AiPD: Autoinhibited Protein Database

O95847

Gene name	SLC25A27 (UCP4, UNQ772/PRO1566)
Protein name	Mitochondrial uncoupling protein 4
Names	UCP 4, Solute carrier family 25 member 27
Species	Homo sapiens (Human)
KEGG Pathway	hsa:9481
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

224-293 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

224-293 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

1 structures for O95847

Entry ID	Method	Resolution	Chain	Position	Source
AF-O95847-F1	Predicted				AlphaFoldDB

222 variants for O95847

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA3838271 rs769122059	2	S>F		No	ClinGen ExAC gnomAD
TCGA novel	3	V>A	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs770393224 CA3838274	3	V>I		No	ClinGen ExAC TOPMed gnomAD
rs770393224 CA363911462	3	V>L		No	ClinGen ExAC TOPMed gnomAD
rs773680687 CA3838275	4	P>L		No	ClinGen ExAC TOPMed
CA3838278 rs773680687	4	P>Q		No	ClinGen ExAC TOPMed
rs773680687 CA363911476	4	P>R		No	ClinGen ExAC TOPMed
CA363911473 rs1194696782	4	P>S		No	ClinGen gnomAD
rs1562031397 CA363911481	5	E>Q		No	ClinGen Ensembl
CA3838280 rs759094495	7	E>G		No	ClinGen ExAC TOPMed
CA3838281 rs767033940	9	R>S		No	ClinGen ExAC TOPMed gnomAD
CA3838282 rs752365541	10	L>F		No	ClinGen ExAC gnomAD
CA363911561 rs1254072537	11	L>F		No	ClinGen TOPMed
CA363911576 rs1184600444	12	P>L		No	ClinGen gnomAD
rs760367374 CA3838283	12	P>S		No	ClinGen ExAC gnomAD
CA3838287 rs370867753	17	W>C		No	ClinGen ESP ExAC TOPMed
rs757125990 CA3838286	17	W>R		No	ClinGen ExAC gnomAD
CA3838288 rs531521297	19	R>Q		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA363911653 rs1226077170	20	A>S		No	ClinGen TOPMed
CA363911703 rs1562031524	24	L>P		No	ClinGen Ensembl
rs1284698675 CA363911719	26	S>F		No	ClinGen gnomAD
rs747204664 CA3838291	27	G>D		No	ClinGen ExAC gnomAD
CA363911750 rs1562031584	29	A>T		No	ClinGen Ensembl
CA3838292 rs769069078	29	A>V		No	ClinGen ExAC
rs781682308 CA3838293	31	T>A		No	ClinGen ExAC gnomAD
CA3838294 rs748564213	31	T>I		No	ClinGen ExAC gnomAD
CA363911807 rs748564213	31	T>N		No	ClinGen ExAC gnomAD
rs748564213 CA3838295	31	T>S		No	ClinGen ExAC gnomAD
CA363911844 rs1461516644	34	E>K		No	ClinGen TOPMed gnomAD
TCGA novel	36	A>P	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs765052707 CA3838321	37	T>A		No	ClinGen ExAC
rs772948449 COSM1079874 CA3838322	46	R>*	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ExAC NCI-TCGA gnomAD
CA363912787 rs1304344123	46	R>P		No	ClinGen TOPMed gnomAD
rs1304344123 CA363912785	46	R>Q		No	ClinGen TOPMed gnomAD
rs766435918 CA3838324	50	Q>H		No	ClinGen ExAC gnomAD
rs762937474 CA3838323	50	Q>K		No	ClinGen ExAC TOPMed gnomAD
CA363912901 rs751626969	53	A>G		No	ClinGen ExAC TOPMed gnomAD
CA3838325 rs751626969	53	A>V		No	ClinGen ExAC TOPMed gnomAD
CA3838327 rs767795442	54	A>G		No	ClinGen ExAC gnomAD
rs200951505 CA363912945	57	R>G		No	ClinGen ESP ExAC TOPMed gnomAD
rs374619315 CA3838329	57	R>P		No	ClinGen ESP ExAC TOPMed gnomAD
CA363912950 rs374619315	57	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
CA3838328 rs200951505	57	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
CA137863270 rs766815037	58	L>S		No	ClinGen Ensembl
rs1182235291 CA363912973	59	G>R		No	ClinGen TOPMed gnomAD
TCGA novel	60	D>H	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA363912998 rs1162916641	61	G>D		No	ClinGen gnomAD
CA363912993 rs1422665735	61	G>S		No	ClinGen TOPMed gnomAD
CA363912997 rs1162916641	61	G>V		No	ClinGen gnomAD
rs1366130922 CA363913015	63	R>G		No	ClinGen gnomAD
