P97467

PR
Gene name
Pam
Protein name
Peptidyl-glycine alpha-amidating monooxygenase
Names
PAM
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:18484
EC number
1.14.17.3: With reduced ascorbate as one donor, and incorporation of one atom of oxygen
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

913-979 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P97467

Entry ID Method Resolution Chain Position Source
AF-P97467-F1 Predicted AlphaFoldDB

68 variants for P97467

Variant ID(s) Position Change Description Diseaes Association Provenance
rs249638524 50 T>I No EVA
rs3388492486 93 V>M No EVA
rs3388490175 102 M>T No EVA
rs3388493548 108 M>I No EVA
rs3388491779 130 C>Y No EVA
rs3388491004 147 T>P No EVA
rs3388491471 251 G>D No EVA
rs3388491471 251 G>DG* No EVA
rs3388492024 252 Y>* No EVA
rs3388491741 254 V>A No EVA
rs3388494344 259 W>* No EVA
rs3388490189 260 T>R No EVA
rs3388491718 262 I>V No EVA
rs3388494311 286 D>N No EVA
rs219176258 293 V>M No EVA
rs3388492319 297 E>V No EVA
rs3388494355 319 M>I No EVA
rs3388491989 330 F>Y No EVA
rs3388491793 332 T>I No EVA
rs222669691 388 A>V No EVA
rs3388490634 415 N>K No EVA
rs8239087 419 K>R No EVA
rs38444704 433 V>I No EVA
rs225976582 448 A>T No EVA
rs8239088 450 H>R No EVA
rs3388494485 484 Q>H No EVA
rs8239089 484 Q>R No EVA
rs3388490578 486 P>S No EVA
rs36911792 491 W>R No EVA
rs214058782 495 L>P No EVA
rs3388492528 551 Q>* No EVA
rs3388489043 574 Q>R No EVA
rs3388491415 581 F>E No EVA
rs3388489020 582 Y>S No EVA
rs3388490546 610 P>S No EVA
rs215275920 611 R>H No EVA
rs3388494474 614 E>D No EVA
rs252078021 626 P>A No EVA
rs3412803299 636 P>T No EVA
rs3390367113 638 D>S No EVA
rs3388490635 640 A>T No EVA
rs36292592 642 E>D No EVA
rs3388494481 650 V>M No EVA
rs3388491560 656 N>S No EVA
rs36321707 660 V>M No EVA
rs8239091 664 P>L No EVA
rs3388489092 680 S>G No EVA
rs3388491945 688 V>D No EVA
rs3412985799 694 L>H No EVA
rs3388491592 722 V>A No EVA
rs3388490221 748 N>H No EVA
rs3388492052 789 V>L No EVA
rs3388494381 793 D>H No EVA
rs3388490279 796 V>M No EVA
rs3388491430 811 L>P No EVA
rs3388492542 811 L>V No EVA
rs3388490459 812 T>N No EVA
rs3388492533 824 K>R No EVA
rs3388489021 825 K>T No EVA
rs3388492379 854 Q>* No EVA
rs3388490305 862 L>M No EVA
rs3388490561 865 D>Y No EVA
rs3388490330 877 T>I No EVA
rs3388490478 900 A>V No EVA
rs3388489088 906 R>L No EVA
rs259401642 919 L>F No EVA
rs3388490325 944 F>I No EVA
rs3388494374 950 E>G No EVA

No associated diseases with P97467

8 regional properties for P97467

Type Name Position InterPro Accession
domain Copper type II, ascorbate-dependent monooxygenase, N-terminal 64 - 175 IPR000323
repeat NHL repeat 570 - 611 IPR001258-1
repeat NHL repeat 620 - 665 IPR001258-2
repeat NHL repeat 673 - 717 IPR001258-3
repeat NHL repeat 769 - 812 IPR001258-4
conserved_site Copper type II, ascorbate-dependent monooxygenase, histidine-cluster-2 conserved site 234 - 246 IPR014783
conserved_site Copper type II, ascorbate-dependent monooxygenase, histidine-cluster-1 conserved site 106 - 113 IPR020611
domain Copper type II ascorbate-dependent monooxygenase, C-terminal 201 - 344 IPR024548

Functions

Description
EC Number 1.14.17.3 With reduced ascorbate as one donor, and incorporation of one atom of oxygen
Subcellular Localization
  • Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein
  • Secretory granules
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

