P97386

PR
Gene name
Lig3
Protein name
DNA ligase 3
Names
DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3
Species
Mus musculus (Mouse)
KEGG Pathway
EC number
6.5.1.1: Forming phosphoric ester bonds
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

169-248 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P97386

Entry ID Method Resolution Chain Position Source
AF-P97386-F1 Predicted AlphaFoldDB

No variants for P97386

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P97386

No associated diseases with P97386

8 regional properties for P97386

Type Name Position InterPro Accession
domain BRCT domain 939 - 1015 IPR001357
domain Zinc finger, PARP-type 94 - 186 IPR001510
domain DNA ligase, ATP-dependent, N-terminal 266 - 438 IPR012308
domain DNA ligase, ATP-dependent, C-terminal 710 - 819 IPR012309
domain DNA ligase, ATP-dependent, central 488 - 722 IPR012310
conserved_site DNA ligase, ATP-dependent, conserved site 511 - 519 IPR016059-1
conserved_site DNA ligase, ATP-dependent, conserved site 660 - 682 IPR016059-2
domain DNA ligase 3, BRCT domain 940 - 1009 IPR031916

Functions

Description
EC Number 6.5.1.1 Forming phosphoric ester bonds
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
condensed nuclear chromosome A highly compacted molecule of DNA and associated proteins resulting in a cytologically distinct nuclear chromosome.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
DNA ligase III-XRCC1 complex A protein complex that contains DNA ligase III and XRCC1, and is involved in base excision repair.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
synaptonemal complex A proteinaceous scaffold found between homologous chromosomes during meiosis. It consists of 2 lateral elements and a central element, all running parallel to each other. Transverse filaments connect the lateral elements to the central element.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA ligase (ATP) activity Catalysis of the reaction: ATP + deoxyribonucleotide(n) + deoxyribonucleotide(m) = AMP + diphosphate + deoxyribonucleotide(n+m).
DNA ligase activity Catalysis of the formation of a phosphodiester bond between the 3'-hydroxyl group at the end of one DNA chain and the 5'-phosphate group at the end of another. This reaction requires an energy source such as ATP or NAD+.
zinc ion binding Binding to a zinc ion (Zn).

14 GO annotations of biological process

Name Definition
base-excision repair, DNA ligation The ligation by DNA ligase of DNA strands. Ligation occurs after polymerase action to fill the gap left by the action of endonucleases during base-excision repair.
cell cycle The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division.
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
DNA biosynthetic process The biosynthetic process resulting in the formation of DNA.
DNA ligation The re-formation of a broken phosphodiester bond in the DNA backbone, carried out by DNA ligase.
DNA recombination Any process in which a new genotype is formed by reassortment of genes resulting in gene combinations different from those that were present in the parents. In eukaryotes genetic recombination can occur by chromosome assortment, intrachromosomal recombination, or nonreciprocal interchromosomal recombination. Interchromosomal recombination occurs by crossing over. In bacteria it may occur by genetic transformation, conjugation, transduction, or F-duction.
double-strand break repair The repair of double-strand breaks in DNA via homologous and nonhomologous mechanisms to reform a continuous DNA helix.
double-strand break repair via alternative nonhomologous end joining An instance of double-strand break repair via nonhomologous end joining that is independent of factors important for V(D)J recombination (as opposed to classical nonhomologous end joining). It often results in a deletion with microhomology (i.e. 5-25bp homology) at the repair junction. Among different subclasses of nonhomologous end joining (NHEJ), alternative NHEJ appears to play a significant role in the etiology of mutations that arise during cancer development and treatment.
double-strand break repair via nonhomologous end joining The repair of a double-strand break in DNA in which the two broken ends are rejoined with little or no sequence complementarity. Information at the DNA ends may be lost due to the modification of broken DNA ends. This term covers instances of separate pathways, called classical (or canonical) and alternative nonhomologous end joining (C-NHEJ and A-NHEJ). These in turn may further branch into sub-pathways, but evidence is still unclear.
lagging strand elongation The process in which an existing DNA strand is extended in a net 3' to 5' direction by activities including the addition of nucleotides to the 3' end of the strand, complementary to an existing template, as part of DNA replication. Lagging strand DNA elongation proceeds by discontinuous synthesis of short stretches of DNA, known as Okazaki fragments, from RNA primers; these fragments are then joined by DNA ligase. Although each segment of nascent DNA is synthesized in the 5' to 3' direction, the overall direction of lagging strand synthesis is 3' to 5', mirroring the progress of the replication fork.
mitochondrial DNA repair The process of restoring mitochondrial DNA after damage.
mitochondrion organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a mitochondrion; includes mitochondrial morphogenesis and distribution, and replication of the mitochondrial genome as well as synthesis of new mitochondrial components.
negative regulation of DNA recombination Any process that stops, prevents, or reduces the frequency, rate or extent of DNA recombination.
negative regulation of mitochondrial DNA replication Any process that decreases the rate, frequency or extent of the process in which new strands of DNA are synthesized in the mitochondrion.

