Q02554

PR
Gene name
CUS1 (YMR240C, YM9408.02C)
Protein name
Cold sensitive U2 snRNA suppressor 1
Names
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YMR240C
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-259 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

10 structures for Q02554

Entry ID Method Resolution Chain Position Source
5GM6 EM 350 A H 1-436 PDB
5LSB X-ray 270 A B/D/H 290-368 PDB
5LSL X-ray 165 A E/F/G/H 290-368 PDB
5NRL EM 720 A Q 1-435 PDB
5ZWM EM 340 A 2 1-436 PDB
5ZWO EM 390 A 2 1-436 PDB
6G90 EM 400 A Q 1-435 PDB
7OQB EM 900 A Q 1-436 PDB
7OQE EM 590 A Q 1-436 PDB
AF-Q02554-F1 Predicted AlphaFoldDB

9 variants for Q02554

Variant ID(s) Position Change Description Diseaes Association Provenance
s13-751107 45 T>A No SGRP
s13-751100 47 S>N No SGRP
s13-751074 56 D>N No SGRP
s13-751062 60 E>K No SGRP
s13-750980 87 N>S No SGRP
181 E>K allele CUS1-54; cold-sensitive growth suppressor [UniProt] No
s13-750176 355 R>K No SGRP
s13-750174 356 S>P No SGRP
s13-750161 360 G>A No SGRP

No associated diseases with Q02554

2 regional properties for Q02554

Type Name Position InterPro Accession
domain PSP, proline-rich 286 - 339 IPR006568
domain Domain of unknown function DUF382 143 - 275 IPR007180

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
catalytic step 2 spliceosome A spliceosomal complex that contains three snRNPs, including U5, bound to a splicing intermediate in which the first catalytic cleavage of the 5' splice site has occurred. The precise subunit composition differs significantly from that of the catalytic step 1, or activated, spliceosome, and includes many proteins in addition to those found in the associated snRNPs.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
precatalytic spliceosome A spliceosomal complex that is formed by the recruitment of a preassembled U5-containing tri-snRNP to the prespliceosome. Although all 5 snRNPs are present, the precatalytic spliceosome is catalytically inactive. The precatalytic spliceosome includes many proteins in addition to those found in the associated snRNPs.
spliceosomal complex Any of a series of ribonucleoprotein complexes that contain snRNA(s) and small nuclear ribonucleoproteins (snRNPs), and are formed sequentially during the spliceosomal splicing of one or more substrate RNAs, and which also contain the RNA substrate(s) from the initial target RNAs of splicing, the splicing intermediate RNA(s), to the final RNA products. During cis-splicing, the initial target RNA is a single, contiguous RNA transcript, whether mRNA, snoRNA, etc., and the released products are a spliced RNA and an excised intron, generally as a lariat structure. During trans-splicing, there are two initial substrate RNAs, the spliced leader RNA and a pre-mRNA.
U2 snRNP A ribonucleoprotein complex that contains small nuclear RNA U2, a heptameric ring of Sm proteins, as well as several proteins that are unique to the U2 snRNP, most of which remain associated with the U2 snRNA both while the U2 snRNP is free or assembled into a series of spliceosomal complexes.
U2-type prespliceosome A spliceosomal complex that is formed by association of the 5' splice site with the U1 snRNP, while the branch point sequence is recognized by the U2 snRNP. The prespliceosome includes many proteins in addition to those found in the U1 and U2 snRNPs. Commitment to a given pair of 5' and 3' splice sites occurs at the time of prespliceosome formation.
U2-type spliceosomal complex Any spliceosomal complex that forms during the splicing of a messenger RNA primary transcript to excise an intron that has canonical consensus sequences near the 5' and 3' ends.

1 GO annotations of molecular function

Name Definition
RNA binding Binding to an RNA molecule or a portion thereof.

3 GO annotations of biological process

Name Definition
mRNA splicing, via spliceosome The joining together of exons from one or more primary transcripts of messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced.
spliceosomal complex assembly The aggregation, arrangement and bonding together of a spliceosomal complex, a ribonucleoprotein apparatus that catalyzes nuclear mRNA splicing via transesterification reactions.
U2-type prespliceosome assembly The aggregation, arrangement and bonding together of a set of components to form an U2-type prespliceosome.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MARTKSRKRS GNNQNKNASV VNNKAEIAAM IDARRLEQKK KGGVTNSKGK TNKVVDAKLE
70 80 90 100 110 120
KEFKDVLQRF QVQENDTPKE ITKDEKNNHV VIVEKNPVMN RKHTAEDELE DTPSDGIEEH
130 140 150 160 170 180
LSARKRRKTE KPSLSQLKSQ VPYPQIIEWY DCDARYPGLL ASIKCTKNVI PVPSHWQSKK
190 200 210 220 230 240
EYLSGRSLLG KRPFELPDII KKTNIEQMRS TLPQSGLDGQ DEKSLKEASR ARVQPKMGAL
250 260 270 280 290 300
DLDYKKLHDV FFKIGANWKP DHLLCFGDVY YENRNLFEET NWKRMVDHKR PGRISQELRA
310 320 330 340 350 360
IMNLPEGQLP PWCMKMKDIG LPTGYPDLKI AGLNWDITNL KGDVYGKIIP NHHSRSKKQG
370 380 390 400 410 420
RNYFGALISF ETPEFENSKE DTQANAENGR QDDKIDDEVE HKLDHFQEDI SEVTSAEEKL
430
ERNEEESEKQ LYTVLK