P14925

PR
Gene name
Pam
Protein name
Peptidylglycine alpha-amidating monooxygenase
Names
PAM
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:25508
EC number
1.14.17.3: With reduced ascorbate as one donor, and incorporation of one atom of oxygen
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

912-976 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

35 structures for P14925

Entry ID Method Resolution Chain Position Source
1OPM X-ray 210 A A 45-354 PDB
1PHM X-ray 190 A A 45-354 PDB
1SDW X-ray 185 A A 43-356 PDB
1YI9 X-ray 170 A A 47-355 PDB
1YIP X-ray 220 A A 45-355 PDB
1YJK X-ray 200 A A 50-355 PDB
1YJL X-ray 240 A A 50-355 PDB
3FVZ X-ray 235 A A 498-820 PDB
3FW0 X-ray 252 A A 498-820 PDB
3MIB X-ray 235 A A 43-356 PDB
3MIC X-ray 242 A A 43-356 PDB
3MID X-ray 306 A A 43-356 PDB
3MIE X-ray 326 A A 43-356 PDB
3MIF X-ray 200 A A 43-356 PDB
3MIG X-ray 270 A A 43-356 PDB
3MIH X-ray 274 A A 43-356 PDB
3MLJ X-ray 215 A A 43-356 PDB
3MLK X-ray 310 A A 43-356 PDB
3MLL X-ray 325 A A 43-356 PDB
3PHM X-ray 210 A A 45-354 PDB
4E4Z X-ray 198 A A 45-356 PDB
5WJA X-ray 230 A A/D 45-356 PDB
5WKW X-ray 179 A A 45-356 PDB
5WM0 X-ray 240 A A 1-976 PDB
6ALA X-ray 259 A A/C 45-356 PDB
6ALV X-ray 350 A A 45-356 PDB
6AMP X-ray 248 A A 45-356 PDB
6AN3 X-ray 205 A A 45-356 PDB
6AO6 X-ray 298 A A 45-356 PDB
6AY0 X-ray 260 A A 45-356 PDB
6NCK X-ray 270 A A 45-356 PDB
8DSJ X-ray 280 A A/K 45-354 PDB
8DSL X-ray 205 A A/B 45-354 PDB
8DSN X-ray 280 A A/K 45-354 PDB
AF-P14925-F1 Predicted AlphaFoldDB

No variants for P14925

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P14925

No associated diseases with P14925

8 regional properties for P14925

Type Name Position InterPro Accession
domain Copper type II, ascorbate-dependent monooxygenase, N-terminal 65 - 176 IPR000323
repeat NHL repeat 570 - 611 IPR001258-1
repeat NHL repeat 620 - 665 IPR001258-2
repeat NHL repeat 673 - 717 IPR001258-3
repeat NHL repeat 769 - 812 IPR001258-4
conserved_site Copper type II, ascorbate-dependent monooxygenase, histidine-cluster-2 conserved site 235 - 247 IPR014783
conserved_site Copper type II, ascorbate-dependent monooxygenase, histidine-cluster-1 conserved site 107 - 114 IPR020611
domain Copper type II ascorbate-dependent monooxygenase, C-terminal 202 - 345 IPR024548

Functions

Description
EC Number 1.14.17.3 With reduced ascorbate as one donor, and incorporation of one atom of oxygen
Subcellular Localization
  • Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein
  • Secretory granules
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

15 GO annotations of cellular component

Name Definition
cell surface The external part of the cell wall and/or plasma membrane.
chromatin The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
condensed chromosome A highly compacted molecule of DNA and associated proteins resulting in a cytologically distinct structure.
extracellular region The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
extracellular space That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid.
integral component of membrane The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
neuron projection A prolongation or process extending from a nerve cell, e.g. an axon or dendrite.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
perikaryon The portion of the cell soma (neuronal cell body) that excludes the nucleus.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
secretory granule A small subcellular vesicle, surrounded by a membrane, that is formed from the Golgi apparatus and contains a highly concentrated protein destined for secretion. Secretory granules move towards the periphery of the cell and upon stimulation, their membranes fuse with the cell membrane, and their protein load is exteriorized. Processing of the contained protein may take place in secretory granules.
secretory granule membrane The lipid bilayer surrounding a secretory granule.
trans-Golgi network The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side distal to the endoplasmic reticulum, from which secretory vesicles emerge. The trans-Golgi network is important in the later stages of protein secretion where it is thought to play a key role in the sorting and targeting of secreted proteins to the correct destination.
transport vesicle membrane The lipid bilayer surrounding a transport vesicle.

