AiPD: Autoinhibited Protein Database

Q01658

Gene name	DR1
Protein name	Protein Dr1
Names	Down-regulator of transcription 1, Negative cofactor 2-beta, NC2-beta, TATA-binding protein-associated phosphoprotein
Species	Homo sapiens (Human)
KEGG Pathway	hsa:1810
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

65-176 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

65-176 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

2 structures for Q01658

Entry ID	Method	Resolution	Chain	Position	Source
1JFI	X-ray	262 A	B	1-176	PDB
AF-Q01658-F1	Predicted				AlphaFoldDB

74 variants for Q01658

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs1219975772 CA341358372	2	A>S		No	ClinGen gnomAD
rs756787813 CA954787	3	S>F		No	ClinGen ExAC gnomAD
rs780919729 CA954788	4	S>L	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
TCGA novel	7	N>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs867908890 CA27143179	10	D>E		No	ClinGen Ensembl
rs1469325614 CA341358537	13	I>M		No	ClinGen gnomAD
rs894557660 CA27143180	13	I>V		No	ClinGen TOPMed
TCGA novel	15	R>E	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs779711357 CA954791	15	R>S		No	ClinGen ExAC gnomAD
rs1415552231 CA341358585	17	A>T		No	ClinGen TOPMed
CA341358617 rs1162505520	19	N>S		No	ClinGen TOPMed
CA27143182 rs1004346820	21	M>V		No	ClinGen TOPMed
TCGA novel	24	E>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA341358705 rs1430846443	25	T>A		No	ClinGen gnomAD
CA954793 rs749283246	25	T>I		No	ClinGen ExAC TOPMed gnomAD
CA954792 rs749283246	25	T>S		No	ClinGen ExAC TOPMed gnomAD
rs1401660944 CA341358714	26	L>F		No	ClinGen gnomAD
CA341358732 rs1412070666	27	P>A		No	ClinGen gnomAD
CA341358740 rs1423545058	28	N>D		No	ClinGen gnomAD
rs1045693074 CA27143183	28	N>S		No	ClinGen Ensembl
CA27143184 rs866893831	30	R>L		No	ClinGen Ensembl
rs907234002 CA27143185	31	V>L		No	ClinGen Ensembl
rs901374287 CA27143186	34	D>G		No	ClinGen TOPMed
TCGA novel	35	A>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	36	R>L	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA954796 rs372870592	41	N>S		No	ClinGen ExAC TOPMed gnomAD
CA27143187 rs34893851	44	T>A		No	ClinGen Ensembl
rs752063632 CA954803	50	I>T		No	ClinGen ExAC gnomAD
CA954802 rs764726619	50	I>V	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
rs1354541937 CA341359095	54	A>G		No	ClinGen TOPMed gnomAD
CA27143190 rs1028595390	59	N>Y		No	ClinGen TOPMed
rs761400080 CA954804	61	S>L		No	ClinGen ExAC gnomAD
rs1032345601 CA27143191	66	I>M		No	ClinGen Ensembl
CA341359286 rs1479210475	69	E>G		No	ClinGen TOPMed gnomAD
CA341359287 rs1479210475	69	E>V		No	ClinGen TOPMed gnomAD
rs1198074928 CA341359294	70	H>N		No	ClinGen gnomAD
CA954806 rs535394702	72	I>V		No	ClinGen 1000Genomes ExAC gnomAD
CA341359828 rs1570710771	74	A>G		No	ClinGen Ensembl
rs1218729159 CA341359844	77	S>G		No	ClinGen TOPMed
rs753443505 CA954826	81	G>D		No	ClinGen ExAC gnomAD
CA341359874 rs1341789506	81	G>S		No	ClinGen TOPMed
CA954827 rs754785474	82	S>F		No	ClinGen ExAC TOPMed gnomAD
TCGA novel	85	S>G	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA341359939 rs1316923518	90	V>A		No	ClinGen TOPMed gnomAD
CA954830 rs758243363	93	E>D		No	ClinGen ExAC gnomAD
TCGA novel	94	C>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs781371064 CA954831	94	C>Y		No	ClinGen ExAC gnomAD
TCGA novel	95	K>I	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA954833 rs756169054	100	K>R		No	ClinGen ExAC gnomAD
CA341360039 rs1462169983	105	S>C		No	ClinGen gnomAD
CA341360043 rs1185919174	105	S>I		No	ClinGen gnomAD
CA27143932 rs932303992	107	R>C		No	ClinGen TOPMed
rs749454147 CA954835	107	R>H		No	ClinGen ExAC gnomAD
TCGA novel	116	E>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs200893076 CA27143933	126	F>L		No	ClinGen Ensembl
rs1250766935 CA341360237	131	Q>*		No	ClinGen TOPMed
rs1224817375 CA341360256	133	Q>L		No	ClinGen gnomAD
CA954860 rs747487257	135	E>G		No	ClinGen ExAC gnomAD
COSM913395 CA954859 rs182894486	135	E>K	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated 1000Genomes ESP ExAC NCI-TCGA TOPMed gnomAD
CA341360284 rs1187530213	138	Q>K		No	ClinGen TOPMed gnomAD
CA341360317 rs1204239503	142	L>F		No	ClinGen TOPMed
CA954864 rs764897702	144	M>T		No	ClinGen ExAC gnomAD
CA27144616 rs200292726	149	Q>H		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs763881675 CA954867	150	Q>E		No	ClinGen ExAC gnomAD
CA341360379 rs1254062824	150	Q>H		No	ClinGen TOPMed
rs1232222631 CA341360386	151	A>V		No	ClinGen TOPMed
rs768881891 CA954868	156	A>T		No	ClinGen ExAC TOPMed gnomAD
rs1356108234 CA341360421	157	S>L		No	ClinGen gnomAD
rs1309410753 CA341360448	161	S>C		No	ClinGen TOPMed
rs375320336 CA954869	164	A>V	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen 1000Genomes ExAC NCI-TCGA TOPMed gnomAD
CA341360497 rs1280841194	169	D>N		No	ClinGen gnomAD
CA954872 rs3088371 VAR_034506	171	E>D		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA341360532 rs1302682132	173	D>G		No	ClinGen TOPMed
rs1298347559 CA341360548	175	D>G		No	ClinGen gnomAD

