Q9LF79
SS
Gene name |
ACA8 |
Protein name |
Calcium-transporting ATPase 8, plasma membrane-type |
Names |
Ca(2+)-ATPase isoform 8 |
Species |
Bos taurus (Bovine) |
KEGG Pathway |
ath:AT5G57110 |
EC number |
2.7.10.1: Protein-tyrosine kinases |
Protein Class |
|
Descriptions
The c-Kit receptor protein-tyrosine kinase is tightly regulated in normal cells, whereas deregulated c-Kit kinase activity is implicated in the pathogenesis of human cancers. Crystal structures of c-Kit kinase reveal a manner in which the small juxtamembrane domain functions to maintain the kinase in an autoinhibited state. Insertion of this autoinhibitory domain into the cleft between the kinase N- and C-lobes flips the DFG motif into its off state, thereby inducing the activation loop to fold back over from its extended conformation in the active kinase where it binds as a pseudosubstrate at the kinase-active center.
Autoinhibitory domains (AIDs)
Target domain |
132-1047 (P-type ATPase) |
Relief mechanism |
Partner binding |
Assay |
Deletion assay, Structural analysis |
Accessory elements
No accessory elements
References
- Baekgaard L et al. (2006) "The plant plasma membrane Ca2+ pump ACA8 contains overlapping as well as physically separated autoinhibitory and calmodulin-binding domains", The Journal of biological chemistry, 281, 1058-65
- Luoni L et al. (2004) "Auto-inhibition of Arabidopsis thaliana plasma membrane Ca2+-ATPase involves an interaction of the N-terminus with the small cytoplasmic loop", FEBS letters, 574, 20-4
- Tidow H et al. (2010) "Expression, purification, crystallization and preliminary X-ray analysis of calmodulin in complex with the regulatory domain of the plasma-membrane Ca2+-ATPase ACA8", Acta crystallographica. Section F, Structural biology and crystallization communications, 66, 361-3
- Tidow H et al. (2012) "A bimodular mechanism of calcium control in eukaryotes", Nature, 491, 468-72
Autoinhibited structure
Activated structure
3 structures for Q9LF79
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 2M73 | NMR | - | A | 43-67 | PDB |
| 4AQR | X-ray | 195 A | D | 40-95 | PDB |
| AF-Q9LF79-F1 | Predicted | AlphaFoldDB |
26 variants for Q9LF79
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| tmp_5_23116821_G_A | 13 | R>C | No | 1000Genomes | |
| tmp_5_23116552_C_G | 68 | K>N | No | 1000Genomes | |
| tmp_5_23115942_C_T | 175 | R>H | No | 1000Genomes | |
| ENSVATH12819766 | 183 | R>M | No | 1000Genomes | |
| tmp_5_23115551_C_G | 233 | V>L | No | 1000Genomes | |
| tmp_5_23114909_T_G | 319 | N>H | No | 1000Genomes | |
| tmp_5_23114355_A_C | 404 | N>K | No | 1000Genomes | |
| ENSVATH14636957 | 404 | N>S | No | 1000Genomes | |
| tmp_5_23113813_T_A | 481 | S>C | No | 1000Genomes | |
| tmp_5_23113812_C_G | 481 | S>T | No | 1000Genomes | |
| ENSVATH14636952 | 487 | L>I | No | 1000Genomes | |
| tmp_5_23113618_G_C | 512 | T>S | No | 1000Genomes | |
| ENSVATH12819713 | 532 | P>L | No | 1000Genomes | |
| ENSVATH12819708 | 563 | A>T | No | 1000Genomes | |
| ENSVATH07437805 | 587 | K>E | No | 1000Genomes | |
| ENSVATH07437800 | 610 | S>C | No | 1000Genomes | |
| ENSVATH14636946 | 620 | P>L | No | 1000Genomes | |
| tmp_5_23112659_G_A | 657 | P>L | No | 1000Genomes | |
| ENSVATH00738592 | 727 | A>V | No | 1000Genomes | |
| ENSVATH12819650 | 728 | D>G | No | 1000Genomes | |
| ENSVATH14636943 | 754 | K>E | No | 1000Genomes | |
| tmp_5_23111743_T_A | 776 | Q>L | No | 1000Genomes | |
| ENSVATH07437762 | 978 | P>L | No | 1000Genomes | |
| tmp_5_23110415_T_C | 981 | K>E | No | 1000Genomes | |
