AiPD: Autoinhibited Protein Database

Q16514

Gene name	TAF12
Protein name	Transcription initiation factor TFIID subunit 12
Names	Transcription initiation factor TFIID 20/15 kDa subunits, TAFII-20/TAFII-15, TAFII20/TAFII15
Species	Homo sapiens (Human)
KEGG Pathway	hsa:6883
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

11-86 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

11-86 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

34 structures for Q16514

Entry ID	Method	Resolution	Chain	Position	Source
1H3O	X-ray	230 A	B/D	57-128	PDB
6MZC	EM	450 A	R	1-161	PDB
6MZD	EM	980 A	Q	1-161	PDB
6MZL	EM	2300 A	Q/R	1-161	PDB
6MZM	EM	750 A	R	1-161	PDB
7EDX	EM	450 A	L/l	1-161	PDB
7EG7	EM	620 A	L/l	1-161	PDB
7EG8	EM	740 A	L/l	1-161	PDB
7EG9	EM	370 A	L/l	1-161	PDB
7EGA	EM	410 A	L/l	1-161	PDB
7EGB	EM	330 A	L/l	1-161	PDB
7EGC	EM	390 A	L/l	1-161	PDB
7EGD	EM	675 A	L/l	1-161	PDB
7EGE	EM	900 A	L/l	1-161	PDB
7EGF	EM	316 A	l	1-161	PDB
7EGG	EM	277 A	L	1-161	PDB
7EGI	EM	982 A	L/l	1-161	PDB
7EGJ	EM	864 A	L/l	1-161	PDB
7ENA	EM	407 A	DL/Dl	1-161	PDB
7ENC	EM	413 A	DL/Dl	1-161	PDB
7KTR	EM	293 A	G	1-161	PDB
7KTS	EM	1909 A	G	1-161	PDB
8GXQ	EM	504 A	DL/Dl	1-161	PDB
8GXS	EM	416 A	DL/Dl	1-161	PDB
8H7G	EM	370 A	R	1-161	PDB
8WAK	EM	547 A	L/l	1-161	PDB
8WAL	EM	852 A	L/l	1-161	PDB
8WAN	EM	607 A	L/l	1-161	PDB
8WAO	EM	640 A	L/l	1-161	PDB
8WAP	EM	585 A	L/l	1-161	PDB
8WAQ	EM	629 A	L/l	1-161	PDB
8WAR	EM	720 A	L/l	1-161	PDB
8WAS	EM	613 A	L/l	1-161	PDB
AF-Q16514-F1	Predicted				AlphaFoldDB

