B5X582

PR
Gene name
At1g30680 (T5I8.13)
Protein name
Twinkle homolog protein, chloroplastic/mitochondrial
Names
DNA helicase, DNA primase
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT1G30680
EC number
3.6.4.12: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

11-162 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for B5X582

Entry ID Method Resolution Chain Position Source
AF-B5X582-F1 Predicted AlphaFoldDB

34 variants for B5X582

Variant ID(s) Position Change Description Diseaes Association Provenance
tmp_1_10881673_T_G,C 3 F>L No 1000Genomes
ENSVATH14013142 4 L>F No 1000Genomes
ENSVATH04678110 13 R>Q No 1000Genomes
tmp_1_10881762_T_C 33 L>P No 1000Genomes
ENSVATH12407548 57 R>I No 1000Genomes
ENSVATH14013143 58 R>S No 1000Genomes
tmp_1_10881845_C_T 61 P>S No 1000Genomes
tmp_1_10881849_T_A 62 V>D No 1000Genomes
ENSVATH04678113 62 V>I No 1000Genomes
ENSVATH14013144 77 R>K No 1000Genomes
ENSVATH14013155 79 N>S No 1000Genomes
ENSVATH12407549 86 S>L No 1000Genomes
tmp_1_10881923_A_T 87 I>F No 1000Genomes
tmp_1_10882247_C_T 117 A>V No 1000Genomes
ENSVATH14013157 125 N>K No 1000Genomes
ENSVATH00052507 131 H>Y No 1000Genomes
tmp_1_10882302_A_G 135 I>M No 1000Genomes
ENSVATH01131154 172 K>T No 1000Genomes
ENSVATH04678120 177 A>V No 1000Genomes
tmp_1_10882767_G_A 178 D>N No 1000Genomes
ENSVATH01131159 180 G>R No 1000Genomes
tmp_1_10882803_G_A 190 V>I No 1000Genomes
tmp_1_10882839_G_A 202 E>K No 1000Genomes
ENSVATH01131168 209 Q>R No 1000Genomes
ENSVATH04678126 228 M>L No 1000Genomes
ENSVATH00052515 265 K>M No 1000Genomes
ENSVATH01131177 282 V>I No 1000Genomes
tmp_1_10883698_A_C 294 M>L No 1000Genomes
ENSVATH04678130 315 S>L No 1000Genomes
tmp_1_10884060_C_T 357 A>V No 1000Genomes
tmp_1_10884125_G_A 379 E>K No 1000Genomes
tmp_1_10884959_T_C 524 V>A No 1000Genomes
tmp_1_10885555_A_T 655 N>Y No 1000Genomes
ENSVATH12408156 695 P>S No 1000Genomes

No associated diseases with B5X582

3 regional properties for B5X582

Type Name Position InterPro Accession
domain TOPRIM domain 280 - 372 IPR006171
domain DNA helicase, DnaB-like, C-terminal 430 - 698 IPR007694
domain Archaeal primase DnaG/twinkle-like, TOPRIM domain 281 - 372 IPR034154

Functions

Description
EC Number 3.6.4.12 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Plastid, chloroplast
  • Mitochondrion
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
chloroplast A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

7 GO annotations of molecular function

Name Definition
5'-3' DNA helicase activity Unwinding a DNA helix in the 5' to 3' direction, driven by ATP hydrolysis.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
DNA helicase activity Unwinding of a DNA helix, driven by ATP hydrolysis.
DNA primase activity Catalysis of the synthesis of a short RNA primer on a DNA template, providing a free 3'-OH that can be extended by DNA-directed DNA polymerases.
metal ion binding Binding to a metal ion.
single-stranded DNA binding Binding to single-stranded DNA.

1 GO annotations of biological process

Name Definition
ribonucleotide biosynthetic process The chemical reactions and pathways resulting in the formation of a ribonucleotide, a compound consisting of ribonucleoside (a base linked to a ribose sugar) esterified with a phosphate group at either the 3' or 5'-hydroxyl group of the sugar.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q5ZIW1 TWNK Twinkle mtDNA helicase Gallus gallus (Chicken) PR
Q96RR1 TWNK Twinkle mtDNA helicase Homo sapiens (Human) PR
Q8CIW5 Twnk Twinkle mtDNA helicase Mus musculus (Mouse) PR
10 20 30 40 50 60
MRFLLRLPQI HFRKLSCSMS VLMGSKQFLE FCLLPSFASY PSSPSYSSSR QVSSVSRRFR
70 80 90 100 110 120
PVLASRPVSK NSPYYQRTNG LSSYNSIPRV PTPVDTEVEA DKRVVLSRLV TLRRKLAEQG
130 140 150 160 170 180
VDAENCPPGQ HSGLICPTCE GGNSGEKSLS LFIAPDGSSA TWNCFRGKCG LKGGVRADGG
190 200 210 220 230 240
LASADPIEKV ERKITVEGIE LEPLCDEIQD YFAARAISRK TLERNRVMQK RIGDEIVIAF
250 260 270 280 290 300
TYWQRGELVS CKYRSLTKMF FQERKTRRIL YGLDDIEKTS EVIIVEGEID KLAMEEAGFL
310 320 330 340 350 360
NCVSVPDGAP AKVSSKEIPS EDKDTKYKFL WNCNDYLKKA SRIVIATDGD GPGQAMAEEI
370 380 390 400 410 420
ARRLGKERCW RVKWPKKSED EHFKDANEVL MSKGPHLLKE AILDAEPYPI LGLFSFKDFF
430 440 450 460 470 480
DEIDAYYDRT HGHEYGVSTG WKNLDNLYSV VPGELTVVTG IPNSGKSEWI DAMLCNLNHS
490 500 510 520 530 540
VGWKFALCSM ENKVRDHARK LLEKHIKKPF FDADYGRSVQ RMSVEEKDEG KKWLNDTFYP
550 560 570 580 590 600
IRCEMDSLPS IDWVLERAKA AVLRYGIRGL VIDPYNELDH QRTPRQTETE YVSQMLTKIK
610 620 630 640 650 660
RFSQHHSCHV WFVAHPKQLQ HWDGGAPNLY DISGSAHFIN KCDNGIIVHR NRDENAGPLD
670 680 690 700
LVQIGVRKVR NKVAGQIGDA YLCYDRTTGS YSDSPVTPGM PERRSPKRY