O35343
Gene name |
Kpna4 (Qip1) |
Protein name |
Importin subunit alpha-3 |
Names |
Importin alpha Q1 , Qip1 , Karyopherin subunit alpha-4 |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:16649 |
EC number |
|
Protein Class |
|
Descriptions
(Annotation based on sequence homology with P52293)
Kpna2 functions in nuclear protein import as an adapter protein and binds directly to substrates containing either a simple or bipartite NLS motif. Importin-alpha is shown to exist in a monomeric autoinhibited state. Association with importin-beta increases the affinity for NLSs. The truncation of N-terminal autoinhibitory region containing an importin-beta-binding domain elevated the affinity of Kpna2 to NLS peptides.
Autoinhibitory domains (AIDs)
Target domain |
137-229 (Major NLS-binding site);306-394 (Minor NLS-binding site) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Fontes MR et al. (2000) "Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha", Journal of molecular biology, 297, 1183-94
- Fontes MR et al. (2003) "Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha", The Journal of biological chemistry, 278, 27981-7
- Catimel B et al. (2001) "Biophysical characterization of interactions involving importin-alpha during nuclear import", The Journal of biological chemistry, 276, 34189-98
- Pumroy RA et al. (2015) "Molecular determinants for nuclear import of influenza A PB2 by importin α isoforms 3 and 7", Structure (London, England : 1993), 23, 374-84
- Kimoto C et al. (2015) "Functional characterization of importin α8 as a classical nuclear localization signal receptor", Biochimica et biophysica acta, 1853, 2676-83
- Miyatake H et al. (2015) "Crystal structure of human importin-α1 (Rch1), revealing a potential autoinhibition mode involving homodimerization", PloS one, 10, e0115995
- Kobe B (1999) "Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha", Nature structural biology, 6, 388-97
Autoinhibited structure
Activated structure
1 structures for O35343
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-O35343-F1 | Predicted | AlphaFoldDB |
10 variants for O35343
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs3388632173 | 62 | I>L | No | EVA | |
| rs3388632232 | 68 | V>E | No | EVA | |
| rs3388638043 | 95 | A>T | No | EVA | |
| rs3388630279 | 176 | Q>R | No | EVA | |
| rs3388632295 | 306 | R>L | No | EVA | |
| rs3388635189 | 315 | T>N | No | EVA | |
| rs3388632275 | 361 | V>I | No | EVA | |
| rs3388622633 | 503 | G>D | No | EVA | |
| rs3388634070 | 505 | T>S | No | EVA | |
| rs3393167044 | 522 | F>R | No | EVA |
No associated diseases with O35343
5 regional properties for O35343
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| male germ cell nucleus | The nucleus of a male germ cell, a reproductive cell in males. |
| NLS-dependent protein nuclear import complex | A dimer consisting of an alpha and a beta-subunit that imports proteins with an NLS into the nucleus through a nuclear pore. |
| nucleoplasm | That part of the nuclear content other than the chromosomes or the nucleolus. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| nuclear import signal receptor activity | Combining with a nuclear import signal (NIS) on a cargo to be transported, to mediate transport of the cargo through the nuclear pore, from the cytoplasm to the nuclear lumen. The cargo can be either a RNA or a protein. |
| nuclear localization sequence binding | Binding to a nuclear localization sequence, a specific peptide sequence that acts as a signal to localize the protein within the nucleus. |
3 GO annotations of biological process
| Name | Definition |
|---|---|
| NLS-bearing protein import into nucleus | The directed movement of a protein bearing a nuclear localization signal (NLS) from the cytoplasm into the nucleus, across the nuclear envelope. |
