AiPD: Autoinhibited Protein Database

P20226

Gene name	TBP (GTF2D1, TF2D, TFIID)
Protein name	TATA-box-binding protein
Names	TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit
Species	Mus musculus (Mouse)
KEGG Pathway	hsa:6908
EC number	2.3.2.27: Aminoacyltransferases
Protein Class	UBIQUITIN LIGASE E3 ALPHA-RELATED (PTHR21497)

Descriptions

In Saccharomyces cerevisiae, RING domain-containing Ub ligase UBR1 recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Binding of dipeptides with destabilizing N-terminal residues to two substrate-binding sites of UBR1 causes dissociation of the C-terminal autoinhibitory domain of UBR1 from its N-terminal region that contains all three substrate-binding sites. This dissociation allows the interaction between UBR1 and CUP9, a transcriptional repressor of the peptide transporter PTR2, thereby accelerating the UBR1-dependent degradation of CUP9 and increasing the cell's capacity to import peptides. An aspect of autoinhibition characteristic of yeast UBR1 also is observed with mouse UBR1.

Autoinhibitory domains (AIDs)

Target domain	159-338 (TATA binding domain)
Relief mechanism	Partner binding, Others
Assay	Mutagenesis experiment, Structural analysis

AID

67-71 (Conserved interface region)

Target domain

159-338 (TATA binding domain)

Relief mechanism

Partner binding (Interaction with TFIIa, TFIIB), Others (Bluky charged arginine substitutions)

Assay

Mutagenesis experiment, Structural analysis

Accessory elements

No accessory elements

References

Chasman DI et al. (1993) "Crystal structure of yeast TATA-binding protein and model for interaction with DNA", Proceedings of the National Academy of Sciences of the United States of America, 90, 8174-8

Jackson-Fisher AJ et al. (1999) "A role for TBP dimerization in preventing unregulated gene expression", Molecular cell, 3, 717-27

Chitikila C et al. (2002) "Interplay of TBP inhibitors in global transcriptional control", Molecular cell, 10, 871-82

Autoinhibited structure

Activated structure

66 structures for P20226

Entry ID	Method	Resolution	Chain	Position	Source
1C9B	X-ray	265 A	B/F/J/N/R	159-337	PDB
1CDW	X-ray	190 A	A	159-337	PDB
1JFI	X-ray	262 A	C	159-339	PDB
1NVP	X-ray	210 A	A	159-339	PDB
1TGH	X-ray	290 A	A	156-339	PDB
4ROC	X-ray	190 A	B	159-339	PDB
4ROD	X-ray	270 A	B	159-339	PDB
4ROE	X-ray	220 A	B	159-339	PDB
5FUR	EM	850 A	A	1-339	PDB
5IY6	EM	720 A	P	1-339	PDB
5IY7	EM	860 A	P	1-339	PDB
5IY8	EM	790 A	P	1-339	PDB
5IY9	EM	630 A	P	1-339	PDB
5IYA	EM	540 A	P	1-339	PDB
5IYB	EM	390 A	P	1-339	PDB
5IYC	EM	390 A	P	1-339	PDB
5IYD	EM	390 A	P	1-339	PDB
5N9G	X-ray	270 A	B/G	159-339	PDB
6MZD	EM	980 A	T	1-339	PDB
6MZL	EM	2300 A	T	1-339	PDB
6MZM	EM	750 A	T	1-339	PDB
6O9L	EM	720 A	P	1-339	PDB
7EDX	EM	450 A	P	1-339	PDB
7EG7	EM	620 A	P	1-339	PDB
7EG8	EM	740 A	P	1-339	PDB
7EG9	EM	370 A	P	1-339	PDB
7EGA	EM	410 A	P	1-339	PDB
7EGB	EM	330 A	P	1-339	PDB
7EGC	EM	390 A	P	1-339	PDB
7EGD	EM	675 A	P	1-339	PDB
7EGE	EM	900 A	P	1-339	PDB
7EGF	EM	316 A	P	1-339	PDB
7EGI	EM	982 A	P	1-339	PDB
7EGJ	EM	864 A	P	1-339	PDB
7ENA	EM	407 A	DP	1-339	PDB
7ENC	EM	413 A	DP	1-339	PDB
7LBM	EM	480 A	P	1-339	PDB
7NVR	EM	450 A	O	1-339	PDB
7NVS	EM	280 A	O	1-339	PDB
7NVT	EM	290 A	O	1-339	PDB
7NVU	EM	250 A	O	1-339	PDB
7NVY	EM	730 A	O	1-339	PDB
7NVZ	EM	720 A	O	1-339	PDB
7NW0	EM	660 A	O	1-339	PDB
7ZWC	EM	320 A	O	1-339	PDB
7ZWD	EM	300 A	O	1-339	PDB
7ZX7	EM	340 A	O	1-339	PDB
7ZX8	EM	300 A	O	1-339	PDB
7ZXE	EM	350 A	O	1-339	PDB
8BVW	EM	400 A	O	1-339	PDB
8BYQ	EM	410 A	O	1-339	PDB
8BZ1	EM	380 A	O	1-339	PDB
8GXQ	EM	504 A	DP	1-339	PDB
8GXS	EM	416 A	DP	1-339	PDB
8ITY	EM	390 A	U	1-339	PDB
8IUE	EM	410 A	U	1-339	PDB
8IUH	EM	340 A	U	1-339	PDB
8WAK	EM	547 A	P	1-339	PDB
8WAL	EM	852 A	P	1-339	PDB
8WAN	EM	607 A	P	1-339	PDB
8WAO	EM	640 A	P	1-339	PDB
8WAP	EM	585 A	P	1-339	PDB
8WAQ	EM	629 A	P	1-339	PDB
8WAR	EM	720 A	P	1-339	PDB
8WAS	EM	613 A	P	1-339	PDB
AF-P20226-F1	Predicted				AlphaFoldDB

