Q5HZJ0

PR
Gene name
Drosha (Etohi2, Rn3, Rnasen)
Protein name
Ribonuclease 3
Names
Protein Drosha, Ribonuclease III, RNase III
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:14000
EC number
3.1.26.3: Endoribonucleases producing 5'-phosphomonoesters
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-462 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q5HZJ0

Entry ID Method Resolution Chain Position Source
AF-Q5HZJ0-F1 Predicted AlphaFoldDB

60 variants for Q5HZJ0

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3389307387 68 P>H No EVA
rs3405783635 175 F>S No EVA
rs262625945 266 D>E No EVA
rs3389325161 290 R>S No EVA
rs3389336806 317 Y>* No EVA
rs31936126 320 P>S No EVA
rs241505903 330 P>A No EVA
rs3405906712 356 S>C No EVA
rs3405681063 357 R>M No EVA
rs3405845732 357 R>W No EVA
rs228110127 382 N>T No EVA
rs219409109 388 E>D No EVA
rs228498905 394 V>A No EVA
rs3389325106 395 P>L No EVA
rs212801796 401 E>Q No EVA
rs231394879 403 E>D No EVA
rs3389343689 409 P>S No EVA
rs3389317143 463 P>H No EVA
rs3389275205 473 D>N No EVA
rs3389336781 474 E>D No EVA
rs1131910898 500 V>G No EVA
rs3389369294 514 P>S No EVA
rs3389343756 538 S>N No EVA
rs3389325090 558 K>M No EVA
rs3389317148 573 Y>F No EVA
rs3389352704 604 S>Y No EVA
rs3389348672 618 K>I No EVA
rs3389343743 656 L>M No EVA
rs3389348647 683 R>C No EVA
rs3405885175 698 M>R No EVA
rs263529557 701 I>V No EVA
rs3405981822 711 A>V No EVA
rs3406139938 714 P>H No EVA
rs3405776872 715 E>K No EVA
rs3405847308 716 E>D No EVA
rs3404995199 717 E>K No EVA
rs3389348657 791 K>N No EVA
rs3389336788 791 K>R No EVA
rs3389369346 794 K>N No EVA
rs3389360369 803 L>I No EVA
rs3389356623 819 Q>K No EVA
rs3389275200 968 E>K No EVA
rs3389348693 985 Y>* No EVA
rs3405982724 992 E>A No EVA
rs3389317155 992 E>D No EVA
rs3389307456 993 G>R No EVA
rs3405784644 1002 I>N No EVA
rs3389275167 1032 E>K No EVA
rs3389275167 1032 E>Q No EVA
rs3389307396 1057 E>* No EVA
rs3389343693 1158 T>I No EVA
rs3389348636 1178 S>R No EVA
rs3389325144 1193 L>R No EVA
rs3389356515 1195 M>V No EVA
rs3389356681 1220 L>M No EVA
rs3389356659 1233 L>P No EVA
rs3389356644 1242 V>I No EVA
rs3389356486 1246 P>T No EVA
rs3389307383 1282 L>I No EVA
rs3389364751 1293 H>Q No EVA

No associated diseases with Q5HZJ0

6 regional properties for Q5HZJ0

Type Name Position InterPro Accession
domain Rab-GAP-TBC domain 505 - 718 IPR000195
domain EF-hand domain 879 - 914 IPR002048
domain GRAM domain 142 - 248 IPR004182-1
domain GRAM domain 288 - 396 IPR004182-2
domain TCB1D9/TCB1D9B, PH-GRAM domain 1 153 - 251 IPR036014
domain TCB1D9/TCB1D9B, PH-GRAM domain 2 299 - 394 IPR036017

