P07379

PR
Gene name
Pck1
Protein name
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Names
PEPCK-C, Serine-protein kinase PCK1
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:362282
EC number
4.1.1.32: Carboxy-lyases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-46 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

47 structures for P07379

Entry ID Method Resolution Chain Position Source
2QEW X-ray 180 A A 1-622 PDB
2QEY X-ray 190 A A 1-622 PDB
2QF1 X-ray 180 A A 1-622 PDB
2QF2 X-ray 165 A A/B 1-622 PDB
2RK7 X-ray 190 A A/B 1-622 PDB
2RK8 X-ray 200 A A/B 1-622 PDB
2RKA X-ray 195 A A/C 1-622 PDB
2RKD X-ray 190 A A 1-622 PDB
2RKE X-ray 180 A A 1-622 PDB
3DT2 X-ray 150 A A 1-622 PDB
3DT4 X-ray 145 A A/C 1-622 PDB
3DT7 X-ray 150 A A/B 1-622 PDB
3DTB X-ray 130 A A/B 1-622 PDB
3MOE X-ray 125 A A 1-622 PDB
3MOF X-ray 175 A A/B 1-622 PDB
3MOH X-ray 210 A A/B 1-622 PDB
4GMM X-ray 174 A PDB
4GMU X-ray 120 A PDB
4GMW X-ray 175 A PDB
4GMZ X-ray 205 A PDB
4GNL X-ray 170 A PDB
4GNM X-ray 150 A PDB
4GNO X-ray 150 A PDB
4GNP X-ray 174 A PDB
4GNQ X-ray 140 A PDB
4OX2 X-ray 200 A A/B 1-622 PDB
4YW8 X-ray 155 A A 1-622 PDB
4YW9 X-ray 140 A A 1-622 PDB
4YWB X-ray 150 A A/C 1-622 PDB
4YWD X-ray 210 A A 1-622 PDB
5FH0 X-ray 160 A A 1-622 PDB
5FH1 X-ray 155 A A 1-622 PDB
5FH2 X-ray 149 A A 1-622 PDB
5FH3 X-ray 160 A A 1-622 PDB
5FH4 X-ray 149 A A 1-622 PDB
5FH5 X-ray 155 A A 1-622 PDB
5V95 X-ray 230 A A 1-622 PDB
5V97 X-ray 180 A A 1-622 PDB
5V9F X-ray 205 A A 1-622 PDB
5V9G X-ray 195 A A 1-622 PDB
5V9H X-ray 215 A A/B 1-622 PDB
6P5O X-ray 149 A A 1-622 PDB
6YI9 X-ray 175 A A 1-622 PDB
7L36 X-ray 184 A A 1-622 PDB
7L3M X-ray 207 A A 1-622 PDB
7L3V X-ray 198 A A 1-622 PDB
AF-P07379-F1 Predicted AlphaFoldDB

1 variants for P07379

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3320080945 112 Q>H No EVA

No associated diseases with P07379

3 regional properties for P07379

Type Name Position InterPro Accession
conserved_site Phosphoenolpyruvate carboxykinase, GTP-utilising, conserved site 284 - 292 IPR018091
domain Phosphoenolpyruvate carboxykinase, C-terminal P-loop domain 260 - 618 IPR035077
domain Phosphoenolpyruvate carboxykinase, GTP-utilising, N-terminal 29 - 256 IPR035078

Functions

Description
EC Number 4.1.1.32 Carboxy-lyases
Subcellular Localization
  • Cytoplasm, cytosol
  • Endoplasmic reticulum
  • Phosphorylation at Ser-90 promotes translocation to the endoplasmic reticulum
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
endoplasmic reticulum The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached).
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

9 GO annotations of molecular function

Name Definition
carboxylic acid binding Binding to a carboxylic acid, an organic acid containing one or more carboxyl (COOH) groups or anions (COO-).
GDP binding Binding to GDP, guanosine 5'-diphosphate.
GTP binding Binding to GTP, guanosine triphosphate.
magnesium ion binding Binding to a magnesium (Mg) ion.
manganese ion binding Binding to a manganese ion (Mn).
nucleoside diphosphate kinase activity Catalysis of the reaction: ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
phosphoenolpyruvate carboxykinase (GTP) activity Catalysis of the reaction: GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.
phosphoenolpyruvate carboxykinase activity Catalysis of the reaction: source of phosphate + oxaloacetate = phosphoenolpyruvate + CO2 + other reaction products.
protein serine kinase activity (using GTP as donor) Catalysis of the reactions: GTP + L-seryl- = GDP + H(+) + O-phospho-L-seryl-

