P03018

PR
Gene name
uvrD (mutU, pdeB, rad, recL)
Protein name
DNA helicase II
Names
Species
Escherichia coli (strain K12)
KEGG Pathway
eco:b3813
EC number
5.6.2.4: Enzymes altering nucleic acid conformation
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

620-693 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

8 structures for P03018

Entry ID Method Resolution Chain Position Source
2IS1 X-ray 290 A A/B 1-680 PDB
2IS2 X-ray 300 A A/B 1-680 PDB
2IS4 X-ray 260 A A/B 1-680 PDB
2IS6 X-ray 220 A A/B 1-680 PDB
3LFU X-ray 180 A A 1-647 PDB
6YI2 NMR - A 645-720 PDB
7EGS X-ray 170 A B 654-720 PDB
AF-P03018-F1 Predicted AlphaFoldDB

No variants for P03018

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P03018

No associated diseases with P03018

2 regional properties for P03018

Type Name Position InterPro Accession
domain UvrD-like helicase, ATP-binding domain 8 - 286 IPR014016
domain UvrD-like DNA helicase, C-terminal 277 - 616 IPR014017

Functions

Description
EC Number 5.6.2.4 Enzymes altering nucleic acid conformation
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
DNA helicase complex A protein complex that possesses DNA helicase activity.
single-stranded DNA-dependent ATP-dependent DNA helicase complex A protein complex that possesses single-stranded DNA-dependent DNA helicase activity.

7 GO annotations of molecular function

Name Definition
3'-5' DNA helicase activity Unwinding a DNA helix in the direction 5' to 3', driven by ATP hydrolysis.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA helicase activity Unwinding of a DNA helix, driven by ATP hydrolysis.
DNA translocase activity Generation of movement along a single- or double-stranded DNA molecule, driven by ATP hydrolysis.
single-stranded DNA helicase activity Catalysis of the reaction: ATP + H2O = ADP + phosphate, in the presence of single-stranded DNA; drives the unwinding of a DNA helix.

11 GO annotations of biological process

Name Definition
DNA duplex unwinding The process in which interchain hydrogen bonds between two strands of DNA are broken or 'melted', generating a region of unpaired single strands.
DNA unwinding involved in DNA replication The process in which interchain hydrogen bonds between two strands of DNA are broken or 'melted', generating unpaired template strands for DNA replication.
mismatch repair A system for the correction of errors in which an incorrect base, which cannot form hydrogen bonds with the corresponding base in the parent strand, is incorporated into the daughter strand. The mismatch repair system promotes genomic fidelity by repairing base-base mismatches, insertion-deletion loops and heterologies generated during DNA replication and recombination.
mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication A mismatch repair process that corrects errors introduced that ensures the accuracy of DNA replication.
nucleotide-excision repair A DNA repair process in which a small region of the strand surrounding the damage is removed from the DNA helix as an oligonucleotide. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. Nucleotide excision repair recognizes a wide range of substrates, including damage caused by UV irradiation (pyrimidine dimers and 6-4 photoproducts) and chemicals (intrastrand cross-links and bulky adducts).
nucleotide-excision repair, DNA duplex unwinding The unwinding, or local denaturation, of the DNA duplex to create a bubble around the site of the DNA damage.
recombinational repair A DNA repair process that involves the exchange, reciprocal or nonreciprocal, of genetic material between the broken DNA molecule and a homologous DNA region.
replication fork processing The process in which a DNA replication fork that has stalled is restored to a functional state and replication is restarted. The stalling may be due to DNA damage, DNA secondary structure, bound proteins, dNTP shortage, or other causes.
response to radiation Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an electromagnetic radiation stimulus. Electromagnetic radiation is a propagating wave in space with electric and magnetic components. These components oscillate at right angles to each other and to the direction of propagation.
rolling circle DNA replication A DNA-dependent DNA replication process in which a single-stranded DNA molecule is synthesized from a circular duplex template. Replication typically does not cease when one circumference has been replicated, but continues around the circumference several more times, producing a long single strand comprising multimers of the replicon.
SOS response An error-prone process for repairing damaged microbial DNA.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q8NFZ0 FBH1 F-box DNA helicase 1 Homo sapiens (Human) PR
Q8K2I9 Fbh1 F-box DNA helicase 1 Mus musculus (Mouse) PR
10 20 30 40 50 60
MDVSYLLDSL NDKQREAVAA PRSNLLVLAG AGSGKTRVLV HRIAWLMSVE NCSPYSIMAV
70 80 90 100 110 120
TFTNKAAAEM RHRIGQLMGT SQGGMWVGTF HGLAHRLLRA HHMDANLPQD FQILDSEDQL
130 140 150 160 170 180
RLLKRLIKAM NLDEKQWPPR QAMWYINSQK DEGLRPHHIQ SYGNPVEQTW QKVYQAYQEA
190 200 210 220 230 240
CDRAGLVDFA ELLLRAHELW LNKPHILQHY RERFTNILVD EFQDTNNIQY AWIRLLAGDT
250 260 270 280 290 300
GKVMIVGDDD QSIYGWRGAQ VENIQRFLND FPGAETIRLE QNYRSTSNIL SAANALIENN
310 320 330 340 350 360
NGRLGKKLWT DGADGEPISL YCAFNELDEA RFVVNRIKTW QDNGGALAEC AILYRSNAQS
370 380 390 400 410 420
RVLEEALLQA SMPYRIYGGM RFFERQEIKD ALSYLRLIAN RNDDAAFERV VNTPTRGIGD
430 440 450 460 470 480
RTLDVVRQTS RDRQLTLWQA CRELLQEKAL AGRAASALQR FMELIDALAQ ETADMPLHVQ
490 500 510 520 530 540
TDRVIKDSGL RTMYEQEKGE KGQTRIENLE ELVTATRQFS YNEEDEDLMP LQAFLSHAAL
550 560 570 580 590 600
EAGEGQADTW QDAVQLMTLH SAKGLEFPQV FIVGMEEGMF PSQMSLDEGG RLEEERRLAY
610 620 630 640 650 660
VGVTRAMQKL TLTYAETRRL YGKEVYHRPS RFIGELPEEC VEEVRLRATV SRPVSHQRMG
670 680 690 700 710
TPMVENDSGY KLGQRVRHAK FGEGTIVNME GSGEHSRLQV AFQGQGIKWL VAAYARLESV