AiPD: Autoinhibited Protein Database

Q96FW1

Gene name	OTUB1 (OTB1, OTU1, HSPC263)
Protein name	Ubiquitin thioesterase OTUB1
Names	Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, hOTU1, Ubiquitin-specific-processing protease OTUB1
Species	Homo sapiens (Human)
KEGG Pathway	hsa:55611
EC number	3.4.19.12: Omega peptidases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-51 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

1-51 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

8 structures for Q96FW1

Entry ID	Method	Resolution	Chain	Position	Source
2ZFY	X-ray	169 A	A	40-271	PDB
3VON	X-ray	315 A	A/H/O/V/c/j	45-271	PDB
4DDG	X-ray	330 A	A/B/C/J/K/L	25-271	PDB
4DDI	X-ray	380 A	A/B/C	25-271	PDB
4DHZ	X-ray	311 A	A	1-45	PDB
4I6L	X-ray	249 A	A	45-271	PDB
4LDT	X-ray	190 A	A	1-45	PDB
AF-Q96FW1-F1	Predicted				AlphaFoldDB

99 variants for Q96FW1

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs1169307056	6	P>S	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	NCI-TCGA
rs1169307056	6	P>T	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	NCI-TCGA
rs146537845	26	Y>C	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA381057914 rs1565184199	33	Q>*		No	ClinGen Ensembl
CA6066817 rs748757526	34	Q>R		No	ClinGen ExAC gnomAD
CA381057957 rs1565184210	36	R>*		No	ClinGen Ensembl
TCGA novel	36	R>Q	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1028085841 CA223770875	38	Q>H		No	ClinGen Ensembl
TCGA novel	42	A>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA6066842 rs760399947	44	Q>K		No	ClinGen ExAC gnomAD
TCGA novel	46	P>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs183970848 CA223771158	50	E>V		No	ClinGen 1000Genomes
rs369002057 CA6066843	51	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
rs1215504265 CA381058667	51	R>W		No	ClinGen gnomAD
rs759850958 CA6066845	53	E>G		No	ClinGen ExAC gnomAD
rs1419036261 CA381058699	54	L>R		No	ClinGen gnomAD
CA6066846 rs765392727	54	L>V		No	ClinGen ExAC gnomAD
rs1488269742 CA381058707	55	S>L		No	ClinGen TOPMed
rs758905157 CA6066848	56	V>A		No	ClinGen ExAC gnomAD
CA381058733 rs1289003922	58	Y>C		No	ClinGen gnomAD
CA381058748 rs1234905006	59	K>N		No	ClinGen TOPMed
rs17850645 CA223771191	61	Y>C		No	ClinGen Ensembl
rs1590777982 CA381058767	61	Y>D		No	ClinGen Ensembl
rs904675514 CA223771195	63	E>K		No	ClinGen TOPMed
rs781653508 CA6066852	67	I>V		No	ClinGen ExAC gnomAD
CA6066853 rs748872229	68	Y>C		No	ClinGen ExAC TOPMed gnomAD
CA381058898 rs1335258020	72	I>N		No	ClinGen gnomAD
rs1590782188 CA381059628	74	D>A		No	ClinGen Ensembl
CA381059634 rs1590782191	74	D>E		No	ClinGen Ensembl
rs368884174 CA223776134	76	H>Q		No	ClinGen ESP TOPMed
rs1470682602 CA381059657	77	K>E		No	ClinGen gnomAD
rs1020389687 CA223776135	77	K>I		No	ClinGen Ensembl
rs1565186477 CA381059671	78	K>T		No	ClinGen Ensembl
rs148874418 CA223776146	80	S>L		No	ClinGen ESP gnomAD
rs1565186492 CA381059769	87	P>R		No	ClinGen Ensembl
TCGA novel	88	D>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA381059827 rs1375511814	92	F>L		No	ClinGen gnomAD
COSM1509370 CA381059833 rs1436275463	93	Y>C	lung Variant assessed as Somatic; 0.0 impact. [Cosmic, NCI-TCGA]	No	ClinGen cosmic curated NCI-TCGA gnomAD
COSM193918 CA381059842 rs1254707597	94	R>Q	large_intestine Variant assessed as Somatic; impact. [Cosmic, NCI-TCGA]	No	ClinGen cosmic curated NCI-TCGA TOPMed
COSM1355600 CA381059840 rs1565186502	94	R>W	Variant assessed as Somatic; 0.0 impact. large_intestine [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated Ensembl NCI-TCGA
TCGA novel	98	F>L	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1442604675 CA381059883	100	H>Y		No	ClinGen gnomAD