167 variants for P20226

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
RCV000625151 rs71815788 RCV001683617	95	Q>missing	Spinocerebellar ataxia type 17 [ClinVar]	Yes	ClinVar dbSNP
rs142540266 CA4108372 RCV001336551	137	P>L	Spinocerebellar ataxia type 17 [ClinVar]	Yes	ClinGen ClinVar ESP ExAC TOPMed dbSNP gnomAD
rs751702127 CA4108223	2	D>E		No	ClinGen ExAC gnomAD
CA4108224 rs757450205	5	N>D		No	ClinGen ExAC gnomAD
rs766191843 CA4108225	6	S>R		No	ClinGen ExAC gnomAD
CA152221959 rs1009942407	8	P>L		No	ClinGen TOPMed
CA4108227 rs764142412	11	A>T		No	ClinGen ExAC TOPMed gnomAD
CA152221976 rs949315152	17	P>A		No	ClinGen TOPMed
rs1390001535 CA366516910	19	G>V		No	ClinGen gnomAD
rs758309293 CA4108249	20	A>V		No	ClinGen ExAC gnomAD
CA152223761 rs970666552	24	G>R		No	ClinGen TOPMed gnomAD
CA4108251 rs558863660	26	P>A		No	ClinGen 1000Genomes ExAC gnomAD
rs150312586 CA4108253	27	I>F		No	ClinGen ESP ExAC TOPMed gnomAD
rs150312586 CA4108252	27	I>V		No	ClinGen ESP ExAC TOPMed gnomAD
CA4108255 rs577070091	31	M>I		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs746303115 CA4108254	31	M>V		No	ClinGen ExAC TOPMed gnomAD
rs776116052 CA4108256	32	M>R		No	ClinGen ExAC
CA366517007 rs1394976596	34	Y>F		No	ClinGen TOPMed
CA4108258 rs749788903	35	G>V		No	ClinGen ExAC gnomAD
CA366517032 rs1583127364	39	T>P		No	ClinGen Ensembl
rs375575792 CA4108260	40	P>A		No	ClinGen ESP ExAC gnomAD
CA4108261 rs761867883	44	Q>P		No	ClinGen ExAC gnomAD
CA4108262 rs772344160	47	N>S		No	ClinGen ExAC TOPMed gnomAD
CA366517111 rs773389193	51	I>L		No	ClinGen ExAC TOPMed gnomAD
rs773389193 CA4108263	51	I>V		No	ClinGen ExAC TOPMed gnomAD
CA366517132 rs1190072930	54	E>K		No	ClinGen TOPMed gnomAD
rs764966099 CA4108269	58	Q>L		No	ClinGen ExAC TOPMed gnomAD
rs764966099 CA366517167	58	Q>R		No	ClinGen ExAC TOPMed gnomAD
rs752498284 CA4108273	60	Q>H		No	ClinGen ExAC TOPMed
CA152223829 rs559386285	62	Q>H		No	ClinGen 1000Genomes TOPMed
CA366517202 rs1215217094	63	Q>*		No	ClinGen gnomAD
rs762804204 CA4108280	67	Q>R		No	ClinGen ExAC TOPMed gnomAD
rs1331989064 CA366517266	71	Q>P		No	ClinGen gnomAD
rs55736770 CA152223968	72	Q>H		No	ClinGen TOPMed
rs1410409146 CA366517279	73	Q>E		No	ClinGen gnomAD
rs764035011 CA366517284	73	Q>H		No	ClinGen ExAC TOPMed
CA366517285 rs764035011	73	Q>H		No	ClinGen ExAC TOPMed
CA366517282 rs1229412131	73	Q>R		No	ClinGen TOPMed
rs62430309 CA152223976	74	Q>H		No	ClinGen TOPMed
rs56241301 CA152223998	75	Q>H		No	ClinGen ExAC TOPMed
rs1197414368 CA366517298	75	Q>L		No	ClinGen TOPMed
CA366517306 rs112083427	76	Q>H		No	ClinGen ExAC TOPMed gnomAD
rs1336548568 CA366517303	76	Q>R		No	ClinGen TOPMed
RCV001692125 RCV000455402 rs1491487452	77	Q>missing		No	ClinVar dbSNP
RCV001725920 rs771725566 RCV001725919	77	Q>missing		No	ClinVar dbSNP
CA366517310 rs1346721481	77	Q>*		No	ClinGen gnomAD
CA366517308 rs1346721481	77	Q>K		No	ClinGen gnomAD
rs1198340310 CA366517318	78	Q>*		No	ClinGen gnomAD
rs113440919 CA4108319	78	Q>H		No	ClinGen ExAC TOPMed gnomAD
rs1198340310 CA366517316	78	Q>K		No	ClinGen gnomAD
CA4108320 rs780283741	79	Q>R		No	ClinGen ExAC
CA366517332 rs1485539992	80	Q>*		No	ClinGen gnomAD
CA366517333 rs1309337484	80	Q>R		No	ClinGen gnomAD
rs779471026 CA366517345	81	Q>H		No	ClinGen ExAC TOPMed gnomAD
