Q27828

PR
Gene name
GSPATT00019973001
Protein name
Bifunctional dihydrofolate reductase-thymidylate synthase
Names
DHFR-TS
Species
Paramecium tetraurelia
KEGG Pathway
ptm:GSPATT00019973001
EC number
1.5.1.3: With NAD(+) or NADP(+) as acceptor
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

21-99 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q27828

Entry ID Method Resolution Chain Position Source
AF-Q27828-F1 Predicted AlphaFoldDB

No variants for Q27828

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q27828

No associated diseases with Q27828

4 regional properties for Q27828

Type Name Position InterPro Accession
domain Dihydrofolate reductase domain 6 - 165 IPR001796
conserved_site Dihydrofolate reductase conserved site 18 - 40 IPR017925
active_site Thymidylate synthase, active site 325 - 353 IPR020940
domain Thymidylate synthase/dCMP hydroxymethylase domain 181 - 462 IPR023451

Functions

Description
EC Number 1.5.1.3 With NAD(+) or NADP(+) as acceptor
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

2 GO annotations of molecular function

Name Definition
dihydrofolate reductase activity Catalysis of the reaction: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+.
thymidylate synthase activity Catalysis of the reaction: 5,10-methylenetetrahydrofolate + dUMP = 7,8-dihydrofolate + thymidylate.

4 GO annotations of biological process

Name Definition
dTMP biosynthetic process The chemical reactions and pathways resulting in the formation of dTMP, deoxyribosylthymine monophosphate (2'-deoxyribosylthymine 5'-phosphate).
methylation The process in which a methyl group is covalently attached to a molecule.
one-carbon metabolic process The chemical reactions and pathways involving the transfer of one-carbon units in various oxidation states.
tetrahydrofolate biosynthetic process The chemical reactions and pathways resulting in the formation of tetrahydrofolate, 5,6,7,8-tetrahydrofolic acid, a folate derivative bearing additional hydrogens on the pterin group.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MTTNKTFSMV LAMTLNGGIG YQNRLPWKLK EDLQRFKKIT TGGIVIMGRK TFESMNSKPL
70 80 90 100 110 120
PNRVNVVISK NMKSSNEVQV FPRIEDALQF YNTSHQKLYL IGGKRIFEEG LATDKCSDVH
130 140 150 160 170 180
LTRIGVETKC DVYLNKNIFS TFQVNKTSQT KSENGINYDY QHLINKNSHE QSYIDEEHQE
190 200 210 220 230 240
NQYLDMITKI MKEGVSKDDR TGVGTMSIFG QTMRFNLAQS FPLLTTKKVF FRGVVEELLW
250 260 270 280 290 300
FLRGNTNGKL LLDKGVKIWE GNGTREYLDT IGLQHRQEHD LGPVYGFQWR HFGAKYKDCQ
310 320 330 340 350 360
TDYSNQGVDQ VKEIIQLLKN NPDSRRIILS AWNPIDLKQM ALPPCHVMSQ FFVANGKLSC
370 380 390 400 410 420
MMYQRSCDFG LGIPFNIASY ALLTYMLAKE CNLNLGEFVH VLGDTHIYSN HVEALKKQIE
430 440 450 460
RVPYPFPLLK IKGNKSLFDY TYEDFELVGY NAHDKIEMKM AV