No associated diseases with Q01658

4 regional properties for Q01658

Type	Name	Position	InterPro Accession
domain	Tudor domain	4 - 62	IPR002999
conserved_site	Sterol reductase, conserved site	362 - 377	IPR018083-1
conserved_site	Sterol reductase, conserved site	579 - 602	IPR018083-2
domain	Lamin-B receptor of TUDOR domain	1 - 56	IPR019023

Functions

		Description
EC Number
Subcellular Localization	Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

5 GO annotations of cellular component

Name	Definition
ATAC complex	A chromatin remodelling complex that regulates transcription via acetylation primarily of nucleosomal histones H3 and possibly H4. Shares the histone acetylation (HAT) module of GCN5/PCAF-ADA2-ADA3-SGF29 (or orthologs) with the related SAGA complex (GO:0000124). Contains HAT subunits GCN5 or PCAF in a mutually exclusive manner. In addition to the HAT module contains DR1/NC2B, KAT14, MBIP, WDR5, YEATS2 and ZZZ3 or orthologs. Also regulates the activity of non-histone targets and orchestrates mitotic progression by regulating Cyclin A degradation through acetylation.
mitotic spindle	A spindle that forms as part of mitosis. Mitotic and meiotic spindles contain distinctive complements of proteins associated with microtubules.
negative cofactor 2 complex	A heterodimeric protein complex that can stably associate with TATA-binding protein on promoters, thereby preventing the assembly of transcription factors TFIIA and TFIIB and leading to repression of RNA polymerase II transcription. The two subunits, NC2alpha (Drap1) and NC2beta (Dr1), dimerize through histone fold domains of the H2A/H2B type present in the amino termini.
nucleoplasm	That part of the nuclear content other than the chromosomes or the nucleolus.
RNA polymerase II transcription regulator complex	A transcription factor complex that acts at a regulatory region of a gene transcribed by RNA polymerase II.