rs1471864851 CA366517346	82	Q>*		No	ClinGen gnomAD
rs748266569 CA4108325	82	Q>H		No	ClinGen ExAC TOPMed gnomAD
CA366517354 rs1378238070	83	Q>*		No	ClinGen gnomAD
CA366517373 rs1401305444	85	Q>R		No	ClinGen gnomAD
CA366517379 rs1236899437	86	Q>*		No	ClinGen gnomAD
CA366517390 rs1309553160	87	Q>R		No	ClinGen gnomAD
CA366517398 rs1562359677	88	Q>R		No	ClinGen Ensembl
CA4108333 rs764009292	89	Q>R		No	ClinGen ExAC TOPMed
CA152224089 rs982117967	90	Q>R		No	ClinGen TOPMed
CA152224114 rs796895015	92	Q>H		No	ClinGen TOPMed
CA152224109 rs865926586	92	Q>R		No	ClinGen Ensembl
rs1156940638 CA366517433	93	Q>R		No	ClinGen TOPMed
RCV000736062 rs752404282	93	Q>missing		No	ClinVar dbSNP
CA366517438 rs1273872785	94	Q>E		No	ClinGen TOPMed gnomAD
rs143655307 CA4108344	95	Q>H		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs752404282 RCV000736063	95	Q>missing		No	ClinVar dbSNP
rs1043227165 CA152224130	96	A>T		No	ClinGen TOPMed gnomAD
CA366517479 rs1195703655	100	A>V		No	ClinGen TOPMed gnomAD
rs754217817 CA4108349	102	V>I		No	ClinGen ExAC TOPMed gnomAD
rs754217817 CA4108348	102	V>L		No	ClinGen ExAC TOPMed gnomAD
rs779443911 CA4108350	105	S>P		No	ClinGen ExAC TOPMed gnomAD
rs148074761 CA4108351	106	T>A		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA4108352 rs750797320	106	T>M		No	ClinGen ExAC TOPMed gnomAD
rs148074761 CA366517512	106	T>P		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs148074761 CA366517513	106	T>S		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA4108354 rs747033709	108	Q>R		No	ClinGen ExAC TOPMed
CA4108355 rs771116530	110	A>T		No	ClinGen ExAC
rs201331220 CA152224166	111	T>A		No	ClinGen 1000Genomes gnomAD
CA366517551 rs1399553263	112	Q>P		No	ClinGen TOPMed
CA4108358 rs749081341	113	G>R		No	ClinGen ExAC gnomAD
rs774242068 CA4108360	115	S>L		No	ClinGen ExAC TOPMed gnomAD
CA366517577 rs1325318897	116	G>V		No	ClinGen Ensembl
CA4108361 rs200427038	118	A>T		No	ClinGen ESP ExAC TOPMed gnomAD
CA4108363 rs772911295	119	P>L		No	ClinGen ExAC gnomAD
CA4108362 rs767501232	119	P>T		No	ClinGen ExAC TOPMed gnomAD
CA152224194 rs908493900	120	Q>E		No	ClinGen Ensembl
rs1562359838 CA366517604	121	L>F		No	ClinGen Ensembl
rs1422934148 CA366517618	123	H>Y		No	ClinGen TOPMed
CA366517645 rs1489716387	127	L>V		No	ClinGen TOPMed gnomAD
rs760224751 CA4108364	128	T>I		No	ClinGen ExAC gnomAD
rs1208150616 CA366517650	128	T>P		No	ClinGen gnomAD
CA4108365 rs760224751	128	T>R		No	ClinGen ExAC gnomAD
rs1475609701 CA366517659	129	T>I		No	ClinGen TOPMed
CA366517654 rs1465545382	129	T>P		No	ClinGen gnomAD
rs941287680 CA152224204	131	P>A		No	ClinGen Ensembl
rs753505824 CA4108366	131	P>H		No	ClinGen ExAC gnomAD
rs199616061 CA4108367	133	P>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA4108369 rs752995266	134	G>C		No	ClinGen ExAC TOPMed gnomAD
CA4108370 rs140089853	136	T>A		No	ClinGen ESP ExAC TOPMed
CA366517697 rs1370130375	136	T>I		No	ClinGen gnomAD
rs778241571 CA4108371	137	P>A		No	ClinGen ExAC TOPMed gnomAD
CA152224228 rs778241571	137	P>T		No	ClinGen ExAC TOPMed gnomAD
CA366517733 rs1583128175	143	M>V		No	ClinGen Ensembl
CA4108376 rs769870393	146	M>I		No	ClinGen ExAC
CA4108375 rs746127880	146	M>T		No	ClinGen ExAC gnomAD
CA152224246 rs1028882699	147	T>S		No	ClinGen TOPMed gnomAD
