Q3UMW7

SS
Gene name
Mapkapk3
Protein name
MAP kinase-activated protein kinase 3
Names
MAPK-activated protein kinase 3 , MAPKAP kinase 3 , MAPKAP-K3 , MAPKAPK-3 , MK-3 , EC 2.7.11.1
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:102626
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

MAPKAPK2 is a stress-activated serine/threonine-protein kinase. MAPKAPK2, contains an autoinhibitory α-helix overlapping with a leucine-rich nuclear export signal (NES), and also lies close to the nuclear localization signal (NLS). The closed conformation of MAPKAPK2 prevents exportin binding to NES. Phosphorylation of MAPKAPK2 at threonine 317 induces a conformational change, thus unmasks and increases the activity of NES.

Autoinhibitory domains (AIDs)

313-333 (Autoinhibitory A-helix) SS

Target domain

5-306 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q3UMW7

Entry ID Method Resolution Chain Position Source
AF-Q3UMW7-F1 Predicted AlphaFoldDB

31 variants for Q3UMW7

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3389050827 15 P>L No EVA
rs3389062874 50 Q>* No EVA
rs3389072344 50 Q>R No EVA
rs3389072522 52 L>M No EVA
rs3389066725 53 G>V No EVA
rs3389075634 54 L>Q No EVA
rs3389016184 54 L>V No EVA
rs3389045869 57 N>D No EVA
rs3389073499 57 N>K No EVA
rs3389062831 59 K>* No EVA
rs3389045828 59 K>T No EVA
rs3389050779 98 H>R No EVA
rs3389050793 114 R>H No EVA
rs3389037430 119 V>I No EVA
rs3389072297 135 R>C No EVA
rs3389062890 142 E>D No EVA
rs3400083064 144 E>A No EVA
rs3400649742 144 E>D No EVA
rs3400550253 145 A>S No EVA
rs3389066741 147 E>K No EVA
rs3400103961 160 H>L No EVA
rs3389066761 165 A>T No EVA
rs3389058698 169 V>I No EVA
rs29646363 189 D>G No EVA
rs3389069317 212 A>T No EVA
rs3400649752 231 G>C No EVA
rs3389079174 303 H>N No EVA
rs3389079195 314 Q>H No EVA
rs3389072532 354 K>* No EVA
rs3389072306 370 K>* No EVA
rs3389069525 376 S>P No EVA

