P04786

PR
Gene name
TOP1 (MAK1, YOL006C)
Protein name
DNA topoisomerase 1
Names
DNA topoisomerase I, Maintenance of killer protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YOL006C
EC number
5.6.2.1: Enzymes altering nucleic acid conformation
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-166 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for P04786

Entry ID Method Resolution Chain Position Source
1OIS X-ray 190 A A 141-363 PDB
AF-P04786-F1 Predicted AlphaFoldDB

7 variants for P04786

Variant ID(s) Position Change Description Diseaes Association Provenance
s15-315192 66 T>S No SGRP
s15-315088 101 T>A No SGRP
s15-315055 112 K>E No SGRP
s15-314653 246 E>K No SGRP
s15-314188 401 V>L No SGRP
s15-313863 509 P>L No SGRP
s15-313657 578 Q>E No SGRP

No associated diseases with P04786

5 regional properties for P04786

Type Name Position InterPro Accession
domain DNA topoisomerase I, DNA binding, eukaryotic-type 143 - 361 IPR008336
domain DNA topoisomerase I, eukaryotic-type 292 - 741 IPR013499
domain DNA topoisomerase I, catalytic core, eukaryotic-type 364 - 589 IPR013500
active_site DNA topoisomerase I, active site 714 - 732 IPR018521
domain Topoisomerase I C-terminal domain 699 - 769 IPR025834

Functions

Description
EC Number 5.6.2.1 Enzymes altering nucleic acid conformation
Subcellular Localization
  • Nucleus, nucleolus
  • Nucleus, nucleoplasm
  • Diffuse nuclear localization with some enrichment in nucleoli
  • On CPT treatment, cleared from nucleoli into nucleoplasm
  • Sumoylated forms found in both nucleoplasm and nucleoli (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
replication fork protection complex A protein complex conserved in eukaryotes and associated with the replication fork; the complex stabilizes stalled replication forks and is thought to be involved in coordinating leading- and lagging-strand synthesis and in replication checkpoint signaling.

2 GO annotations of molecular function

Name Definition
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA topoisomerase type I (single strand cut, ATP-independent) activity Catalysis of a DNA topological transformation by transiently cleaving one DNA strand at a time to allow passage of another strand; changes the linking number by +1 per catalytic cycle.

12 GO annotations of biological process

Name Definition
chromatin organization The assembly or remodeling of chromatin composed of DNA complexed with histones, other associated proteins, and sometimes RNA.
chromosome segregation The process in which genetic material, in the form of chromosomes, is organized into specific structures and then physically separated and apportioned to two or more sets. In eukaryotes, chromosome segregation begins with the condensation of chromosomes, includes chromosome separation, and ends when chromosomes have completed movement to the spindle poles.
DNA replication The cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by initiation proteins, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA.
DNA strand elongation involved in DNA replication The process in which an existing DNA strand is extended by activities including the addition of nucleotides to the 3' end of the strand, complementary to an existing template, as part of DNA replication.
DNA topological change The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number.
mitotic chromosome condensation The cell cycle process in which chromatin structure is compacted prior to and during mitosis in eukaryotic cells.
nuclear migration The directed movement of the nucleus to a specific location within a cell.
rDNA heterochromatin assembly The formation of heterochromatin at ribosomal DNA, characterized by the modified histone H3K9me3.
regulation of mitotic recombination Any process that modulates the frequency, rate or extent of DNA recombination during mitosis.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
rRNA transcription The synthesis of ribosomal RNA (rRNA), any RNA that forms part of the ribosomal structure, from a DNA template.
transcription elongation by RNA polymerase II promoter The extension of an RNA molecule after transcription initiation and promoter clearance at an RNA polymerase II promoter by the addition of ribonucleotides catalyzed by RNA polymerase II.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P11387 TOP1 DNA topoisomerase 1 Homo sapiens (Human) PR
Q04750 Top1 DNA topoisomerase 1 Mus musculus (Mouse) PR
10 20 30 40 50 60
MTIADASKVN HELSSDDDDD VPLSQTLKKR KVASMNSASL QDEAEPYDSD EAISKISKKK
70 80 90 100 110 120
TKKIKTEPVQ SSSLPSPPAK KSATSKPKKI KKEDGDVKVK TTKKEEQENE KKKREEEEEE
130 140 150 160 170 180
DKKAKEEEEE YKWWEKENED DTIKWVTLKH NGVIFPPPYQ PLPSHIKLYY DGKPVDLPPQ
190 200 210 220 230 240
AEEVAGFFAA LLESDHAKNP VFQKNFFNDF LQVLKESGGP LNGIEIKEFS RCDFTKMFDY
250 260 270 280 290 300
FQLQKEQKKQ LTSQEKKQIR LEREKFEEDY KFCELDGRRE QVGNFKVEPP DLFRGRGAHP
310 320 330 340 350 360
KTGKLKRRVN PEDIVLNLSK DAPVPPAPEG HKWGEIRHDN TVQWLAMWRE NIFNSFKYVR
370 380 390 400 410 420
LAANSSLKGQ SDYKKFEKAR QLKSYIDAIR RDYTRNLKSK VMLERQKAVA IYLIDVFALR
430 440 450 460 470 480
AGGEKSEDEA DTVGCCSLRY EHVTLKPPNT VIFDFLGKDS IRFYQEVEVD KQVFKNLTIF
490 500 510 520 530 540
KRPPKQPGHQ LFDRLDPSIL NKYLQNYMPG LTAKVFRTYN ASKTMQDQLD LIPNKGSVAE
550 560 570 580 590 600
KILKYNAANR TVAILCNHQR TVTKGHAQTV EKANNRIQEL EWQKIRCKRA ILQLDKDLLK
610 620 630 640 650 660
KEPKYFEEID DLTKEDEATI HKRIIDREIE KYQRKFVREN DKRKFEKEEL LPESQLKEWL
670 680 690 700 710 720
EKVDEKKQEF EKELKTGEVE LKSSWNSVEK IKAQVEKLEQ RIQTSSIQLK DKEENSQVSL
730 740 750 760
GTSKINYIDP RLSVVFCKKY DVPIEKIFTK TLREKFKWAI ESVDENWRF