rs747940424 CA4108378	150	T>P		No	ClinGen ExAC gnomAD
rs773248432 CA4108380	152	A>T		No	ClinGen ExAC gnomAD
rs1329917969 CA366517792	152	A>V		No	ClinGen gnomAD
CA366517799 rs1218192424	153	T>M		No	ClinGen TOPMed
CA366517864 rs1422030633	163	P>L		No	ClinGen gnomAD
CA366517873 rs1412145625	165	L>M		No	ClinGen gnomAD
CA366518171 rs1312590130	170	S>Y		No	ClinGen gnomAD
CA366518232 rs1412175500	175	G>C		No	ClinGen gnomAD
CA366518237 rs1367954958	175	G>V		No	ClinGen Ensembl
CA4108407 rs375063272	176	C>R		No	ClinGen ESP ExAC gnomAD
CA152225202 rs112100346	182	T>A		No	ClinGen Ensembl
CA4108408 rs762389131	182	T>I		No	ClinGen ExAC gnomAD
CA366518368 rs1299337339	184	A>G		No	ClinGen TOPMed
COSM1076233 rs756227958 CA4108411	186	R>C	endometrium haematopoietic_and_lymphoid_tissue [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed
CA366518415 rs1209911056	188	R>*		No	ClinGen gnomAD
rs1419445585 CA366518512	200	V>I		No	ClinGen gnomAD
rs1419445585 CA366518514	200	V>L		No	ClinGen gnomAD
rs1406414133 CA366518541	204	I>L		No	ClinGen TOPMed gnomAD
CA4108442 rs745830790	206	E>A		No	ClinGen ExAC
rs756008941 CA4108443	208	R>*		No	ClinGen ExAC gnomAD
rs1583131728 CA366518572	209	T>P		No	ClinGen Ensembl
rs748740885 CA4108445	210	T>M		No	ClinGen ExAC gnomAD
rs1583131737 CA366518600	213	I>M		No	ClinGen Ensembl
rs887521451 CA152226094	215	S>T		No	ClinGen Ensembl
rs1295992676 CA366518621	216	S>C		No	ClinGen gnomAD
rs748590964 CA366518640	219	M>R		No	ClinGen ExAC gnomAD
rs748590964 CA4108448	219	M>T		No	ClinGen ExAC gnomAD
CA366518648 rs1431326572	220	V>A		No	ClinGen TOPMed
rs1269326640 CA366518659	222	T>A		No	ClinGen gnomAD
rs1188907199 CA366518680	225	K>R		No	ClinGen TOPMed
rs866919321 CA152227236	229	Q>K		No	ClinGen Ensembl
CA4108470 rs747363929	230	S>C		No	ClinGen ExAC gnomAD
CA366518809 rs1333457265	242	Q>*		No	ClinGen gnomAD
CA366518885 rs759710446	252	D>E		No	ClinGen ExAC gnomAD
CA4108473 rs759710446	252	D>E		No	ClinGen ExAC gnomAD
rs1246604155 CA366518917	257	N>H		No	ClinGen TOPMed
CA4108474 rs775603111	258	M>V		No	ClinGen ExAC
CA366518998 rs1206466089	268	I>V		No	ClinGen TOPMed gnomAD
rs1018596766 CA152227283	271	E>D		No	ClinGen TOPMed
rs1008548886 CA152227280	271	E>G		No	ClinGen TOPMed
CA366519030 rs1305796742	273	L>V		No	ClinGen TOPMed
CA366519046 rs1365457491	275	L>P		No	ClinGen TOPMed
rs1298121577 CA366519208	296	I>L		No	ClinGen gnomAD
CA366519227 rs1325232464	298	P>L		No	ClinGen gnomAD
CA366519238 rs1562363181	300	I>F		No	ClinGen Ensembl
CA4108496 rs774531109	305	F>S		No	ClinGen ExAC gnomAD
rs774673727 CA4108497	306	V>I		No	ClinGen ExAC TOPMed gnomAD
rs533679967 CA4108498	308	G>E		No	ClinGen 1000Genomes ExAC gnomAD
rs1420558024 CA366519349	316	K>E		No	ClinGen TOPMed gnomAD
rs904248845 CA366519355	316	K>N		No	ClinGen gnomAD
rs1420558024 CA366519348	316	K>Q		No	ClinGen TOPMed gnomAD
rs1379121744 CA366519381	320	E>D		No	ClinGen gnomAD
rs762934477 CA4108525	328	I>T		No	ClinGen ExAC gnomAD
CA366519595 rs1349833584	336	R>K		No	ClinGen TOPMed
rs1347718096 CA366519602	336	R>S		No	ClinGen gnomAD
rs751047986 CA4108527 COSM1162817	338	T>M	pancreas [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
CA4108529 rs372970346	339	T>M		No	ClinGen ESP ExAC TOPMed gnomAD

