AiPD: Autoinhibited Protein Database

Q7L8W6

Gene name	DPH6
Protein name	Diphthine--ammonia ligase
Names	ATP-binding domain-containing protein 4, Diphthamide synthase, Diphthamide synthetase, Protein DPH6 homolog
Species	Homo sapiens (Human)
KEGG Pathway	hsa:89978
EC number	6.3.1.14: Acid--ammonia (or amine) ligases (amide synthases)
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

220-267 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

220-267 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

1 structures for Q7L8W6

Entry ID	Method	Resolution	Chain	Position	Source
AF-Q7L8W6-F1	Predicted				AlphaFoldDB

219 variants for Q7L8W6

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA391923433 rs1254953034	2	R>S		No	ClinGen gnomAD
CA7468672 rs747539785	2	R>W		No	ClinGen ExAC TOPMed gnomAD
rs780683833 CA7468671	3	V>G		No	ClinGen ExAC TOPMed gnomAD
CA391923422 rs1458861247	4	A>E		No	ClinGen TOPMed
rs1485407671 CA391923419	5	A>P		No	ClinGen TOPMed gnomAD
rs1485407671 CA391923418	5	A>S		No	ClinGen TOPMed gnomAD
CA391923415 rs1201353075	5	A>V		No	ClinGen Ensembl
CA268978564 rs1053713276	6	L>P		No	ClinGen Ensembl
CA268978565 rs946682046	6	L>V		No	ClinGen TOPMed
rs1049464077 CA268978562	7	I>M		No	ClinGen TOPMed gnomAD
rs745951697 CA7468669	8	S>G		No	ClinGen ExAC gnomAD
CA391923382 rs1248163478	9	G>D		No	ClinGen gnomAD
rs1595457482 CA391923372	11	K>E		No	ClinGen Ensembl
rs1566946434 CA391923364	12	D>N		No	ClinGen Ensembl
CA7468655 rs761088772	13	S>R		No	ClinGen ExAC gnomAD
rs1269112061 CA391923353	13	S>T		No	ClinGen gnomAD
CA7468654 rs747627790	15	Y>D		No	ClinGen ExAC TOPMed gnomAD
rs1230567377 CA391923328	16	N>K		No	ClinGen gnomAD
CA391923321 rs1418267053	17	M>I		No	ClinGen gnomAD
CA391923322 rs1161756263	17	M>R		No	ClinGen TOPMed
rs933028569 CA391923315	18	M>K		No	ClinGen gnomAD
CA268978131 rs933028569	18	M>T		No	ClinGen gnomAD
rs1387671256 CA391923317	18	M>V		No	ClinGen TOPMed
CA391923308 rs1297034395	19	Q>*		No	ClinGen gnomAD
CA391923293 rs1333060648	21	I>V		No	ClinGen gnomAD
CA7468652 rs141206143	22	A>G		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA7468651 rs774490655	23	A>P		No	ClinGen ExAC TOPMed gnomAD
CA391923277 rs1358067932	24	G>R		No	ClinGen TOPMed
rs943536618 CA391923249	27	I>M		No	ClinGen TOPMed gnomAD
rs148139655 CA391923247	28	V>F		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA7468649 rs148139655	28	V>I		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs777953049 CA7468648	30	L>S		No	ClinGen ExAC gnomAD
rs1383499100 CA391923228	31	A>G		No	ClinGen TOPMed
CA7468647 rs755947387	31	A>T		No	ClinGen ExAC gnomAD
rs373612094 CA391923197	36	A>D		No	ClinGen TOPMed gnomAD
CA268978128 rs373612094	36	A>G		No	ClinGen TOPMed gnomAD
rs1007187617 CA268978127	39	Q>L		No	ClinGen TOPMed
rs1030054031 CA268978126	40	V>M		No	ClinGen TOPMed gnomAD
CA7468636 rs34907758	41	G>A		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA7468635 VAR_031403 RCV000957254 rs34907758	41	G>E		No	ClinGen ClinVar UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA391923142 rs1444182293	43	D>A		No	ClinGen gnomAD
CA7468634 rs774673219	44	E>G		No	ClinGen ExAC gnomAD
rs981952887 CA268977735	44	E>K		No	ClinGen Ensembl
rs1200091335 CA391923131	45	L>P		No	ClinGen gnomAD
CA391923123 rs1375428907	46	D>E		No	ClinGen TOPMed
rs143353998 CA7468633	46	D>N		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA268977734 rs866968402	49	M>I		No	ClinGen Ensembl
rs749336463 CA7468632	49	M>T		No	ClinGen ExAC gnomAD
rs1229415944 CA391923095	50	Y>C		No	ClinGen gnomAD