rs757702440 CA3838332	63	R>K		No	ClinGen ExAC gnomAD
rs1047974095 CA137863279	64	E>G		No	ClinGen TOPMed
CA363913062 rs1395257574	66	A>S		No	ClinGen gnomAD
rs746482027 CA3838334	67	P>H		No	ClinGen ExAC gnomAD
rs746482027 CA3838335	67	P>L		No	ClinGen ExAC gnomAD
CA363913093 rs1308118475	68	Y>S		No	ClinGen TOPMed gnomAD
rs942044441 CA137863315	69	R>S		No	ClinGen TOPMed gnomAD
TCGA novel	70	G>E	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA137863319 rs1039063818	70	G>V		No	ClinGen TOPMed
CA363913174 rs1314889719	71	M>R		No	ClinGen gnomAD
rs1582495129 CA363913196	72	V>G		No	ClinGen Ensembl
rs200548509 CA3838338	73	R>C		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs376251804 CA3838339	73	R>H	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ESP ExAC NCI-TCGA TOPMed gnomAD
CA3838340 rs376251804	73	R>L		No	ClinGen ESP ExAC TOPMed gnomAD
rs551058229 CA3838341	75	A>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs372535065 CA3838344	77	G>E		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
TCGA novel	78	I>M	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs946951680 CA137863359	79	I>T		No	ClinGen Ensembl
rs760935283 CA3838346	81	E>K		No	ClinGen ExAC TOPMed gnomAD
rs1010046655 CA137863373	83	G>D		No	ClinGen TOPMed gnomAD
rs1425507733 CA363913382	84	F>L		No	ClinGen gnomAD
CA363913529 rs1372040262	90	G>E		No	ClinGen gnomAD
CA363913539 rs1021573623	91	V>L		No	ClinGen TOPMed gnomAD
CA137863379 rs1021573623	91	V>M		No	ClinGen TOPMed gnomAD
rs1403453327 CA363913588	92	T>I		No	ClinGen TOPMed gnomAD
rs1403453327 CA363913585	92	T>R		No	ClinGen TOPMed gnomAD
rs779343845 CA3838350	94	A>S		No	ClinGen ExAC TOPMed gnomAD
rs779343845 CA3838351 COSM255356	94	A>T	central_nervous_system [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
CA363913661 rs1207300326	96	Y>C		No	ClinGen TOPMed gnomAD
rs1233931896 CA363913695	97	R>S		No	ClinGen gnomAD
rs148753471 CA3838353	99	V>I		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs148753471 CA363913731	99	V>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs751992628 CA3838389	105	R>*		No	ClinGen ExAC TOPMed gnomAD
CA3838390 rs769910678	105	R>Q		No	ClinGen ExAC gnomAD
rs1456023834 CA363914664	106	M>I		No	ClinGen TOPMed
TCGA novel	106	M>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1237046607 CA363914668	107	V>I		No	ClinGen TOPMed
rs1582499152 CA363914708	110	E>D		No	ClinGen Ensembl
CA3838392 rs753453363	113	R>*		No	ClinGen ExAC gnomAD
CA363914733 rs1334475004	113	R>Q		No	ClinGen gnomAD
rs1439488999 CA363914743	114	E>G		No	ClinGen TOPMed
rs756910447 CA3838393	115	V>A		No	ClinGen ExAC TOPMed gnomAD
CA363914754 rs1276468850	115	V>F		No	ClinGen TOPMed
CA3838395 rs143166898	120	S>N		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA363915131 rs1336945980	121	E>*		No	ClinGen gnomAD
CA3838396 rs758149357	123	E>K		No	ClinGen ExAC gnomAD
rs1258222859 CA363915167	124	H>Y		No	ClinGen gnomAD
rs1458838300 CA363915195	126	P>S		No	ClinGen gnomAD
CA3838414 rs750020901	132	I>F		No	ClinGen ExAC TOPMed gnomAD
CA3838417 rs746835187	135	M>L		No	ClinGen ExAC gnomAD
TCGA novel	135	M>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA363915577 rs1292709126	136	M>T		No	ClinGen TOPMed
CA3838419 rs781141249	138	G>C		No	ClinGen ExAC gnomAD
CA137868463 rs964182530	139	V>A		No	ClinGen gnomAD
CA3838423 rs370462167	143	F>I		No	ClinGen ESP ExAC TOPMed gnomAD
CA3838422 rs370462167	143	F>L		No	ClinGen ESP ExAC TOPMed gnomAD
CA363915693 rs370462167	143	F>V		No	ClinGen ESP ExAC TOPMed gnomAD