No associated diseases with P20226

2 regional properties for P20226

Type	Name	Position	InterPro Accession
domain	Longin domain	4 - 116	IPR010908
domain	v-SNARE, coiled-coil homology domain	124 - 210	IPR042855

Functions

		Description
EC Number	2.3.2.27	Aminoacyltransferases
Subcellular Localization	Cytoplasm, cytosol
PANTHER Family	PTHR21497	UBIQUITIN LIGASE E3 ALPHA-RELATED
PANTHER Subfamily	PTHR21497:SF27	E3 UBIQUITIN-PROTEIN LIGASE UBR1
PANTHER Protein Class	ubiquitin-protein ligase protein modifying enzyme
PANTHER Pathway Category	No pathway information available

11 GO annotations of cellular component

Name	Definition
chromatin	The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
euchromatin	A dispersed and relatively uncompacted form of chromatin that is in a transcription-competent conformation.
female pronucleus	The pronucleus originating from the ovum that is being fertilized.
male pronucleus	The pronucleus originating from the spermatozoa that was involved in fertilization.
nucleoplasm	That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
protein-containing complex	A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
transcription factor TFIIA complex	A component of the transcription machinery of RNA Polymerase II. In humans, TFIIA is a heterotrimer composed of an alpha (P35), beta (P19) and gamma subunits (P12).
transcription factor TFIID complex	A complex composed of TATA binding protein (TBP) and TBP associated factors (TAFs); the total mass is typically about 800 kDa. Most of the TAFs are conserved across species. In TATA-containing promoters for RNA polymerase II (Pol II), TFIID is believed to recognize at least two distinct elements, the TATA element and a downstream promoter element. TFIID is also involved in recognition of TATA-less Pol II promoters. Binding of TFIID to DNA is necessary but not sufficient for transcription initiation from most RNA polymerase II promoters.
transcription preinitiation complex	A protein-DNA complex composed of proteins binding promoter DNA to form the transcriptional preinitiation complex (PIC), the formation of which is a prerequisite for transcription.