9 GO annotations of molecular function

Name Definition
calcium ion binding Binding to a calcium ion (Ca2+).
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
copper ion binding Binding to a copper (Cu) ion.
identical protein binding Binding to an identical protein or proteins.
L-ascorbic acid binding Binding to L-ascorbic acid, (2R)-2-[(1S)-1,2-dihydroxyethyl]-4-hydroxy-5-oxo-2,5-dihydrofuran-3-olate; L-ascorbic acid is vitamin C and has co-factor and anti-oxidant activities in many species.
peptidylamidoglycolate lyase activity Catalysis of the reaction: peptidylamidoglycolate = peptidyl amide + glyoxylate.
peptidylglycine monooxygenase activity Catalysis of the reaction: peptidyl-glycine + ascorbate + O2 = peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
zinc ion binding Binding to a zinc ion (Zn).

25 GO annotations of biological process

Name Definition
central nervous system development The process whose specific outcome is the progression of the central nervous system over time, from its formation to the mature structure. The central nervous system is the core nervous system that serves an integrating and coordinating function. In vertebrates it consists of the brain and spinal cord. In those invertebrates with a central nervous system it typically consists of a brain, cerebral ganglia and a nerve cord.
fatty acid primary amide biosynthetic process The chemical reactions and pathways resulting in the formation of a fatty acid primary amide.
heart development The process whose specific outcome is the progression of the heart over time, from its formation to the mature structure. The heart is a hollow, muscular organ, which, by contracting rhythmically, keeps up the circulation of the blood.
lactation The regulated release of milk from the mammary glands and the period of time that a mother lactates to feed her young.
limb development The process whose specific outcome is the progression of a limb over time, from its formation to the mature structure. A limb is an appendage of an animal used for locomotion or grasping. Examples include legs, arms or some types of fin.
long-chain fatty acid metabolic process The chemical reactions and pathways involving long-chain fatty acids, A long-chain fatty acid is a fatty acid with a chain length between C13 and C22.
maternal process involved in female pregnancy A reproductive process occurring in the mother that allows an embryo or fetus to develop within it.
mitotic chromosome condensation The cell cycle process in which chromatin structure is compacted prior to and during mitosis in eukaryotic cells.
odontogenesis The process whose specific outcome is the progression of a tooth or teeth over time, from formation to the mature structure(s). A tooth is any hard bony, calcareous, or chitinous organ found in the mouth or pharynx of an animal and used in procuring or masticating food.
ovulation cycle process A process involved in the sexual cycle seen in females, often with physiologic changes in the endometrium that recur at regular intervals during the reproductive years.
peptide amidation The posttranslational conversion of C-terminal glycine-extended peptides to C-terminal alpha-amidated peptides. Occurs to over half of all peptide hormones to give bioactive peptides. This is a two step process catalyzed by a peptidyl-glycine alpha-hydroxylating monooxygenase and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase. In some organisms, this process is catalyzed by two separate enzymes, whereas in higher organisms, one polypeptide catalyzes both reactions.