rs775884042 CA6066883	101	L>M		No	ClinGen ExAC TOPMed gnomAD
CA381059889 rs775884042	101	L>V		No	ClinGen ExAC TOPMed gnomAD
TCGA novel	104	L>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1304607311 CA381059922	106	D>A		No	ClinGen gnomAD
rs1300028482 CA381059919	106	D>H		No	ClinGen Ensembl
CA381059967 rs1338431658	112	Q>E		No	ClinGen TOPMed
COSM929978 CA13550409 rs147019398	113	R>W	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ESP NCI-TCGA TOPMed gnomAD
rs751755933	156	A>T	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	NCI-TCGA
rs750475835 CA6066982	158	L>P		No	ClinGen ExAC gnomAD
rs1467186831 CA381060327	160	A>S		No	ClinGen TOPMed
TCGA novel	161	S>missing	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs756590037 CA6066983	163	N>S		No	ClinGen ExAC gnomAD
rs1438775569	169	D>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs755363848 CA6066986	174	Y>C		No	ClinGen ExAC gnomAD
TCGA novel	176	R>Q	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA381060454 rs1347627290	179	T>I		No	ClinGen TOPMed gnomAD
CA223776927 rs926342684	180	S>L		No	ClinGen TOPMed gnomAD
rs1236924379 CA381060470	182	Y>C		No	ClinGen gnomAD
rs937695868	185	R>C	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs942716031 CA223776942	185	R>H		No	ClinGen TOPMed
rs745390279	186	E>K	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	NCI-TCGA
CA6066992 rs769730926	187	S>R		No	ClinGen ExAC gnomAD
rs11537828 CA6066995 CA223776954	195	E>D		No	ClinGen ExAC gnomAD
rs1033480567 CA223776966	197	G>V		No	ClinGen Ensembl
CA6066997 rs761953822	198	R>Q	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
rs756050985	198	R>W	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	202	E>missing	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	203	F>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1030215946	216	D>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1256016375 CA381060770	222	A>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed gnomAD
CA381060790 rs1176243407	226	A>S		No	ClinGen TOPMed
CA6067027 rs755858584	227	L>P		No	ClinGen ExAC gnomAD
CA6067029 rs200423792	228	S>R		No	ClinGen ExAC gnomAD
CA381060846 rs778856937	234	E>D		No	ClinGen ExAC TOPMed gnomAD
rs1170376843 CA381060874	238	R>G		No	ClinGen gnomAD
CA223777239 rs945014300	239	G>S	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed gnomAD
CA6067035 rs148181122	240	E>D		No	ClinGen ESP ExAC TOPMed gnomAD
CA6067034 rs773118356	240	E>K		No	ClinGen ExAC gnomAD
COSM929981 rs1332837418 CA381060898	242	G>S	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated NCI-TCGA gnomAD
rs910361138 CA223777252	243	T>I		No	ClinGen gnomAD
rs910361138 CA381060906	243	T>S		No	ClinGen gnomAD
rs1363127053 CA381060912	244	T>I		No	ClinGen TOPMed gnomAD
rs765447237 CA6067039	245	N>S		No	ClinGen ExAC gnomAD
rs753233012 CA6067040	246	P>L		No	ClinGen ExAC gnomAD
rs764357623 CA6067042	247	H>Y		No	ClinGen ExAC gnomAD
CA6067044 rs755663560	248	I>V		No	ClinGen ExAC TOPMed gnomAD
rs779780540 CA6067045	252	G>A		No	ClinGen ExAC TOPMed gnomAD
CA381060999 rs779780540	252	G>D		No	ClinGen ExAC TOPMed gnomAD
CA381061001 rs779780540	252	G>V		No	ClinGen ExAC TOPMed gnomAD
rs754453978 CA6067047	254	E>K		No	ClinGen ExAC gnomAD
rs778444099 CA6067048	256	K>E		No	ClinGen ExAC gnomAD
CA381061053 rs748068381	256	K>N		No	ClinGen ExAC TOPMed gnomAD
CA381061079 rs1403633673	258	Y>C		No	ClinGen gnomAD
TCGA novel	262	R>Q	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1397830557 CA381061123	262	R>W		No	ClinGen TOPMed
CA381061159 rs1402066808	265	H>P		No	ClinGen gnomAD
CA381061229 rs1286420368 COSM1663296	270	Y>H	kidney [Cosmic]	No	ClinGen cosmic curated gnomAD

