AiPD: Autoinhibited Protein Database

Gene name	At4g08350 (T28D5.40)
Protein name	Putative transcription elongation factor SPT5 homolog 1
Names
Species	Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway	ath:AT4G08350
EC number
Protein Class

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

745-946 (Predicted disordered autoinhibitory region) PR

745-946 (Predicted disordered autoinhibitory region)

cis-regPred

No accessory elements

Entry ID	Method	Resolution	Chain	Position	Source
AF-Q9STN3-F1	Predicted				AlphaFoldDB

Variant ID(s)	Position	Change	Diseaes Association	Provenance
ENSVATH06566926	8	D>G	No	1000Genomes
tmp_4_5286407_T_A	19	D>E	No	1000Genomes
tmp_4_5286536_A_C	62	E>D	No	1000Genomes
tmp_4_5286675_G_C	109	A>P	No	1000Genomes
ENSVATH00478548	123	D>G	No	1000Genomes
ENSVATH14081668	127	D>N	No	1000Genomes
ENSVATH02741322	180	R>C	No	1000Genomes
tmp_4_5287269_G_A	219	A>T	No	1000Genomes
tmp_4_5287694_G_C	295	V>L	No	1000Genomes
tmp_4_5288455_G_A	449	V>I	No	1000Genomes
ENSVATH14081671	490	T>A	No	1000Genomes
tmp_4_5288682_A_C	492	E>D	No	1000Genomes
ENSVATH14081672	494	A>E	No	1000Genomes
ENSVATH06566948	595	Q>E	No	1000Genomes
tmp_4_5289287_C_T	597	R>C	No	1000Genomes
ENSVATH06566949	616	K>R	No	1000Genomes
ENSVATH06566951	637	H>Q	No	1000Genomes
ENSVATH14081705	674	N>T	No	1000Genomes
ENSVATH11265707	678	P>S	No	1000Genomes
tmp_4_5289702_G_A	679	A>T	No	1000Genomes
ENSVATH06566976	853	E>D	No	1000Genomes
ENSVATH14081725	855	P>L	No	1000Genomes
ENSVATH06566977	872	A>V	No	1000Genomes
ENSVATH06566978	903	A>V	No	1000Genomes
ENSVATH06566980	924	M>I	No	1000Genomes
tmp_4_5291629_C_T	960	S>L	No	1000Genomes
tmp_4_5291859_C_T,G	971	S>R	No	1000Genomes
ENSVATH00478578	971	S>T	No	1000Genomes
ENSVATH14081731	982	S>N	No	1000Genomes
tmp_4_5292068_C_G	1041	P>R	No	1000Genomes

Type	Name	Position	InterPro Accession
domain	NGN domain	183 - 265	IPR005100
domain	KOW	273 - 300	IPR005824-1
domain	KOW	425 - 452	IPR005824-2
domain	KOW	477 - 522	IPR005824-3
domain	KOW	601 - 628	IPR005824-4
domain	KOW	712 - 739	IPR005824-5
domain	KOW	988 - 1015	IPR005824-6
conserved_site	Ribosomal protein L24/L26, conserved site	429 - 446	IPR005825
domain	NusG-like, N-terminal	181 - 268	IPR006645
domain	Spt5 transcription elongation factor, N-terminal	90 - 177	IPR022581
domain	NGN domain, eukaryotic	183 - 266	IPR039385
domain	Spt5, KOW domain repeat 1	277 - 314	IPR041973
domain	Spt5, KOW domain repeat 2	426 - 476	IPR041975
domain	Spt5, KOW domain repeat 3	477 - 527	IPR041976
domain	Spt5, KOW domain repeat 4	605 - 647	IPR041977
domain	Spt5, KOW domain repeat 5	711 - 758	IPR041978
domain	Spt5, KOW domain repeat 6	982 - 1039	IPR041980

		Description
EC Number
Subcellular Localization	Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

Name	Definition
DSIF complex	A heterodimeric protein complex formed of Spt4 and Spt5 proteins which is expressed in eukaryotes from yeast to man. DSIF is an inhibitory elongation factor that promotes RNA polymerase II transcriptional pausing, but can also stimulate transcriptional elongation under certain conditions, and may play a role in RNA processing via its physical association with mRNA capping enzymes.
ribosome	An intracellular organelle, about 200 A in diameter, consisting of RNA and protein. It is the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). It consists of two subunits, one large and one small, each containing only protein and RNA. Both the ribosome and its subunits are characterized by their sedimentation coefficients, expressed in Svedberg units (symbol: S). Hence, the prokaryotic ribosome (70S) comprises a large (50S) subunit and a small (30S) subunit, while the eukaryotic ribosome (80S) comprises a large (60S) subunit and a small (40S) subunit. Two sites on the ribosomal large subunit are involved in translation, namely the aminoacyl site (A site) and peptidyl site (P site). Ribosomes from prokaryotes, eukaryotes, mitochondria, and chloroplasts have characteristically distinct ribosomal proteins.

Name	Definition
mRNA binding	Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns.
structural constituent of ribosome	The action of a molecule that contributes to the structural integrity of the ribosome.

Name	Definition
regulation of DNA-templated transcription elongation	Any process that modulates the frequency, rate or extent of transcription elongation, the extension of an RNA molecule after transcription initiation and promoter clearance by the addition of ribonucleotides catalyzed by a DNA-dependent RNA polymerase.
regulation of transcription by RNA polymerase II	Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
transcription elongation by RNA polymerase II promoter	The extension of an RNA molecule after transcription initiation and promoter clearance at an RNA polymerase II promoter by the addition of ribonucleotides catalyzed by RNA polymerase II.
translation	The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA or circRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA or circRNA. Translation ends with the release of a polypeptide chain from the ribosome.

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q9V460	Spt5	Transcription elongation factor SPT5	Drosophila melanogaster (Fruit fly)	PR
O00267	SUPT5H	Transcription elongation factor SPT5	Homo sapiens (Human)	PR
O55201	Supt5h	Transcription elongation factor SPT5	Mus musculus (Mouse)	PR