| ENSVATH07437759 | 1018 | T>A | No | 1000Genomes | |
| tmp_5_23109974_T_C | 1057 | K>E | No | 1000Genomes |
No associated diseases with Q9LF79
12 regional properties for Q9LF79
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 1076 - 1343 | IPR000719 |
| domain | Serine-threonine/tyrosine-protein kinase, catalytic domain | 1076 - 1334 | IPR001245 |
| domain | Sema domain | 27 - 514 | IPR001627 |
| repeat | Plexin repeat | 519 - 560 | IPR002165 |
| domain | IPT domain | 561 - 654 | IPR002909-1 |
| domain | IPT domain | 655 - 738 | IPR002909-2 |
| domain | IPT domain | 740 - 835 | IPR002909-3 |
| domain | IPT domain | 837 - 933 | IPR002909-4 |
| active_site | Tyrosine-protein kinase, active site | 1198 - 1210 | IPR008266 |
| domain | PSI domain | 518 - 561 | IPR016201 |
| binding_site | Protein kinase, ATP binding site | 1082 - 1108 | IPR017441 |
| domain | Tyrosine-protein kinase, catalytic domain | 1076 - 1335 | IPR020635 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.10.1 | Protein-tyrosine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| integral component of plasma membrane | The component of the plasma membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane. |
| intracellular membrane-bounded organelle | Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane and occurring within the cell. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| plasmodesma | A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell. |
| plastid | Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATPase-coupled cation transmembrane transporter activity | Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + cation(out) = ADP + phosphate + cation(in). |
| calmodulin binding | Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states. |
| metal ion binding | Binding to a metal ion. |
| P-type calcium transporter activity | Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + Ca2+(in) = ADP + phosphate + Ca2+(out). |
| protein self-association | Binding to a domain within the same polypeptide. |
1 GO annotations of biological process
| Name | Definition |
|---|---|
| response to nematode | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a nematode. |
30 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P38929 | PMC1 | Calcium-transporting ATPase 2 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| D3K0R6 | ATP2B4 | Plasma membrane calcium-transporting ATPase 4 | Bos taurus (Bovine) | SS |
| Q01814 | ATP2B2 | Plasma membrane calcium-transporting ATPase 2 | Homo sapiens (Human) | SS |
| P23634 | ATP2B4 | Plasma membrane calcium-transporting ATPase 4 | Homo sapiens (Human) | EV |
| P20020 | ATP2B1 | Plasma membrane calcium-transporting ATPase 1 | Homo sapiens (Human) | SS |
| Q16720 | ATP2B3 | Plasma membrane calcium-transporting ATPase 3 | Homo sapiens (Human) | EV |
| G5E829 | Atp2b1 | Plasma membrane calcium-transporting ATPase 1 | Mus musculus (Mouse) | SS |
| Q9R0K7 | Atp2b2 | Plasma membrane calcium-transporting ATPase 2 | Mus musculus (Mouse) | SS |
| Q6Q477 | Atp2b4 | Plasma membrane calcium-transporting ATPase 4 | Mus musculus (Mouse) | SS |
| P23220 | ATP2B1 | Plasma membrane calcium-transporting ATPase 1 | Sus scrofa (Pig) | SS |
| P11506 | Atp2b2 | Plasma membrane calcium-transporting ATPase 2 | Rattus norvegicus (Rat) | SS |
| Q64542 | Atp2b4 | Plasma membrane calcium-transporting ATPase 4 | Rattus norvegicus (Rat) | SS |
| P11505 | Atp2b1 | Plasma membrane calcium-transporting ATPase 1 | Rattus norvegicus (Rat) | SS |
| Q64568 | Atp2b3 | Plasma membrane calcium-transporting ATPase 3 | Rattus norvegicus (Rat) | SS |
