F>L No 1000Genomes
ENSVATH13781962 778 S>F No 1000Genomes
ENSVATH13781961 784 M>V No 1000Genomes
tmp_1_26391158_A_T 792 L>I No 1000Genomes
tmp_1_26391147_G_C 795 I>M No 1000Genomes
tmp_1_26391122_G_A 804 P>S No 1000Genomes
tmp_1_26391118_C_T 805 G>E No 1000Genomes
tmp_1_26391028_T_A 835 Y>F No 1000Genomes
ENSVATH14460321 845 A>V No 1000Genomes
tmp_1_26390941_A_G 864 M>T No 1000Genomes
tmp_1_26390919_T_A 871 E>D No 1000Genomes
tmp_1_26390918_A_T 872 S>T No 1000Genomes
tmp_1_26390866_C_G 889 W>S No 1000Genomes
tmp_1_26390819_C_A 905 V>L No 1000Genomes
ENSVATH14460320 910 E>K No 1000Genomes
tmp_1_26390786_C_A 916 A>S No 1000Genomes
ENSVATH05116399 980 D>E No 1000Genomes
ENSVATH14460319 983 R>C No 1000Genomes
ENSVATH05116398 984 I>L No 1000Genomes
ENSVATH13781958 1027 A>S No 1000Genomes
tmp_1_26390414_A_T 1040 C>S No 1000Genomes
ENSVATH13781957 1046 E>K No 1000Genomes
tmp_1_26390359_T_A 1058 Y>F No 1000Genomes
ENSVATH00135014 1064 D>G No 1000Genomes
tmp_1_26390324_C_T 1070 V>I No 1000Genomes
tmp_1_26390273_G_A 1087 H>Y No 1000Genomes
tmp_1_26390183_T_G 1117 M>L No 1000Genomes
tmp_1_26390078_G_A 1152 R>C No 1000Genomes
ENSVATH13781956 1153 S>R No 1000Genomes

No associated diseases with B9DFG3

4 regional properties for B9DFG3

Type Name Position InterPro Accession
domain Helicase, C-terminal 424 - 620 IPR001650
domain DEAD/DEAH box helicase domain 158 - 310 IPR011545
domain ATP-dependent RNA helicase Ski2/MTR4, C-terminal 996 - 1170 IPR012961
domain Helicase superfamily 1/2, ATP-binding domain 151 - 341 IPR014001

Functions

Description
EC Number 3.6.4.13 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Plastid, chloroplast
  • Cytoplasmic granule
  • Localizes to granule-like structures, probably stress granules (SGs), which number increases upon stress
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
chloroplast A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.
chloroplast stroma The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.
cytoplasmic stress granule A dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
mRNA binding Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns.
RNA binding Binding to an RNA molecule or a portion thereof.
RNA helicase activity Unwinding of an RNA helix, driven by ATP hydrolysis.