rs112008207 CA268977733	50	Y>H		No	ClinGen Ensembl
CA391923090 rs773489733	51	Q>*		No	ClinGen ExAC gnomAD
rs773489733 CA7468631	51	Q>K		No	ClinGen ExAC gnomAD
rs1239205170 CA391923083	52	T>A		No	ClinGen TOPMed gnomAD
rs769995089 CA7468630	52	T>I		No	ClinGen ExAC gnomAD
CA391923056 rs1468903880	56	H>R		No	ClinGen gnomAD
CA391923057 rs1566945084	56	H>Y		No	ClinGen Ensembl
CA391923051 rs1403469095	57	A>T		No	ClinGen gnomAD
CA391923044 rs1161056076	58	I>V		No	ClinGen gnomAD
CA391923032 COSM960946 rs1334637909 COSM960947	59	D>E	Variant assessed as Somatic; impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated NCI-TCGA TOPMed gnomAD
CA7468627 rs768297939	60	L>*		No	ClinGen ExAC gnomAD
rs747013601 CA7468626	61	Y>C		No	ClinGen ExAC TOPMed gnomAD
rs747013601 CA391923020	61	Y>F		No	ClinGen ExAC TOPMed gnomAD
rs779821700 CA7468625	64	A>T		No	ClinGen ExAC gnomAD
rs1238060633 CA391922995	65	M>T		No	ClinGen gnomAD
rs757990199 CA7468624	65	M>V		No	ClinGen ExAC TOPMed gnomAD
rs1299658976 CA391922982	67	L>F		No	ClinGen TOPMed
TCGA novel	67	L>I	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	69	L>I	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1183083757 CA391922962	70	Y>C		No	ClinGen gnomAD
rs749935491 CA7468623	71	R>C		No	ClinGen ExAC TOPMed gnomAD
CA7468622 rs778508898	71	R>H		No	ClinGen ExAC TOPMed gnomAD
rs371901885 CA7468620	72	R>*	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ESP ExAC NCI-TCGA gnomAD
rs374490007 CA7468619 COSM1640214 COSM1640213	72	R>Q	stomach [Cosmic]	No	ClinGen cosmic curated 1000Genomes ESP ExAC TOPMed gnomAD
rs1282670869 CA391922939	74	I>M		No	ClinGen gnomAD
CA391922944 rs1310540683	74	I>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
CA391922928 rs1459814740	76	G>E		No	ClinGen gnomAD
rs1324909057 CA391922931	76	G>R		No	ClinGen TOPMed gnomAD
rs1284215433 CA391922894	81	T>A		No	ClinGen TOPMed gnomAD
TCGA novel	86	T>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs113342947 CA268977731	87	K>R		No	ClinGen Ensembl
CA7468615 rs763082163	88	C>R		No	ClinGen ExAC gnomAD
CA7468614 rs773296974	88	C>Y	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
rs868488195 CA268977730	90	G>A		No	ClinGen Ensembl
TCGA novel	91	D>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA391922817 rs1156669248	92	E>K	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA gnomAD
TCGA novel	94	E>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs762055995 CA7468612	96	L>F		No	ClinGen ExAC gnomAD
rs370450320 CA268977728	103	V>G		No	ClinGen ESP TOPMed
rs765990539 CA268977729	103	V>L		No	ClinGen Ensembl
CA391922120 rs1234610588	108	E>D		No	ClinGen TOPMed
CA268968655 rs1011898386 COSM554958	108	E>K	lung Variant assessed as Somatic; impact. [Cosmic, NCI-TCGA]	No	ClinGen cosmic curated NCI-TCGA gnomAD
rs781364288 CA7468533	108	E>V		No	ClinGen ExAC TOPMed gnomAD
rs755257307 CA7468531	110	E>K		No	ClinGen ExAC gnomAD
rs751794681 CA7468529	112	I>M		No	ClinGen ExAC gnomAD
TCGA novel	116	A>D	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1393382098 CA391922063	117	I>M		No	ClinGen gnomAD
CA391922068 rs1380917286	117	I>V		No	ClinGen gnomAD
rs766114531 CA7468528	121	Y>C		No	ClinGen ExAC gnomAD
CA268968654 rs201383115	123	R>C		No	ClinGen 1000Genomes TOPMed gnomAD
CA7468526 rs146910158	123	R>H		No	ClinGen ESP ExAC TOPMed gnomAD
CA7468525 rs750370056	124	I>V		No	ClinGen ExAC gnomAD
rs757556558 CA7468524	125	R>*	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
rs1450889968 CA391922014	125	R>L		No	ClinGen TOPMed gnomAD