| Q8RUN1 | ACA1 | Calcium-transporting ATPase 1, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q6ATV4 | ACA3 | Calcium-transporting ATPase 3, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q65X71 | ACA6 | Probable calcium-transporting ATPase 6, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q7XEK4 | ACA7 | Calcium-transporting ATPase 7, plasma membrane-type | Oryza sativa subsp japonica (Rice) | EV |
| Q2QY12 | ACA9 | Probable calcium-transporting ATPase 9, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q2QMX9 | ACA10 | Calcium-transporting ATPase 10, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q7X8B5 | ACA5 | Calcium-transporting ATPase 5, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| O22218 | ACA4 | Calcium-transporting ATPase 4, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O64806 | ACA7 | Putative calcium-transporting ATPase 7, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O81108 | ACA2 | Calcium-transporting ATPase 2, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | EV |
| Q9LIK7 | ACA13 | Putative calcium-transporting ATPase 13, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LU41 | ACA9 | Calcium-transporting ATPase 9, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LY77 | ACA12 | Calcium-transporting ATPase 12, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9M2L4 | ACA11 | Putative calcium-transporting ATPase 11, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9SZR1 | ACA10 | Calcium-transporting ATPase 10, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q37145 | ACA1 | Calcium-transporting ATPase 1 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MRGARGAWDF | LFVLLLLLLV | QTGSSQPSVS | PGELSLPSIH | PAKSELIVSV | GDEIRLLCTD |
| 70 | 80 | 90 | 100 | 110 | 120 |
| PGFVKWTFEI | LGQLSEKTNP | EWITEKAEAT | NTGNYTCTNK | GGLSSSIYVF | VRDPEKLFLI |
| 130 | 140 | 150 | 160 | 170 | 180 |
| DLPLYGKEEN | DTLVRCPLTD | PEVTNYSLTG | CEGKPLPKDL | TFVADPKAGI | TIRNVKREYH |
| 190 | 200 | 210 | 220 | 230 | 240 |
| RLCLHCSANQ | RGKSMLSKKF | TLKVRAAIKA | VPVVSVSKTS | YLLREGEEFA | VTCLIKDVSS |
| 250 | 260 | 270 | 280 | 290 | 300 |
| SVDSMWIKEN | SQQTKAQTKK | NSWHQGDFSY | LRQERLTISS | ARVNDSGVFM | CYANNTFGSA |
| 310 | 320 | 330 | 340 | 350 | 360 |
| NVTTTLEVVD | KGFINIFPMM | NTTVFVNDGE | NVDLVVEYEA | YPKPVHRQWI | YMNRTSTDKW |
| 370 | 380 | 390 | 400 | 410 | 420 |
| DDYPKSENES | NIRYVNELHL | TRLKGTEGGT | YTFHVSNSDV | NSSVTFNVYV | NTKPEILTHD |
| 430 | 440 | 450 | 460 | 470 | 480 |
| RLVNGMLQCV | AAGFPEPTID | WYFCPGTEQR | CSVPVGPVDV | QIQNSSVSPF | GKLVVYSTID |
| 490 | 500 | 510 | 520 | 530 | 540 |
| DSTFKHNGTV | ECRAYNDVGK | SSASFNFAFK | GNSKEQIHAH | TLFTPLLIGF | VIAAGLMCIF |
| 550 | 560 | 570 | 580 | 590 | 600 |
| VMILTYKYLQ | KPMYEVQWKV | VEEINGNNYV | YIDPTQLPYD | HKWEFPRNRL | SFGKTLGAGA |
| 610 | 620 | 630 | 640 | 650 | 660 |
| FGKVVEATAY | GLIKSDAAMT | VAVKMLKPSA | HLTEREALMS | ELKVLSYLGN | HMNIVNLLGA |
| 670 | 680 | 690 | 700 | 710 | 720 |
| CTIGGPTLVI | TEYCCYGDLL | NFLRRKRDSF | ICSKQEDHAE | VALYKNLLHS | KESSCNDSTN |
| 730 | 740 | 750 | 760 | 770 | 780 |
| EYMDMKPGVS | YVVPTKADKR | RSARIGSYIE | RDVTPAIMED | DELALDLEDL | LSFSYQVAKG |
| 790 | 800 | 810 | 820 | 830 | 840 |
| MAFLASKNCI | HRDLAARNIL | LTHGRITKIC | DFGLARDIKN | DSNYVVKGNA | RLPVKWMAPE |
| 850 | 860 | 870 | 880 | 890 | 900 |
| SIFNCVYTFE | SDVWSYGIFL | WELFSLGSSP | YPGMPVDSKF | YKMIKEGFRM | LSPEHAPAEM |
| 910 | 920 | 930 | 940 | 950 | 960 |
| YDIMKTCWDA | DPLKRPTFKQ | IVQLIEKQIS | ESTNHIYSNL | ANCSPHRENP | AVDHSVRINS |
| 970 | |||||
| VGSSASSTQP | LLVHEDV |