8 GO annotations of biological process

Name Definition
chloroplast rRNA processing Any rRNA processing that takes place in chloroplast.
cytidine to uridine editing The conversion of a cytosine residue to uridine in an RNA molecule by deamination.
embryo development ending in seed dormancy The process whose specific outcome is the progression of the embryo over time, from zygote formation to the end of seed dormancy. An example of this process is found in Arabidopsis thaliana.
gene silencing by RNA A process in which an RNA molecule reduces expression of target genes. This can occur pre-transcriptionally by assembly of heterochromatin and prevention of transcription or co- or post-transcriptionally by targeting RNAs for degradation or by interfering with splicing or translation. This process starts once the inhibitory RNA molecule has been transcribed, and includes processing of the RNA such as cleavage, modifications, transport from the nucleus to the cytoplasm, loading onto the RISC complex, and the effect on transcription or translation.
Group II intron splicing The splicing of Group II introns. This occurs by a ribozymic mechanism where the intron sequence forms a distinct 3D structure, characteristic of Group II introns and containing splice site consensus sequences, that is involved in catalyzing the splicing reactions, though protein factors are also required in vivo. Splicing occurs by a series of two transesterification reactions (mechanistically similar to those for splicing of nuclear mRNAs) initiated by a bulged adenosine residue within the intron sequence as the initiating nucleophile. The intron is excised as a lariat.
mRNA processing Any process involved in the conversion of a primary mRNA transcript into one or more mature mRNA(s) prior to translation into polypeptide.
plasmodesmata-mediated intercellular transport The movement of substances between cells via plasmodesmata. Plasmodesmata is a fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.
post-transcriptional gene silencing The inactivation of gene expression that occurs after thanscription.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P51979 HFM1 ATP-dependent DNA helicase MER3 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q15477 SKIV2L Helicase SKI2W Homo sapiens (Human) PR
A2PYH4 HFM1 Probable ATP-dependent DNA helicase HFM1 Homo sapiens (Human) PR
10 20 30 40 50 60
MNTLPVVSLT ASSSFKFFHF PSLHRSLSHS PNFSFTKSLI LNPNHLSFKS TLNSLSPSQS
70 80 90 100 110 120
QLYEEEDDEE EEEEDEDDDD EAADEYDNIS DEIRNSDDDD DDEETEFSVD LPTESARERV
130 140 150 160 170 180
EFRWQRVEKL RSLVRDFGVE MIDIDELISI YDFRIDKFQR LAIEAFLRGS SVVVSAPTSS
190 200 210 220 230 240
GKTLIAEAAA VSTVAKGRRL FYTTPLKALS NQKFREFRET FGDDNVGLLT GDSAINKDAQ
250 260 270 280 290 300
IVIMTTEILR NMLYQSVGMA SSGTGLFHVD AIVLDEVHYL SDISRGTVWE EIVIYCPKEV
310 320 330 340 350 360
QLICLSATVA NPDELAGWIG EIHGKTELVT STRRPVPLTW YFSTKHSLLP LLDEKGINVN
370 380 390 400 410 420
RKLSLNYLQL SASEARFRDD DDGYRKRRSK KRGGDTSYNN LVNVTDYPLS KNEINKIRRS
430 440 450 460 470 480
QVPQISDTLW HLQGKNMLPA IWFIFNRRGC DAAVQYVENF QLLDDCEKSE VELALKKFRV
490 500 510 520 530 540
LYPDAVRESA EKGLLRGIAA HHAGCLPLWK SFIEELFQRG LVKVVFATET LAAGINMPAR
550 560 570 580 590 600
TAVISSLSKK AGNERIELGP NELYQMAGRA GRRGIDEKGY TVLVQTAFEG AEECCKLVFA
610 620 630 640 650 660
GVKPLVSQFT ASYGMVLNLV AGSKVTRKSS GTEAGKVLQA GRSLEEAKKL VEKSFGNYVS
670 680 690 700 710 720
SNVTVAAKQE LAEIDNKIEI LSSEISDEAI DKKSRKLLSA RDYKEITVLK EELREEKRKR
730 740 750 760 770 780
AEQRRRMELE RFLALKPLLK GMEEGNLPFI CLEFKDSEGR EQSVPAVYLG HIDSFQGSKL
790 800 810 820 830 840
QKMMSLDESF ALNLIEDELA ADEPGKPNVK PSYYVALGSD NSWYLFTEKW VRTVYRTGFP
850 860 870 880 890 900
NIALALGDAL PREIMKNLLD KADMQWDKLA ESELGSLWRL EGSLETWSWS LNVPVLSSLS
910 920 930 940 950 960
DEDEVLHMSE EYDNAAQKYK EQRSKISRLK KKMSRSEGFR EYKKILENAN LTVEKMKRLK
970 980 990 1000 1010 1020
ARSRRLINRL EQIEPSGWKD FMRISNVIHE SRALDINTHL IFPLGETAAA IRGENELWLA
1030 1040 1050 1060 1070 1080
MVLRNKALVD LKPPQLAGVC ASLVSEGIKV RPWRDNNYIY EPSDTVVDMV NFLEDQRSSL
1090 1100 1110 1120 1130 1140
IKLQEKHEVM IPCCLDVQFS GMVEAWASGL SWKEMMMECA MDEGDLARLL RRTIDLLAQI
1150 1160 1170
PKLPDIDPVL QRSAAAAADI MDRPPISELA G