Q8X0X0

PR
Gene name
gcs-1 (123A4.220, NCU01157)
Protein name
Glutamate--cysteine ligase
Names
Gamma-ECS, GCS, Gamma-glutamylcysteine synthetase
Species
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
KEGG Pathway
ncr:NCU01157
EC number
6.3.2.2: Acid--amino-acid ligases (peptide synthases)
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

497-572 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q8X0X0

Entry ID Method Resolution Chain Position Source
AF-Q8X0X0-F1 Predicted AlphaFoldDB

No variants for Q8X0X0

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q8X0X0

No associated diseases with Q8X0X0

4 regional properties for Q8X0X0

Type Name Position InterPro Accession
domain Aspartyl/Glutamyl-tRNA(Gln) amidotransferase, subunit B/E, catalytic 9 - 437 IPR006075
conserved_site Glutamyl-tRNA(Gln) amidotransferase, subunit B, conserved site 175 - 189 IPR017958
domain Asn/Gln amidotransferase 475 - 617 IPR018027
domain GAD domain 291 - 381 IPR029351

Functions

Description
EC Number 6.3.2.2 Acid--amino-acid ligases (peptide synthases)
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
glutamate-cysteine ligase complex An enzyme complex that catalyzes the ligation of glutamate to cysteine, forming glutamylcysteine.

2 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
glutamate-cysteine ligase activity Catalysis of the reaction: L-cysteine + L-glutamate + ATP = L-gamma-glutamyl-L-cysteine + ADP + 2 H(+) + phosphate.

1 GO annotations of biological process

Name Definition
glutathione biosynthetic process The chemical reactions and pathways resulting in the formation of glutathione, the tripeptide glutamylcysteinylglycine, which acts as a coenzyme for some enzymes and as an antioxidant in the protection of sulfhydryl groups in enzymes and other proteins.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MGLLALGTAL DWPDAKQQAH LVRAWGIKQL LEIWNKAKGK ERDAMLWGDE IEYLVVNYSK
70 80 90 100 110 120
DEPKVLLSLR QADILKALAD SKTLKVTGDC APGTEANADA GNVTLPVFHP EFGRFMLEAT
130 140 150 160 170 180
PGKPWGIDFK DLLTVEADMK LRRCIAKDHM LSNEHPITLT TFPRIGSPGV FTEPSYPVSG
190 200 210 220 230 240
PKLRSQFVPD EIANPHIRFP TLAANIRSRR GRKVQVNVPV FRDENTPWPW KDPTVNYDLH
250 260 270 280 290 300
NWPEDDDVRN GAAPDNFIHM DAMAFGMGSC CLQITFQAKN ITEGRKMYDQ LSPLGPILLA
310 320 330 340 350 360
LTAATPVYKG FIADTDVRWN QISRAVDDRT PEELGEKPLK NDRWRIPKSR YASNSTYIST
370 380 390 400 410 420
DSRLRPEYMD PNLVIDPEIK QQLLDGGMDD RLATHFAHLF IRDPIVIFNE DLQELDLTKT
430 440 450 460 470 480
DHFENIQSTN WQHMRFKPPP ADNSIGWRVE FRPMEIQITD FENAAFSVFI VLITRAILSF
490 500 510 520 530 540
DLNFYIPIVK VDENMETAHA RNANLEKKFW FRKNPFPVRT TRRGGSASRS ASGTSTPNSG
550 560 570 580 590 600
SSRPATPPLG PVEDEYRLMS VNEVINGTAY AKATSKEVTE DAEEGEEFPG LIPLVESYLD
610 620 630 640 650 660
SVNMDVATRC GLARYLDLIR KRASGELWTA AKWIREFIAK HPGYKKDSVV SEEITKDLVG
670 680 690 700 710 720
AVIEIGEREK RGLGIDDLIG QVPDLEKLFG GFLKGKSPCG GGQAALEQKL KDDDAKVNGV
KRKFNEEQ