No associated diseases with Q3UMW7

109 regional properties for Q3UMW7

Type Name Position InterPro Accession
ptm EGF-type aspartate/asparagine hydroxylation site 198 - 209 IPR000152-1
ptm EGF-type aspartate/asparagine hydroxylation site 275 - 286 IPR000152-2
ptm EGF-type aspartate/asparagine hydroxylation site 315 - 326 IPR000152-3
ptm EGF-type aspartate/asparagine hydroxylation site 353 - 364 IPR000152-4
ptm EGF-type aspartate/asparagine hydroxylation site 433 - 444 IPR000152-5
ptm EGF-type aspartate/asparagine hydroxylation site 471 - 482 IPR000152-6
ptm EGF-type aspartate/asparagine hydroxylation site 509 - 520 IPR000152-7
ptm EGF-type aspartate/asparagine hydroxylation site 547 - 558 IPR000152-8
ptm EGF-type aspartate/asparagine hydroxylation site 584 - 595 IPR000152-9
ptm EGF-type aspartate/asparagine hydroxylation site 622 - 633 IPR000152-10
ptm EGF-type aspartate/asparagine hydroxylation site 659 - 670 IPR000152-11
ptm EGF-type aspartate/asparagine hydroxylation site 697 - 708 IPR000152-12
ptm EGF-type aspartate/asparagine hydroxylation site 772 - 783 IPR000152-13
ptm EGF-type aspartate/asparagine hydroxylation site 810 - 821 IPR000152-14
ptm EGF-type aspartate/asparagine hydroxylation site 888 - 899 IPR000152-15
ptm EGF-type aspartate/asparagine hydroxylation site 926 - 937 IPR000152-16
ptm EGF-type aspartate/asparagine hydroxylation site 964 - 975 IPR000152-17
ptm EGF-type aspartate/asparagine hydroxylation site 1002 - 1013 IPR000152-18
ptm EGF-type aspartate/asparagine hydroxylation site 1040 - 1051 IPR000152-19
ptm EGF-type aspartate/asparagine hydroxylation site 1164 - 1175 IPR000152-20
ptm EGF-type aspartate/asparagine hydroxylation site 1202 - 1213 IPR000152-21
ptm EGF-type aspartate/asparagine hydroxylation site 1241 - 1252 IPR000152-22
domain EGF-like domain 24 - 63 IPR000742-1
domain EGF-like domain 64 - 102 IPR000742-2
domain EGF-like domain 105 - 143 IPR000742-3
domain EGF-like domain 144 - 180 IPR000742-4
domain EGF-like domain 182 - 219 IPR000742-5
domain EGF-like domain 221 - 258 IPR000742-6
domain EGF-like domain 260 - 296 IPR000742-7
domain EGF-like domain 298 - 336 IPR000742-8
domain EGF-like domain 338 - 374 IPR000742-9
domain EGF-like domain 375 - 413 IPR000742-10
domain EGF-like domain 415 - 454 IPR000742-11
domain EGF-like domain 456 - 492 IPR000742-12
domain EGF-like domain 494 - 530 IPR000742-13
domain EGF-like domain 532 - 568 IPR000742-14
domain EGF-like domain 570 - 605 IPR000742-15
domain EGF-like domain 607 - 643 IPR000742-16
domain EGF-like domain 645 - 680 IPR000742-17
domain EGF-like domain 682 - 718 IPR000742-18
domain EGF-like domain 720 - 755 IPR000742-19
domain EGF-like domain 757 - 793 IPR000742-20
domain EGF-like domain 795 - 831 IPR000742-21
domain EGF-like domain 833 - 871 IPR000742-22
domain EGF-like domain 873 - 909 IPR000742-23
domain EGF-like domain 911 - 947 IPR000742-24
domain EGF-like domain 949 - 985 IPR000742-25
domain EGF-like domain 987 - 1023 IPR000742-26
domain EGF-like domain 1025 - 1061 IPR000742-27
domain EGF-like domain 1063 - 1099 IPR000742-28
domain EGF-like domain 1104 - 1147 IPR000742-29
domain EGF-like domain 1149 - 1185 IPR000742-30
domain EGF-like domain 1187 - 1223 IPR000742-31
domain EGF-like domain 1225 - 1262 IPR000742-32
domain EGF-like domain 1264 - 1302 IPR000742-33
domain EGF-like domain 1304 - 1343 IPR000742-34
domain EGF-like domain 1375 - 1412 IPR000742-35
domain Notch domain 1418 - 1544 IPR000800
domain EGF-like calcium-binding domain 64 - 102 IPR001881-1
domain EGF-like calcium-binding domain 109 - 143 IPR001881-2
domain EGF-like calcium-binding domain 146 - 180 IPR001881-3
domain EGF-like calcium-binding domain 182 - 219 IPR001881-4
domain EGF-like calcium-binding domain 225 - 258 IPR001881-5
domain EGF-like calcium-binding domain 260 - 296 IPR001881-6
domain EGF-like calcium-binding domain 298 - 336 IPR001881-7
domain EGF-like calcium-binding domain 338 - 374 IPR001881-8
domain EGF-like calcium-binding domain 415 - 454 IPR001881-9
domain EGF-like calcium-binding domain 456 - 492 IPR001881-10
domain EGF-like calcium-binding domain 494 - 530 IPR001881-11
domain EGF-like calcium-binding domain 532 - 568 IPR001881-12
domain EGF-like calcium-binding domain 570 - 605 IPR001881-13
domain EGF-like calcium-binding domain 607 - 643 IPR001881-14
domain EGF-like calcium-binding domain 645 - 680 IPR001881-15
domain EGF-like calcium-binding domain 682 - 718 IPR001881-16
domain EGF-like calcium-binding domain 720 - 755 IPR001881-17
domain EGF-like calcium-binding domain 757 - 793 IPR001881-18
domain EGF-like calcium-binding domain 795 - 831 IPR001881-19
domain EGF-like calcium-binding domain 837 - 871 IPR001881-20
domain EGF-like calcium-binding domain 873 - 909 IPR001881-21
domain EGF-like calcium-binding domain 911 - 947 IPR001881-22
domain EGF-like calcium-binding domain 949 - 985 IPR001881-23
domain EGF-like calcium-binding domain 987 - 1023 IPR001881-24
domain EGF-like calcium-binding domain 1025 - 1061 IPR001881-25
domain EGF-like calcium-binding domain 1064 - 1099 IPR001881-26
domain EGF-like calcium-binding domain 1105 - 1147 IPR001881-27
domain EGF-like calcium-binding domain 1149 - 1185 IPR001881-28
domain EGF-like calcium-binding domain 1187 - 1223 IPR001881-29
domain EGF-like calcium-binding domain 1225 - 1262 IPR001881-30
domain EGF-like calcium-binding domain 1264 - 1302 IPR001881-31
domain EGF-like calcium-binding domain 1306 - 1343 IPR001881-32
domain EGF-like calcium-binding domain 1370 - 1412 IPR001881-33
repeat Ankyrin repeat 1827 - 1939 IPR002110-1
repeat Ankyrin repeat 1943 - 2041 IPR002110-2
domain Notch, NOD domain 1539 - 1595 IPR010660
domain Notch, NODP domain 1617 - 1677 IPR011656
conserved_site EGF-like, conserved site 230 - 252 IPR013032-1
conserved_site EGF-like, conserved site 347 - 367 IPR013032-2
conserved_site EGF-like, conserved site 579 - 599 IPR013032-3
conserved_site EGF-like, conserved site 729 - 749 IPR013032-4
conserved_site EGF-like calcium-binding, conserved site 182 - 207 IPR018097-1
conserved_site EGF-like calcium-binding, conserved site 260 - 284 IPR018097-2
conserved_site EGF-like calcium-binding, conserved site 456 - 480 IPR018097-3
conserved_site EGF-like calcium-binding, conserved site 532 - 556 IPR018097-4
conserved_site EGF-like calcium-binding, conserved site 682 - 706 IPR018097-5
conserved_site EGF-like calcium-binding, conserved site 911 - 935 IPR018097-6
conserved_site EGF-like calcium-binding, conserved site 1025 - 1049 IPR018097-7
conserved_site EGF-like calcium-binding, conserved site 1149 - 1173 IPR018097-8
conserved_site EGF-like calcium-binding, conserved site 1225 - 1250 IPR018097-9
domain Notch, C-terminal 2380 - 2445 IPR024600

