P49960
PR
Gene name |
PRP24 (YMR268C, YM8156.10C) |
Protein name |
U4/U6 snRNA-associated-splicing factor PRP24 |
Names |
U4/U6 snRNP protein |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YMR268C |
EC number |
|
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
9 structures for P49960
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 2GHP | X-ray | 270 A | A/B/C/D/E/F/G/H | 1-291 | PDB |
| 2GO9 | NMR | - | A | 38-197 | PDB |
| 2KH9 | NMR | - | A | 115-197 | PDB |
| 2L9W | NMR | - | A | 292-400 | PDB |
| 4N0T | X-ray | 170 A | A | 34-400 | PDB |
| 5TF6 | X-ray | 230 A | A/C | 34-400 | PDB |
| 5VSU | X-ray | 310 A | A | 1-444 | PDB |
| 6ASO | X-ray | 271 A | A | 28-444 | PDB |
| AF-P49960-F1 | Predicted | AlphaFoldDB |
17 variants for P49960
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s13-804025 | 66 | P>L | No | SGRP | |
| s13-804015 | 69 | H>Q | No | SGRP | |
| s13-804005 | 73 | A>T | No | SGRP | |
| s13-803798 | 142 | V>I | No | SGRP | |
| s13-803710 | 171 | A>G | No | SGRP | |
| s13-803535 | 229 | E>D | No | SGRP | |
| s13-803536 | 229 | E>G | No | SGRP | |
| s13-803522 | 234 | F>I | No | SGRP | |
| s13-803438 | 262 | N>D | No | SGRP | |
| s13-803210 | 338 | I>V | No | SGRP | |
| s13-803183 | 347 | L>F | No | SGRP | |
| s13-803153 | 357 | I>V | No | SGRP | |
| s13-803148 | 358 | I>M | No | SGRP | |
| s13-803020 | 401 | S>N | No | SGRP | |
| s13-803012 | 404 | H>Y | No | SGRP | |
| s13-802988 | 412 | M>V | No | SGRP | |
| s13-802949 | 425 | I>F | No | SGRP |
No associated diseases with P49960
9 regional properties for P49960
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | RNA recognition motif domain | 41 - 116 | IPR000504-1 |
| domain | RNA recognition motif domain | 117 - 195 | IPR000504-2 |
| domain | RNA recognition motif domain | 210 - 289 | IPR000504-3 |
| domain | RNA recognition motif domain | 313 - 385 | IPR000504-4 |
| domain | LSM-interacting domain | 425 - 443 | IPR008669 |
| domain | Occluded RNA-recognition motif | 312 - 392 | IPR031766 |
| domain | Prp24, RNA recognition motif1 | 41 - 113 | IPR034397 |
| domain | Prp24, RNA recognition motif2 | 117 - 195 | IPR034398 |
| domain | Prp24, RNA recognition motif 3 | 210 - 286 | IPR034540 |
6 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasmic stress granule | A dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| ribonucleoprotein complex | A macromolecular complex that contains both RNA and protein molecules. |
| spliceosomal complex | Any of a series of ribonucleoprotein complexes that contain snRNA(s) and small nuclear ribonucleoproteins (snRNPs), and are formed sequentially during the spliceosomal splicing of one or more substrate RNAs, and which also contain the RNA substrate(s) from the initial target RNAs of splicing, the splicing intermediate RNA(s), to the final RNA products. During cis-splicing, the initial target RNA is a single, contiguous RNA transcript, whether mRNA, snoRNA, etc., and the released products are a spliced RNA and an excised intron, generally as a lariat structure. During trans-splicing, there are two initial substrate RNAs, the spliced leader RNA and a pre-mRNA. |
| U6 snRNP | A ribonucleoprotein complex that contains small nuclear RNA U6, the Lsm2-8 heptameric ring complex, as well as several proteins that are unique to the U6 snRNP, most of which remain associated with the U6 snRNA both while the U6 snRNP is free or assembled into the U4/U6 snRNP or into a series of spliceosomal complexes. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| mRNA 3'-UTR binding | Binding to a 3' untranslated region of an mRNA molecule. |
| poly(A) binding | Binding to a sequence of adenylyl residues in an RNA molecule, such as the poly(A) tail, a sequence of adenylyl residues at the 3' end of eukaryotic mRNA. |
| poly(U) RNA binding | Binding to a sequence of uracil residues in an RNA molecule. |
| RNA binding | Binding to an RNA molecule or a portion thereof. |
| snRNA binding | Binding to a small nuclear RNA (snRNA). |
| U6 snRNA binding | Binding to a U6 small nuclear RNA (U6 snRNA). |
3 GO annotations of biological process
| Name | Definition |
|---|---|
| mRNA splicing, via spliceosome | The joining together of exons from one or more primary transcripts of messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced. |
| spliceosomal complex assembly | The aggregation, arrangement and bonding together of a spliceosomal complex, a ribonucleoprotein apparatus that catalyzes nuclear mRNA splicing via transesterification reactions. |
| spliceosomal tri-snRNP complex assembly | The formation of a tri-snRNP complex containing U4 and U6 (or U4atac and U6atac) snRNAs and U5 snRNAs and associated proteins. This includes reannealing of U4 and U6 (or U4atac and U6atac) snRNAs released from previous rounds of splicing to reform the U4/U6 snRNP (or U4atac/U6atac snRNP) as well as the subsequent association of the U5 snRNP with the U4/U6 snRNP (or U4atac/U6atac snRNP) to form a tri-snRNP that is ready to reassemble into another spliceosome complex. |
1 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P38760 | MIP6 | RNA-binding protein MIP6 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MEYGHHARPD | SKRPLDEGSP | AAAGLTSKKA | NEALTRNREL | TTVLVKNLPK | SYNQNKVYKY |
| 70 | 80 | 90 | 100 | 110 | 120 |
| FKHCGPIIHV | DVADSLKKNF | RFARIEFARY | DGALAAITKT | HKVVGQNEII | VSHLTECTLW |
| 130 | 140 | 150 | 160 | 170 | 180 |
| MTNFPPSYTQ | RNIRDLLQDI | NVVALSIRLP | SLRFNTSRRF | AYIDVTSKED | ARYCVEKLNG |
| 190 | 200 | 210 | 220 | 230 | 240 |
| LKIEGYTLVT | KVSNPLEKSK | RTDSATLEGR | EIMIRNLSTE | LLDENLLRES | FEGFGSIEKI |
| 250 | 260 | 270 | 280 | 290 | 300 |
| NIPAGQKEHS | FNNCCAFMVF | ENKDSAERAL | QMNRSLLGNR | EISVSLADKK | PFLERNEVKR |
| 310 | 320 | 330 | 340 | 350 | 360 |
| LLASRNSKEL | ETLICLFPLS | DKVSPSLICQ | FLQEEIHINE | KDIRKILLVS | DFNGAIIIFR |
| 370 | 380 | 390 | 400 | 410 | 420 |
| DSKFAAKMLM | ILNGSQFQGK | VIRSGTINDM | KRYYNNQQNH | SMKHVKPSCI | NMMEKGPNLQ |
| 430 | 440 | ||||
| VKKKIPDKQE | QMSNDDFRKM | FLGE |