P93163
Gene name |
GPA2 (GA2) |
Protein name |
Guanine nucleotide-binding protein alpha-2 subunit |
Names |
GP-alpha-2 |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
gmx:547977 |
EC number |
|
Protein Class |
MYOSIN (PTHR13140) |
Descriptions
Myosin Va (MyoVa) is a protein that functions as a dimer for processive walking on actin filaments. Autoinhibition of MyoVa involves the motor domain (MD) and the globular tail domain (GTD), with the motor adopting a closed, autoinhibited state that is inactive for ATPase activity. This state prevents the unnecessary consumption of ATP and uncontrolled movement when not transporting cargo. MyoVa exists in several states, including closed, half-closed, transition, and open states. The closed state of MyoVa can be changed to a half-closed state when the d-strand temporarily dissociates from the GTD, resulting in the dynamic confirmation of the short side in the triangular-shaped structure, which is called the closed state. In the presence of both a GTBM-containing protein and Rab11a, the half-closed conformation undergoes a significant motion of both the MD and the lever arm (LA), resulting in the disassembly of the hinge structure, which is referred to as a transition state. Finally, the unstable transition conformation quickly shifts to the open conformation, allowing the GTPs to tightly associate with cargo and the MDs to hydrolyze ATP for the walking process. Notably, a single cargo binding of either a GTBM-binding protein or Rab11a is insufficient to effectively activate MyoVa.
Autoinhibitory domains (AIDs)
Target domain |
193-380 (P-loop containing nucleoside triphosphate hydrolase domain) |
Relief mechanism |
Partner binding |
Assay |
|
Target domain |
198-379 (Ras-like domain) |
Relief mechanism |
Ligand binding, Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell systems, 2, 89-100
- Coleman DE et al. (1999) "Structure of Gialpha1.GppNHp, autoinhibition in a galpha protein-substrate complex", The Journal of biological chemistry, 274, 16669-72
- Lutz S et al. (2007) "Structure of Galphaq-p63RhoGEF-RhoA complex reveals a pathway for the activation of RhoA by GPCRs", Science (New York, N.Y.), 318, 1923-7
- Goricanec D et al. (2016) "Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding", Proceedings of the National Academy of Sciences of the United States of America, 113, E3629-38
Autoinhibited structure
Activated structure
1 structures for P93163
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P93163-F1 | Predicted | AlphaFoldDB |
No variants for P93163
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P93163 | |||||
No associated diseases with P93163
3 regional properties for P93163
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| conserved_site | Heat shock protein 70, conserved site | 9 - 16 | IPR018181-1 |
| conserved_site | Heat shock protein 70, conserved site | 197 - 210 | IPR018181-2 |
| conserved_site | Heat shock protein 70, conserved site | 334 - 348 | IPR018181-3 |
Functions
| Description | ||
|---|---|---|
| EC Number | ||
| Subcellular Localization |
|
|
| PANTHER Family | PTHR13140 | MYOSIN |
| PANTHER Subfamily | PTHR13140:SF273 | UNCONVENTIONAL MYOSIN-VA |
| PANTHER Protein Class | actin binding motor protein | |
| PANTHER Pathway Category |
Nicotinic acetylcholine receptor signaling pathway Myosin |
|
