P06245

PR
Gene name
TPK2 (PKA2, YKR1, YPL203W)
Protein name
cAMP-dependent protein kinase type 2
Names
PKA 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YPL203W
EC number
2.7.11.11: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-89 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

210-230 (Activation loop from InterPro)

Target domain

70-324 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P06245

Entry ID Method Resolution Chain Position Source
AF-P06245-F1 Predicted AlphaFoldDB

5 variants for P06245

Variant ID(s) Position Change Description Diseaes Association Provenance
s16-166273 7 R>G No SGRP
s16-166317 21 N>K No SGRP
s16-166457 68 H>R No SGRP
s16-166976 241 P>L No SGRP
s16-167335 361 E>K No SGRP

No associated diseases with P06245

4 regional properties for P06245

Type Name Position InterPro Accession
domain Protein kinase domain 70 - 324 IPR000719
domain AGC-kinase, C-terminal 325 - 380 IPR000961
active_site Serine/threonine-protein kinase, active site 189 - 201 IPR008271
binding_site Protein kinase, ATP binding site 76 - 99 IPR017441

Functions

Description
EC Number 2.7.11.11 Protein-serine/threonine kinases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cAMP-dependent protein kinase complex An enzyme complex, composed of regulatory and catalytic subunits, that catalyzes protein phosphorylation. Inactive forms of the enzyme have two regulatory chains and two catalytic chains; activation by cAMP produces two active catalytic monomers and a regulatory dimer.
chromatin The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
P-body A focus in the cytoplasm where mRNAs may become inactivated by decapping or some other mechanism. Protein and RNA localized to these foci are involved in mRNA degradation, nonsense-mediated mRNA decay (NMD), translational repression, and RNA-mediated gene silencing.

6 GO annotations of molecular function

Name Definition
AMP-activated protein kinase activity Catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein. This reaction requires the presence of AMP.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
cAMP-dependent protein kinase activity cAMP-dependent catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
translation initiation factor binding Binding to a translation initiation factor, any polypeptide factor involved in the initiation of ribosome-mediated translation.

6 GO annotations of biological process

Name Definition
invasive growth in response to glucose limitation A growth pattern exhibited by budding haploid cells under certain growth conditions, in which cells retain the typical axial budding pattern of haploids, but become elongated and fail to separate after division; during growth on a solid substrate, this results in penetration of cells into the agar medium. An example of this process is found in Saccharomyces cerevisiae.
negative regulation of cytoplasmic mRNA processing body assembly Any process that decreases the rate, frequency, or extent of the aggregation, arrangement and bonding together of proteins and RNA molecules to form a cytoplasmic mRNA processing body.
negative regulation of cytoplasmic translation Any process that stops, prevents or reduces the frequency, rate or extent of cytoplasmic translation.
protein kinase A signaling A series of reactions, mediated by the intracellular serine/threonine kinase protein kinase A, which occurs as a result of a single trigger reaction or compound.
protein phosphorylation The process of introducing a phosphate group on to a protein.
Ras protein signal transduction The series of molecular signals within the cell that are mediated by a member of the Ras superfamily of proteins switching to a GTP-bound active state.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P06244 TPK1 cAMP-dependent protein kinase type 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P05986 TPK3 cAMP-dependent protein kinase type 3 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
10 20 30 40 50 60
MEFVAERAQP VGQTIQQQNV NTYGQGVLQP HHDLQQRQQQ QQQRQHQQLL TSQLPQKSLV
70 80 90 100 110 120
SKGKYTLHDF QIMRTLGTGS FGRVHLVRSV HNGRYYAIKV LKKQQVVKMK QVEHTNDERR
130 140 150 160 170 180
MLKLVEHPFL IRMWGTFQDA RNIFMVMDYI EGGELFSLLR KSQRFPNPVA KFYAAEVILA
190 200 210 220 230 240
LEYLHAHNII YRDLKPENIL LDRNGHIKIT DFGFAKEVQT VTWTLCGTPD YIAPEVITTK
250 260 270 280 290 300
PYNKSVDWWS LGVLIYEMLA GYTPFYDTTP MKTYEKILQG KVVYPPYFHP DVVDLLSKLI
310 320 330 340 350 360
TADLTRRIGN LQSGSRDIKA HPWFSEVVWE RLLAKDIETP YEPPITSGIG DTSLFDQYPE
370
EQLDYGIQGD DPYAEYFQDF