Q8NQE1

PR
Gene name
pafA
Protein name
Pup--protein ligase
Names
Proteasome accessory factor A, Pup-conjugating enzyme
Species
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
KEGG Pathway
cgl:Cgl1494
EC number
6.3.1.19: Acid--ammonia (or amine) ligases (amide synthases)
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

165-241 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

3 structures for Q8NQE1

Entry ID Method Resolution Chain Position Source
4B0T X-ray 216 A A/B 1-482 PDB
4BJR X-ray 280 A A/B 2-482 PDB
AF-Q8NQE1-F1 Predicted AlphaFoldDB

No variants for Q8NQE1

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q8NQE1

No associated diseases with Q8NQE1

No regional properties for Q8NQE1

Type Name Position InterPro Accession
No domain, repeats, and functional sites for Q8NQE1

Functions

Description
EC Number 6.3.1.19 Acid--ammonia (or amine) ligases (amide synthases)
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

No GO annotations of cellular component

Name Definition
No GO annotations for cellular component

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ligase activity, forming carbon-nitrogen bonds Catalysis of the joining of two molecules, or two groups within a single molecule, via a carbon-nitrogen bond, with the concomitant hydrolysis of the diphosphate bond in ATP or a similar triphosphate.
magnesium ion binding Binding to a magnesium (Mg) ion.
ubiquitin-like protein transferase activity Catalysis of the transfer of a ubiquitin-like from one protein to another via the reaction X-ULP + Y --> Y-ULP + X, where both X-ULP and Y-ULP are covalent linkages. ULP represents a ubiquitin-like protein.

3 GO annotations of biological process

Name Definition
modification-dependent protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent modification of the target protein.
proteasomal protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds that is mediated by the proteasome.
protein pupylation The process in which a Pup protein is conjugated to a target protein via an isopeptide bond between the carboxy-terminus of Pup and the epsilon-amino group of a lysine residue of the target protein.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MSTVESALTR RIMGIETEYG LTFVDGDSKK LRPDEIARRM FRPIVEKYSS SNIFIPNGSR
70 80 90 100 110 120
LYLDVGSHPE YATAECDNLT QLINFEKAGD VIADRMAVDA EESLAKEDIA GQVYLFKNNV
130 140 150 160 170 180
DSVGNSYGCH ENYLVGRSMP LKALGKRLMP FLITRQLICG AGRIHHPNPL DKGESFPLGY
190 200 210 220 230 240
CISQRSDHVW EGVSSATTRS RPIINTRDEP HADSHSYRRL HVIVGDANMA EPSIALKVGS
250 260 270 280 290 300
TLLVLEMIEA DFGLPSLELA NDIASIREIS RDATGSTLLS LKDGTTMTAL QIQQVVFEHA
310 320 330 340 350 360
SKWLEQRPEP EFSGTSNTEM ARVLDLWGRM LKAIESGDFS EVDTEIDWVI KKKLIDRFIQ
370 380 390 400 410 420
RGNLGLDDPK LAQVDLTYHD IRPGRGLFSV LQSRGMIKRW TTDEAILAAV DTAPDTTRAH
430 440 450 460 470 480
LRGRILKAAD TLGVPVTVDW MRHKVNRPEP QSVELGDPFS AVNSEVDQLI EYMTVHAESY
RS