AiPD: Autoinhibited Protein Database

Gene name	Cdkal1
Protein name	Threonylcarbamoyladenosine tRNA methylthiotransferase
Names	CDK5 regulatory subunit-associated protein 1-like 1, tRNA-t(6)A37 methylthiotransferase
Species	Mus musculus (Mouse)
KEGG Pathway	mmu:68916
EC number	2.8.4.5: Transferring alkylthio groups
Protein Class

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

479-553 (Predicted disordered autoinhibitory region) PR

479-553 (Predicted disordered autoinhibitory region)

cis-regPred

No accessory elements

Entry ID	Method	Resolution	Chain	Position	Source
AF-Q91WE6-F1	Predicted				AlphaFoldDB

Variant ID(s)	Position	Change	Diseaes Association	Provenance
rs237825682	5	S>A	No	EVA
rs3389272140	45	K>*	No	EVA
rs258966938	51	P>L	No	EVA
rs3389283662	90	G>D	No	EVA
rs3404109153	104	L>I	No	EVA
rs3403990706	108	C>*	No	EVA
rs3403307636	108	C>S	No	EVA
rs3404324397	108	C>S	No	EVA
rs3389274384	138	V>I	No	EVA
rs3389283681	140	Q>H	No	EVA
rs3389283688	185	G>V	No	EVA
rs3389248487	186	K>SDG*	No	EVA
rs3389260592	187	R>C	No	EVA
rs3389277915	188	L>M	No	EVA
rs3389248511	202	P>Q	No	EVA
rs3389283649	206	I>F	No	EVA
rs3389277144	319	I>L	No	EVA
rs3389234321	341	F>L	No	EVA
rs3389248488	343	R>G	No	EVA
rs30005788	350	E>K	No	EVA
rs3389278144	438	Q>H	No	EVA
rs3389234273	454	H>Q	No	EVA
rs3389206219	478	I>M	No	EVA
rs218288291	532	C>Y	No	EVA
rs260419135	551	Y>F	No	EVA
rs234843240	559	T>A	No	EVA
rs3389260659	560	G>D	No	EVA
rs213201235	562	A>V	No	EVA
rs3389268357	563	L>F	No	EVA
rs226458761	566	L>F	No	EVA
rs257606357	573	D>E	No	EVA
rs29850511	575	L>M	No	EVA
rs232649747	577	T>I	No	EVA

Type	Name	Position	InterPro Accession
domain	TRAM domain	430 - 492	IPR002792
domain	Elp3/MiaA/NifB-like, radical SAM core domain	202 - 421	IPR006638
domain	Radical SAM	199 - 442	IPR007197
domain	Methylthiotransferase, N-terminal	63 - 171	IPR013848
conserved_site	Methylthiotransferase, conserved site	207 - 227	IPR020612

		Description
EC Number	2.8.4.5	Transferring alkylthio groups
Subcellular Localization	Endoplasmic reticulum membrane ; Single-pass membrane protein	Is a tail-anchored protein that exploits the TCR40 assisted pathway for insertion into the endoplasmic reticulum
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

Name	Definition
endoplasmic reticulum	The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached).
endoplasmic reticulum membrane	The lipid bilayer surrounding the endoplasmic reticulum.
integral component of membrane	The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
rough endoplasmic reticulum	The rough (or granular) endoplasmic reticulum (ER) has ribosomes adhering to the outer surface; the ribosomes are the site of translation of the mRNA for those proteins which are either to be retained within the cisternae (ER-resident proteins), the proteins of the lysosomes, or the proteins destined for export from the cell. Glycoproteins undergo their initial glycosylation within the cisternae.

Name	Definition
4 iron, 4 sulfur cluster binding	Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
metal ion binding	Binding to a metal ion.
N6-threonylcarbomyladenosine methylthiotransferase activity	Catalysis of the methylthiolation (-SCH3 addition) at the C2 of the adenosine ring of N6-threonylcarbomyladenosine (t6A) in tRNA, to form 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A).
tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase	Catalysis of the reaction: N(6)-L-threonylcarbamoyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-L-threonylcarbamoyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.

Name	Definition
maintenance of translational fidelity	Suppression of the occurrence of translational errors, such as codon-anticodon mis-paring, during the process of translation of a protein using an mRNA template.
tRNA methylthiolation	The addition of a methylthioether group (-SCH3) to a nucleotide in a tRNA molecule.

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q7K4W1	CG6550	Threonylcarbamoyladenosine tRNA methylthiotransferase	Drosophila melanogaster (Fruit fly)	PR
Q5VV42	CDKAL1	Threonylcarbamoyladenosine tRNA methylthiotransferase	Homo sapiens (Human)	PR
Q6P4Y0	cdkal1	Threonylcarbamoyladenosine tRNA methylthiotransferase	Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)	PR
Q6PG34	cdkal1	Threonylcarbamoyladenosine tRNA methylthiotransferase	Danio rerio (Zebrafish) (Brachydanio rerio)	PR