Q94392

SS
Gene name
nsf-1
Protein name
Vesicle-fusing ATPase
Names
N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_H15N14.2
EC number
3.6.4.6: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

NSF is a member of the type II AAA+ (ATPase associated with various cellular activities) family. It plays a critical role in intracellular membrane trafficking by disassembling soluble NSF attachment protein receptor (SNARE) complexes. NSF is a ring-shaped homohexameric protein, with each protomer consisting of three domains: an N-terminal domain (N domain) and two highly conserved AAA+ domains (D1 and D2 domains). Mutations within the N-D1 linker region cause significant defects in the disassembly of the SNARE complex and the SNARE/α-SNAP complex binding activity. The middle and C-terminal region mutants of the NSF N-D1 linker have also increased basal ATPase activity.

Autoinhibitory domains (AIDs)

281-298 (The middle and C-terminal region of the NSF N-D1 linker) SS

Target domain

329-476 (D1 domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q94392

Entry ID Method Resolution Chain Position Source
AF-Q94392-F1 Predicted AlphaFoldDB

No variants for Q94392

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q94392

No associated diseases with Q94392

6 regional properties for Q94392

Type Name Position InterPro Accession
domain CDC48, N-terminal subdomain 67 - 150 IPR003338
domain ATPase, AAA-type, core 333 - 473 IPR003959-1
domain ATPase, AAA-type, core 616 - 741 IPR003959-2
conserved_site ATPase, AAA-type, conserved site 444 - 462 IPR003960
domain CDC48, domain 2 174 - 248 IPR004201
domain AAA ATPase, AAA+ lid domain 500 - 535 IPR041569

Functions

Description
EC Number 3.6.4.6 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
Golgi stack The set of thin, flattened membrane-bounded compartments, called cisternae, that form the central portion of the Golgi complex. The stack usually comprises cis, medial, and trans cisternae; the cis- and trans-Golgi networks are not considered part of the stack.

3 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
metal ion binding Binding to a metal ion.

4 GO annotations of biological process

Name Definition
Golgi to plasma membrane protein transport The directed movement of proteins from the Golgi to the plasma membrane in transport vesicles that move from the trans-Golgi network to the plasma membrane.
Golgi vesicle docking The initial attachment of a Golgi transport vesicle membrane to a target membrane, mediated by proteins protruding from the membrane of the Golgi vesicle and the target membrane.
intra-Golgi vesicle-mediated transport The directed movement of substances within the Golgi, mediated by small transport vesicles. These either fuse with the cis-Golgi or with each other to form the membrane stacks known as the cis-Golgi reticulum (network).
SNARE complex disassembly The disaggregation of the SNARE protein complex into its constituent components. The SNARE complex is a protein complex involved in membrane fusion; a stable ternary complex consisting of a four-helix bundle, usually formed from one R-SNARE and three Q-SNAREs with an ionic layer sandwiched between hydrophobic layers.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P46461 comt Vesicle-fusing ATPase 1 Drosophila melanogaster (Fruit fly) SS
P54351 Nsf2 Vesicle-fusing ATPase 2 Drosophila melanogaster (Fruit fly) SS
P46459 NSF Vesicle-fusing ATPase Homo sapiens (Human) SS
P46460 Nsf Vesicle-fusing ATPase Mus musculus (Mouse) SS
Q9QUL6 Nsf Vesicle-fusing ATPase Rattus norvegicus (Rat) SS
Q9M0Y8 NSF Vesicle-fusing ATPase Arabidopsis thaliana (Mouse-ear cress) SS
10 20 30 40 50 60
MSPVPCLSTC CCFRKIVEYS TMSWFRKSAN DSLLETNHDR IPVAPPREVR APSPRLPPSY
70 80 90 100 110 120
QTSNEKMFRV RKAPSEEHTL ANYAYVNRSD FDDKQIKHVR VNPGPAHHYI FSIRNDGSIK
130 140 150 160 170 180
PGEIAFGVPH RKWAALSLDQ EVRVTPFTFQ QSEYVGSMIL TADFNAKKNV TSEPLNADLM
190 200 210 220 230 240
AREFSIQFGG QAFSKGMQMA FRFEDKEKNK THTLSLVVKS IEGFDIGKAA AAASGASNTD
250 260 270 280 290 300
SSATKPKQIE AGELLPNSVI VFDKEEGSML NLIGKSKGKS AYRSIINPDW DFQQMGIGGL
310 320 330 340 350 360
DTEFSHIFRR AFASRVFPPE FIEQLGMKHV RGILLFGPPG TGKTLMARQI GKMLNAREPK
370 380 390 400 410 420
IVNGPQILDK YVGESESNVR KLFADAEEEW RRCGANSGLH IIIFDEIDAI CKQRGSMAGS
430 440 450 460 470 480
SSVHDTVVNQ LLSKMDGVEQ LNNILVIGMT NRRDMIDEAL LRPGRLEVQM EVSLPDETGR
490 500 510 520 530 540
LQILKIHTAR MREYNKMDPN VDLEDISKRT KNFSGAELEG LVRAAQSSAM NRLVKAGGKA
550 560 570 580 590 600
QADPDAIEKL AINSGDFDYA LENDIKPAFG RSDESLNRFL SRGMIVWGPE VTKILDEGSL
610 620 630 640 650 660
LAATVKNPEN SGFRTVVLAG AAKTGKTSLA AQMAKSSDFP FVKVISPEDT VGFSESAKCM
670 680 690 700 710 720
ALKKAFEDAK RSKLSVLLID NLERLIDYHP VGPRYSNLVI QALLVLLNAP PPAGHRLFVI
730 740 750 760 770 780
ATSSDRMFLR DMGLMDVFGD VIDIPKLTTA GQMMNVIQES NIYSDDQLPM IEQKLASICR
790 800 810 820
GEGFHGVGIK HLLELIESAR QCEADYRVPT LLNMMEGLAL NLYR