14 GO annotations of cellular component

Name Definition
cell surface The external part of the cell wall and/or plasma membrane.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
extracellular region The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
extracellular space That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid.
integral component of plasma membrane The component of the plasma membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
neuron projection A prolongation or process extending from a nerve cell, e.g. an axon or dendrite.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
perikaryon The portion of the cell soma (neuronal cell body) that excludes the nucleus.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
secretory granule A small subcellular vesicle, surrounded by a membrane, that is formed from the Golgi apparatus and contains a highly concentrated protein destined for secretion. Secretory granules move towards the periphery of the cell and upon stimulation, their membranes fuse with the cell membrane, and their protein load is exteriorized. Processing of the contained protein may take place in secretory granules.
secretory granule membrane The lipid bilayer surrounding a secretory granule.
trans-Golgi network The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side distal to the endoplasmic reticulum, from which secretory vesicles emerge. The trans-Golgi network is important in the later stages of protein secretion where it is thought to play a key role in the sorting and targeting of secreted proteins to the correct destination.
transport vesicle membrane The lipid bilayer surrounding a transport vesicle.

8 GO annotations of molecular function

Name Definition
calcium ion binding Binding to a calcium ion (Ca2+).
copper ion binding Binding to a copper (Cu) ion.
identical protein binding Binding to an identical protein or proteins.
L-ascorbic acid binding Binding to L-ascorbic acid, (2R)-2-[(1S)-1,2-dihydroxyethyl]-4-hydroxy-5-oxo-2,5-dihydrofuran-3-olate; L-ascorbic acid is vitamin C and has co-factor and anti-oxidant activities in many species.
peptidylamidoglycolate lyase activity Catalysis of the reaction: peptidylamidoglycolate = peptidyl amide + glyoxylate.
peptidylglycine monooxygenase activity Catalysis of the reaction: peptidyl-glycine + ascorbate + O2 = peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
zinc ion binding Binding to a zinc ion (Zn).