36 GO annotations of biological process

Name Definition
aging A developmental process that is a deterioration and loss of function over time. Aging includes loss of functions such as resistance to disease, homeostasis, and fertility, as well as wear and tear. Aging includes cellular senescence, but is more inclusive. May precede death and may succeed developmental maturation (GO:0021700).
cellular hyperosmotic salinity response Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detection of, or exposure to, an increase in the concentration of salt (particularly but not exclusively sodium and chloride ions) in the environment.
cellular hypotonic salinity response Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detection of, or exposure to, a decrease in the concentration of salt (particularly but not exclusively sodium and chloride ions) in the environment.
cellular response to cAMP Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cAMP (cyclic AMP, adenosine 3',5'-cyclophosphate) stimulus.
cellular response to dexamethasone stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a dexamethasone stimulus.
cellular response to fructose stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a fructose stimulus.
cellular response to glucagon stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucagon stimulus.
cellular response to glucose stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucose stimulus.
cellular response to hypoxia Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
cellular response to insulin stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin stimulus. Insulin is a polypeptide hormone produced by the islets of Langerhans of the pancreas in mammals, and by the homologous organs of other organisms.
cellular response to interleukin-1 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an interleukin-1 stimulus.
cellular response to phorbol 13-acetate 12-myristate Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a phorbol 13-acetate 12-myristate stimulus.
cellular response to potassium ion starvation Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of potassium ions.
cellular response to raffinose Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a raffinose stimulus.
cellular response to retinoic acid Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a retinoic acid stimulus.
cellular response to tumor necrosis factor Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a tumor necrosis factor stimulus.
gluconeogenesis The formation of glucose from noncarbohydrate precursors, such as pyruvate, amino acids and glycerol.
glucose homeostasis Any process involved in the maintenance of an internal steady state of glucose within an organism or cell.
glucose metabolic process The chemical reactions and pathways involving glucose, the aldohexose gluco-hexose. D-glucose is dextrorotatory and is sometimes known as dextrose; it is an important source of energy for living organisms and is found free as well as combined in homo- and hetero-oligosaccharides and polysaccharides.
glycerol biosynthetic process from pyruvate The chemical reactions and pathways resulting in the formation of glycerol, 1,2,3-propanetriol, from other compounds, including pyruvate.
hepatocyte differentiation The process in which a relatively unspecialized cell acquires the specialized features of a hepatocyte. A hepatocyte is specialized epithelial cell that is organized into interconnected plates called lobules, and is the main structural component of the liver.
lipid metabolic process The chemical reactions and pathways involving lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent. Includes fatty acids; neutral fats, other fatty-acid esters, and soaps; long-chain (fatty) alcohols and waxes; sphingoids and other long-chain bases; glycolipids, phospholipids and sphingolipids; and carotenes, polyprenols, sterols, terpenes and other isoprenoids.
oxaloacetate metabolic process The chemical reactions and pathways involving oxaloacetate, the anion of oxobutanedioic acid, an important intermediate in metabolism, especially as a component of the TCA cycle.
peptidyl-serine phosphorylation The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
positive regulation of memory T cell differentiation Any process that activates or increases the frequency, rate or extent of memory T cell differentiation.
positive regulation of transcription from RNA polymerase II promoter in response to acidic pH Any process that increases the frequency, rate or extent of transcription from an RNA polymerase II promoter as a result of a pH stimulus with pH < 7.
propionate catabolic process The chemical reactions and pathways resulting in the breakdown of propionate, the anion derived from propionic acid.
regulation of lipid biosynthetic process Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of lipids.
response to activity Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an activity stimulus.
response to bacterium Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a bacterium.
response to insulin Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin stimulus. Insulin is a polypeptide hormone produced by the islets of Langerhans of the pancreas in mammals, and by the homologous organs of other organisms.
response to interleukin-6 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an interleukin-6 stimulus.
response to lipid Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipid stimulus.
response to lipopolysaccharide Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria.
response to methionine Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a methionine stimulus.
response to starvation Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a starvation stimulus, deprivation of nourishment.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P05153 PCK1 Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Gallus gallus (Chicken) PR
P35558 PCK1 Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Homo sapiens (Human) PR
Q9Z2V4 Pck1 Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Mus musculus (Mouse) PR
10 20 30 40 50 60
MPPQLHNGLD FSAKVIQGSL DSLPQEVRKF VEGNAQLCQP EYIHICDGSE EEYGRLLAHM
70 80 90 100 110 120
QEEGVIRKLK KYDNCWLALT DPRDVARIES KTVIITQEQR DTVPIPKSGQ SQLGRWMSEE
130 140 150 160 170 180
DFEKAFNARF PGCMKGRTMY VIPFSMGPLG SPLAKIGIEL TDSPYVVASM RIMTRMGTSV
190 200 210 220 230 240
LEALGDGEFI KCLHSVGCPL PLKKPLVNNW ACNPELTLIA HLPDRREIIS FGSGYGGNSL
250 260 270 280 290 300
LGKKCFALRI ASRLAKEEGW LAEHMLILGI TNPEGKKKYL AAAFPSACGK TNLAMMNPTL
310 320 330 340 350 360
PGWKVECVGD DIAWMKFDAQ GNLRAINPEN GFFGVAPGTS VKTNPNAIKT IQKNTIFTNV
370 380 390 400 410 420
AETSDGGVYW EGIDEPLAPG VTITSWKNKE WRPQDEEPCA HPNSRFCTPA SQCPIIDPAW
430 440 450 460 470 480
ESPEGVPIEG IIFGGRRPAG VPLVYEALSW QHGVFVGAAM RSEATAAAEH KGKVIMHDPF
490 500 510 520 530 540
AMRPFFGYNF GKYLAHWLSM AHRPAAKLPK IFHVNWFRKD KNGKFLWPGF GENSRVLEWM
550 560 570 580 590 600
FGRIEGEDSA KLTPIGYVPK EDALNLKGLG DVNVEELFGI SKEFWEKEVE EIDKYLEDQV
610 620
NADLPYEIER ELRALKQRIS QM