P22336
PR
Gene name |
RFA1 (BUF2, RPA1, YAR007C, FUN3) |
Protein name |
Replication factor A protein 1 |
Names |
RF-A protein 1, DNA-binding protein BUF2, Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YAR007C |
EC number |
|
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
8 structures for P22336
4 variants for P22336
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s01-158599 | 8 | R>K | No | SGRP | |
| s01-158143 | 160 | S>L | No | SGRP | |
| s01-157674 | 316 | N>K | No | SGRP | |
| s01-157271 | 451 | A>T | No | SGRP |
No associated diseases with P22336
5 regional properties for P22336
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | OB-fold nucleic acid binding domain, AA-tRNA synthetase-type | 197 - 280 | IPR004365 |
| domain | Replication factor-A protein 1, N-terminal | 9 - 107 | IPR007199 |
| domain | Replication factor A, C-terminal | 466 - 611 | IPR013955 |
| domain | Replication protein A, OB domain | 306 - 400 | IPR031657 |
| domain | Replication factor A, C-terminal, eukaryotes | 450 - 617 | IPR047192 |
6 GO annotations of cellular component
| Name | Definition |
|---|---|
| chromosome, telomeric region | The end of a linear chromosome, required for the integrity and maintenance of the end. A chromosome telomere usually includes a region of telomerase-encoded repeats the length of which rarely exceeds 20 bp each and that permits the formation of a telomeric loop (T-loop). The telomeric repeat region is usually preceded by a sub-telomeric region that is gene-poor but rich in repetitive elements. Some telomeres only consist of the latter part (for eg. D. melanogaster telomeres). |
| condensed nuclear chromosome | A highly compacted molecule of DNA and associated proteins resulting in a cytologically distinct nuclear chromosome. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| DNA replication factor A complex | A conserved heterotrimeric complex that binds nonspecifically to single-stranded DNA and is required for multiple processes in eukaryotic DNA metabolism, including DNA replication, DNA repair, and recombination. In all eukaryotic organisms examined the complex is composed of subunits of approximately 70, 30, and 14 kDa. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
7 GO annotations of molecular function
| Name | Definition |
|---|---|
| damaged DNA binding | Binding to damaged DNA. |
| double-stranded DNA binding | Binding to double-stranded DNA. |
| metal ion binding | Binding to a metal ion. |
| mRNA binding | Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns. |
| sequence-specific DNA binding | Binding to DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding. |
| single-stranded DNA binding | Binding to single-stranded DNA. |
| single-stranded telomeric DNA binding | Binding to single-stranded telomere-associated DNA. |
17 GO annotations of biological process
| Name | Definition |
|---|---|
| DNA repair | The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway. |
| DNA replication | The cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by initiation proteins, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA. |
| DNA topological change | The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number. |
| DNA unwinding involved in DNA replication | The process in which interchain hydrogen bonds between two strands of DNA are broken or 'melted', generating unpaired template strands for DNA replication. |
| double-strand break repair via homologous recombination | The error-free repair of a double-strand break in DNA in which the broken DNA molecule is repaired using homologous sequences. A strand in the broken DNA searches for a homologous region in an intact chromosome to serve as the template for DNA synthesis. The restoration of two intact DNA molecules results in the exchange, reciprocal or nonreciprocal, of genetic material between the intact DNA molecule and the broken DNA molecule. |
| establishment of protein localization | The directed movement of a protein to a specific location. |
| heteroduplex formation | The formation of a stable duplex DNA that contains one strand from each of the two recombining DNA molecules. |