92 variants for Q16514

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs375818698 CA19979568	2	N>D		No	ClinGen ESP
CA723058 rs778342112	2	N>K		No	ClinGen ExAC gnomAD
TCGA novel	2	N>Y	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA19979542 rs932515133	3	Q>H		No	ClinGen TOPMed
rs756805497 CA723057	3	Q>P		No	ClinGen ExAC TOPMed gnomAD
rs11540607 CA19979541	4	F>S		No	ClinGen TOPMed
CA19979532 rs796797289	6	P>L		No	ClinGen Ensembl
rs867724132 CA19979517	11	N>S		No	ClinGen gnomAD
rs138962568 CA723053	14	N>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA723052 rs752379232	15	F>S		No	ClinGen ExAC gnomAD
rs1195675494 CA339513958	18	I>L		No	ClinGen TOPMed
CA723051 rs767427390	18	I>T		No	ClinGen ExAC TOPMed gnomAD
rs1570314658 CA339513947	19	K>N		No	ClinGen Ensembl
CA723050 rs759507763	20	P>L		No	ClinGen ExAC gnomAD
rs1570314636 CA339513934	21	E>D		No	ClinGen Ensembl
CA339513927 rs1223752034	22	P>Q		No	ClinGen TOPMed
CA339513932 rs1483775519	22	P>T		No	ClinGen gnomAD
CA723048 rs765386621	23	A>G		No	ClinGen ExAC TOPMed gnomAD
rs1312407716 CA339513912	25	T>A		No	ClinGen TOPMed
CA339513902 rs1306123502	26	P>L		No	ClinGen gnomAD
TCGA novel	26	P>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA339513901 rs1272230005	27	P>S		No	ClinGen gnomAD
CA339513876 rs1570314587	30	S>F		No	ClinGen Ensembl
CA19979461 rs1804642	31	M>I		No	ClinGen Ensembl
CA339513874 rs1241899287	31	M>V		No	ClinGen TOPMed
CA723046 rs776898097	32	A>T		No	ClinGen ExAC gnomAD
CA723045 rs536479244	33	N>H		No	ClinGen 1000Genomes ExAC gnomAD
CA723044 rs760955522	33	N>S		No	ClinGen ExAC TOPMed gnomAD
rs1411704002 CA339513851	34	S>I		No	ClinGen gnomAD
rs1371295772 CA339513841	36	A>S		No	ClinGen gnomAD
TCGA novel	36	A>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA723043 rs190752579	37	V>M		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs372852548 CA19979442	38	V>A		No	ClinGen ESP TOPMed gnomAD
rs772446054 CA723042	38	V>I		No	ClinGen ExAC gnomAD
rs376623498 CA723041	40	I>T		No	ClinGen ESP ExAC TOPMed gnomAD
rs779433369 CA339513812	41	P>A		No	ClinGen ExAC TOPMed gnomAD
rs779433369 CA723040	41	P>S		No	ClinGen ExAC TOPMed gnomAD
rs777359513 COSM1341597 CA723037	45	G>E	Variant assessed as Somatic; 0.0 impact. large_intestine [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ExAC NCI-TCGA gnomAD
rs748810993 CA723038	45	G>R		No	ClinGen ExAC gnomAD
rs755657743 CA723036	46	A>P		No	ClinGen ExAC gnomAD
CA19979409 rs755657743	46	A>S		No	ClinGen ExAC gnomAD
rs781086540 CA723034	49	R>C		No	ClinGen ExAC TOPMed gnomAD
CA723033 rs371905728 COSM380739	49	R>H	lung [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA339513737 rs1334655428	54	N>D		No	ClinGen gnomAD
CA19979400 rs200737548	55	N>H		No	ClinGen 1000Genomes
CA723013 rs779903068	59	T>S		No	ClinGen ExAC TOPMed gnomAD
CA339513673 rs1251927318	61	K>R		No	ClinGen gnomAD
TCGA novel	65	D>E	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	65	D>Y	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA723010 rs764054566	66	L>S		No	ClinGen ExAC gnomAD
rs569037333 CA339513612	69	E>D		No	ClinGen 1000Genomes ExAC gnomAD
CA723008 rs752907499	71	D>G		No	ClinGen ExAC gnomAD
CA339513598 rs1450967429	72	P>T		No	ClinGen gnomAD
rs767780832 CA723007	73	N>S		No	ClinGen ExAC gnomAD
rs1277009911 CA339513428	93	E>G		No	ClinGen gnomAD
CA19975494 rs752653157	93	E>K		No	ClinGen gnomAD
rs957391314 CA19975490	94	S>N		No	ClinGen TOPMed
rs1339823666 CA339513419	94	S>R		No	ClinGen TOPMed gnomAD
rs755154546 CA722987	97	T>I		No	ClinGen ExAC gnomAD
rs1557459847 CA339513388	100	C>R		No	ClinGen Ensembl
CA339513371 rs1444472049	102	L>F		No	ClinGen TOPMed
rs1224981938 CA339513362	103	A>V		No	ClinGen gnomAD
rs1035712910 CA19975463	104	R>Q		No	ClinGen TOPMed gnomAD
rs998942474 CA19975486	104	R>W		No	ClinGen gnomAD
rs1373155792 CA339513346	106	R>H		No	ClinGen gnomAD
CA339513343 rs1309474839	107	K>E		No	ClinGen gnomAD
CA339513326 rs1410119848	109	S>T		No	ClinGen gnomAD
rs766632265 CA722985	110	T>I		No	ClinGen ExAC gnomAD
rs1419173479 CA339513295	114	K>T		No	ClinGen gnomAD
CA19975452 rs760116334	118	L>V		No	ClinGen TOPMed gnomAD
CA339513255 rs1490803647	120	L>V		No	ClinGen TOPMed
rs1203305008 CA339513182	122	R>C	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
rs376483218 CA722970 COSM3789857	122	R>H	Variant assessed as Somatic; 0.0 impact. urinary_tract [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ESP ExAC NCI-TCGA TOPMed gnomAD
CA722968 rs751701996	123	Q>L		No	ClinGen ExAC gnomAD
rs758676855 CA722966	126	M>L		No	ClinGen ExAC TOPMed gnomAD
rs1332530992 CA339513060	132	G>V		No	ClinGen gnomAD
CA19972468 rs898769916	133	S>C		No	ClinGen TOPMed
CA339513014 rs1349392976	136	I>V		No	ClinGen gnomAD
CA339512996 rs191329207	137	R>P		No	ClinGen 1000Genomes ExAC gnomAD
rs191329207 CA722964	137	R>Q		No	ClinGen 1000Genomes ExAC gnomAD
CA339512907 rs1325580550	144	T>A		No	ClinGen gnomAD
rs144276665 CA722961	145	T>A		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA339512889 rs1391417783	145	T>I		No	ClinGen gnomAD
CA722962 rs144276665	145	T>S		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA722959 rs774987809	147	A>D		No	ClinGen ExAC gnomAD
rs760325663 CA722960	147	A>P		No	ClinGen ExAC TOPMed gnomAD
rs1405973146 CA339512843	149	K>R		No	ClinGen TOPMed gnomAD
CA19971850 rs139249390	151	R>K		No	ClinGen ESP TOPMed
rs766970602 CA722940	152	M>T		No	ClinGen ExAC gnomAD
rs1432121046 CA339512721	155	I>M		No	ClinGen TOPMed
CA339512717 rs1214377732	156	R>W		No	ClinGen gnomAD
rs759182275 CA722939	159	T>A		No	ClinGen ExAC gnomAD

