P49954

PR
Gene name
NIT3 (YLR351C, L9638.5)
Protein name
Omega-amidase NIT3
Names
Nitrilase homolog 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YLR351C
EC number
3.5.1.3: In linear amides
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

40-109 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for P49954

Entry ID Method Resolution Chain Position Source
1F89 X-ray 240 A A/B 1-291 PDB
AF-P49954-F1 Predicted AlphaFoldDB

4 variants for P49954

Variant ID(s) Position Change Description Diseaes Association Provenance
s12-830009 119 D>N No SGRP
s12-829992 124 D>E No SGRP
s12-829916 150 P>A No SGRP
s12-829897 156 T>I No SGRP

No associated diseases with P49954

3 regional properties for P49954

Type Name Position InterPro Accession
conserved_site Uncharacterised protein family UPF0012, conserved site 165 - 185 IPR001110
domain Carbon-nitrogen hydrolase 11 - 272 IPR003010
domain Nit1/2, carbon-nitrogen hydrolase domain 12 - 281 IPR045254

Functions

Description
EC Number 3.5.1.3 In linear amides
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

2 GO annotations of molecular function

Name Definition
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Catalysis of the hydrolysis of any non-peptide carbon-nitrogen bond in a linear amide.
omega-amidase activity Catalysis of the reaction: a monoamide of a dicarboxylic acid + H2O = a dicarboxylate + NH3.

4 GO annotations of biological process

Name Definition
asparagine metabolic process The chemical reactions and pathways involving asparagine, 2-amino-3-carbamoylpropanoic acid.
cellular amide catabolic process The chemical reactions and pathways resulting in the breakdown of an amide, any derivative of an oxoacid in which an acidic hydroxy group has been replaced by an amino or substituted amino group.
glutamine metabolic process The chemical reactions and pathways involving glutamine, 2-amino-4-carbamoylbutanoic acid.
oxaloacetate metabolic process The chemical reactions and pathways involving oxaloacetate, the anion of oxobutanedioic acid, an important intermediate in metabolism, especially as a component of the TCA cycle.

1 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q28IE5 nit2 Omega-amidase NIT2 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) PR
10 20 30 40 50 60
MSASKILSQK IKVALVQLSG SSPDKMANLQ RAATFIERAM KEQPDTKLVV LPECFNSPYS
70 80 90 100 110 120
TDQFRKYSEV INPKEPSTSV QFLSNLANKF KIILVGGTIP ELDPKTDKIY NTSIIFNEDG
130 140 150 160 170 180
KLIDKHRKVH LFDVDIPNGI SFHESETLSP GEKSTTIDTK YGKFGVGICY DMRFPELAML
190 200 210 220 230 240
SARKGAFAMI YPSAFNTVTG PLHWHLLARS RAVDNQVYVM LCSPARNLQS SYHAYGHSIV
250 260 270 280 290
VDPRGKIVAE AGEGEEIIYA ELDPEVIESF RQAVPLTKQR RFDVYSDVNA H