2 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| heterotrimeric G-protein complex | Any of a family of heterotrimeric GTP-binding and hydrolyzing proteins; they belong to a superfamily of GTPases that includes monomeric proteins such as EF-Tu and RAS. Heterotrimeric G-proteins consist of three subunits; the alpha subunit contains the guanine nucleotide binding site and possesses GTPase activity; the beta and gamma subunits are tightly associated and function as a beta-gamma heterodimer; extrinsic plasma membrane proteins (cytoplasmic face) that function as a complex to transduce signals from G protein-coupled receptors to an effector protein. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| G protein-coupled receptor binding | Binding to a G protein-coupled receptor. |
| G-protein beta/gamma-subunit complex binding | Binding to a complex of G-protein beta/gamma subunits. |
| GTP binding | Binding to GTP, guanosine triphosphate. |
| GTPase activity | Catalysis of the reaction |
| metal ion binding | Binding to a metal ion. |
1 GO annotations of biological process
| Name | Definition |
|---|---|
| adenylate cyclase-modulating G protein-coupled receptor signaling pathway | A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation or inhibition of adenylyl cyclase activity and a subsequent change in the intracellular concentration of cyclic AMP (cAMP). |
77 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P04695 | GNAT1 | Guanine nucleotide-binding protein G | Bos taurus (Bovine) | SS |
| P04696 | GNAT2 | Guanine nucleotide-binding protein G | Bos taurus (Bovine) | SS |
| P08239 | GNAO1 | Guanine nucleotide-binding protein G | Bos taurus (Bovine) | SS |
| P0C7Q4 | GNAT3 | Guanine nucleotide-binding protein G | Bos taurus (Bovine) | SS |
| P63097 | GNAI1 | Guanine nucleotide-binding protein G | Bos taurus (Bovine) | SS |
| P04896 | GNAS | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | Bos taurus (Bovine) | PR |
| P38408 | GNA14 | Guanine nucleotide-binding protein subunit alpha-14 | Bos taurus (Bovine) | PR |
| P50147 | GNAI2 | Guanine nucleotide-binding protein G | Gallus gallus (Chicken) | SS |
| P50146 | GNAI1 | Guanine nucleotide-binding protein G | Gallus gallus (Chicken) | SS |
| P38400 | GNAI2 | Guanine nucleotide-binding protein G(i) subunit alpha-2 | Canis lupus familiaris (Dog) (Canis familiaris) | PR |
| Q28294 | GNAQ | Guanine nucleotide-binding protein G(q) subunit alpha | Canis lupus familiaris (Dog) (Canis familiaris) | PR |
| P63091 | GNAS | Guanine nucleotide-binding protein G(s) subunit alpha | Canis lupus familiaris (Dog) (Canis familiaris) | PR |
| P20353 | Galphai | G protein alpha i subunit | Drosophila melanogaster (Fruit fly) | SS |
| P25157 | cta | Guanine nucleotide-binding protein subunit alpha homolog | Drosophila melanogaster (Fruit fly) | SS |
| P23625 | Galphaq | G protein alpha q subunit | Drosophila melanogaster (Fruit fly) | PR |
| P16378 | Galphao | G protein alpha o subunit | Drosophila melanogaster (Fruit fly) | SS |
| A8MTJ3 | GNAT3 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| P08754 | GNAI3 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| P11488 | GNAT1 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| P19086 | GNAZ | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| P19087 | GNAT2 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| Q14344 | GNA13 | Guanine nucleotide-binding protein subunit alpha-13 | Homo sapiens (Human) | SS |
| P29992 | GNA11 | Guanine nucleotide-binding protein subunit alpha-11 | Homo sapiens (Human) | SS |
| P30679 | GNA15 | Guanine nucleotide-binding protein subunit alpha-15 | Homo sapiens (Human) | SS |
| P50148 | GNAQ | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| P38405 | GNAL | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| O95837 | GNA14 | Guanine nucleotide-binding protein subunit alpha-14 | Homo sapiens (Human) | SS |