TCGA novel	144	L>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
VAR_069101 CA137868520 rs17853162	145	A>V		No	ClinGen UniProt Ensembl dbSNP
TCGA novel	147	P>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs771239382 CA3838424	148	T>A	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
rs774517838 CA363915819	150	L>V		No	ClinGen ExAC gnomAD
CA363915834 rs1349127498	151	V>L		No	ClinGen gnomAD
CA363915868 rs1582503352	153	V>G		No	ClinGen Ensembl
CA363915901 rs1216609199	155	M>I		No	ClinGen gnomAD
TCGA novel	156	Q>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA363915939 rs1286805802	157	M>T	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA gnomAD
TCGA novel	158	E>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA137868545 rs539097130	159	G>R		No	ClinGen 1000Genomes gnomAD
rs374823328 CA3838427	159	G>V		No	ClinGen ESP ExAC TOPMed gnomAD
CA137868566 rs377336270	162	K>E		No	ClinGen ESP TOPMed
rs371275152 CA3838429	164	E>D		No	ClinGen ESP ExAC TOPMed gnomAD
rs1245647026 CA363916112	168	L>S		No	ClinGen gnomAD
rs867117424 CA137868617	169	R>*	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed gnomAD
rs201563800 CA3838431	169	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
rs1332483378 CA363916771	170	F>V		No	ClinGen gnomAD
rs1379731591 COSM1079876 CA363916790	171	R>C	Variant assessed as Somatic; 0.0 impact. large_intestine endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated NCI-TCGA gnomAD
rs979488993 CA137870655	171	R>H		No	ClinGen TOPMed gnomAD
rs769299739 CA3838448	173	V>I		No	ClinGen ExAC gnomAD
rs1247091774 CA363916847	174	H>Q		No	ClinGen gnomAD
CA3838449 rs201819031	174	H>Y		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs762554527 CA3838450	175	H>D		No	ClinGen ExAC gnomAD
CA137870696 rs925010869	175	H>Q		No	ClinGen TOPMed gnomAD
rs765896195 CA3838452	176	A>G		No	ClinGen ExAC gnomAD
CA363916895 rs1199016294	176	A>T		No	ClinGen TOPMed gnomAD
rs765896195 CA3838451	176	A>V		No	ClinGen ExAC gnomAD
CA137870701 rs374745926	177	F>C		No	ClinGen ESP
CA3838453 rs759223042	180	I>L		No	ClinGen ExAC gnomAD
rs753660514 CA3838454	187	R>Q		No	ClinGen ExAC TOPMed gnomAD
rs1424970754 CA363917101	189	L>F		No	ClinGen gnomAD
rs1312325040 CA363917139	192	G>R		No	ClinGen TOPMed
CA137870723 rs932378479	193	W>C		No	ClinGen TOPMed gnomAD
CA3838456 rs755992198	194	V>I		No	ClinGen ExAC TOPMed gnomAD
CA3838457 rs777773875	196	N>S		No	ClinGen ExAC TOPMed gnomAD
CA363917177 rs777773875	196	N>T		No	ClinGen ExAC TOPMed gnomAD
VAR_050137 CA3838458 rs35884480	197	I>T		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
rs1402704928 CA363917212	199	R>T		No	ClinGen TOPMed
rs757382672 CA3838459	200	A>S		No	ClinGen ExAC gnomAD
rs778910615 CA3838460	204	N>T		No	ClinGen ExAC gnomAD
CA3838473 rs760496531	207	D>G		No	ClinGen ExAC gnomAD
CA363917295 rs1163077003	207	D>N		No	ClinGen TOPMed
CA363918057 rs1284667997	208	L>V		No	ClinGen gnomAD
rs552566509 CA363918132	211	Y>*		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs201797981 CA137874303	211	Y>F		No	ClinGen TOPMed gnomAD
rs1200940333 CA363918216	215	K>T		No	ClinGen gnomAD
CA363918293 rs758626551	218	L>F		No	ClinGen ExAC gnomAD
rs1193026813 CA363918283	218	L>S		No	ClinGen TOPMed
CA363918326 rs1420337839	220	L>F		No	ClinGen gnomAD
CA363918329 rs1554171387	221	N>H		No	ClinGen Ensembl
CA363918364 rs1472227791	223	P>S		No	ClinGen gnomAD
rs887728328 CA137874340	226	D>E		No	ClinGen TOPMed
CA363918493 rs1264252547	227	N>S		No	ClinGen TOPMed