peptide metabolic process The chemical reactions and pathways involving peptides, compounds of two or more amino acids where the alpha carboxyl group of one is bound to the alpha amino group of another.
protein amidation Addition of an amide group from a glycine to a protein amino acid.
protein metabolic process The chemical reactions and pathways involving a protein. Includes protein modification.
regulation of actin cytoskeleton organization Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins.
regulation of protein secretion Any process that modulates the frequency, rate or extent of the controlled release of a protein from a cell.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
response to copper ion Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a copper ion stimulus.
response to estradiol Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of stimulus by estradiol, a C18 steroid hormone hydroxylated at C3 and C17 that acts as a potent estrogen.
response to glucocorticoid Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucocorticoid stimulus. Glucocorticoids are hormonal C21 corticosteroids synthesized from cholesterol with the ability to bind with the cortisol receptor and trigger similar effects. Glucocorticoids act primarily on carbohydrate and protein metabolism, and have anti-inflammatory effects.
response to hypoxia Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
response to pH Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a pH stimulus. pH is a measure of the acidity or basicity of an aqueous solution.
response to xenobiotic stimulus Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organim exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
response to zinc ion Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a zinc ion stimulus.
toxin metabolic process The chemical reactions and pathways involving a toxin, a poisonous compound (typically a protein) that is produced by cells or organisms and that can cause disease when introduced into the body or tissues of an organism.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P19021 PAM Peptidyl-glycine alpha-amidating monooxygenase Homo sapiens (Human) PR
P97467 Pam Peptidyl-glycine alpha-amidating monooxygenase Mus musculus (Mouse) PR
10 20 30 40 50 60
MAGRARSGLL LLLLGLLALQ SSCLAFRSPL SVFKRFKETT RSFSNECLGT IGPVTPLDAS
70 80 90 100 110 120
DFALDIRMPG VTPKESDTYF CMSMRLPVDE EAFVIDFKPR ASMDTVHHML LFGCNMPSST
130 140 150 160 170 180
GSYWFCDEGT CTDKANILYA WARNAPPTRL PKGVGFRVGG ETGSKYFVLQ VHYGDISAFR
190 200 210 220 230 240
DNHKDCSGVS VHLTRVPQPL IAGMYLMMSV DTVIPPGEKV VNADISCQYK MYPMHVFAYR
250 260 270 280 290 300
VHTHHLGKVV SGYRVRNGQW TLIGRQNPQL PQAFYPVEHP VDVTFGDILA ARCVFTGEGR
310 320 330 340 350 360
TEATHIGGTS SDEMCNLYIM YYMEAKYALS FMTCTKNVAP DMFRTIPAEA NIPIPVKPDM
370 380 390 400 410 420
VMMHGHHKEA ENKEKSALMQ QPKQGEEEVL EQGDFYSLLS KLLGEREDVH VHKYNPTEKT
430 440 450 460 470 480
ESGSDLVAEI ANVVQKKDLG RSDAREGAEH EEWGNAILVR DRIHRFHQLE STLRPAESRA
490 500 510 520 530 540
FSFQQPGEGP WEPEPSGDFH VEEELDWPGV YLLPGQVSGV ALDSKNNLVI FHRGDHVWDG
550 560 570 580 590 600
NSFDSKFVYQ QRGLGPIEED TILVIDPNNA EILQSSGKNL FYLPHGLSID TDGNYWVTDV
610 620 630 640 650 660
ALHQVFKLDP HSKEGPLLIL GRSMQPGSDQ NHFCQPTDVA VEPSTGAVFV SDGYCNSRIV
670 680 690 700 710 720
QFSPSGKFVT QWGEESSGSS PRPGQFSVPH SLALVPHLDQ LCVADRENGR IQCFKTDTKE
730 740 750 760 770 780
FVREIKHASF GRNVFAISYI PGFLFAVNGK PYFGDQEPVQ GFVMNFSSGE IIDVFKPVRK
790 800 810 820 830 840
HFDMPHDIVA SEDGTVYIGD AHTNTVWKFT LTEKMEHRSV KKAGIEVQEI KEAEAVVEPK
850 860 870 880 890 900
VENKPTSSEL QKMQEKQKLS TEPGSGVSVV LITTLLVIPV LVLLAIVMFI RWKKSRAFGD
910 920 930 940 950 960
HDRKLESSSG RVLGRFRGKG SGGLNLGNFF ASRKGYSRKG FDRVSTEGSD QEKDEDDGTE
970
SEEEYSAPLP KPAPSS