O35980
PR
Gene name |
Nthl1 (Nth1) |
Protein name |
Endonuclease III-like protein 1 |
Names |
Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase, DNA glycosylase/AP lyase |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:18207 |
EC number |
4.2.99.18: Other carbon-oxygen lyases |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for O35980
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-O35980-F1 | Predicted | AlphaFoldDB |
19 variants for O35980
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs3389444701 | 18 | I>V | No | EVA | |
| rs3389420211 | 21 | E>* | No | EVA | |
| rs864258469 | 35 | E>A | No | EVA | |
| rs233047438 | 43 | V>M | No | EVA | |
| rs250656719 | 48 | R>K | No | EVA | |
| rs3389439726 | 49 | T>P | No | EVA | |
| rs47475127 | 52 | T>M | No | EVA | |
| rs46089833 | 112 | S>P | No | EVA | |
| rs3389434074 | 128 | S>I | No | EVA | |
| rs3389420169 | 145 | R>P | No | EVA | |
| rs3389402009 | 150 | E>D | No | EVA | |
| rs241042012 | 182 | A>T | No | EVA | |
| rs258523289 | 197 | A>T | No | EVA | |
| rs3389389068 | 226 | V>M | No | EVA | |
| rs3389420136 | 228 | T>I | No | EVA | |
| rs3389450996 | 229 | H>L | No | EVA | |
| rs226439237 | 243 | M>V | No | EVA | |
| rs213256357 | 292 | A>T | No | EVA | |
| rs3389409267 | 293 | L>Q | No | EVA |
No associated diseases with O35980
4 regional properties for O35980
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| conserved_site | Helix-hairpin-helix motif | 187 - 211 | IPR000445 |
| domain | HhH-GPD domain | 118 - 276 | IPR003265 |
| conserved_site | Endonuclease III-like, iron-sulphur cluster loop motif | 277 - 297 | IPR003651 |
| conserved_site | Endonuclease III-like, conserved site-2 | 190 - 219 | IPR004036 |
Functions
| Description | ||
|---|---|---|
| EC Number | 4.2.99.18 | Other carbon-oxygen lyases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
2 GO annotations of cellular component
| Name | Definition |
|---|---|
| mitochondrion | A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
8 GO annotations of molecular function
| Name | Definition |
|---|---|
| 4 iron, 4 sulfur cluster binding | Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. |
| class I DNA-(apurinic or apyrimidinic site) endonuclease activity | Catalysis of the cleavage of an AP site 3' of the baseless site by a beta-lyase mechanism, leaving an unsaturated aldehyde, termed a 3'-(4-hydroxy-5-phospho-2-pentenal) residue, and a 5'-phosphate. |
| damaged DNA binding | Binding to damaged DNA. |
| DNA N-glycosylase activity | Catalysis of the removal of damaged bases by cleaving the N-C1' glycosidic bond between the target damaged DNA base and the deoxyribose sugar. The reaction releases a free base and leaves an apurinic/apyrimidinic (AP) site. |
| DNA-(apurinic or apyrimidinic site) endonuclease activity | Catalysis of the cleavage of the C-O-P bond in the AP site created when DNA glycosylase removes a damaged base, involved in the DNA base excision repair pathway (BER). |
| double-stranded DNA binding | Binding to double-stranded DNA. |
| metal ion binding | Binding to a metal ion. |
| oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity | Catalysis of the removal oxidized pyrimidine bases by cleaving the N-C1' glycosidic bond between the oxidized pyrimidine and the deoxyribose sugar. The reaction involves formation of a covalent enzyme-pyrimidine base intermediate. Release of the enzyme and free base by a beta-elimination or a beta, gamma-elimination mechanism results in the cleavage of the DNA backbone 3' of the apyrimidinic (AP) site. |
4 GO annotations of biological process
| Name | Definition |
|---|---|
| base-excision repair, AP site formation | The formation of an AP site, a deoxyribose sugar with a missing base, by DNA glycosylase which recognizes an altered base in DNA and catalyzes its hydrolytic removal. This sugar phosphate is the substrate recognized by the AP endonuclease, which cuts the DNA phosphodiester backbone at the 5' side of the altered site to leave a gap which is subsequently repaired. |
| DNA repair | The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway. |
| nucleotide-excision repair | A DNA repair process in which a small region of the strand surrounding the damage is removed from the DNA helix as an oligonucleotide. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. Nucleotide excision repair recognizes a wide range of substrates, including damage caused by UV irradiation (pyrimidine dimers and 6-4 photoproducts) and chemicals (intrastrand cross-links and bulky adducts). |
| nucleotide-excision repair, DNA incision, 5'-to lesion | The endonucleolytic cleavage of the damaged strand of DNA 5' to the site of damage. The incision occurs at the junction of single-stranded DNA and double-stranded DNA that is formed when the DNA duplex is unwound. The incision follows the incision formed 3' to the site of damage. |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MNSGVRMVTR | SRSRATRIAS | EGCREELAPR | EAAAEGRKSH | RPVRHPRRTQ | KTHVAYEAAN |
| 70 | 80 | 90 | 100 | 110 | 120 |
| GEEGEDAEPL | KVPVWEPQNW | QQQLANIRIM | RSKKDAPVDQ | LGAEHCYDAS | ASPKVRRYQV |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LLSLMLSSQT | KDQVTAGAMQ | RLRARGLTVE | SILQTDDDTL | GRLIYPVGFW | RNKVKYIKQT |
| 190 | 200 | 210 | 220 | 230 | 240 |
| TAILQQRYEG | DIPASVAELV | ALPGVGPKMA | HLAMAVAWGT | ISGIAVDTHV | HRIANRLRWT |
| 250 | 260 | 270 | 280 | 290 | |
| KKMTKTPEET | RKNLEEWLPR | VLWSEVNGLL | VGFGQQICLP | VHPRCQACLN | KALCPAAQDL |