| P04899 | GNAI2 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| P63096 | GNAI1 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | EV |
| Q03113 | GNA12 | Guanine nucleotide-binding protein subunit alpha-12 | Homo sapiens (Human) | SS |
| P09471 | GNAO1 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| B2RSH2 | Gnai1 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| O70443 | Gnaz | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| P08752 | Gnai2 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| P20612 | Gnat1 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| P27600 | Gna12 | Guanine nucleotide-binding protein subunit alpha-12 | Mus musculus (Mouse) | SS |
| P27601 | Gna13 | Guanine nucleotide-binding protein subunit alpha-13 | Mus musculus (Mouse) | SS |
| P50149 | Gnat2 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| Q3V3I2 | Gnat3 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| Q9DC51 | Gnai3 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| P21279 | Gnaq | Guanine nucleotide-binding protein G(q) subunit alpha | Mus musculus (Mouse) | PR |
| P30678 | Gna15 | Guanine nucleotide-binding protein subunit alpha-15 | Mus musculus (Mouse) | PR |
| P18872 | Gnao1 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| Q2PKF4 | GNAQ | Guanine nucleotide-binding protein G(q) subunit alpha | Sus scrofa (Pig) | PR |
| P29797 | GNAS | Guanine nucleotide-binding protein G(s) subunit alpha | Sus scrofa (Pig) | PR |
| P93564 | GPA1 | Guanine nucleotide-binding protein alpha-1 subunit | Solanum tuberosum (Potato) | SS |
| P04897 | Gnai2 | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| P08753 | Gnai3 | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| P19627 | Gnaz | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| P29348 | Gnat3 | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| Q63210 | Gna12 | Guanine nucleotide-binding protein subunit alpha-12 | Rattus norvegicus (Rat) | SS |
| Q6Q7Y5 | Gna13 | Guanine nucleotide-binding protein subunit alpha-13 | Rattus norvegicus (Rat) | SS |
| P10824 | Gnai1 | Guanine nucleotide-binding protein G subunit alpha-1 | Rattus norvegicus (Rat) | EV |
| P82471 | Gnaq | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| O88302 | Gna15 | Guanine nucleotide-binding protein subunit alpha-15 | Rattus norvegicus (Rat) | SS |
| Q9JID2 | Gna11 | Guanine nucleotide-binding protein subunit alpha-11 | Rattus norvegicus (Rat) | SS |
| P38406 | Gnal | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| P59215 | Gnao1 | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| Q63803 | Gnas | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| Q0DJ33 | GPA1 | Guanine nucleotide-binding protein alpha-1 subunit | Oryza sativa subsp. japonica (Rice) | SS |
| O76584 | gpa-11 | Guanine nucleotide-binding protein alpha-11 subunit | Caenorhabditis elegans | SS |
| P28051 | gpa-1 | Guanine nucleotide-binding protein alpha-1 subunit | Caenorhabditis elegans | SS |
| P28052 | gpa-3 | Guanine nucleotide-binding protein alpha-3 subunit | Caenorhabditis elegans | SS |
| P51875 | goa-1 | Guanine nucleotide-binding protein G | Caenorhabditis elegans | SS |
| P91907 | gpa-15 | Guanine nucleotide-binding protein alpha-15 subunit | Caenorhabditis elegans | SS |
| Q18434 | odr-3 | Guanine nucleotide-binding protein alpha-17 subunit | Caenorhabditis elegans | SS |
| Q20907 | gpa-8 | Guanine nucleotide-binding protein alpha-8 subunit | Caenorhabditis elegans | PR |
