AiPD: Autoinhibited Protein Database

Q5ZJW4

Gene name	SEC22B (RCJMB04_15c3)
Protein name	Vesicle-trafficking protein SEC22b
Names	SEC22 vesicle-trafficking protein homolog B
Species	Homo sapiens (Human)
KEGG Pathway	gga:424377
EC number
Protein Class

Descriptions

Phagocyte NADPH oxidase is a multisubunit enzyme responsible for the production of reactive oxygen species. NCF1 (p47phox) is a cytosolic component of the NADPH oxidase and plays an important role in the assembly of the activated complex. NCF1 is composed of several domains including a PX domain, tandem SH3 domains (N-SH3 and C-SH3), a polybasic/autoinhibitory region (PBR/AIR), and a proline-rich region (PRR). In an inactive state, the binding surface of the tandem SH3 domains for the binding to the PRR region of p22phox (CYBA) is masked through the intramolecular interaction with PBR/AIR. Phosphorylations of the Ser residues in PBR/AIR by PKC or Akt cause a conformational change in NCF1 and activation of the NADPH oxidase.

Autoinhibitory domains (AIDs)

1-119 (Longin domain) SS

Target domain	134-194 (v-SNARE coiled-coil homology)
Relief mechanism	Others, PTM
Assay

AID

1-119 (Longin domain)

Target domain

134-194 (v-SNARE coiled-coil homology)

Relief mechanism

Others (self-oligomerization through SNARE domain), PTM (Removal of farnesyl group from the C-terminal tail, Palmitoylation of the C-terminal tail

Assay

Accessory elements

No accessory elements

References

Gonzalez LC Jr et al. (2001) "A novel snare N-terminal domain revealed by the crystal structure of Sec22b", The Journal of biological chemistry, 276, 24203-11

Wen W et al. (2010) "Lipid-Induced conformational switch controls fusion activity of longin domain SNARE Ykt6", Molecular cell, 37, 383-95

Mancias JD et al. (2007) "The transport signal on Sec22 for packaging into COPII-coated vesicles is a conformational epitope", Molecular cell, 26, 403-14

Autoinhibited structure

Activated structure

1 structures for Q5ZJW4

Entry ID	Method	Resolution	Chain	Position	Source
AF-Q5ZJW4-F1	Predicted				AlphaFoldDB

7 variants for Q5ZJW4

Variant ID(s)	Position	Change	Diseaes Association	Provenance
rs740117324	5	T>P	No	EVA
rs736484234	58	A>G	No	EVA
rs732939134	112	F>L	No	EVA
rs736500333	113	I>N	No	EVA
rs741253532	114	E>*	No	EVA
rs736580516	145	V>G	No	EVA
rs738887418	216	L>W	No	EVA

No associated diseases with Q5ZJW4

4 regional properties for Q5ZJW4

Type	Name	Position	InterPro Accession
domain	Zinc finger, UBR-type	97 - 168	IPR003126
domain	Adaptor protein ClpS, core	223 - 300	IPR003769
domain	E3 ubiquitin-protein ligase UBR-like, C-terminal	1312 - 1730	IPR044046
domain	E3 ubiquitin-protein ligase UBR2, UBR-box	98 - 167	IPR047508

Functions

		Description
EC Number
Subcellular Localization	Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

