Q5R4I2

PR
Gene name
RNF8
Protein name
E3 ubiquitin-protein ligase RNF8
Names
RING finger protein 8, RING-type E3 ubiquitin transferase RNF8
Species
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
KEGG Pathway
pon:100173982
EC number
2.3.2.27: Aminoacyltransferases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

235-323 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q5R4I2

Entry ID Method Resolution Chain Position Source
AF-Q5R4I2-F1 Predicted AlphaFoldDB

No variants for Q5R4I2

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q5R4I2

No associated diseases with Q5R4I2

3 regional properties for Q5R4I2

Type Name Position InterPro Accession
domain Forkhead-associated (FHA) domain 37 - 109 IPR000253
domain Zinc finger, RING-type 404 - 442 IPR001841
conserved_site Zinc finger, RING-type, conserved site 419 - 428 IPR017907

Functions

Description
EC Number 2.3.2.27 Aminoacyltransferases
Subcellular Localization
  • Nucleus
  • Cytoplasm
  • Midbody
  • Chromosome, telomere
  • Recruited at uncapped telomeres
  • Following DNA double-strand breaks, recruited to the sites of damage
  • During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern
  • In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm
  • In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells
  • Does not seem to be associated with condensed chromosomes at any time during the cell cycle
  • During spermatogenesis, sequestered in the cytoplasm by PIWIL1: RNF8 is released following ubiquitination and degradation of PIWIL1
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
chromosome, telomeric region The end of a linear chromosome, required for the integrity and maintenance of the end. A chromosome telomere usually includes a region of telomerase-encoded repeats the length of which rarely exceeds 20 bp each and that permits the formation of a telomeric loop (T-loop). The telomeric repeat region is usually preceded by a sub-telomeric region that is gene-poor but rich in repetitive elements. Some telomeres only consist of the latter part (for eg. D. melanogaster telomeres).
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
midbody A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
site of double-strand break A region of a chromosome at which a DNA double-strand break has occurred. DNA damage signaling and repair proteins accumulate at the lesion to respond to the damage and repair the DNA to form a continuous DNA helix.
ubiquitin ligase complex A protein complex that includes a ubiquitin-protein ligase and enables ubiquitin protein ligase activity. The complex also contains other proteins that may confer substrate specificity on the complex.

6 GO annotations of molecular function

Name Definition
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
histone binding Binding to a histone, any of a group of water-soluble proteins found in association with the DNA of eukaryotic or archaeal chromosomes. They are involved in the condensation and coiling of chromosomes during cell division and have also been implicated in gene regulation and DNA replication. They may be chemically modified (methylated, acetlyated and others) to regulate gene transcription.
protein homodimerization activity Binding to an identical protein to form a homodimer.
ubiquitin binding Binding to ubiquitin, a protein that when covalently bound to other cellular proteins marks them for proteolytic degradation.
ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.
zinc ion binding Binding to a zinc ion (Zn).

17 GO annotations of biological process

Name Definition
cell cycle The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division.
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
cellular response to DNA damage stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
double-strand break repair The repair of double-strand breaks in DNA via homologous and nonhomologous mechanisms to reform a continuous DNA helix.
double-strand break repair via nonhomologous end joining The repair of a double-strand break in DNA in which the two broken ends are rejoined with little or no sequence complementarity. Information at the DNA ends may be lost due to the modification of broken DNA ends. This term covers instances of separate pathways, called classical (or canonical) and alternative nonhomologous end joining (C-NHEJ and A-NHEJ). These in turn may further branch into sub-pathways, but evidence is still unclear.
histone exchange The replacement, within chromatin, of resident histones or histone subunits with alternative, sometimes variant, histones or subunits.
histone H2A K63-linked ubiquitination A histone ubiquitination process in which a polymer of ubiquitin, formed by linkages between lysine residues at position 63 of the ubiquitin monomers, is added to a lysine residue in histone H2A or the variant H2AX.
histone H2A ubiquitination The modification of histone H2A by addition of one or more ubiquitin groups.
histone H2B ubiquitination The modification of histone H2B by addition of ubiquitin groups.
isotype switching The switching of activated B cells from IgM biosynthesis to biosynthesis of other isotypes of immunoglobulin, accomplished through a recombination process involving an intrachromosomal deletion involving switch regions that reside 5' of each constant region gene segment in the immunoglobulin heavy chain locus.
negative regulation of transcription elongation by RNA polymerase II Any process that stops, prevents, or reduces the frequency, rate or extent of transcription elongation, the extension of an RNA molecule after transcription initiation and promoter clearance by the addition of ribonucleotides, catalyzed by RNA polymerase II.
positive regulation of DNA repair Any process that activates or increases the frequency, rate or extent of DNA repair.
protein K48-linked ubiquitination A protein ubiquitination process in which a polymer of ubiquitin, formed by linkages between lysine residues at position 48 of the ubiquitin monomers, is added to a protein. K48-linked ubiquitination targets the substrate protein for degradation.
protein K63-linked ubiquitination A protein ubiquitination process in which a polymer of ubiquitin, formed by linkages between lysine residues at position 63 of the ubiquitin monomers, is added to a protein. K63-linked ubiquitination does not target the substrate protein for degradation, but is involved in several pathways, notably as a signal to promote error-free DNA postreplication repair.
response to ionizing radiation Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a ionizing radiation stimulus. Ionizing radiation is radiation with sufficient energy to remove electrons from atoms and may arise from spontaneous decay of unstable isotopes, resulting in alpha and beta particles and gamma rays. Ionizing radiation also includes X-rays.
spermatid development The process whose specific outcome is the progression of a spermatid over time, from its formation to the mature structure.
ubiquitin-dependent protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MGEPGFFVTG DRAGGRSWCL RRVGMSAGWL LLEDGCEVTV GRGFGVTYQL VSKICPLMIS
70 80 90 100 110 120
RNHCVLKQNP EGQWTIMDNK SLNGVWLNRA RLEPLSVYSI HQGDYIQLGV PLENKENAEY
130 140 150 160 170 180
EYEVTEEDWE TIYPCLSPKN DQVIEKNKEL RTKRKFSLDE LAGPGAEGPS NLKSKINKVS
190 200 210 220 230 240
CESGQSVKSQ GKGEVSSTPS ENLDPKLTVL EPSKKTTGAP IYLGFPKVTE VHHEQTASNS
250 260 270 280 290 300
SASQRGLQMF KVTMSRILRL KIQMQEKHEA VMNVKKQTQK GNSKKIVQME QELLDLQSQL
310 320 330 340 350 360
CAEQAQQQAR VEQLEKTFQE EEQHLQDLEI AQGEEDLRQQ LAQALQEHWA LMEELNRSKK
370 380 390 400 410 420
DFEAIIQAKN KELEQTKEEK EKVQAQKEEV LSHMNDVLEN ELQCIICSEY FIEAVTLNCA
430 440 450 460 470 480
HSFCSYCINE WMKRKIECPI CRKDIKSKTY SLVLDNCINK MVNNLSSEVK ERRIVLIRER
KVKRLF