Q8CFK2

PR
Gene name
Brf1
Protein name
Transcription factor IIIB 90 kDa subunit
Names
TFIIIB90, mTFIIIB90, B-related factor 1, BRF-1
Species
Mus musculus (Mouse)
KEGG Pathway
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

319-606 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q8CFK2

Entry ID Method Resolution Chain Position Source
AF-Q8CFK2-F1 Predicted AlphaFoldDB

No variants for Q8CFK2

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q8CFK2

No associated diseases with Q8CFK2

6 regional properties for Q8CFK2

Type Name Position InterPro Accession
domain Brf1, TBP-binding domain 449 - 546 IPR011665
domain Zinc finger, TFIIB-type 2 - 45 IPR013137
domain Transcription factor TFIIB, cyclin-like domain 83 - 172 IPR013150-1
domain Transcription factor TFIIB, cyclin-like domain 177 - 269 IPR013150-2
domain Cyclin-like domain 91 - 172 IPR013763-1
domain Cyclin-like domain 185 - 269 IPR013763-2

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
transcription factor TFIIIB complex A transcription factor complex that is involved in regulating transcription from RNA polymerase III (Pol III) promoters. TFIIIB contains the TATA-binding protein (TBP) and two Pol III-specific proteins, B'' and BRF.
transcription preinitiation complex A protein-DNA complex composed of proteins binding promoter DNA to form the transcriptional preinitiation complex (PIC), the formation of which is a prerequisite for transcription.

4 GO annotations of molecular function

Name Definition
metal ion binding Binding to a metal ion.
RNA polymerase III general transcription initiation factor activity A general transcription initiation factor activity that contributes to transcription start site selection and transcription initiation of genes transcribed by RNA polymerase III. Factors required for RNA polymerase III transcription initiation include TFIIIA, TFIIIB and TFIIIC. RNA polymerase III transcribes genes encoding short RNAs, including tRNAs, 5S rRNA, U6 snRNA, the short ncRNA component of RNases P, the mitochondrial RNA processing (MRP) RNA, the signal recognition particle SRP RNA, and in higher eukaryotes a number of micro and other small RNAs, though there is some variability across species as to whether a given small noncoding RNA is transcribed by RNA polymerase II or RNA polymerase III.
RNA polymerase III type 3 promoter sequence-specific DNA binding Binding to a sequence of DNA that is a part of a type 3 promoter that controls transcription by RNA polymerase III (Pol III). A type 3 Pol III promoter is composed of elements upstream of the transcription start site, including a TATA box. The human U6 snRNA gene has a type 3 promoter. Type 3 Pol III promoters have not been observed in S. cerevisiae.
TBP-class protein binding Binding to a member of the class of TATA-binding proteins (TBP), including any of the TBP-related factors (TRFs).

5 GO annotations of biological process

Name Definition
DNA-templated transcription initiation The initial step of transcription, consisting of the assembly of the RNA polymerase preinitiation complex (PIC) at a gene promoter, as well as the formation of the first few bonds of the RNA transcript. Transcription initiation includes abortive initiation events, which occur when the first few nucleotides are repeatedly synthesized and then released, and ends when promoter clearance takes place.
positive regulation of transcription by RNA polymerase III Any process that activates or increases the frequency, rate or extent of transcription mediated by RNA polymerase III.
regulation of mRNA stability Any process that modulates the propensity of mRNA molecules to degradation. Includes processes that both stabilize and destabilize mRNAs.
transcription by RNA polymerase III The synthesis of RNA from a DNA template by RNA polymerase III, originating at an RNAP III promoter.
transcription preinitiation complex assembly The aggregation, arrangement and bonding together of proteins on promoter DNA to form the transcriptional preinitiation complex (PIC), required for transcription.

1 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q92994 BRF1 Transcription factor IIIB 90 kDa subunit Homo sapiens (Human) PR
10 20 30 40 50 60
MTGRVCRGCG GTDIELDAAR GDAVCTGCGS VLEDNIIVSE VQFVENSGGG SSAVGQFVSL
70 80 90 100 110 120
DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGSQL QLNQHCLDTA FNFFKMAVSK
130 140 150 160 170 180
HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVNV YVLGKTFLLL ARELCINAPA
190 200 210 220 230 240
IDPCLYIPRF AHLLEFGEKN HEVSMTALRL LQRMKRDWMH TGRRPSGLCG AALLVAARMH
250 260 270 280 290 300
DFRRTVKEVI SVVKVCESTL RKRLTEFEDT PTSQLTIDEF MKIDLEEECD PPSYTAGQRK
310 320 330 340 350 360
LRMKQLEQVL SKKLEEVEGE ISSYQDAIEI ELENSRPKAK GALANLSKDG SGEDATSSPR
370 380 390 400 410 420
CEEDTEDEEL EAAASHMNKD FYRELLGDDD GSEAAGDPDG GSRPLALESL LGPLPTAASL
430 440 450 460 470 480
GISDSIRECI SSPSGDPKDS SGDGELDLSG IDDLEIDRYI LNESEARVKA ELWMRENAEY
490 500 510 520 530 540
LREQKEKEAR IAKEKELGIY KEHKPKKSCK RREPILASTA GEAIEKMLEQ KKISSKINYS
550 560 570 580 590 600
VLRDLNSKGG GSPPRDDSQP PERASTKKLS RRKRATTRNS ADPGTSTGKR LRPLLSAQPA
610 620 630 640 650 660
KKAAVGEALL LSSPALGAEP VKPSAVLVES GPVPYHPEED ADEEDAEDED GEPCVSALQM
670
MGGNDYGCDG DEDDGY