1 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P37913 Lig1 DNA ligase 1 Mus musculus (Mouse) PR
10 20 30 40 50 60
MTLAFKILFP RNLCALGRKE LCLFPEQNRW AAISQFSQWS ETNLLGGCCL LQRRKPVLAL
70 80 90 100 110 120
QRGHLRPRAT HLTFWPGSHV GLCTGPCAMA EQRFCVDYAK RGTAGCKKCK EKIVKGVCRI
130 140 150 160 170 180
GKVVPNPFSE SGGDMKEWYH IKCMFEKLER ARATTKKIED LTELEGWEEL EDNEKEQISQ
190 200 210 220 230 240
HIADLSSKAA ATPKKKAAVQ AKLTTTGQVT SPVKGASFIT STNPRKFSGF SAAKPNNSEQ
250 260 270 280 290 300
APSSPAPGTS LSASKCDPKH KDCLLREFRK LCAMVAENPS YNTKTQIIHD FLQKGSTGGF
310 320 330 340 350 360
SDGFHGDVYL TVKLLLPGVI KSVYNLNDKQ IVKLFSRIFN CNPDDMARDL EQGDVSETIR
370 380 390 400 410 420
IFFEQSKSFP PAAKSLLTIQ EVDAFLLHLS KLTKEDEQQQ ALQDIASRCT ANDLKCIIRL
430 440 450 460 470 480
IKHDLKMNSG AKHVLDALDP NAYEAFKASR NLQDVVERVL HNEQEVEKDP GRRRALRVQA
490 500 510 520 530 540
SLMTPVQPML AEACKSIEYA MKKCPNGMFS EIKYDGERVQ VHKKGDHFSY FSRSLKPVLP
550 560 570 580 590 600
HKVAHFKDYI PKAFPGGQSM ILDSEVLLID NNTGKPLPFG TLGVHKKAAF QDANVCLFVF
610 620 630 640 650 660
DCIYFNDVSL MDRPLCERRK FLHDNMVEIR NRIMFSEMKQ VTKASDLADM INRVIREGLE
670 680 690 700 710 720
GLVLKDVKGT YEPGKRHWLK VKKDYLNEGA MADTADLVVL GAFYGQGSKG GMMSIFLMGC
730 740 750 760 770 780
YDPDSQKWCT VTKCAGGHDD ATLARLQKEL VMVKISKDPS KIPSWLKINK IYYPDFIVPD
790 800 810 820 830 840
PKKAAVWEIT GAEFSRSEAH TADGISIRFP RCTRIRDDKD WKSATNLPQL KELYQLSKDK
850 860 870 880 890 900
ADFAVVAGDE ASPTTGGSSG ENEGTAGSAG PCKGPPSKSS ASAKTTEQKL NSPSSRGGIK
910 920 930 940 950 960
PIPKHSPMKP GEKLAVKSSP VKVGMKRKAT DETPCLKKVL LDVFTGVRLY LPPSTPDFKR
970 980 990 1000 1010
LKRYFVAFDG DLVQEFDMGS ATHVLGNREK NTDAQLVSSE WIWACIRKRR LIAPC