7 GO annotations of cellular component

Name	Definition
endoplasmic reticulum membrane	The lipid bilayer surrounding the endoplasmic reticulum.
endoplasmic reticulum-Golgi intermediate compartment	A complex system of membrane-bounded compartments located between endoplasmic reticulum (ER) and the Golgi complex, with a distinctive membrane protein composition; involved in ER-to-Golgi and Golgi-to-ER transport.
endoplasmic reticulum-Golgi intermediate compartment membrane	The lipid bilayer surrounding any of the compartments of the endoplasmic reticulum (ER)-Golgi intermediate compartment system.
ER to Golgi transport vesicle membrane	The lipid bilayer surrounding a vesicle transporting substances from the endoplasmic reticulum to the Golgi.
Golgi membrane	The lipid bilayer surrounding any of the compartments of the Golgi apparatus.
melanosome	A tissue-specific, membrane-bounded cytoplasmic organelle within which melanin pigments are synthesized and stored. Melanosomes are synthesized in melanocyte cells.
SNARE complex	A protein complex involved in membrane fusion; a stable ternary complex consisting of a four-helix bundle, usually formed from one R-SNARE and three Q-SNAREs with an ionic layer sandwiched between hydrophobic layers. One well-characterized example is the neuronal SNARE complex formed of synaptobrevin 2, syntaxin 1a, and SNAP-25.

1 GO annotations of molecular function

Name	Definition
SNAP receptor activity	Acting as a marker to identify a membrane and interacting selectively with one or more SNAREs on another membrane to mediate membrane fusion.

6 GO annotations of biological process

Name	Definition
endoplasmic reticulum to Golgi vesicle-mediated transport	The directed movement of substances from the endoplasmic reticulum (ER) to the Golgi, mediated by COP II vesicles. Small COP II coated vesicles form from the ER and then fuse directly with the cis-Golgi. Larger structures are transported along microtubules to the cis-Golgi.
negative regulation of autophagosome assembly	Any process that stops, prevents or reduces the frequency, rate or extent of autophagosome assembly.
positive regulation of protein catabolic process	Any process that activates or increases the frequency, rate or extent of the chemical reactions and pathways resulting in the breakdown of a protein by the destruction of the native, active configuration, with or without the hydrolysis of peptide bonds.
protein transport	The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum	The directed movement of substances from the Golgi back to the endoplasmic reticulum, mediated by vesicles bearing specific protein coats such as COPI or COG.
vesicle fusion with Golgi apparatus	The joining of the lipid bilayer membrane around a vesicle to the lipid bilayer membrane around the Golgi.

7 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
O75396	SEC22B	Vesicle-trafficking protein SEC22b	Homo sapiens (Human)	SS
O08547	Sec22b	Vesicle-trafficking protein SEC22b	Mus musculus (Mouse)	EV
Q4KM74	Sec22b	Vesicle-trafficking protein SEC22b	Rattus norvegicus (Rat)	SS
Q94AU2	SEC22	25.3 kDa vesicle transport protein	Arabidopsis thaliana (Mouse-ear cress)	PR
Q6P7L4	sec22b	Vesicle-trafficking protein SEC22b	Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)	SS
Q7SXP0	sec22bb	Vesicle-trafficking protein SEC22b-B	Danio rerio (Zebrafish) (Brachydanio rerio)	SS
Q7ZV15	sec22ba	Vesicle-trafficking protein SEC22b-A	Danio rerio (Zebrafish) (Brachydanio rerio)	SS

10	20	30	40	50	60
MYMFLVKWQD	LSEKVVYRRF	TEIYEFHKTL	KEMFPIEAGA	INPENRIIPH	LPAPKWFDGQ
70	80	90	100	110	120
RAAENHQGTL	TEYCSTLMSL	PTKISRCPHL	LDFFKVRPDD	LKLPTDNQTK	KPETYLMPKD
130	140	150	160	170	180
GKSTATDITG	PIILQTYRAI	ADYEKTSGSE	MALSTGDVVE	VVEKSESGWW	FCQMKAKRGW
190	200	210	220	230	240
IPASFLEPLD	SPDETEDPEP	NYAGEPYVAI	KAYTAVEGDE	VSLLEGEAVE	VIHKLLDGWW
250	260	270	280	290	300
VIRKDDVTGY	FPSMYLQKSG	QDVSQAQRQI	KRGAPPRRSS	IRNAHSIHQR	SRKRLSQDAY
310	320	330	340	350	360
RRNSVRFLQQ	RRRQARPGPQ	SPGSPLEEER	QTQRSKPQPA	VPPRPSADLI	LNRCSESTKR

KLASAV