Functions

Description
EC Number 3.1.26.3 Endoribonucleases producing 5'-phosphomonoesters
Subcellular Localization
  • Nucleus
  • Nucleus, nucleolus
  • A fraction is translocated to the nucleolus during the S phase of the cell cycle
  • Localized in GW bodies (GWBs), also known as P-bodies
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
microprocessor complex A protein complex that binds to heme and to pri-miRNAs, and is required for the formation of a pre-microRNA (pre-miRNA), the initial step of microRNA (miRNA) biogenesis. The complex is composed of the double-stranded-RNA-specific RNase Drosha (also called RNASEN) and the RNA-binding protein DGCR8 (heme-free or heme-bound forms). Within the complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs RNASEN/Drosha to cleave the 3' and 5' strands of a stem-loop to release hairpin-shaped pre-miRNAs.
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
postsynaptic density An electron dense network of proteins within and adjacent to the postsynaptic membrane of an asymmetric, neuron-neuron synapse. Its major components include neurotransmitter receptors and the proteins that spatially and functionally organize them such as anchoring and scaffolding molecules, signaling enzymes and cytoskeletal components.

10 GO annotations of molecular function

Name Definition
DEAD/H-box RNA helicase binding Binding to a DEAD/H-box RNA helicase.
double-stranded RNA binding Binding to double-stranded RNA.
endoribonuclease activity Catalysis of the hydrolysis of ester linkages within ribonucleic acid by creating internal breaks.
lipopolysaccharide binding Binding to a lipopolysaccharide.
metal ion binding Binding to a metal ion.
primary miRNA binding Binding to a primary microRNA (pri-miRNA) transcript, an RNA molecule that is processed into a short hairpin-shaped structure called a pre-miRNA and finally into a functional miRNA. Both double-stranded and single-stranded regions of a pri-miRNA are required for binding.
protein homodimerization activity Binding to an identical protein to form a homodimer.
R-SMAD binding Binding to a receptor-regulated SMAD signaling protein.
ribonuclease III activity Catalysis of the endonucleolytic cleavage of RNA with 5'-phosphomonoesters and 3'-OH termini; makes two staggered cuts in both strands of dsRNA, leaving a 3' overhang of 2 nt.
SMAD binding Binding to a SMAD signaling protein.

12 GO annotations of biological process

Name Definition
defense response to Gram-negative bacterium Reactions triggered in response to the presence of a Gram-negative bacterium that act to protect the cell or organism.
defense response to Gram-positive bacterium Reactions triggered in response to the presence of a Gram-positive bacterium that act to protect the cell or organism.
miRNA metabolic process The chemical reactions and pathways involving miRNA, microRNA, a class of single-stranded RNA molecules of about 21-23 nucleotides in length, which regulates gene expression.
positive regulation of gene expression Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
pre-miRNA processing A process involved in the conversion of a pre-microRNA transcript into a mature microRNA molecule.
primary miRNA processing A process involved in the conversion of a primary microRNA transcript into a pre-microRNA molecule.
regulation of gene expression Any process that modulates the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
regulation of inflammatory response Any process that modulates the frequency, rate or extent of the inflammatory response, the immediate defensive reaction (by vertebrate tissue) to infection or injury caused by chemical or physical agents.
regulation of miRNA metabolic process Any process that modulates the frequency, rate or extent of miRNA metabolic process.
regulation of regulatory T cell differentiation Any process that modulates the frequency, rate or extent of differentiation of regulatory T cells.
RNA processing Any process involved in the conversion of one or more primary RNA transcripts into one or more mature RNA molecules.
rRNA processing Any process involved in the conversion of a primary ribosomal RNA (rRNA) transcript into one or more mature rRNA molecules.

