P05986

PR
Gene name
TPK3 (PKA3, YKL166C, YKL630)
Protein name
cAMP-dependent protein kinase type 3
Names
PKA 3
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YKL166C
EC number
2.7.11.11: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-104 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

228-248 (Activation loop from InterPro)

Target domain

88-342 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P05986

Entry ID Method Resolution Chain Position Source
AF-P05986-F1 Predicted AlphaFoldDB

4 variants for P05986

Variant ID(s) Position Change Description Diseaes Association Provenance
s11-135586 42 C>F No SGRP
s11-135480 77 R>S No SGRP
s11-135088 208 I>T No SGRP
s11-134740 324 E>G No SGRP

No associated diseases with P05986

4 regional properties for P05986

Type Name Position InterPro Accession
domain Protein kinase domain 88 - 342 IPR000719
domain AGC-kinase, C-terminal 343 - 398 IPR000961
active_site Serine/threonine-protein kinase, active site 207 - 219 IPR008271
binding_site Protein kinase, ATP binding site 94 - 126 IPR017441

Functions

Description
EC Number 2.7.11.11 Protein-serine/threonine kinases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cAMP-dependent protein kinase complex An enzyme complex, composed of regulatory and catalytic subunits, that catalyzes protein phosphorylation. Inactive forms of the enzyme have two regulatory chains and two catalytic chains; activation by cAMP produces two active catalytic monomers and a regulatory dimer.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic stress granule A dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
P-body A focus in the cytoplasm where mRNAs may become inactivated by decapping or some other mechanism. Protein and RNA localized to these foci are involved in mRNA degradation, nonsense-mediated mRNA decay (NMD), translational repression, and RNA-mediated gene silencing.

5 GO annotations of molecular function

Name Definition
AMP-activated protein kinase activity Catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein. This reaction requires the presence of AMP.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
cAMP-dependent protein kinase activity cAMP-dependent catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.

6 GO annotations of biological process

Name Definition
mitochondrion organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a mitochondrion; includes mitochondrial morphogenesis and distribution, and replication of the mitochondrial genome as well as synthesis of new mitochondrial components.
negative regulation of cytoplasmic mRNA processing body assembly Any process that decreases the rate, frequency, or extent of the aggregation, arrangement and bonding together of proteins and RNA molecules to form a cytoplasmic mRNA processing body.
negative regulation of cytoplasmic translation Any process that stops, prevents or reduces the frequency, rate or extent of cytoplasmic translation.
protein kinase A signaling A series of reactions, mediated by the intracellular serine/threonine kinase protein kinase A, which occurs as a result of a single trigger reaction or compound.
protein phosphorylation The process of introducing a phosphate group on to a protein.
Ras protein signal transduction The series of molecular signals within the cell that are mediated by a member of the Ras superfamily of proteins switching to a GTP-bound active state.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P06244 TPK1 cAMP-dependent protein kinase type 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P06245 TPK2 cAMP-dependent protein kinase type 2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
10 20 30 40 50 60
MYVDPMNNNE IRKLSITAKT ETTPDNVGQD IPVNAHSVHE ECSSNTPVEI NGRNSGKLKE
70 80 90 100 110 120
EASAGICLVK KPMLQYRDTS GKYSLSDFQI LRTLGTGSFG RVHLIRSNHN GRFYALKTLK
130 140 150 160 170 180
KHTIVKLKQV EHTNDERRML SIVSHPFIIR MWGTFQDSQQ VFMVMDYIEG GELFSLLRKS
190 200 210 220 230 240
QRFPNPVAKF YAAEVCLALE YLHSKDIIYR DLKPENILLD KNGHIKITDF GFAKYVPDVT
250 260 270 280 290 300
YTLCGTPDYI APEVVSTKPY NKSVDWWSFG VLIYEMLAGY TPFYNSNTMK TYENILNAEL
310 320 330 340 350 360
KFPPFFHPDA QDLLKKLITR DLSERLGNLQ NGSEDVKNHP WFNEVIWEKL LARYIETPYE
370 380 390
PPIQQGQGDT SQFDRYPEEE FNYGIQGEDP YMDLMKEF