No associated diseases with Q96FW1

1 regional properties for Q96FW1

Type	Name	Position	InterPro Accession
domain	OTU domain	80 - 271	IPR003323

Functions

		Description
EC Number	3.4.19.12	Omega peptidases
Subcellular Localization	Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

4 GO annotations of cellular component

Name	Definition
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
extracellular exosome	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
nucleoplasm	That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

4 GO annotations of molecular function

Name	Definition
cysteine-type deubiquitinase activity	An thiol-dependent isopeptidase activity that cleaves ubiquitin from a target protein to which it is conjugated.
deNEDDylase activity	An isopeptidase activity that cleaves NEDD8 from a target protein to which it is conjugated.
ubiquitin binding	Binding to ubiquitin, a protein that when covalently bound to other cellular proteins marks them for proteolytic degradation.
ubiquitin protein ligase binding	Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.

6 GO annotations of biological process

Name	Definition
adaptive immune response	An immune response mediated by cells expressing specific receptors for antigen produced through a somatic diversification process, and allowing for an enhanced secondary response to subsequent exposures to the same antigen (immunological memory).
cellular response to DNA damage stimulus	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
DNA repair	The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
negative regulation of double-strand break repair	Any process that stops, prevents or reduces the frequency, rate or extent of double-strand break repair.
negative regulation of histone H2A K63-linked ubiquitination	Any process that stops, prevents or reduces the frequency, rate or extent of histone H2A K63-linked ubiquitination.
protein K48-linked deubiquitination	A protein deubiquitination process in which a K48-linked ubiquitin chain, i.e. a polymer of ubiquitin formed by linkages between lysine residues at position 48 of the ubiquitin monomers, is removed from a protein.

1 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q7TQI3	Otub1	Ubiquitin thioesterase OTUB1	Mus musculus (Mouse)	PR

10	20	30	40	50	60
MAAEEPQQQK	QEPLGSDSEG	VNCLAYDEAI	MAQQDRIQQE	IAVQNPLVSE	RLELSVLYKE
70	80	90	100	110	120
YAEDDNIYQQ	KIKDLHKKYS	YIRKTRPDGN	CFYRAFGFSH	LEALLDDSKE	LQRFKAVSAK
130	140	150	160	170	180
SKEDLVSQGF	TEFTIEDFHN	TFMDLIEQVE	KQTSVADLLA	SFNDQSTSDY	LVVYLRLLTS
190	200	210	220	230	240
GYLQRESKFF	EHFIEGGRTV	KEFCQQEVEP	MCKESDHIHI	IALAQALSVS	IQVEYMDRGE
250	260	270
GGTTNPHIFP	EGSEPKVYLL	YRPGHYDILY	K