CA391922016 rs1450889968	125	R>Q	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed gnomAD
rs1373320456 CA391922013	126	V>M		No	ClinGen gnomAD
rs761595092 CA7468523	128	N>S		No	ClinGen ExAC gnomAD
CA391921969 rs1407801319	130	C>S		No	ClinGen TOPMed gnomAD
CA391921970 rs1407801319	130	C>Y		No	ClinGen TOPMed gnomAD
rs767186937 CA7468495	131	K>E		No	ClinGen ExAC TOPMed gnomAD
CA391921960 rs1176839699	131	K>N		No	ClinGen TOPMed gnomAD
rs1566914433 CA391921949	133	L>F		No	ClinGen Ensembl
rs759054480 CA7468492	136	Q>*		No	ClinGen ExAC TOPMed gnomAD
CA268968209 rs201959314	136	Q>H		No	ClinGen Ensembl
rs1179774655 CA391921914	138	L>F		No	ClinGen TOPMed gnomAD
rs1444301622 CA391921910	139	A>S	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA gnomAD
rs1243939129 CA391921903	140	Y>C		No	ClinGen gnomAD
CA391921877 rs930768927	143	Q>H		No	ClinGen TOPMed gnomAD
TCGA novel	143	Q>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1234218775 CA391921845	147	E>D		No	ClinGen TOPMed
CA7468490 rs770014356	149	L>F		No	ClinGen ExAC gnomAD
rs920672304 CA268968206	152	E>D		No	ClinGen TOPMed gnomAD
rs748690837 CA7468489	153	M>I		No	ClinGen ExAC gnomAD
rs867195336 CA268968205	154	I>M		No	ClinGen TOPMed
rs1455563612 CA391921783	157	N>D		No	ClinGen TOPMed
CA7468486 rs533235178	158	I>F		No	ClinGen 1000Genomes ExAC gnomAD
rs1174161687 CA391921774	158	I>T		No	ClinGen TOPMed
rs533235178 CA7468485	158	I>V		No	ClinGen 1000Genomes ExAC gnomAD
CA7468484 rs779936508	159	Q>K		No	ClinGen ExAC TOPMed gnomAD
CA7468483 rs758649328	159	Q>R		No	ClinGen ExAC gnomAD
rs990622214 CA268968204	161	M>I		No	ClinGen TOPMed gnomAD
CA7468480 rs757023821	161	M>K		No	ClinGen ExAC gnomAD
rs750653114 CA7468482	161	M>L		No	ClinGen ExAC TOPMed gnomAD
rs757023821 CA391921758	161	M>T		No	ClinGen ExAC gnomAD
CA7468481 rs750653114	161	M>V		No	ClinGen ExAC TOPMed gnomAD
CA7468479 rs370800281	162	I>N		No	ClinGen ESP ExAC TOPMed gnomAD
CA391921728 rs1302860856	165	V>G		No	ClinGen TOPMed
rs565863034 CA7468478	168	L>F		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA391922280 rs1297244652	171	D>G		No	ClinGen gnomAD
rs1400157608 CA391922283	171	D>N		No	ClinGen TOPMed
rs1273260594 CA391922276	172	P>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA gnomAD
rs142323760 CA7468456	173	D>A		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs142323760 CA7468457	173	D>G		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA268963723 rs887731679	173	D>H		No	ClinGen TOPMed
CA391922252 rs1337174800	175	H>L		No	ClinGen gnomAD
rs1361003096 CA391922257	175	H>Y		No	ClinGen gnomAD
rs1415279658 CA391922233	178	K>R		No	ClinGen gnomAD
rs1316183542 CA391922226	179	T>I		No	ClinGen TOPMed
rs1366141217 CA391922229	179	T>P		No	ClinGen gnomAD
rs754256854 CA7468452	180	L>P		No	ClinGen ExAC TOPMed gnomAD
CA391922221 rs754256854	180	L>Q		No	ClinGen ExAC TOPMed gnomAD
CA391922222 rs1302588681	180	L>V		No	ClinGen gnomAD
rs761263253 CA7468450	181	D>V		No	ClinGen ExAC gnomAD
CA268963722 rs978396059	183	M>K		No	ClinGen Ensembl
rs775834601 CA7468449	183	M>V		No	ClinGen ExAC gnomAD
CA391922196 rs1417732927	184	E>Q		No	ClinGen TOPMed gnomAD
rs965501893 CA268963721	185	P>L		No	ClinGen TOPMed
rs759530818 CA7468447	186	Y>C		No	ClinGen ExAC gnomAD
CA268963720 rs1006902430	188	I>K		No	ClinGen Ensembl
rs199528007 CA7468446	188	I>V		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA7468445 rs771154609	189	E>K		No	ClinGen ExAC gnomAD
COSM1707988 rs1454773017 CA391921691	190	L>F	skin [Cosmic]	No	ClinGen cosmic curated TOPMed
