O62305

EV
Gene name
unc-43 (K11E8.1)
Protein name
Calcium/calmodulin-dependent protein kinase type II
Names
CaM kinase II, Uncoordinated protein 43
Species
Caenorhabditis elegans
KEGG Pathway
EC number
2.7.11.17: Protein-serine/threonine kinases
Protein Class
SERINE/THREONINE-PROTEIN KINASE (PTHR24347)

Descriptions

CaMKII is one of the most important transducers of Ca2+signals in a variety of cell types and is highly conserved across animal species. The calmodulin-responsive regulatory segment forms a coiled coil structure that blocks peptide and ATP binding to the otherwise intrinsically active kinase domain.Ca2+/CaM removes the autoinhibitory regulatory segment located C-terminal to the kinase domain which releases the catalytic activity of the enzyme.

Autoinhibitory domains (AIDs)

273-317 (Regulatory segment) EV

Target domain

12-269 (Protein kinase domain)

Relief mechanism

Partner binding

Assay

Structural analysis

Accessory elements

154-176 (Activation loop from InterPro)

Target domain

12-269 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

6 structures for O62305

Entry ID Method Resolution Chain Position Source
2BDW X-ray 180 A PDB
2F86 X-ray 264 A B/D/F/H/J/L/N 585-720 PDB
3KK8 X-ray 172 A A 5-288 PDB
3KK9 X-ray 321 A A 6-287 PDB
3KL8 X-ray 337 A A/C/E/G/I 5-273 PDB
AF-O62305-F1 Predicted AlphaFoldDB

No variants for O62305

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O62305

No associated diseases with O62305

3 regional properties for O62305

Type Name Position InterPro Accession
domain Basic-leucine zipper domain 71 - 135 IPR004827
domain Vertebrate interleukin-3 regulated transcription factor 130 - 461 IPR010533
domain Nuclear factor interleukin-3-regulated protein-like, bZIP domain 72 - 131 IPR047106

Functions

Description
EC Number 2.7.11.17 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cell projection, axon
  • Perikaryon
  • Localizes at or near the Golgi apparatus (PubMed:16079277)
  • Localizes to post-synaptic regions and is enriched in punctate structures in axons of AWC neurons where it co-localizes with tir-1
  • Localization is regulated by tir-1 (PubMed:15625192)
PANTHER Family PTHR24347 SERINE/THREONINE-PROTEIN KINASE
PANTHER Subfamily PTHR24347:SF441 CALCIUM_CALMODULIN-DEPENDENT PROTEIN KINASE TYPE II SUBUNIT GAMMA
PANTHER Protein Class non-receptor serine/threonine protein kinase
protein modifying enzyme
PANTHER Pathway Category Inflammation mediated by chemokine and cytokine signaling pathway
CaMK
Ionotropic glutamate receptor pathway
CaMKII

4 GO annotations of cellular component

Name Definition
axon cytoplasm Any cytoplasm that is part of a axon.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
neuron projection A prolongation or process extending from a nerve cell, e.g. an axon or dendrite.
perikaryon The portion of the cell soma (neuronal cell body) that excludes the nucleus.

8 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
calmodulin-dependent protein kinase activity Calmodulin-dependent catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; and ATP + a protein threonine = ADP + protein threonine phosphate.
identical protein binding Binding to an identical protein or proteins.
metal ion binding Binding to a metal ion.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.
transmembrane transporter binding Binding to a transmembrane transporter, a protein or protein complex that enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.

7 GO annotations of biological process

Name Definition
MAPK cascade An intracellular protein kinase cascade containing at least a MAPK, a MAPKK and a MAP3K. The cascade can also contain an additional tiers: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinase in the downstream tier to transmit a signal within a cell.
medium-term memory The memory process that deals with the storage, retrieval and modification of information received at a time ago that is intermediate between that of short and long term memory (30min - 7hrs in Drosophila melanogaster).
peptidyl-serine phosphorylation The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
peptidyl-threonine phosphorylation The phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
positive regulation of gene expression Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
protein phosphorylation The process of introducing a phosphate group on to a protein.
serotonin biosynthetic process The chemical reactions and pathways resulting in the formation of serotonin (5-hydroxytryptamine), a monoamine neurotransmitter occurring in the peripheral and central nervous systems, also having hormonal properties.

