P21499

PR
Gene name
rnr (vacB, yjeC, b4179, JW5741)
Protein name
Ribonuclease R
Names
RNase R, Protein VacB
Species
Escherichia coli (strain K12)
KEGG Pathway
eco:b4179
EC number
3.1.13.1: Exoribonucleases producing 5'-phosphomonoesters
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-73 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P21499

Entry ID Method Resolution Chain Position Source
AF-P21499-F1 Predicted AlphaFoldDB

No variants for P21499

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P21499

No associated diseases with P21499

8 regional properties for P21499

Type Name Position InterPro Accession
domain Ribonuclease II/R 260 - 588 IPR001900
domain S1 domain 641 - 725 IPR003029
domain Cold shock domain 86 - 145 IPR011129
domain Ribonuclease B, N-terminal OB domain 87 - 144 IPR013223
domain Ribonuclease R winged-helix domain 23 - 83 IPR013668
conserved_site Ribonuclease II/R, conserved site 555 - 579 IPR022966
domain RNA-binding domain, S1 642 - 725 IPR022967
domain RNase II/RNase R, cold shock domain 164 - 238 IPR040476

Functions

Description
EC Number 3.1.13.1 Exoribonucleases producing 5'-phosphomonoesters
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

7 GO annotations of molecular function

Name Definition
3'-5' RNA helicase activity Unwinding of an RNA helix in the 3' to 5' direction, driven by ATP hydrolysis.
3'-5'-exoribonuclease activity Catalysis of the sequential cleavage of mononucleotides from a free 3' terminus of an RNA molecule.
exoribonuclease activity, producing 5'-phosphomonoesters Catalysis of the hydrolysis of ester linkages within ribonucleic acids by removing nucleotide residues from the 3' or 5' end to yield 5' phosphomonoesters.
exoribonuclease II activity Catalysis of the reaction: RNA + H2O = 5'-phosphomononucleotides. Cleaves RNA in the 3' to 5' direction.
ribonuclease activity Catalysis of the hydrolysis of phosphodiester bonds in chains of RNA.
ribonuclease R activity Catalysis of the reaction: RNA + H2O = 5'-phosphomononucleotides. Cleaves RNA in the 3' to 5' direction, leaving an undigested core of 3-5 nucleotides.
RNA binding Binding to an RNA molecule or a portion thereof.

3 GO annotations of biological process

Name Definition
mRNA catabolic process The chemical reactions and pathways resulting in the breakdown of mRNA, messenger RNA, which is responsible for carrying the coded genetic 'message', transcribed from DNA, to sites of protein assembly at the ribosomes.
ncRNA processing Any process that results in the conversion of one or more primary non-coding RNA (ncRNA) transcripts into one or more mature ncRNA molecules.
response to cold Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cold stimulus, a temperature stimulus below the optimal temperature for that organism.

1 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q0P4R5 dis3l DIS3-like exonuclease 1 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) PR
10 20 30 40 50 60
MSQDPFQERE AEKYANPIPS REFILEHLTK REKPASRDEL AVELHIEGEE QLEGLRRRLR
70 80 90 100 110 120
AMERDGQLVF TRRQCYALPE RLDLVKGTVI GHRDGYGFLR VEGRKDDLYL SSEQMKTCIH
130 140 150 160 170 180
GDQVLAQPLG ADRKGRREAR IVRVLVPKTS QIVGRYFTEA GVGFVVPDDS RLSFDILIPP
190 200 210 220 230 240
DQIMGARMGF VVVVELTQRP TRRTKAVGKI VEVLGDNMGT GMAVDIALRT HEIPYIWPQA
250 260 270 280 290 300
VEQQVAGLKE EVPEEAKAGR VDLRDLPLVT IDGEDARDFD DAVYCEKKRG GGWRLWVAIA
310 320 330 340 350 360
DVSYYVRPST PLDREARNRG TSVYFPSQVI PMLPEVLSNG LCSLNPQVDR LCMVCEMTVS
370 380 390 400 410 420
SKGRLTGYKF YEAVMSSHAR LTYTKVWHIL QGDQDLREQY APLVKHLEEL HNLYKVLDKA
430 440 450 460 470 480
REERGGISFE SEEAKFIFNA ERRIERIEQT QRNDAHKLIE ECMILANISA ARFVEKAKEP
490 500 510 520 530 540
ALFRIHDKPS TEAITSFRSV LAELGLELPG GNKPEPRDYA ELLESVADRP DAEMLQTMLL
550 560 570 580 590 600
RSMKQAIYDP ENRGHFGLAL QSYAHFTSPI RRYPDLTLHR AIKYLLAKEQ GHQGNTTETG
610 620 630 640 650 660
GYHYSMEEML QLGQHCSMAE RRADEATRDV ADWLKCDFML DQVGNVFKGV ISSVTGFGFF
670 680 690 700 710 720
VRLDDLFIDG LVHVSSLDND YYRFDQVGQR LMGESSGQTY RLGDRVEVRV EAVNMDERKI
730 740 750 760 770 780
DFSLISSERA PRNVGKTARE KAKKGDAGKK GGKRRQVGKK VNFEPDSAFR GEKKTKPKAA
790 800 810
KKDARKAKKP SAKTQKIAAA TKAKRAAKKK VAE