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • [Isoform 1]: Nucleus
  • Cytoplasm
  • Predominantly located in the nucleus, when activated it translocates to the cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

7 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calcium-dependent protein serine/threonine kinase activity Calcium-dependent catalysis of the reactions
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
calmodulin-dependent protein kinase activity Calmodulin-dependent catalysis of the reactions
mitogen-activated protein kinase binding Binding to a mitogen-activated protein kinase.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

6 GO annotations of biological process

Name Definition
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
macropinocytosis An endocytosis process that results in the uptake of liquid material by cells from their external environment by the 'ruffling' of the cell membrane to form heterogeneously sized intracellular vesicles called macropinosomes, which can be up to 5 micrometers in size.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
response to cytokine Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cytokine stimulus.
response to lipopolysaccharide Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria.
toll-like receptor signaling pathway The series of molecular signals initiated by a ligand binding to a toll-like receptor of a target cell. Toll-like receptors directly bind pattern motifs from a variety of microbial sources to initiate an innate immune response.

12 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q3SYZ2 MAPKAPK3 MAP kinase-activated protein kinase 3 Bos taurus (Bovine) SS
P49071 MAPk-Ak2 MAP kinase-activated protein kinase 2 Drosophila melanogaster (Fruit fly) SS
P49137 MAPKAPK2 MAP kinase-activated protein kinase 2 Homo sapiens (Human) EV
Q8IW41 MAPKAPK5 MAP kinase-activated protein kinase 5 Homo sapiens (Human) SS
Q16644 MAPKAPK3 MAP kinase-activated protein kinase 3 Homo sapiens (Human) SS
O54992 Mapkapk5 MAP kinase-activated protein kinase 5 Mus musculus (Mouse) EV
P49138 Mapkapk2 MAP kinase-activated protein kinase 2 Mus musculus (Mouse) EV
O08605 Mknk1 MAP kinase-interacting serine/threonine-protein kinase 1 Mus musculus (Mouse) PR
Q8CDB0 Mknk2 MAP kinase-interacting serine/threonine-protein kinase 2 Mus musculus (Mouse) PR
P08414 Camk4 Calcium/calmodulin-dependent protein kinase type IV Mus musculus (Mouse) SS
Q66H84 Mapkapk3 MAP kinase-activated protein kinase 3 Rattus norvegicus (Rat) SS
Q965G5 mak-2 MAP kinase-activated protein kinase mak-2 Caenorhabditis elegans SS
10 20 30 40 50 60
MDGETAGEKG SLVPPPGALG GSALGGAPAP GVRREPKKYA VTDDYQLSKQ VLGLGVNGKV
70 80 90 100 110 120
LECYHRRSGQ KCALKLLYDS PKARQEVDHH WQASGGPHIV RILDVYENMH HGKRCLLIVM
130 140 150 160 170 180
ECMEGGELFS RIQERGDQAF TEREAAEIMR DIGTAIQFLH SRNIAHRDVK PENLLYTSKE
190 200 210 220 230 240
KDAVLKLTDF GFAKETTQNA LQTPCYTPYY VAPEVLGPEK YDKSCDMWSL GVIMYILLCG
250 260 270 280 290 300
FPPFYSNTGQ AISPGMKRRI RLGQYSFPNP EWLDVSEDAK QLIRLLLKTD PTERLTIMQF
310 320 330 340 350 360
MNHPWINQSM VVPQTPLYTA RVLQEDKDHW DDVKEEMTSA LATMRVDYDQ VKIKDLKTSN
370 380
NRLLNKRRKK QAGSSSASQG CNNQ