| meiotic cell cycle | Progression through the phases of the meiotic cell cycle, in which canonically a cell replicates to produce four offspring with half the chromosomal content of the progenitor cell via two nuclear divisions. |
| mitotic recombination | The exchange, reciprocal or nonreciprocal, of genetic material between one DNA molecule and a homologous DNA region that occurs during mitotic cell cycles. |
| nucleotide-excision repair | A DNA repair process in which a small region of the strand surrounding the damage is removed from the DNA helix as an oligonucleotide. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. Nucleotide excision repair recognizes a wide range of substrates, including damage caused by UV irradiation (pyrimidine dimers and 6-4 photoproducts) and chemicals (intrastrand cross-links and bulky adducts). |
| protein ubiquitination | The process in which one or more ubiquitin groups are added to a protein. |
| reciprocal meiotic recombination | The cell cycle process in which double strand breaks are formed and repaired through a single or double Holliday junction intermediate. This results in the equal exchange of genetic material between non-sister chromatids in a pair of homologous chromosomes. These reciprocal recombinant products ensure the proper segregation of homologous chromosomes during meiosis I and create genetic diversity. |
| sporulation | The process whose specific outcome is the progression of a spore over time, from its initiation to the mature structure. A spore is a structure that can be used for dissemination, for survival of adverse conditions because of its heat and dessication resistance, and/or for reproduction. |
| telomere maintenance | Any process that contributes to the maintenance of proper telomeric length and structure by affecting and monitoring the activity of telomeric proteins, the length of telomeric DNA and the replication and repair of the DNA. These processes includes those that shorten, lengthen, replicate and repair the telomeric DNA sequences. |
| telomere maintenance via recombination | Any recombinational process that contributes to the maintenance of proper telomeric length. |
| telomere maintenance via telomerase | The maintenance of proper telomeric length by the addition of telomeric repeats by telomerase. |
| telomere maintenance via telomere lengthening | Any process that contributes to the maintenance of proper telomeric length and structure by affecting and monitoring the activity of telomeric proteins and lengthening the telomeric DNA. |
5 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q5ZJJ2 | RPA1 | Replication protein A 70 kDa DNA-binding subunit | Gallus gallus (Chicken) | PR |
| Q24492 | RpA-70 | Replication protein A 70 kDa DNA-binding subunit | Drosophila melanogaster (Fruit fly) | PR |
| P27694 | RPA1 | Replication protein A 70 kDa DNA-binding subunit | Homo sapiens (Human) | PR |
| Q8VEE4 | Rpa1 | Replication protein A 70 kDa DNA-binding subunit | Mus musculus (Mouse) | PR |
| Q5FW17 | rpa1 | Replication protein A 70 kDa DNA-binding subunit | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSSVQLSRGD | FHSIFTNKQR | YDNPTGGVYQ | VYNTRKSDGA | NSNRKNLIMI | SDGIYHMKAL |
| 70 | 80 | 90 | 100 | 110 | 120 |
| LRNQAASKFQ | SMELQRGDII | RVIIAEPAIV | RERKKYVLLV | DDFELVQSRA | DMVNQTSTFL |
| 130 | 140 | 150 | 160 | 170 | 180 |
| DNYFSEHPNE | TLKDEDITDS | GNVANQTNAS | NAGVPDMLHS | NSNLNANERK | FANENPNSQK |
| 190 | 200 | 210 | 220 | 230 | 240 |
| TRPIFAIEQL | SPYQNVWTIK | ARVSYKGEIK | TWHNQRGDGK | LFNVNFLDTS | GEIRATAFND |
| 250 | 260 | 270 | 280 | 290 | 300 |
| FATKFNEILQ | EGKVYYVSKA | KLQPAKPQFT | NLTHPYELNL | DRDTVIEECF | DESNVPKTHF |
| 310 | 320 | 330 | 340 | 350 | 360 |
| NFIKLDAIQN | QEVNSNVDVL | GIIQTINPHF | ELTSRAGKKF | DRRDITIVDD | SGFSISVGLW |
| 370 | 380 | 390 | 400 | 410 | 420 |
| NQQALDFNLP | EGSVAAIKGV | RVTDFGGKSL | SMGFSSTLIP | NPEIPEAYAL | KGWYDSKGRN |
| 430 | 440 | 450 | 460 | 470 | 480 |
| ANFITLKQEP | GMGGQSAASL | TKFIAQRITI | ARAQAENLGR | SEKGDFFSVK | AAISFLKVDN |
| 490 | 500 | 510 | 520 | 530 | 540 |
| FAYPACSNEN | CNKKVLEQPD | GTWRCEKCDT | NNARPNWRYI | LTISIIDETN | QLWLTLFDDQ |
| 550 | 560 | 570 | 580 | 590 | 600 |
| AKQLLGVDAN | TLMSLKEEDP | NEFTKITQSI | QMNEYDFRIR | AREDTYNDQS | RIRYTVANLH |
| 610 | 620 | ||||
| SLNYRAEADY | LADELSKALL | A |