| protein import into nucleus | The directed movement of a protein from the cytoplasm to the nucleus. |
| response to hydrogen peroxide | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hydrogen peroxide (H2O2) stimulus. |
30 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q02821 | SRP1 | Importin subunit alpha | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | SS |
| Q0V7M0 | KPNA6 | Importin subunit alpha-7 | Bos taurus (Bovine) | SS |
| A2VE08 | KPNA1 | Importin subunit alpha-5 | Bos taurus (Bovine) | SS |
| C1JZ66 | KPNA7 | Importin subunit alpha-8 | Bos taurus (Bovine) | PR |
| Q5ZML1 | KPNA1 | Importin subunit alpha-5 | Gallus gallus (Chicken) | SS |
| P52295 | Pen | Importin subunit alpha | Drosophila melanogaster (Fruit fly) | SS |
| O00505 | KPNA3 | Importin subunit alpha-4 | Homo sapiens (Human) | SS |
| A9QM74 | KPNA7 | Importin subunit alpha-8 | Homo sapiens (Human) | EV |
| P52294 | KPNA1 | Importin subunit alpha-5 | Homo sapiens (Human) | SS |
| O15131 | KPNA5 | Importin subunit alpha-6 | Homo sapiens (Human) | SS |
| O60684 | KPNA6 | Importin subunit alpha-7 | Homo sapiens (Human) | SS |
| P52292 | KPNA2 | Importin subunit alpha-1 | Homo sapiens (Human) | EV |
| O00629 | KPNA4 | Importin subunit alpha-3 | Homo sapiens (Human) | SS |
| P52293 | Kpna2 | Importin subunit alpha-1 | Mus musculus (Mouse) | EV |
| O35344 | Kpna3 | Importin subunit alpha-4 | Mus musculus (Mouse) | SS |
| O35345 | Kpna6 | Importin subunit alpha-7 | Mus musculus (Mouse) | PR |
| Q60960 | Kpna1 | Importin subunit alpha-5 | Mus musculus (Mouse) | SS |
| C0LLJ0 | Kpna7 | Importin subunit alpha-8 | Mus musculus (Mouse) | SS |
| C6K7I2 | KPNA7 | Importin subunit alpha-8 | Sus scrofa (Pig) | SS |
| P83953 | Kpna1 | Importin subunit alpha-5 | Rattus norvegicus (Rat) | SS |
| Q56R16 | Kpna5 | Importin subunit alpha-6 | Rattus norvegicus (Rat) | SS |
| Q71VM4 | Os01g0253300 | Importin subunit alpha-1a | Oryza sativa subsp. japonica (Rice) | SS |
| P91276 | ima-2 | Importin subunit alpha-2 | Caenorhabditis elegans | SS |
| Q19969 | ima-3 | Importin subunit alpha-3 | Caenorhabditis elegans | SS |
| O04294 | IMPA3 | Importin subunit alpha-3 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FJ09 | IMPA5 | Importin subunit alpha-5 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9M9X7 | IMPA7 | Importin subunit alpha-7 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FWY7 | IMPA6 | Importin subunit alpha-6 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O22478 | Importin subunit alpha | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) | SS | |
| Q503E9 | kpna5 | Importin subunit alpha-6 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| METMASPGKD | NYRMKSYKNN | ALNPEEMRRR | REEEGIQLRK | QKREQQLFKR | RNVELINEEA |
| 70 | 80 | 90 | 100 | 110 | 120 |
| AMFDSLLMDS | YVSSTTGESV | ITREMVEMLF | SDDSDLQLAT | TQKFRKLLSK | EPSPPIDEVI |
| 130 | 140 | 150 | 160 | 170 | 180 |
| NTPGVVDRFV | EFLKRNENCT | LQFEAAWALT | NIASGTSQQT | KIVIEAGAVP | IFIELLNSDF |
| 190 | 200 | 210 | 220 | 230 | 240 |
| EDVQEQAVWA | LGNIAGDSSL | CRDYVLNCSI | LNPLLTLLTK | STRLTMTRNA | VWALSNLCRG |
| 250 | 260 | 270 | 280 | 290 | 300 |
| KNPPPEFAKV | SPCLPVLSRL | LFSSDSDLLA | DACWALSYLS | DGPNEKIQAV | IDSGVCRRLV |
| 310 | 320 | 330 | 340 | 350 | 360 |
| ELLMHNDYKV | ASPALRAVGN | IVTGDDIQTQ | VILNCSALPC | LLHLLSSSKE | SIRKEACWTI |
| 370 | 380 | 390 | 400 | 410 | 420 |
| SNITAGNRAQ | IQAVIDANIF | PVLIEILQKA | EFRTRKEAAW | AITNATSGGT | PEQIRYLVSL |
| 430 | 440 | 450 | 460 | 470 | 480 |
| GCIKPLCDLL | TVMDSKIVQV | ALNGLENILR | LGEQESKRSG | SGVNPYCGLI | EEAYGLDKIE |
| 490 | 500 | 510 | 520 | 530 | |
| FLQSHENQEI | YQKAFDLIEH | YFGVEDDDSS | LAPQVDETQQ | QFIFQQPEAP | MEGFQL |