Q6PG34
Gene name |
cdkal1 (zgc:65864) |
Protein name |
Threonylcarbamoyladenosine tRNA methylthiotransferase |
Names |
CDK5 regulatory subunit-associated protein 1-like 1, tRNA-t(6)A37 methylthiotransferase |
Species |
Danio rerio (Zebrafish) (Brachydanio rerio) |
KEGG Pathway |
dre:393600 |
EC number |
2.8.4.5: Transferring alkylthio groups |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q6PG34
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q6PG34-F1 | Predicted | AlphaFoldDB |
No variants for Q6PG34
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q6PG34 | |||||
No associated diseases with Q6PG34
5 regional properties for Q6PG34
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | TRAM domain | 426 - 488 | IPR002792 |
| domain | Elp3/MiaA/NifB-like, radical SAM core domain | 198 - 417 | IPR006638 |
| domain | Radical SAM | 58 - 488 | IPR007197 |
| domain | Methylthiotransferase, N-terminal | 59 - 167 | IPR013848 |
| conserved_site | Methylthiotransferase, conserved site | 203 - 223 | IPR020612 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.8.4.5 | Transferring alkylthio groups |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| endoplasmic reticulum | The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached). |
| endoplasmic reticulum membrane | The lipid bilayer surrounding the endoplasmic reticulum. |
| integral component of membrane | The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| 4 iron, 4 sulfur cluster binding | Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. |
| metal ion binding | Binding to a metal ion. |
| N6-threonylcarbomyladenosine methylthiotransferase activity | Catalysis of the methylthiolation (-SCH3 addition) at the C2 of the adenosine ring of N6-threonylcarbomyladenosine (t6A) in tRNA, to form 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A). |
| tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase | Catalysis of the reaction: N(6)-L-threonylcarbamoyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-L-threonylcarbamoyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine. |
4 GO annotations of biological process
| Name | Definition |
|---|---|
| endocrine pancreas development | The process whose specific outcome is the progression of the endocrine pancreas over time, from its formation to the mature structure. The endocrine pancreas is made up of islet cells that produce insulin, glucagon and somatostatin. |
| pancreas regeneration | The regrowth of a destroyed pancreas. |
| tRNA methylthiolation | The addition of a methylthioether group (-SCH3) to a nucleotide in a tRNA molecule. |
| type B pancreatic cell development | The process whose specific outcome is the progression of a type B pancreatic cell over time, from its formation to the mature structure. A type B pancreatic cell is a cell located towards center of the islets of Langerhans that secretes insulin. |
4 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q7K4W1 | CG6550 | Threonylcarbamoyladenosine tRNA methylthiotransferase | Drosophila melanogaster (Fruit fly) | PR |
| Q5VV42 | CDKAL1 | Threonylcarbamoyladenosine tRNA methylthiotransferase | Homo sapiens (Human) | PR |
| Q91WE6 | Cdkal1 | Threonylcarbamoyladenosine tRNA methylthiotransferase | Mus musculus (Mouse) | PR |
| Q6P4Y0 | cdkal1 | Threonylcarbamoyladenosine tRNA methylthiotransferase | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MMALVCDTAV | DDIEDMVRIT | DVTPLQRQAA | RKSVVPRARK | HKQETGEQMQ | TDSVIPGMQK |
| 70 | 80 | 90 | 100 | 110 | 120 |
| VWLKTWGCSH | NSSDGEYMAG | QLAVAGYQIT | EDSSDADLWL | LNSCTVKSPA | EDHFRNAIRK |
| 130 | 140 | 150 | 160 | 170 | 180 |
| AQEQNKKVVL | AGCVPQAQPR | MDYIKDLSII | GVQQIDRVVE | VVDEAIKGHS | VRLLGQKKEK |
| 190 | 200 | 210 | 220 | 230 | 240 |
| GKRLGGARLD | LPKIRKNPLI | EIISINTGCL | NACTYCKTKH | ARGDLASYPV | EELVERVRQS |
| 250 | 260 | 270 | 280 | 290 | 300 |
| FQEGVCEIWL | TSEDTGAYGR | DIGSDLPTLL | WRLVEEIPEG | AMLRLGMTNP | PYILEHLEEM |
| 310 | 320 | 330 | 340 | 350 | 360 |
| SRILQHPRVF | SFLHVPLQSA | SDSVLMEMRR | EYCCADFTHL | VDYLKERVPG | ITIATDIICG |
| 370 | 380 | 390 | 400 | 410 | 420 |
| FPGETDEDFE | QTLALVRRYR | FPSLFINQFY | PRPGTPAALM | QQLPAHVKKQ | RTKELSALFH |
| 430 | 440 | 450 | 460 | 470 | 480 |
| SYRPYDHKMG | EQQQVLVTEE | SFDSQYYVAH | NKFYEQVLVP | KRPEYLGKMV | QVEVYECGKH |
| 490 | 500 | 510 | 520 | 530 | 540 |
| YMKGRPLEEA | PLRTAYTTAP | LLKGQVSGHT | QAGVCEPQCW | MPDGMRILAV | VLLLSAVLLA |
| LLMEKLL |