12 GO annotations of molecular function

Name	Definition
aryl hydrocarbon receptor binding	Binding to an aryl hydrocarbon receptor.
core promoter sequence-specific DNA binding	Binding to a sequence of DNA that is part of a core promoter region. The core promoter is composed of the transcription start site and binding sites for the RNA polymerase and the basal transcription machinery. The transcribed region might be described as a gene, cistron, or operon.
DNA-binding transcription factor binding	Binding to a DNA-binding transcription factor, a protein that interacts with a specific DNA sequence (sometimes referred to as a motif) within the regulatory region of a gene to modulate transcription.
enzyme binding	Binding to an enzyme, a protein with catalytic activity.
general transcription initiation factor activity	A molecular function required for core promoter activity that mediates the assembly of the RNA polymerase holoenzyme at promoter DNA to form the pre-initiation complex (PIC). General transcription factors (GTFs) bind to and open promoter DNA, initiate RNA synthesis and stimulate the escape of the polymerase from the promoter. Not all subunits of the general transcription factor are necessarily present at all promoters to initiate transcription. GTFs act at each promoter, although the exact subunit composition at individual promoters may vary.
RNA polymerase II cis-regulatory region sequence-specific DNA binding	Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site) located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by RNA polymerase II.
RNA polymerase II core promoter sequence-specific DNA binding	Binding to a DNA sequence that is part of the core promoter of a RNA polymerase II-transcribed gene.
RNA polymerase II general transcription initiation factor activity	A general transcription initiation factor activity that contributes to transcription start site selection and transcription initiation of genes transcribed by RNA polymerase II. The general transcription factors for RNA polymerase II include TFIIB, TFIID, TFIIE, TFIIF, TFIIH and TATA-binding protein (TBP). In most species, RNA polymerase II transcribes all messenger RNAs (mRNAs), most untranslated regulatory RNAs, the majority of the snoRNAs, four of the five snRNAs (U1, U2, U4, and U5), and other small noncoding RNAs. For some small RNAs there is variability between species as to whether it is transcribed by RNA polymerase II or RNA polymerase III. However there are also rare exceptions, such as Trypanosoma brucei, where RNA polymerase I transcribes certain mRNAs in addition to its normal role in rRNA transcription.
RNA polymerase II general transcription initiation factor binding	Binding to a basal RNA polymerase II transcription factor, any of the factors involved in formation of the preinitiation complex (PIC) by RNA polymerase II and defined as a basal or general transcription factor.
RNA polymerase III general transcription initiation factor activity	A general transcription initiation factor activity that contributes to transcription start site selection and transcription initiation of genes transcribed by RNA polymerase III. Factors required for RNA polymerase III transcription initiation include TFIIIA, TFIIIB and TFIIIC. RNA polymerase III transcribes genes encoding short RNAs, including tRNAs, 5S rRNA, U6 snRNA, the short ncRNA component of RNases P, the mitochondrial RNA processing (MRP) RNA, the signal recognition particle SRP RNA, and in higher eukaryotes a number of micro and other small RNAs, though there is some variability across species as to whether a given small noncoding RNA is transcribed by RNA polymerase II or RNA polymerase III.
TFIIB-class transcription factor binding	Binding to a general RNA polymerase II transcription factor of the TFIIB class, one of the factors involved in formation of the preinitiation complex (PIC) by RNA polymerase II.
transcription cis-regulatory region binding	Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon.

8 GO annotations of biological process

Name	Definition
DNA-templated transcription, initiation	The initial step of transcription, consisting of the assembly of the RNA polymerase preinitiation complex (PIC) at a gene promoter, as well as the formation of the first few bonds of the RNA transcript. Transcription initiation includes abortive initiation events, which occur when the first few nucleotides are repeatedly synthesized and then released, and ends when promoter clearance takes place.
mRNA transcription by RNA polymerase II	The cellular synthesis of messenger RNA (mRNA) from a DNA template by RNA polymerase II, originating at an RNA polymerase II promoter.
positive regulation of transcription initiation from RNA polymerase II promoter	Any process that increases the rate, frequency or extent of a process involved in starting transcription from an RNA polymerase II promoter.
protein phosphorylation	The process of introducing a phosphate group on to a protein.
RNA polymerase II preinitiation complex assembly	The aggregation, arrangement and bonding together of proteins on an RNA polymerase II promoter DNA to form the transcriptional preinitiation complex (PIC), the formation of which is a prerequisite for transcription by RNA polymerase.
transcription by RNA polymerase II	The synthesis of RNA from a DNA template by RNA polymerase II (RNAP II), originating at an RNA polymerase II promoter. Includes transcription of messenger RNA (mRNA) and certain small nuclear RNAs (snRNAs).
transcription by RNA polymerase III	The synthesis of RNA from a DNA template by RNA polymerase III, originating at an RNAP III promoter.
transcription initiation from RNA polymerase II promoter	A transcription initiation process that takes place at a RNA polymerase II gene promoter. Messenger RNAs (mRNA) genes, as well as some non-coding RNAs, are transcribed by RNA polymerase II.