No associated diseases with Q16514

1 regional properties for Q16514

Type	Name	Position	InterPro Accession
domain	Transcription initiation factor TFIID subunit 12 domain	58 - 126	IPR003228

Functions

		Description
EC Number
Subcellular Localization	Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

6 GO annotations of cellular component

Name	Definition
nucleoplasm	That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
SAGA complex	A SAGA-type histone acetyltransferase complex that deubiquitinates H2A and/or H2B. This complex is organized into several functional submodules: a structural core including the activator binding module and consisting of ADA1 or a homolog, members of the SPT and TAF protein families as well as promotor recruitment factor TRRAP/TRA1, a histone acetyltransferase (HAT) module consisting of GCN5/KAT2A or PCAF/KAT2B, ADA2, ADA3/NGG1, and SGF29 or homologues thereof, a histone deubiquitinase (DUB) module consisting of ATXN7/SGF73, ATXN7L3/SGF11, ENY2/SUS1 and USP22/UBP8 or homologues thereof, and in some taxa a splicing module consisting of SF3B3 and SF3B5 or homologues thereof (not in fungi). In budding yeast also contains Spt8 which distinguishes it from SAGA-like (SLIK) complex (GO:0046695).
SLIK (SAGA-like) complex	A SAGA-type histone acetyltransferase complex that contains a smaller form of Spt7 (lacking the SPT8 binding region) than the fungal SAGA complex, and consequently lacks Spt8. The complex is involved in the yeast retrograde response pathway, which is important for gene expression changes during mitochondrial dysfunction.
transcription factor TFIID complex	A complex composed of TATA binding protein (TBP) and TBP associated factors (TAFs); the total mass is typically about 800 kDa. Most of the TAFs are conserved across species. In TATA-containing promoters for RNA polymerase II (Pol II), TFIID is believed to recognize at least two distinct elements, the TATA element and a downstream promoter element. TFIID is also involved in recognition of TATA-less Pol II promoters. Binding of TFIID to DNA is necessary but not sufficient for transcription initiation from most RNA polymerase II promoters.
transcription factor TFTC complex	A protein complex that does not contain either a TATA-binding protein (TBP) or a TBP-like factor, but is composed of several TAFIIs and other proteins, including a histone acetyltransferase. This complex is able to nucleate transcription initiation by RNA polymerase II, can mediate transcriptional activation, and has histone acetyltransferase activity.