rs1167481887 CA391921680	192	K>E		No	ClinGen TOPMed
CA7468430 rs149996784	193	K>N		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs760030551 CA7468429	194	Y>C		No	ClinGen ExAC gnomAD
CA7468427 rs770958547	195	G>E	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
CA268960488 rs866750356	199	C>R		No	ClinGen Ensembl
rs763186764 CA7468426	199	C>Y		No	ClinGen ExAC gnomAD
rs773401816 CA7468425	200	G>A		No	ClinGen ExAC TOPMed gnomAD
rs769674669 CA7468424	201	E>K		No	ClinGen ExAC gnomAD
rs953949304 CA268960487	202	G>S		No	ClinGen Ensembl
rs1167780757 CA391921601	204	E>G		No	ClinGen TOPMed gnomAD
rs974276367 CA268960486	207	T>I		No	ClinGen Ensembl
CA7468421 rs768494184	208	F>L		No	ClinGen ExAC TOPMed gnomAD
rs987622986 CA268960485	213	P>A		No	ClinGen TOPMed gnomAD
rs765558502 CA7468418	213	P>L		No	ClinGen ExAC TOPMed gnomAD
CA7468419 rs765558502	213	P>R		No	ClinGen ExAC TOPMed gnomAD
rs750137666 CA7468417	216	K>R		No	ClinGen ExAC TOPMed gnomAD
CA391921500 rs1267997569	219	I>V		No	ClinGen TOPMed
rs1338930124 CA391921487	221	V>M		No	ClinGen TOPMed gnomAD
rs139330319 CA7468393	222	D>Y		No	ClinGen ESP ExAC TOPMed gnomAD
rs1037836040 CA268959528	225	E>D		No	ClinGen Ensembl
rs750519127 CA7468390	227	V>L		No	ClinGen ExAC gnomAD
CA391921420 rs1487273441	229	H>R		No	ClinGen TOPMed gnomAD
rs1358868996 CA391921403	232	D>N		No	ClinGen gnomAD
CA7468386 rs763833562	233	A>T		No	ClinGen ExAC gnomAD
rs10519996 CA391921373	236	P>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs10519996 CA7468383 VAR_031404	236	P>R		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA7468384 rs775626252	236	P>S		No	ClinGen ExAC gnomAD
COSM1678347 CA7468382 rs745733884	239	Y>C	prostate [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
CA7468381 rs547000751	241	R>C		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs764647898 CA7468379	241	R>H		No	ClinGen ExAC TOPMed gnomAD
CA7468380 rs764647898	241	R>L		No	ClinGen ExAC TOPMed gnomAD
rs777452839 CA7468378	242	F>L		No	ClinGen ExAC gnomAD
rs1463199774 CA391921321	245	L>*		No	ClinGen gnomAD
rs1222855547 CA391921309	246	H>Q		No	ClinGen TOPMed
CA391921314 rs1373373096	246	H>Y		No	ClinGen gnomAD
CA391921301 rs755403757	248	E>K		No	ClinGen ExAC TOPMed gnomAD
rs755403757 CA7468375	248	E>Q		No	ClinGen ExAC TOPMed gnomAD
rs780431810 CA391921266	251	V>L		No	ClinGen ExAC TOPMed gnomAD
rs780431810 CA7468352	251	V>M		No	ClinGen ExAC TOPMed gnomAD
CA7468351 rs370898941	252	S>F		No	ClinGen ESP ExAC TOPMed gnomAD
rs974588124 CA268959401	253	S>L		No	ClinGen TOPMed gnomAD
rs1264097183 CA391921257	253	S>P		No	ClinGen TOPMed
CA391921247 rs1307909085	254	V>M		No	ClinGen TOPMed gnomAD
rs1384573771 CA391921237	255	P>H		No	ClinGen gnomAD
CA391921238 rs1237439915	255	P>S		No	ClinGen TOPMed gnomAD
CA7468349 rs779468977	257	N>S		No	ClinGen ExAC TOPMed gnomAD
CA268959399 rs965195203	259	R>T		No	ClinGen TOPMed
rs1320201496 CA391921196	260	T>I		No	ClinGen gnomAD
rs1320201496 CA391921197	260	T>K		No	ClinGen gnomAD
CA391921198 rs1358557705	260	T>S		No	ClinGen TOPMed
rs757335464 CA7468348	261	S>C		No	ClinGen ExAC TOPMed gnomAD
CA391921171 rs1446627656	263	Y>F		No	ClinGen gnomAD
CA7468346 rs200030258	264	I>T		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1181546190 CA391921167	264	I>V		No	ClinGen gnomAD
rs1445314088 CA391921155	265	Y>C		No	ClinGen TOPMed
CA391921158 rs1595506594	265	Y>H		No	ClinGen Ensembl
CA391921146 rs1244262370	266	N>S		No	ClinGen gnomAD
rs1285641693 CA391921127	268	F>L		No	ClinGen TOPMed