24 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P26356	TBP1	TATA-box-binding protein 1	Triticum aestivum (Wheat)	PR
Q02879	TBP2	TATA-box-binding protein 2	Triticum aestivum (Wheat)	PR
P13393	SPT15	TATA-box-binding protein	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	EV
Q2HJ52	TBP	TATA-box-binding protein	Bos taurus (Bovine)	PR
O13270	TBP	TATA-box-binding protein	Gallus gallus (Chicken)	PR
A6H907	TBPL2	TATA box-binding protein-like 2	Pan troglodytes (Chimpanzee)	PR
Q27896	Trf	TBP-related factor	Drosophila melanogaster (Fruit fly)	PR
Q6SJ96	TBPL2	TATA box-binding protein-like 2	Homo sapiens (Human)	PR
P50159	TBP2	TATA-box-binding protein 2	Zea mays (Maize)	PR
P50158	TBP1	TATA-box-binding protein 1	Zea mays (Maize)	PR
Q6SJ95	Tbpl2	TATA box-binding protein-like 2	Mus musculus (Mouse)	PR
P29037	Tbp	TATA-box-binding protein	Mus musculus (Mouse)	PR
P26357	TBP	TATA-box-binding protein	Solanum tuberosum (Potato)	PR
A6H909	Tbpl2	TATA box-binding protein-like 2	Rattus norvegicus (Rat)	PR
Q8W0W4	TBP2	TATA-binding protein 2	Oryza sativa subsp japonica (Rice)	PR
B2D6P4	tlf-1	TATA box-binding protein-like 1	Caenorhabditis elegans	SS
O45211	Tbp	TATA-box-binding protein	Bombyx mori (Silk moth)	PR
Q42808	TBP1	TATA-box-binding protein	Glycine max (Soybean) (Glycine hispida)	PR
P28148	TBP2	TATA-box-binding protein 2	Arabidopsis thaliana (Mouse-ear cress)	PR
P28147	TBP1	TATA-box-binding protein 1	Arabidopsis thaliana (Mouse-ear cress)	PR
Q28DH2	tbpl2	TATA box-binding protein-like 2	Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)	PR
Q28GG8	tbp	TATA-box-binding protein	Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)	PR
Q1JPY4	tbpl2	TATA box-binding protein-like 2	Danio rerio (Zebrafish) (Brachydanio rerio)	PR
Q7SXL3	tbp	TATA-box-binding protein	Danio rerio (Zebrafish) (Brachydanio rerio)	PR