TCGA novel	228	I>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1300136980 CA363918548	229	M>L		No	ClinGen gnomAD
CA3838480 rs369700969	230	T>I		No	ClinGen ESP ExAC TOPMed gnomAD
rs1293890225 CA363918645	232	G>S		No	ClinGen gnomAD
CA3838482 rs755345912	235	S>G		No	ClinGen ExAC TOPMed gnomAD
rs1024181965 CA137875611	237	C>S		No	ClinGen TOPMed
rs1472661575 CA363919435	244	I>L		No	ClinGen TOPMed
rs756582016 CA3838512	245	L>R		No	ClinGen ExAC gnomAD
CA3838513 rs778286962	246	G>E		No	ClinGen ExAC
CA137875627 rs909522823	247	T>I		No	ClinGen Ensembl
CA3838514 rs745336518	247	T>P		No	ClinGen ExAC gnomAD
CA3838516 rs779582453	249	A>T		No	ClinGen ExAC gnomAD
rs1257618943 CA363919471	249	A>V		No	ClinGen Ensembl
rs1332037494 CA363919476	250	D>H		No	ClinGen TOPMed gnomAD
rs1332037494 CA363919474	250	D>N		No	ClinGen TOPMed gnomAD
CA137875643 rs544957391	253	K>E		No	ClinGen TOPMed gnomAD
CA3838518 rs768252601	253	K>R		No	ClinGen ExAC TOPMed gnomAD
TCGA novel	254	S>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA363919583 rs1318965861	257	M>I		No	ClinGen gnomAD
rs1257805652 CA363919569	257	M>V		No	ClinGen gnomAD
CA363919624 rs1197930126	260	P>L		No	ClinGen gnomAD
CA137875663 rs978490791	260	P>S		No	ClinGen TOPMed
CA3838519 rs776429763	261	R>*	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
rs761543915 CA3838520	261	R>Q	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
rs1196436598 CA363919707	267	G>E		No	ClinGen TOPMed gnomAD
CA3838530 rs184071945	267	G>R		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
TCGA novel	269	L>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA137876618 rs763226129	270	Y>C		No	ClinGen Ensembl
CA3838532 rs373510141	273	S>L		No	ClinGen ESP TOPMed
rs983413284 CA137876670	276	C>R		No	ClinGen Ensembl
CA137876677 rs907882276	276	C>Y		No	ClinGen gnomAD
CA363919770 rs1418516819	277	L>S		No	ClinGen gnomAD
CA137876684 rs867816863	280	A>D		No	ClinGen Ensembl
CA3838537 rs754314561	281	V>F		No	ClinGen ExAC gnomAD
CA3838538 rs754314561	281	V>I		No	ClinGen ExAC gnomAD
CA363919799 rs1582514793	282	Q>E		No	ClinGen Ensembl
CA3838539 rs200844401	283	G>D		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1037761108 CA137876699	286	F>L		No	ClinGen TOPMed
rs768313753 CA3838541	287	M>I		No	ClinGen ExAC TOPMed gnomAD
CA3838540 rs746515242	287	M>V		No	ClinGen ExAC
rs1234428333 CA566914904	290	Y>*		No	ClinGen gnomAD
rs1252416184 CA363919882	293	F>L		No	ClinGen TOPMed
rs1399242074 CA363919876	293	F>L		No	ClinGen gnomAD
rs1433186199 CA363919883	294	L>I		No	ClinGen Ensembl
CA3838543 rs747883074	295	P>S		No	ClinGen ExAC gnomAD
CA363919896 rs1239495723	296	S>P		No	ClinGen TOPMed gnomAD
rs751030565 CA3838561	305	M>V		No	ClinGen ExAC gnomAD
rs780793044 CA3838563	306	V>A		No	ClinGen ExAC gnomAD
rs754424020 CA3838562	306	V>M		No	ClinGen ExAC gnomAD
rs1421502781 CA363920898	307	F>C		No	ClinGen gnomAD
CA363920944 rs1377462927	308	W>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
rs747749538 CA3838564	310	T>I		No	ClinGen ExAC gnomAD
rs747749538 CA363920997	310	T>N		No	ClinGen ExAC gnomAD
TCGA novel	315	R>G	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs755885581 CA3838565	317	M>T		No	ClinGen ExAC gnomAD
rs1309080664 CA363921256	318	S>N		No	ClinGen TOPMed
rs749096343 CA3838567	320	V>G		No	ClinGen ExAC gnomAD
CA363921312 rs1562044730	320	V>I		No	ClinGen Ensembl
CA363921391 rs1376202718	322	P>L		No	ClinGen gnomAD
rs1299175117 CA363921371	322	P>S	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA gnomAD