No associated diseases with Q7L8W6

1 regional properties for Q7L8W6

Type	Name	Position	InterPro Accession
domain	AP2/ERF domain	26 - 90	IPR001471

Functions

		Description
EC Number	6.3.1.14	Acid--ammonia (or amine) ligases (amide synthases)
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

3 GO annotations of cellular component

Name	Definition
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nucleolus	A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleoplasm	That part of the nuclear content other than the chromosomes or the nucleolus.

2 GO annotations of molecular function

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
diphthine-ammonia ligase activity	Catalysis of the reaction: ATP + diphthine + NH(4)(+) = ADP + diphthamide + H(+) + phosphate.

1 GO annotations of biological process

Name	Definition
peptidyl-diphthamide biosynthetic process from peptidyl-histidine	The modification of peptidyl-histidine to 2'-(3-carboxamido-3-(trimethylammonio)propyl)-L-histidine, known as diphthamide, found in translation elongation factor EF-2. The process occurs in eukaryotes and archaea but not eubacteria.

No homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
No homologous proteins

10	20	30	40	50	60
MRVAALISGG	KDSCYNMMQC	IAAGHQIVAL	ANLRPAENQV	GSDELDSYMY	QTVGHHAIDL
70	80	90	100	110	120
YAEAMALPLY	RRTIRGRSLD	TRQVYTKCEG	DEVEDLYELL	KLVKEKEEVE	GISVGAILSD
130	140	150	160	170	180
YQRIRVENVC	KRLNLQPLAY	LWQRNQEDLL	REMISSNIQA	MIIKVAALGL	DPDKHLGKTL
190	200	210	220	230	240
DQMEPYLIEL	SKKYGVHVCG	EGGEYETFTL	DCPLFKKKII	VDSSEVVIHS	ADAFAPVAYL
250	260
RFLELHLEDK	VSSVPDNYRT	SNYIYNF