10	20	30	40	50	60
MADEEMDGAE	RMDVSPEPPL	APQRPASWWD	QQVDFYTAFL	HHLAQLVPEI	YFAEMDPDLE
70	80	90	100	110	120
KQEESVQMSI	LTPLEWYLFG	EDPDICLEKL	KHSGAFQLCG	KVFKSGETTY	SCRDCAIDPT
130	140	150	160	170	180
CVLCMDCFQS	SVHKNHRYKM	HTSTGGGFCD	CGDTEAWKTG	PFCVDHEPGR	AGTTKESLHC
190	200	210	220	230	240
PLNEEVIAQA	RRIFPSVIKY	IVEMTIWEEE	KELPPELQIR	EKNERYYCVL	FNDEHHSYDH
250	260	270	280	290	300
VIYSLQRALD	CELAEAQLHT	TAIDKEGRRA	VKAGVYATCQ	EAKEDIKSHS	ENVSQHPLHV
310	320	330	340	350	360
EVLHSVVMAH	QKFALRLGSW	MNKIMSYSSD	FRQIFCQACL	VEEPGSENPC	LISRLMLWDA
370	380	390	400	410	420
KLYKGARKIL	HELIFSSFFM	EMEYKKLFAM	EFVKYYKQLQ	KEYISDDHER	SISITALSVQ
430	440	450	460	470	480
MLTVPTLARH	LIEEQNVISV	ITETLLEVLP	EYLDRNNKFN	FQGYSQDKLG	RVYAVICDLK
490	500	510	520	530	540
YILISKPVIW	TERLRAQFLE	GFRSFLKILT	CMQGMEEIRR	QVGQHIEVDP	DWEAAIAIQM
550	560	570	580	590	600
QLKNILLMFQ	EWCACDEDLL	LVAYKECHKA	VMRCSTNFMS	STKTVVQLCG	HSLETKSYKV
610	620	630	640	650	660
SEDLVSIHLP	LSRTLAGLHV	RLSRLGAISR	LHEFVPFDGF	QVEVLVEYPL	RCLVLVAQVV
670	680	690	700	710	720
AEMWRRNGLS	LISQVFYYQD	VKCREEMYDK	DIIMLQIGAS	IMDPNKFLLL	VLQRYELTDA
730	740	750	760	770	780
FNKTISTKDQ	DLIKQYNTLI	EEMLQVLIYI	VGERYVPGVG	NVTREEVIMR	EITHLLCIEP
790	800	810	820	830	840
MPHSAIARNL	PENENNETGL	ENVINKVATF	KKPGVSGHGV	YELKDESLKD	FNMYFYHYSK
850	860	870	880	890	900
TQHSKAEHMQ	KKRRKQENKD	EALPPPPPPE	FCPAFSKVVN	LLSCDVMMYI	LRTIFERAVD
910	920	930	940	950	960
MESNLWTEGM	LQMAFHILAL	GLLEEKQQLQ	KAPEEEVAFD	FYHKASRLGS	SAMNAQNIQM
970	980	990	1000	1010	1020
LLEKLKGIPQ	LESQKDMITW	ILQMFDTVKR	LREKSCLVVA	TTSGLECVKS	EEITHDKEKA
1030	1040	1050	1060	1070	1080
ERKRKAEAAR	LHRQKIMAQM	SALQKNFIET	HKLMYDNTSE	VTGKEDSIME	EESTSAVSEA
1090	1100	1110	1120	1130	1140
SRIALGPKRG	PAVTEKEVLT	CILCQEEQEV	KLENNAMVLS	ACVQKSTALT	QHRGKPVDHL
1150	1160	1170	1180	1190	1200
GETLDPLFMD	PDLAHGTYTG	SCGHVMHAVC	WQKYFEAVQL	SSQQRIHVDL	FDLESGEYLC
1210	1220	1230	1240	1250	1260
PLCKSLCNTV	IPIIPLQPQK	INSENAEALA	QLLTLARWIQ	TVLARISGYN	IKHAKGEAPA
1270	1280	1290	1300	1310	1320
VPVLFNQGMG	DSTFEFHSIL	SFGVQSSVKY	SNSIKEMVIL	FATTIYRIGL	KVPPDELDPR
1330	1340	1350	1360	1370	1380
VPMMTWSTCA	FTIQAIENLL	GDEGKPLFGA	LQNRQHNGLK	ALMQFAVAQR	TTCPQVLIHK
1390	1400	1410	1420	1430	1440
HLARLLSVIL	PNLQSENTPG	LLSVDLFHVL	VGAVLAFPSL	YWDDTVDLQP	SPLSSSYNHL
1450	1460	1470	1480	1490	1500
YLFHLITMAH	MLQILLTTDT	DLSSGPPLAE	GEEDSEEARC	ASAFFVEVSQ	HTDGLAGCGA
1510	1520	1530	1540	1550	1560
PGWYLWLSLR	NGITPYLRCA	ALLFHYLLGV	APPEELFANS	AEGEFSALCS	YLSLPTNLFL
1570	1580	1590	1600	1610	1620
LFQEYWDTIR	PLLQRWCGDP	ALLKSLKQKS	AVVRYPRKRN	SLIELPEDYS	CLLNQASHFR
1630	1640	1650	1660	1670	1680
CPRSADDERK	HPVLCLFCGA	ILCSQNICCQ	EIVNGEEVGA	CVFHALHCGA	GVCIFLKIRE
1690	1700	1710	1720	1730	1740
CRVVLVEGKA	RGCAYPAPYL	DEYGETDPGL	KRGNPLHLSR	ERYRKLHLVW	QQHCIIEEIA
1750
RSQETNQMLF	GFNWQLL