4 GO annotations of molecular function

Name	Definition
core promoter sequence-specific DNA binding	Binding to a sequence of DNA that is part of a core promoter region. The core promoter is composed of the transcription start site and binding sites for the RNA polymerase and the basal transcription machinery. The transcribed region might be described as a gene, cistron, or operon.
protein heterodimerization activity	Binding to a nonidentical protein to form a heterodimer.
RNA polymerase II general transcription initiation factor activity	A general transcription initiation factor activity that contributes to transcription start site selection and transcription initiation of genes transcribed by RNA polymerase II. The general transcription factors for RNA polymerase II include TFIIB, TFIID, TFIIE, TFIIF, TFIIH and TATA-binding protein (TBP). In most species, RNA polymerase II transcribes all messenger RNAs (mRNAs), most untranslated regulatory RNAs, the majority of the snoRNAs, four of the five snRNAs (U1, U2, U4, and U5), and other small noncoding RNAs. For some small RNAs there is variability between species as to whether it is transcribed by RNA polymerase II or RNA polymerase III. However there are also rare exceptions, such as Trypanosoma brucei, where RNA polymerase I transcribes certain mRNAs in addition to its normal role in rRNA transcription.
TBP-class protein binding	Binding to a member of the class of TATA-binding proteins (TBP), including any of the TBP-related factors (TRFs).

13 GO annotations of biological process

Name	Definition
chromatin remodeling	A dynamic process of chromatin reorganization resulting in changes to chromatin structure. These changes allow DNA metabolic processes such as transcriptional regulation, DNA recombination, DNA repair, and DNA replication.
histone H3 acetylation	The modification of histone H3 by the addition of an acetyl group.
histone H3-K14 acetylation	The modification of histone H3 by the addition of an acetyl group to a lysine residue at position 14 of the histone.
negative regulation of transcription by RNA polymerase II	Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
positive regulation of transcription by RNA polymerase II	Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
regulation of cell cycle	Any process that modulates the rate or extent of progression through the cell cycle.
regulation of cell division	Any process that modulates the frequency, rate or extent of the physical partitioning and separation of a cell into daughter cells.
regulation of DNA-templated transcription	Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
regulation of embryonic development	Any process that modulates the frequency, rate or extent of embryonic development.
regulation of histone deacetylation	Any process that modulates the frequency, rate or extent of the removal of acetyl groups from histones.
regulation of transcription by RNA polymerase II	Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
regulation of tubulin deacetylation	Any process that modulates the frequency, rate or extent of tubulin deacetylation. Tubulin deacetylation is the removal of an acetyl group from a protein amino acid.
RNA polymerase II preinitiation complex assembly	The aggregation, arrangement and bonding together of proteins on an RNA polymerase II promoter DNA to form the transcriptional preinitiation complex (PIC), the formation of which is a prerequisite for transcription by RNA polymerase.

3 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q92317	NCB2	Negative cofactor 2 complex subunit beta	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	PR
Q91WV0	Dr1	Protein Dr1	Mus musculus (Mouse)	PR
Q5XI68	Dr1	Protein Dr1	Rattus norvegicus (Rat)	PR

10	20	30	40	50	60
MASSSGNDDD	LTIPRAAINK	MIKETLPNVR	VANDARELVV	NCCTEFIHLI	SSEANEICNK
70	80	90	100	110	120
SEKKTISPEH	VIQALESLGF	GSYISEVKEV	LQECKTVALK	RRKASSRLEN	LGIPEEELLR
130	140	150	160	170
QQQELFAKAR	QQQAELAQQE	WLQMQQAAQQ	AQLAAASASA	SNQAGSSQDE	EDDDDI