P32356

PR
Gene name
NTH1
Protein name
Cytosolic neutral trehalase
Names
Alpha,alpha-trehalase, Alpha,alpha-trehalose glucohydrolase
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YDR001C
EC number
3.2.1.28: Glycosidases, ie enzymes hydrolyzing O- and S-glycosyl compounds
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-50 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

5 structures for P32356

Entry ID Method Resolution Chain Position Source
5JTA X-ray 272 A A 1-751 PDB
5M4A X-ray 290 A A 153-751 PDB
5N6N X-ray 229 A C 1-751 PDB
5NIS X-ray 315 A A 100-751 PDB
AF-P32356-F1 Predicted AlphaFoldDB

7 variants for P32356

Variant ID(s) Position Change Description Diseaes Association Provenance
s04-452438 12 A>G No SGRP
s04-451796 226 Y>F No SGRP
s04-451347 376 S>G No SGRP
s04-451260 405 L>F No SGRP
s04-451142 444 T>S No SGRP
s04-450954 507 A>S No SGRP
s04-450561 638 W>G No SGRP

No associated diseases with P32356

3 regional properties for P32356

Type Name Position InterPro Accession
domain Neutral trehalase Ca2+ binding 106 - 135 IPR011120
conserved_site Glycoside hydrolase, family 37, conserved site 299 - 312 IPR018232-1
conserved_site Glycoside hydrolase, family 37, conserved site 623 - 632 IPR018232-2

Functions

Description
EC Number 3.2.1.28 Glycosidases, ie enzymes hydrolyzing O- and S-glycosyl compounds
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

3 GO annotations of molecular function

Name Definition
alpha,alpha-trehalase activity Catalysis of the reaction: alpha,alpha-trehalose + H2O = 2 D-glucose.
calcium ion binding Binding to a calcium ion (Ca2+).
trehalase activity Catalysis of the hydrolysis of trehalose or a trehalose derivative.

2 GO annotations of biological process

Name Definition
cellular response to desiccation Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a desiccation stimulus, extreme dryness resulting from the prolonged deprivation of water.
trehalose catabolic process The chemical reactions and pathways resulting in the breakdown of trehalose, a disaccharide isomeric with sucrose and obtained from certain lichens and fungi.

1 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P35172 NTH2 Probable trehalase Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
10 20 30 40 50 60
MSQVNTSQGP VAQGRQRRLS SLSEFNDPFS NAEVYYGPPT DPRKQKQAKP AKINRTRTMS
70 80 90 100 110 120
VFDNVSPFKK TGFGKLQQTR RGSEDDTYSS SQGNRRFFIE DVDKTLNELL AAEDTDKNYQ
130 140 150 160 170 180
ITIEDTGPKV LKVGTANSYG YKHINIRGTY MLSNLLQELT IAKSFGRHQI FLDEARINEN
190 200 210 220 230 240
PVNRLSRLIN TQFWNSLTRR VDLNNVGEIA KDTKIDTPGA KNPRIYVPYD CPEQYEFYVQ
250 260 270 280 290 300
ASQMHPSLKL EVEYLPKKIT AEYVKSVNDT PGLLALAMEE HFNPSTGEKT LIGYPYAVPG
310 320 330 340 350 360
GRFNELYGWD SYMMALGLLE ANKTDVARGM VEHFIFEINH YGKILNANRS YYLCRSQPPF
370 380 390 400 410 420
LTEMALVVFK KLGGRSNPDA VDLLKRAFQA SIKEYKTVWT ASPRLDPETG LSRYHPNGLG
430 440 450 460 470 480
IPPETESDHF DTVLLPYASK HGVTLDEFKQ LYNDGKIKEP KLDEFFLHDR GVRESGHDTT
490 500 510 520 530 540
YRFEGVCAYL ATIDLNSLLY KYEIDIADFI KEFCDDKYED PLDHSITTSA MWKEMAKIRQ
550 560 570 580 590 600
EKITKYMWDD ESGFFFDYNT KIKHRTSYES ATTFWALWAG LATKEQAQKM VEKALPKLEM
610 620 630 640 650 660
LGGLAACTER SRGPISISRP IRQWDYPFGW APHQILAWEG LRSYGYLTVT NRLAYRWLFM
670 680 690 700 710 720
MTKAFVDYNG IVVEKYDVTR GTDPHRVEAE YGNQGADFKG AATEGFGWVN ASYILGLKYM
730 740 750
NSHARRALGA CIPPISFFSS LRPQERNLYG L