5 GO annotations of molecular function

Name	Definition
DNA binding	Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA-binding transcription factor binding	Binding to a DNA-binding transcription factor, a protein that interacts with a specific DNA sequence (sometimes referred to as a motif) within the regulatory region of a gene to modulate transcription.
protein heterodimerization activity	Binding to a nonidentical protein to form a heterodimer.
TBP-class protein binding	Binding to a member of the class of TATA-binding proteins (TBP), including any of the TBP-related factors (TRFs).
transcription coactivator activity	A transcription coregulator activity that activates or increases the transcription of specific gene sets via binding to a DNA-bound DNA-binding transcription factor, either on its own or as part of a complex. Coactivators often act by altering chromatin structure and modifications. For example, one class of transcription coactivators modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators. A fourth class of coactivator activity is the bridging of a DNA-binding transcription factor to the general (basal) transcription machinery. The Mediator complex, which bridges sequence-specific DNA binding transcription factors and RNA polymerase, is also a transcription coactivator.

18 GO annotations of biological process

Name	Definition
DNA-templated transcription initiation	The initial step of transcription, consisting of the assembly of the RNA polymerase preinitiation complex (PIC) at a gene promoter, as well as the formation of the first few bonds of the RNA transcript. Transcription initiation includes abortive initiation events, which occur when the first few nucleotides are repeatedly synthesized and then released, and ends when promoter clearance takes place.
histone acetylation	The modification of a histone by the addition of an acetyl group.
histone H3 acetylation	The modification of histone H3 by the addition of an acetyl group.
monoubiquitinated histone deubiquitination	The removal of the ubiquitin group from a monoubiquitinated histone protein.
monoubiquitinated histone H2A deubiquitination	The removal of the ubiquitin group from a monoubiquitinated histone H2A protein.
mRNA transcription by RNA polymerase II	The cellular synthesis of messenger RNA (mRNA) from a DNA template by RNA polymerase II, originating at an RNA polymerase II promoter.
positive regulation of DNA-binding transcription factor activity	Any process that activates or increases the frequency, rate or extent of activity of a transcription factor, any factor involved in the initiation or regulation of transcription.
positive regulation of DNA-templated transcription	Any process that activates or increases the frequency, rate or extent of cellular DNA-templated transcription.
positive regulation of transcription by RNA polymerase II	Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
positive regulation of transcription initiation by RNA polymerase II	Any process that increases the rate, frequency or extent of a process involved in starting transcription from an RNA polymerase II promoter.
protein phosphorylation	The process of introducing a phosphate group on to a protein.
regulation of DNA repair	Any process that modulates the frequency, rate or extent of DNA repair.
regulation of DNA-templated transcription	Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
regulation of RNA splicing	Any process that modulates the frequency, rate or extent of RNA splicing, the process of removing sections of the primary RNA transcript to remove sequences not present in the mature form of the RNA and joining the remaining sections to form the mature form of the RNA.
regulation of transcription by RNA polymerase II	Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
RNA polymerase II preinitiation complex assembly	The aggregation, arrangement and bonding together of proteins on an RNA polymerase II promoter DNA to form the transcriptional preinitiation complex (PIC), the formation of which is a prerequisite for transcription by RNA polymerase.
transcription by RNA polymerase II	The synthesis of RNA from a DNA template by RNA polymerase II (RNAP II), originating at an RNA polymerase II promoter. Includes transcription of messenger RNA (mRNA) and certain small nuclear RNAs (snRNAs).
transcription initiation at RNA polymerase II promoter	A transcription initiation process that takes place at a RNA polymerase II gene promoter. Messenger RNAs (mRNA) genes, as well as some non-coding RNAs, are transcribed by RNA polymerase II.

No homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
No homologous proteins

10	20	30	40	50	60
MNQFGPSALI	NLSNFSSIKP	EPASTPPQGS	MANSTAVVKI	PGTPGAGGRL	SPENNQVLTK
70	80	90	100	110	120
KKLQDLVREV	DPNEQLDEDV	EEMLLQIADD	FIESVVTAAC	QLARHRKSST	LEVKDVQLHL
130	140	150	160
ERQWNMWIPG	FGSEEIRPYK	KACTTEAHKQ	RMALIRKTTK	K