Q5ZIW1

PR
Gene name
TWNK
Protein name
Twinkle mtDNA helicase
Names
Progressive external ophthalmoplegia 1 protein homolog, Twinkle protein, mitochondrial
Species
Gallus gallus (Chicken)
KEGG Pathway
gga:425626
EC number
5.6.2.3: Enzymes altering nucleic acid conformation
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-85 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q5ZIW1

Entry ID Method Resolution Chain Position Source
AF-Q5ZIW1-F1 Predicted AlphaFoldDB

No variants for Q5ZIW1

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q5ZIW1

No associated diseases with Q5ZIW1

2 regional properties for Q5ZIW1

Type Name Position InterPro Accession
domain DNA helicase, DnaB-like, C-terminal 390 - 641 IPR007694
domain Archaeal primase DnaG/twinkle-like, TOPRIM domain 265 - 341 IPR034154

Functions

Description
EC Number 5.6.2.3 Enzymes altering nucleic acid conformation
Subcellular Localization
  • Mitochondrion matrix, mitochondrion nucleoid
  • Mitochondrion inner membrane ; Peripheral membrane protein
  • Colocalizes with mtDNA in mitochondrial nucleoids, a nucleoproteins complex consisting of a number of copies of proteins associated with mtDNA, probably involved in mtDNA maintenance and expression (By similarity)
  • Associates with phospholipid membranes via electrostatic binding (By similarity)
  • Preferentially associates with membranes enriched with cardiolipin, a lipid abundant in the mitochondrial inner membrane (By similarity)
  • ATPase and helicase activity is enhanced by binding to lipid membranes (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
mitochondrial nucleoid The region of a mitochondrion to which the DNA is confined.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

5 GO annotations of molecular function

Name Definition
5'-3' DNA helicase activity Unwinding a DNA helix in the 5' to 3' direction, driven by ATP hydrolysis.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
DNA helicase activity Unwinding of a DNA helix, driven by ATP hydrolysis.
single-stranded DNA binding Binding to single-stranded DNA.

1 GO annotations of biological process

Name Definition
mitochondrial DNA replication The process in which new strands of DNA are synthesized in the mitochondrion.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q96RR1 TWNK Twinkle mtDNA helicase Homo sapiens (Human) PR
Q8CIW5 Twnk Twinkle mtDNA helicase Mus musculus (Mouse) PR
B5X582 At1g30680 Twinkle homolog protein, chloroplastic/mitochondrial Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MAAVPLRPCR ASGRFLPLLR GGARSRGASP WAAGPGRVAQ RRYKKDVLPE PGGAAPAVSV
70 80 90 100 110 120
TEIRRYLRAQ GIPFQDGYSC LHAPSLFGPE LPPAAGPPFT LFIDKTTGSF LCTATLAEGG
130 140 150 160 170 180
WQDLQAAVEL RHRGVPAPGP DEGEREEEEE AEARRAREDA RRIWERALPL GELLDEEETR
190 200 210 220 230 240
ATKAAFGIAP LADGTLKRFG VRYLRAAKAL VFPWFAPRDA ALRGLKLLVA EQRGDAVSYT
250 260 270 280 290 300
EETLPRFDAY RNLFGLPLIG RRDAEVVLTG SELDALALHQ ATGVPCLALP RGATILPPAL
310 320 330 340 350 360
LPYLEQFRRV TLWLGDDLRS WEASKLFARK LNPKRCSLVQ PGDLQPRPLE ALNRGLNLTK
370 380 390 400 410 420
ILRAALPAGH KAIVSFRQLR EEVFGELANS EQVAGVKWAR FPELNKLLKG HRRGELTVFT
430 440 450 460 470 480
GPTGSGKTTF ISEYALDLCT QGVCTLWGSF EINNIRLAKI MLTQFATRRL EDQLELYDEW
490 500 510 520 530 540
ADRFEDLPLY FMTFHGQQNI KTVLDTMQHA VYMYDITHVV VDNLQFMMGH EQLSADRLAA
550 560 570 580 590 600
QDFIVGAFRK FATDNTCHIT LVIHPRKEDD EKELQTASIF GSAKASQEAD NVLILQDRKL
610 620 630 640 650 660
TTGPGKRYLQ VSKNRFDGDV GVFPLEFSKA SLTFSSYGKS KAKLKKVKEE KGPSPKKGPS
GGVGGSSKP