24 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2HJF7 CAMK2D Calcium/calmodulin-dependent protein kinase type II subunit delta Bos taurus (Bovine) SS
Q3MHJ9 CAMK2B Calcium/calmodulin-dependent protein kinase type II subunit beta Bos taurus (Bovine) SS
Q5ZKI0 CAMK2D Calcium/calmodulin-dependent protein kinase type II delta chain Gallus gallus (Chicken) SS
Q24210 CASK Peripheral plasma membrane protein CASK Drosophila melanogaster (Fruit fly) SS
Q00168 CaMKII Calcium/calmodulin-dependent protein kinase type II alpha chain Drosophila melanogaster (Fruit fly) SS
O14936 CASK Peripheral plasma membrane protein CASK Homo sapiens (Human) EV
Q13554 CAMK2B Calcium/calmodulin-dependent protein kinase type II subunit beta Homo sapiens (Human) EV
Q13555 CAMK2G Calcium/calmodulin-dependent protein kinase type II subunit gamma Homo sapiens (Human) EV
Q9UQM7 CAMK2A Calcium/calmodulin-dependent protein kinase type II subunit alpha Homo sapiens (Human) EV
Q13557 CAMK2D Calcium/calmodulin-dependent protein kinase type II subunit delta Homo sapiens (Human) EV
O70589 Cask Peripheral plasma membrane protein CASK Mus musculus (Mouse) SS
P28652 Camk2b Calcium/calmodulin-dependent protein kinase type II subunit beta Mus musculus (Mouse) SS
Q923T9 Camk2g Calcium/calmodulin-dependent protein kinase type II subunit gamma Mus musculus (Mouse) SS
P11798 Camk2a Calcium/calmodulin-dependent protein kinase type II subunit alpha Mus musculus (Mouse) PR
Q6PHZ2 Camk2d Calcium/calmodulin-dependent protein kinase type II subunit delta Mus musculus (Mouse) SS
Q95266 CAMK2D Calcium/calmodulin-dependent protein kinase type II subunit delta Sus scrofa (Pig) SS
P08413 Camk2b Calcium/calmodulin-dependent protein kinase type II subunit beta Rattus norvegicus (Rat) SS
P11730 Camk2g Calcium/calmodulin-dependent protein kinase type II subunit gamma Rattus norvegicus (Rat) SS
P11275 Camk2a Calcium/calmodulin-dependent protein kinase type II subunit alpha Rattus norvegicus (Rat) PR
P15791 Camk2d Calcium/calmodulin-dependent protein kinase type II subunit delta Rattus norvegicus (Rat) SS
P54936 lin-2 Protein lin-2 Caenorhabditis elegans SS
Q9TXJ0 cmk-1 Calcium/calmodulin-dependent protein kinase type 1 Caenorhabditis elegans PR
Q6DEH3 camk2d1 Calcium/calmodulin-dependent protein kinase type II delta 1 chain Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q6DGS3 camk2d2 Calcium/calmodulin-dependent protein kinase type II delta 2 chain Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MMNASTKFSD NYDVKEELGK GAFSVVRRCV HKTTGLEFAA KIINTKKLSA RDFQKLEREA
70 80 90 100 110 120
RICRKLQHPN IVRLHDSIQE ESFHYLVFDL VTGGELFEDI VAREFYSEAD ASHCIQQILE
130 140 150 160 170 180
SIAYCHSNGI VHRDLKPENL LLASKAKGAA VKLADFGLAI EVNDSEAWHG FAGTPGYLSP
190 200 210 220 230 240
EVLKKDPYSK PVDIWACGVI LYILLVGYPP FWDEDQHRLY AQIKAGAYDY PSPEWDTVTP
250 260 270 280 290 300
EAKSLIDSML TVNPKKRITA DQALKVPWIC NRERVASAIH RQDTVDCLKK FNARRKLKAA
310 320 330 340 350 360
ISAVKMVTRM SGVLRTSDST GSVASNGSTT HDASQVAGTS SQPTSPAAEV YPNVLLFNPQ
370 380 390 400 410 420
KFPRNCVHPF TTHPYYSPKE SSKKKLFFTL LFEVCPHTSR SHILLRDNTK NIYHPYHCFT
430 440 450 460 470 480
NKMSNYERAA PSSHGSSTTK KIANAIADLV IRRSSPSIRR KTEADVHNSN RNRKVSAPAN
490 500 510 520 530 540
LQHALVPVID VVVATGALAS SSVDNLSAST SSDLGRNLLN KKEQGPPSTI KESSESSQTI
550 560 570 580 590 600
DDNDSEKGGG QLKHENTVVR ADGATGIVSS SNSSTASKSS STNLSAQKQD IVRVTQTLLD
610 620 630 640 650 660
AISCKDFETY TRLCDTSMTC FEPEALGNLI EGIEFHRFYF DGNRKNQVHT TMLNPNVHII
670 680 690 700 710
GEDAACVAYV KLTQFLDRNG EAHTRQSQES RVWSKKQGRW VCVHVHRSTQ PSTNTTVSEF