P16521

PR
Gene name
YEF3 (EFC1, TEF3, YEF3A, YLR249W, L9672.5)
Protein name
Elongation factor 3A
Names
EF-3, EF-3A, Eukaryotic elongation factor 3, eEF3, Translation elongation factor 3A, Yeast elongation factor 3
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YLR249W
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

371-447 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

6 structures for P16521

Entry ID Method Resolution Chain Position Source
2IW3 X-ray 240 A A/B 2-981 PDB
2IWH X-ray 300 A A/B 2-981 PDB
2IX3 X-ray 270 A A/B 2-981 PDB
2IX8 EM 600 A A 2-977 PDB
7B7D EM 330 A EF 1-1044 PDB
AF-P16521-F1 Predicted AlphaFoldDB

5 variants for P16521

Variant ID(s) Position Change Description Diseaes Association Provenance
s12-636839 20 V>I No SGRP
s12-637223 148 A>T No SGRP
s12-637238 153 I>F No SGRP
s12-637349 190 A>S No SGRP
s12-637775 332 V>L No SGRP

No associated diseases with P16521

9 regional properties for P16521

Type Name Position InterPro Accession
domain ABC transporter-like, ATP-binding domain 426 - 641 IPR003439-1
domain ABC transporter-like, ATP-binding domain 667 - 993 IPR003439-2
domain AAA+ ATPase domain 455 - 616 IPR003593-1
domain AAA+ ATPase domain 693 - 970 IPR003593-2
domain Elongation Factor 3, ABC2 domain, chromodomain-like insertion 794 - 861 IPR015688
conserved_site ABC transporter-like, conserved site 544 - 558 IPR017871-1
conserved_site ABC transporter-like, conserved site 897 - 911 IPR017871-2
repeat HEAT, type 2 171 - 209 IPR021133
domain Elongation factor 3, four helical bundle domain 336 - 411 IPR040533

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
cytoplasmic stress granule A dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress.
cytosolic ribosome A ribosome located in the cytosol.
polysomal ribosome A ribosome bound to mRNA that forms part of a polysome.
ribosome An intracellular organelle, about 200 A in diameter, consisting of RNA and protein. It is the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). It consists of two subunits, one large and one small, each containing only protein and RNA. Both the ribosome and its subunits are characterized by their sedimentation coefficients, expressed in Svedberg units (symbol: S). Hence, the prokaryotic ribosome (70S) comprises a large (50S) subunit and a small (30S) subunit, while the eukaryotic ribosome (80S) comprises a large (60S) subunit and a small (40S) subunit. Two sites on the ribosomal large subunit are involved in translation, namely the aminoacyl site (A site) and peptidyl site (P site). Ribosomes from prokaryotes, eukaryotes, mitochondria, and chloroplasts have characteristically distinct ribosomal proteins.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
rRNA binding Binding to a ribosomal RNA.
translation elongation factor activity Functions in chain elongation during polypeptide synthesis at the ribosome.

4 GO annotations of biological process

Name Definition
negative regulation of protein kinase activity Any process that stops, prevents, or reduces the frequency, rate or extent of protein kinase activity.
negative regulation of protein phosphorylation Any process that stops, prevents or reduces the rate of addition of phosphate groups to amino acids within a protein.
translational elongation The successive addition of amino acid residues to a nascent polypeptide chain during protein biosynthesis.
translational termination The process resulting in the release of a polypeptide chain from the ribosome, usually in response to a termination codon (UAA, UAG, or UGA in the universal genetic code).

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P0A9U3 ybiT Probable ATP-binding protein YbiT Escherichia coli (strain K12) PR
P43672 uup ATP-binding protein Uup Escherichia coli (strain K12) PR
10 20 30 40 50 60
MSDSQQSIKV LEELFQKLSV ATADNRHEIA SEVASFLNGN IIEHDVPEHF FGELAKGIKD
70 80 90 100 110 120
KKTAANAMQA VAHIANQSNL SPSVEPYIVQ LVPAICTNAG NKDKEIQSVA SETLISIVNA
130 140 150 160 170 180
VNPVAIKALL PHLTNAIVET NKWQEKIAIL AAISAMVDAA KDQVALRMPE LIPVLSETMW
190 200 210 220 230 240
DTKKEVKAAA TAAMTKATET VDNKDIERFI PSLIQCIADP TEVPETVHLL GATTFVAEVT
250 260 270 280 290 300
PATLSIMVPL LSRGLNERET GIKRKSAVII DNMCKLVEDP QVIAPFLGKL LPGLKSNFAT
310 320 330 340 350 360
IADPEAREVT LRALKTLRRV GNVGEDDAIP EVSHAGDVST TLQVVNELLK DETVAPRFKI
370 380 390 400 410 420
VVEYIAAIGA DLIDERIIDQ QAWFTHITPY MTIFLHEKKA KDILDEFRKR AVDNIPVGPN
430 440 450 460 470 480
FDDEEDEGED LCNCEFSLAY GAKILLNKTQ LRLKRARRYG ICGPNGCGKS TLMRAIANGQ
490 500 510 520 530 540
VDGFPTQEEC RTVYVEHDID GTHSDTSVLD FVFESGVGTK EAIKDKLIEF GFTDEMIAMP
550 560 570 580 590 600
ISALSGGWKM KLALARAVLR NADILLLDEP TNHLDTVNVA WLVNYLNTCG ITSITISHDS
610 620 630 640 650 660
VFLDNVCEYI INYEGLKLRK YKGNFTEFVK KCPAAKAYEE LSNTDLEFKF PEPGYLEGVK
670 680 690 700 710 720
TKQKAIVKVT NMEFQYPGTS KPQITDINFQ CSLSSRIAVI GPNGAGKSTL INVLTGELLP
730 740 750 760 770 780
TSGEVYTHEN CRIAYIKQHA FAHIESHLDK TPSEYIQWRF QTGEDRETMD RANRQINEND
790 800 810 820 830 840
AEAMNKIFKI EGTPRRIAGI HSRRKFKNTY EYECSFLLGE NIGMKSERWV PMMSVDNAWI
850 860 870 880 890 900
PRGELVESHS KMVAEVDMKE ALASGQFRPL TRKEIEEHCS MLGLDPEIVS HSRIRGLSGG
910 920 930 940 950 960
QKVKLVLAAG TWQRPHLIVL DEPTNYLDRD SLGALSKALK EFEGGVIIIT HSAEFTKNLT
970 980 990 1000 1010 1020
EEVWAVKDGR MTPSGHNWVS GQGAGPRIEK KEDEEDKFDA MGNKIAGGKK KKKLSSAELR
1030 1040
KKKKERMKKK KELGDAYVSS DEEF