Q8L6J3

PR
Gene name
RPOT2-TOM
Protein name
DNA-directed RNA polymerase 2B, chloroplastic/mitochondrial
Names
NictaRpoT2-tom, T7 bacteriophage-type single subunit RNA polymerase 2B
Species
Nicotiana tabacum (Common tobacco)
KEGG Pathway
nta:107765810
EC number
2.7.7.6: Nucleotidyltransferases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

112-223 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q8L6J3

Entry ID Method Resolution Chain Position Source
AF-Q8L6J3-F1 Predicted AlphaFoldDB

No variants for Q8L6J3

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q8L6J3

No associated diseases with Q8L6J3

2 regional properties for Q8L6J3

Type Name Position InterPro Accession
domain DNA-directed RNA polymerase, N-terminal 188 - 507 IPR029262
domain DNA-directed RNA polymerase, C-terminal domain, phage-type 630 - 1021 IPR046950

Functions

Description
EC Number 2.7.7.6 Nucleotidyltransferases
Subcellular Localization
  • Plastid, chloroplast
  • Mitochondrion
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
chloroplast A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.
mitochondrial DNA-directed RNA polymerase complex A DNA-directed RNA polymerase complex located in the mitochondrion. Mitochondrial RNA polymerase is composed of two subunits, a catalytic core, which resembles the enzymes from bacteriophage T7 and T3, and a specificity factor required for promoter recognition, which is similar to members of the eubacterial sigma factor family. In S. cerevisiae, these are encoded by the nuclear genes RPO41 and MTF1 and the specificity factor, required for promoter recognition and initiation, is not present in the elongating form.

2 GO annotations of molecular function

Name Definition
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA-directed 5'-3' RNA polymerase activity Catalysis of the reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). Utilizes a DNA template, i.e. the catalysis of DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. Can initiate a chain 'de novo'.

1 GO annotations of biological process

Name Definition
mitochondrial transcription The synthesis of RNA from a mitochondrial DNA template, usually by a specific mitochondrial RNA polymerase.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MSSTKTPISL TIKLNQFTDK PTGLDINRYH NSPIMWRNII KQLSSRTPQK LLFSSKNRTY
70 80 90 100 110 120
SFLGFGQDSV FKDNTKFRSL IPISCSNIVM GFQNLGEYLP GDEFLSRPLL KNQVNSNDFC
130 140 150 160 170 180
CRKSYASVAE AVAVSSTDAE EDVSVVDEVQ ELLTELKKEE KKQFAFRRRK QRMLTSGMGH
190 200 210 220 230 240
RKYQTLKRRQ VKVETEAWEQ AAKEYKELLF DMCEQKLAPN LPYVKSLFLG WFEPLRDKIA
250 260 270 280 290 300
EEQELCSQGK SKAAYAKYLY QLPADMMAVI TMHKLMGLLM TGGDHGTARV VQAALVIGDA
310 320 330 340 350 360
IEQEVRIHNF LEKTKKQKAE KDKQKEDGEH VTQEQEKLRK KVTNLMKKQK LRAVGQIVRR
370 380 390 400 410 420
QDDSKPWGQD AKAKVGSRLI ELLLQTAYIQ PPANQLAVDP PDIRPAFLHS VRTVAKETKS
430 440 450 460 470 480
ASRRYGIIQC DELVFKGLER TARHMVIPYM PMLVPPVKWT GYDKGGHLYL PSYVMRTHGA
490 500 510 520 530 540
RQQREAVKRA SRNQLQPVFE ALDTLGSTKW RINKRVLSVI DRIWAGGGRL ADLVDRDDAP
550 560 570 580 590 600
LPEEPDTEDE ALRTKWRWKV KSVKKENRER HSQRCDIELK LAVARKMKDE EGFFYPHNVD
610 620 630 640 650 660
FRGRAYPMHP HLNHLGSDIC RGVLVFAEGR PLGESGLRWL KIHLANLFAG GVEKLSLEGR
670 680 690 700 710 720
IAFTENHMDD IFDSADKPLE GRRWWLNAED PFQCLAVCIN LSEAVRSSSP ETSISHIPVH
730 740 750 760 770 780
QDGSCNGLQH YAALGRDELG AAAVNLVAGE KPADVYSGIA ARVLDIMKRD AQRDPAEFPD
790 800 810 820 830 840
AVRARALVNQ VDRKLVKQTV MTSVYGVTYI GARDQIKRRL KERGAIADDS ELFGAACYAA
850 860 870 880 890 900
KVTLTALGEM FEAARSIMTW LGECAKIIAS ENEPVRWTTP LGLPVVQPYR KIGRHLIKTS
910 920 930 940 950 960
LQILTLQQET EKVMVKRQRT AFPPNFIHSL DGSHMMMTAV ACRRAGLNFA GVHDSYWTHA
970 980 990 1000 1010 1020
CDVDKLNRIL REKFVELYET PILEKLLESF QTSYPTLLFP PLPERGDFDL RDVLESPYFF
N