Q8EJW3

PR
Gene name
acnD (SO_0343)
Protein name
2-methylcitrate dehydratase (2-methyl-trans-aconitate forming)
Names
Aconitate hydratase, ACN, Aconitase
Species
Shewanella oneidensis (strain MR-1)
KEGG Pathway
son:SO_0343
EC number
4.2.1.3: Hydro-lyases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-39 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q8EJW3

Entry ID Method Resolution Chain Position Source
AF-Q8EJW3-F1 Predicted AlphaFoldDB

No variants for Q8EJW3

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q8EJW3

No associated diseases with Q8EJW3

2 regional properties for Q8EJW3

Type Name Position InterPro Accession
domain Aconitase A/isopropylmalate dehydratase small subunit, swivel domain 661 - 792 IPR000573
domain Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain 74 - 537 IPR001030

Functions

Description
EC Number 4.2.1.3 Hydro-lyases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

6 GO annotations of molecular function

Name Definition
4 iron, 4 sulfur cluster binding Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
aconitate hydratase activity Catalysis of the reaction: citrate = isocitrate. The reaction occurs in two steps: (1) citrate = cis-aconitate + H2O, (2) cis-aconitate + H2O = isocitrate. This reaction is the interconversion of citrate and isocitrate via the labile, enzyme-bound intermediate cis-aconitate. Water is removed from one part of the citrate molecule and added back to a different atom to form isocitrate.
citrate dehydratase activity Catalysis of the reaction: citrate = cis-aconitate + H2O.
hydro-lyase activity Catalysis of the cleavage of a carbon-oxygen bond by elimination of water.
iron-responsive element binding Binding to an iron-responsive element, a regulatory sequence found in the 5'- and 3'-untranslated regions of mRNAs encoding many iron-binding proteins.
metal ion binding Binding to a metal ion.

4 GO annotations of biological process

Name Definition
citrate metabolic process The chemical reactions and pathways involving citrate, 2-hydroxy-1,2,3-propanetricarboyxlate. Citrate is widely distributed in nature and is an important intermediate in the TCA cycle and the glyoxylate cycle.
propionate catabolic process, 2-methylcitrate cycle The chemical reactions and pathways resulting in the breakdown of propionate that occurs in the 2-methylcitrate cycle.
propionate metabolic process, methylcitrate cycle The chemical reactions and pathways involving propionate that occur in the methylcitrate cycle.
tricarboxylic acid cycle A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MSTVMNTQYR KPLPGTALDY FDTREAIEAI APGAYAKLPY TSRVLAENLV RRCEPEMLTA
70 80 90 100 110 120
SLKQIIESKQ ELDFPWFPAR VVCHDILGQT ALVDLAGLRD AIAAKGGDPA QVNPVVPTQL
130 140 150 160 170 180
IVDHSLAVEY GGFDKDAFAK NRAIEDRRNE DRFHFINWTQ KAFKNIDVIP QGNGIMHQIN
190 200 210 220 230 240
LERMSPVIHA RNGVAFPDTL VGTDSHTPHV DALGVIAIGV GGLEAESVML GRASYMRLPD
250 260 270 280 290 300
IIGVELTGKP QPGITATDIV LALTEFLRAQ KVVSSYLEFF GEGAEALTLG DRATISNMTP
310 320 330 340 350 360
EFGATAAMFY IDQQTLDYLT LTGREAEQVK LVETYAKTAG LWSDDLKQAV YPRTLHFDLS
370 380 390 400 410 420
SVVRTIAGPS NPHARVPTSE LAARGISGEV ENEPGLMPDG AVIIAAITSC TNTSNPRNVI
430 440 450 460 470 480
AAGLLARNAN AKGLTRKPWV KTSLAPGSKA VQLYLEEANL LPELESLGFG IVGFACTTCN
490 500 510 520 530 540
GMSGALDPVI QQEVIDRDLY ATAVLSGNRN FDGRIHPYAK QAFLASPPLV VAYAIAGTIR
550 560 570 580 590 600
FDIEKDVLGL DKDGKPVRLI NIWPSDAEID AVIAASVKPE QFRKVYEPMF DLSVDYGDKV
610 620 630 640 650 660
SPLYDWRPQS TYIRRPPYWE GALAGERTLK GMRPLAVLGD NITTDHLSPS NAIMMDSAAG
670 680 690 700 710 720
EYLHKMGLPE EDFNSYATHR GDHLTAQRAT FANPKLKNEM AIVDGKVKQG SLARIEPEGI
730 740 750 760 770 780
VTRMWEAIET YMDRKQPLII IAGADYGQGS SRDWAAKGVR LAGVEAIVAE GFERIHRTNL
790 800 810 820 830 840
VGMGVLPLEF KAGENRATYG IDGTEVFDVI GSIAPRADLT VIITRKNGER VEVPVTCRLD
850 860
TAEEVSIYEA GGVLQRFAQD FLESNLK