Q92383

PR
Gene name
mag1 (SPAPB24D3.04c)
Protein name
DNA-3-methyladenine glycosylase 1
Names
3-methyladenine DNA glycosidase 1, 3MEA DNA glycosylase 1
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
KEGG Pathway
spo:SPAPB24D3.04c
EC number
3.2.2.21: Hydrolyzing N-glycosyl compounds
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

8-92 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for Q92383

Entry ID Method Resolution Chain Position Source
3S6I X-ray 228 A A/D 1-228 PDB
AF-Q92383-F1 Predicted AlphaFoldDB

4 variants for Q92383

Variant ID(s) Position Change Description Diseaes Association Provenance
I_2954772_G_T 18 A>E No Jeffares_SNPs
I_2954535_A_G 97 I>T No Jeffares_SNPs
I_2954526_G_A 100 A>V No Jeffares_SNPs
I_2954338_C_T 163 D>N No Jeffares_SNPs

No associated diseases with Q92383

2 regional properties for Q92383

Type Name Position InterPro Accession
conserved_site Alkylbase DNA glycosidase, conserved site 146 - 170 IPR000035
domain HhH-GPD domain 51 - 222 IPR003265

Functions

Description
EC Number 3.2.2.21 Hydrolyzing N-glycosyl compounds
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
protein-DNA complex A macromolecular complex containing both protein and DNA molecules.

8 GO annotations of molecular function

Name Definition
alkylated DNA binding Binding to an alkylated residue in DNA.
alkylbase DNA N-glycosylase activity Catalysis of the reaction: DNA with alkylated base + H2O = DNA with abasic site + alkylated base. This reaction is the hydrolysis of DNA by cleavage of the N-C1' glycosidic bond between the target damaged DNA base and the deoxyribose sugar to remove an alkylated base, leaving an apyrimidinic or apurinic site.
damaged DNA binding Binding to damaged DNA.
DNA-1,N6-ethenoadenine N-glycosylase activity Catalysis of the reaction: DNA with 1-N6-ethenoadenine + H2O = DNA with abasic site + 1-N6-ethenoadenine. This reaction is the removal of 1,N6-ethenoadenine by cleaving the N-C1' glycosidic bond between the target damaged DNA base and the deoxyribose sugar.
DNA-3-methyladenine glycosylase activity Catalysis of the reaction: DNA containing 3-methyladenine + H2O = DNA with abasic site + 3-methyladenine. This reaction is the hydrolysis of DNA by cleavage of the N-C1' glycosidic bond between the damaged DNA 3-methyladenine and the deoxyribose sugar to remove the 3-methyladenine, leaving an abasic site.
DNA-3-methylguanine glycosylase activity Catalysis of the reaction: DNA containing 3-methylguanine + H2O = DNA with abasic site + 3-methylguanine. This reaction is the hydrolysis of DNA by cleavage of the N-C1' glycosidic bond between the damaged DNA 3-methylguanine and the deoxyribose sugar to remove the 3-methylguanine, leaving an abasic site.
DNA-7-methyladenine glycosylase activity Catalysis of the reaction: DNA containing 7-methyladenine + H2O = DNA with abasic site + 7-methyladenine. This reaction is the hydrolysis of DNA by cleavage of the N-C1' glycosidic bond between the damaged DNA 7-methyladenine and the deoxyribose sugar to remove the 7-methyladenine, leaving an abasic site.
DNA-7-methylguanine glycosylase activity Catalysis of the reaction: DNA containing 7-methylguanine + H2O = DNA with abasic site + 7-methylguanine. This reaction is the hydrolysis of DNA by cleavage of the N-C1' glycosidic bond between the damaged DNA 7-methylguanine and the deoxyribose sugar to remove the 7-methylguanine, leaving an abasic site.

2 GO annotations of biological process

Name Definition
base-excision repair, AP site formation The formation of an AP site, a deoxyribose sugar with a missing base, by DNA glycosylase which recognizes an altered base in DNA and catalyzes its hydrolytic removal. This sugar phosphate is the substrate recognized by the AP endonuclease, which cuts the DNA phosphodiester backbone at the 5' side of the altered site to leave a gap which is subsequently repaired.
DNA dealkylation involved in DNA repair The repair of alkylation damage, e.g. the removal of the alkyl group at the O6-position of guanine by O6-alkylguanine-DNA alkyltransferase (AGT).

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MTLDIEEKEE IVTSLTKAEI HLSGLDENWK RLVKLVGNYR PNRSMEKKEP YEELIRAVAS
70 80 90 100 110 120
QQLHSKAANA IFNRFKSISN NGQFPTPEEI RDMDFEIMRA CGFSARKIDS LKSIAEATIS
130 140 150 160 170 180
GLIPTKEEAE RLSNEELIER LTQIKGIGRW TVEMLLIFSL NRDDVMPADD LSIRNGYRYL
190 200 210 220
HRLPKIPTKM YVLKHSEICA PFRTAAAWYL WKTSKLADYT KPVRPKKH