1 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9NRR4 DROSHA Ribonuclease 3 Homo sapiens (Human) PR
10 20 30 40 50 60
MQGNTCHRMS YHPGRGCPRG RGGHGARPSA PAFRPQNLRL LHPQQPPAQY QYEPPSAPSS
70 80 90 100 110 120
SYSNSQAPSF MPPRPDFVPY PPPAAPSAQG PLPPCPVRPP YPNHQMRHPF PVPPCFPPMP
130 140 150 160 170 180
PPMPCPNNPP ASGAPPGQGT FPFMVPPPSM PHPPPPPVMP QQVNYQYPPG YSHSFPPPGF
190 200 210 220 230 240
NSYQNNSSSF PPSANSSSTP HFRHLPPYSL PKAQNERRSP ERLKHYDDHR HRDHSHGRGE
250 260 270 280 290 300
RHRSLERRER GRSPERRRPE SRYRSDYDRG RTPPPRHRSY ERSRERDRER HRHREARRSP
310 320 330 340 350 360
SLERSYKKEY KRSGRSYALP VAPEPAGCTP ELPGEMIKTT ESWAPPPENV NHRSPSREKK
370 380 390 400 410 420
RARWEEEKDR WSDSQGSGKE KNYTSIKEKE AEEVPPEKTE EEEEELLKPV WIRCTHSESY
430 440 450 460 470 480
YSSDPMDQVG DSTVVGTSRL RDLYDKFEEE LGNRQEKAKA ARPPWEPPKT KLDEDLESSS
490 500 510 520 530 540
ESECETDDDS TCSSSSDSEV FDVIAEIKRK KAHPDRLHDE LWYNDPGQMN DGPLCKCSAK
550 560 570 580 590 600
ARRTGIRHSI YPGEEAIKPC RPMTNNAGRL FHYRITVSPP TNFLTDRPTV IEYDDHEYIF
610 620 630 640 650 660
EGFSMFAHAP LTNIPLCKVI RFNIDYTIHF IEEMMPENFC VKGLELFSLF LFRDILELYD
670 680 690 700 710 720
WNLKGPLFED SPPCCPRFHF MPRFVRFLPD GGKEVLSMHQ ILLYLLRCSK ALVPEEEIAN
730 740 750 760 770 780
MLQWEELEWQ KYAEECKGMI VTNPGTKPSS VRIDQLDREQ FNPEVITFPI IVHFGIRPAQ
790 800 810 820 830 840
LSYAGDPQYQ KLWKSYVKLR HLLANSPKVK QTDKQKLAQR EEALQKIRQK NTMRREVTVE
850 860 870 880 890 900
LSSQGFWKTG IRSDVCQHAM MLPVLTHHIR YHQCLMHLDK LIGYTFQDRC LLQLAMTHPS
910 920 930 940 950 960
HHLNFGMNPD HARNSLSNCG IRQPKYGDRK VHHMHMRKKG INTLINIMSR LGQDDPTPSR
970 980 990 1000 1010 1020
INHNERLEFL GDAVVEFLTS VHLYYLFPSL EEGGLATYRT AIVQNQHLAM LAKKLELDRF
1030 1040 1050 1060 1070 1080
MLYAHGPDLC RESDLRHAMA NCFEALIGAV YLEGSLEEAK QLFGRLLFND PDLREVWLNY
1090 1100 1110 1120 1130 1140
PLHPLQLQEP NTDRQLIETS PVLQKLTEFE EAIGVIFTHV RLLARAFTLR TVGFNHLTLG
1150 1160 1170 1180 1190 1200
HNQRMEFLGD SIMQLVATEY LFIHFPDHHE GHLTLLRSSL VNNRTQAKVA EELGMQEYAI
1210 1220 1230 1240 1250 1260
TNDKTKRPVA LRTKTLADLL ESFIAALYID KDLEYVHTFM NVCFFPRLKE FILNQDWNDP
1270 1280 1290 1300 1310 1320
KSQLQQCCLT LRTEGKEPDI PLYKTLQTVG PSHARTYTVA VYFKGERIGC GKGPSIQQAE
1330 1340 1350 1360 1370
MGAAMDALEK YNFPQMAHQK RFIERKYRQE LKEMRWEREH QEREPEEAED IKK