24 GO annotations of biological process

Name Definition
central nervous system development The process whose specific outcome is the progression of the central nervous system over time, from its formation to the mature structure. The central nervous system is the core nervous system that serves an integrating and coordinating function. In vertebrates it consists of the brain and spinal cord. In those invertebrates with a central nervous system it typically consists of a brain, cerebral ganglia and a nerve cord.
fatty acid primary amide biosynthetic process The chemical reactions and pathways resulting in the formation of a fatty acid primary amide.
heart development The process whose specific outcome is the progression of the heart over time, from its formation to the mature structure. The heart is a hollow, muscular organ, which, by contracting rhythmically, keeps up the circulation of the blood.
lactation The regulated release of milk from the mammary glands and the period of time that a mother lactates to feed her young.
limb development The process whose specific outcome is the progression of a limb over time, from its formation to the mature structure. A limb is an appendage of an animal used for locomotion or grasping. Examples include legs, arms or some types of fin.
long-chain fatty acid metabolic process The chemical reactions and pathways involving long-chain fatty acids, A long-chain fatty acid is a fatty acid with a chain length between C13 and C22.
maternal process involved in female pregnancy A reproductive process occurring in the mother that allows an embryo or fetus to develop within it.
odontogenesis The process whose specific outcome is the progression of a tooth or teeth over time, from formation to the mature structure(s). A tooth is any hard bony, calcareous, or chitinous organ found in the mouth or pharynx of an animal and used in procuring or masticating food.
ovulation cycle process A process involved in the sexual cycle seen in females, often with physiologic changes in the endometrium that recur at regular intervals during the reproductive years.
peptide amidation The posttranslational conversion of C-terminal glycine-extended peptides to C-terminal alpha-amidated peptides. Occurs to over half of all peptide hormones to give bioactive peptides. This is a two step process catalyzed by a peptidyl-glycine alpha-hydroxylating monooxygenase and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase. In some organisms, this process is catalyzed by two separate enzymes, whereas in higher organisms, one polypeptide catalyzes both reactions.
peptide metabolic process The chemical reactions and pathways involving peptides, compounds of two or more amino acids where the alpha carboxyl group of one is bound to the alpha amino group of another.
protein amidation Addition of an amide group from a glycine to a protein amino acid.
protein metabolic process The chemical reactions and pathways involving a protein. Includes protein modification.
regulation of actin cytoskeleton organization Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins.
regulation of protein secretion Any process that modulates the frequency, rate or extent of the controlled release of a protein from a cell.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
response to copper ion Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a copper ion stimulus.
response to estradiol Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of stimulus by estradiol, a C18 steroid hormone hydroxylated at C3 and C17 that acts as a potent estrogen.
response to glucocorticoid Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucocorticoid stimulus. Glucocorticoids are hormonal C21 corticosteroids synthesized from cholesterol with the ability to bind with the cortisol receptor and trigger similar effects. Glucocorticoids act primarily on carbohydrate and protein metabolism, and have anti-inflammatory effects.
response to hypoxia Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
response to pH Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a pH stimulus. pH is a measure of the acidity or basicity of an aqueous solution.
response to xenobiotic stimulus Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organim exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
response to zinc ion Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a zinc ion stimulus.
toxin metabolic process The chemical reactions and pathways involving a toxin, a poisonous compound (typically a protein) that is produced by cells or organisms and that can cause disease when introduced into the body or tissues of an organism.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P19021 PAM Peptidyl-glycine alpha-amidating monooxygenase Homo sapiens (Human) PR
P14925 Pam Peptidylglycine alpha-amidating monooxygenase Rattus norvegicus (Rat) PR
10 20 30 40 50 60
MAGRARSRLL LLLGLLALQS SCLAFRSPLS VFKRFKETTR SFSNECLGTT RPITPIDSSD
70 80 90 100 110 120
FTLDIRMPGV TPKESDTYFC MSMRLPVDEE AFVIDFKPRA SMDTVHHMLL FGCNMPSSTG
130 140 150 160 170 180
SYWFCDEGTC TDKANILYAW ARNAPPTRLP KGVGFRVGGE TGSKYFVLQV HYGDISAFRD
190 200 210 220 230 240
NHKDCSGVSL HLTRVPQPLI AGMYLMMSVN TVIPPGEKVV NSDISCHYKM YPMHVFAYRV
250 260 270 280 290 300
HTHHLGKVVS GYRVRNGQWT LIGRQSPQLP QAFYPVEHPV DVAFGDILAA RCVFTGEGRT
310 320 330 340 350 360
EATHIGGTSS DEMCNLYIMY YMEAKHAVSF MTCTQNVAPD MFRTIPEEAN IPIPVKSDMV
370 380 390 400 410 420
MIHGHHKETE NKEKSALIQQ PKQGEEEAFE QGDFYSLLSK LLGEREDVVH VHKYNPTEKT
430 440 450 460 470 480
ESGSDLVAEI ANVVQKKDLG RSDAREGAEH EEGGNAILVR DRIHKFHRLE STLRPAESRA
490 500 510 520 530 540
LSFQQPGEGP WEPELAGDFH VEEALEWPGV YLLPGQVSGV ALDSKNNLVI FHRGDHVWDG
550 560 570 580 590 600
NSFDSKFVYQ QRGLGPIEED TILVIDPNKA EILQSSGKNL FYLPHGLSID TDGNYWVTDV
610 620 630 640 650 660
ALHQVFKLEP RSKEGPLLVL GRSMQPGSDQ NHFCQPTDVA VEPSTGAVFV SDGYCNSRIV
670 680 690 700 710 720
QFSPSGKFIT QWGEESSGSS PKPGQFSVPH SLALVPHLNQ LCVADRENGR IQCFKTDTKE
730 740 750 760 770 780
FVREIKHASF GRNVFAISYI PGFLFAVNGK PYFGDQEPVQ GFVMNFSSGE IIDVFKPVRK
790 800 810 820 830 840
HFDMPHDIVA SEDGTVYIGD AHTNTVWKFT LTESRLEVEH RSVKKAGIEV PEIKEAEAVV
850 860 870 880 890 900
EPKVKNKPTS SELQKMQEKK KLIKDPGSGV PVVLITTLLV IPVVVLLAIA MFIRWKKSRA
910 920 930 940 950 960
FGDHDRKLES SSGRVLGRLR GKGSSGLNLG NFFASRKGYS RKGFDRVSTE GSDQEKDEDD
970
GSESEEEYSA PLPTPAPSS