No associated diseases with O95847

3 regional properties for O95847

Type	Name	Position	InterPro Accession
repeat	Mitochondrial substrate/solute carrier	20 - 118	IPR018108-1
repeat	Mitochondrial substrate/solute carrier	125 - 219	IPR018108-2
repeat	Mitochondrial substrate/solute carrier	226 - 317	IPR018108-3

Functions

		Description
EC Number
Subcellular Localization	Mitochondrion inner membrane ; Multi-pass membrane protein	Localizes to neuronal cell body and processes Within mitochondrial inner membrane, it is mainly observed in the inner boundary membrane locally separated from F(1)F(0) ATP synthase, which is preferentially localized in cristae
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

4 GO annotations of cellular component

Name	Definition
integral component of membrane	The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
mitochondrial inner membrane	The inner, i.e. lumen-facing, lipid bilayer of the mitochondrial envelope. It is highly folded to form cristae.
mitochondrial membrane	Either of the lipid bilayers that surround the mitochondrion and form the mitochondrial envelope.
mitochondrion	A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

1 GO annotations of molecular function

Name	Definition
transmembrane transporter activity	Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.

1 GO annotations of biological process

Name	Definition
response to cold	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cold stimulus, a temperature stimulus below the optimal temperature for that organism.

1 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q9XI74	PUMP3	Mitochondrial uncoupling protein 3	Arabidopsis thaliana (Mouse-ear cress)	PR

10	20	30	40	50	60
MSVPEEEERL	LPLTQRWPRA	SKFLLSGCAA	TVAELATFPL	DLTKTRLQMQ	GEAALARLGD
70	80	90	100	110	120
GARESAPYRG	MVRTALGIIE	EEGFLKLWQG	VTPAIYRHVV	YSGGRMVTYE	HLREVVFGKS
130	140	150	160	170	180
EDEHYPLWKS	VIGGMMAGVI	GQFLANPTDL	VKVQMQMEGK	RKLEGKPLRF	RGVHHAFAKI
190	200	210	220	230	240
LAEGGIRGLW	AGWVPNIQRA	ALVNMGDLTT	YDTVKHYLVL	NTPLEDNIMT	HGLSSLCSGL
250	260	270	280	290	300
VASILGTPAD	VIKSRIMNQP	RDKQGRGLLY	KSSTDCLIQA	VQGEGFMSLY	KGFLPSWLRM
310	320
TPWSMVFWLT	YEKIREMSGV	SPF