| Q21917 | gpa-7 | Guanine nucleotide-binding protein alpha-7 subunit | Caenorhabditis elegans | SS |
| Q93743 | gpa-6 | Guanine nucleotide-binding protein alpha-6 subunit | Caenorhabditis elegans | SS |
| Q9BIG4 | gpa-10 | Guanine nucleotide-binding protein alpha-10 subunit | Caenorhabditis elegans | PR |
| Q9BIG5 | gpa-4 | Guanine nucleotide-binding protein alpha-4 subunit | Caenorhabditis elegans | SS |
| Q9N2V6 | gpa-16 | Guanine nucleotide-binding protein alpha-16 subunit | Caenorhabditis elegans | SS |
| Q9XTB2 | gpa-13 | Guanine nucleotide-binding protein alpha-13 subunit | Caenorhabditis elegans | SS |
| P49084 | GPA1 | Guanine nucleotide-binding protein alpha-1 subunit | Glycine max (Soybean) (Glycine hispida) | SS |
| O80462 | XLG1 | Extra-large guanine nucleotide-binding protein 1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| P18064 | GPA1 | Guanine nucleotide-binding protein alpha-1 subunit | Arabidopsis thaliana (Mouse-ear cress) | SS |
| P26981 | GPA1 | Guanine nucleotide-binding protein alpha-1 subunit | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAASELYTKF | ARVWIPDPEE | VWKSAELLKD | YKPGDKVLLL | HLEEGKDLEY | RLDPKTGELP |
| 70 | 80 | 90 | 100 | 110 | 120 |
| HLRNPDILVG | ENDLTALSYL | HEPAVLHNLR | VRFIDSKLIY | TYCGIVLVAI | NPYEQLPIYG |
| 130 | 140 | 150 | 160 | 170 | 180 |
| EDIINAYSGQ | NMGDMDPHIF | AVAEEAYKQM | ARDERNQSII | VSGESGAGKT | VSAKYAMRYF |
| 190 | 200 | 210 | 220 | 230 | 240 |
| ATVSGSASEA | NVEEKVLASN | PIMESIGNAK | TTRNDNSSRF | GKYIEIGFDK | RYRIIGANMR |
| 250 | 260 | 270 | 280 | 290 | 300 |
| TYLLEKSRVV | FQAEEERNYH | IFYQLCASAK | LPEFKMLRLG | NADSFHYTKQ | GGSPMIEGVD |
| 310 | 320 | 330 | 340 | 350 | 360 |
| DAKEMAHTRQ | ACTLLGISES | YQMGIFRILA | GILHLGNVGF | ASRDSDSCTI | PPKHEPLTIF |
| 370 | 380 | 390 | 400 | 410 | 420 |
| CDLMGVDYEE | MCHWLCHRKL | ATATETYIKP | ISKLQATNAR | DALAKHIYAK | LFNWIVDHVN |
| 430 | 440 | 450 | 460 | 470 | 480 |
| QALHSAVKQH | SFIGVLDIYG | FETFEINSFE | QFCINYANEK | LQQQFNMHVF | KLEQEEYMKE |
| 490 | 500 | 510 | 520 | 530 | 540 |
| QIPWTLIDFY | DNQPCINLIE | SKLGILDLLD | EECKMPKGTD | DTWAQKLYNT | HLNKCALFEK |
| 550 | 560 | 570 | 580 | 590 | 600 |
| PRMSNKAFII | KHFADKVEYQ | CEGFLEKNKD | TVFEEQIKVL | KSSKFKMLPE | LFQDDEKAIS |
| 610 | 620 | 630 | 640 | 650 | 660 |
| PTSATSSGRT | PLTRVPVKPT | KGRPGQTAKE | HKKTVGHQFR | NSLHLLMETL | NATTPHYVRC |
| 670 | 680 | 690 | 700 | 710 | 720 |
| IKPNDFKFPF | TFDEKRAVQQ | LRACGVLETI | RISAAGFPSR | WTYQEFFSRY | RVLMKQKDVL |
| 730 | 740 | 750 | 760 | 770 | 780 |
| GDRKQTCKNV | LEKLILDKDK | YQFGKTKIFF | RAGQVAYLEK | LRADKLRAAC | IRIQKTIRGW |
| 790 | 800 | 810 | 820 | 830 | 840 |
| LLRKRYLCMQ | RAAITVQRYV | RGYQARCYAK | FLRRTKAATT | IQKYWRMYVV | RRRYKIRRAA |
| 850 | 860 | 870 | 880 | 890 | 900 |
| TIVIQSYLRG | YLTRNRYRKI | LREYKAVIIQ | KRVRGWLART | HYKRTMKAIV | YLQCCFRRMM |
| 910 | 920 | 930 | 940 | 950 | 960 |
| AKRELKKLKI | EARSVERYKK | LHIGMENKIM | QLQRKVDEQN | KDYKCLMEKL | TNLEGVYNSE |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| TEKLRNDVER | LQLSEEEAKV | ATGRVLSLQE | EIAKLRKDLE | QTRSEKKSIE | ERADKYKQET |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| DQLVSNLKEE | NTLLKQEKET | LNHRIVEQAK | EMTETMERKL | VEETKQLELD | LNDERLRYQN |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| LLNEFSRLEE | RYDDLKEEMT | LMLNVPKPGH | KRTDSTHSSN | ESEYTFSSEF | AETEDIAPRT |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| EEPIEKKVPL | DMSLFLKLQK | RVTELEQEKQ | LMQDELDRKE | EQVFRSKAKE | EERPQIRGAE |
| 1210 | 1220 | 1230 | 1240 | 1250 | 1260 |
| LEYESLKRQE | LESENKKLKN | ELNELRKALS | EKSAPEVTAP | GAPAYRVLME | QLTSVSEELD |
| 1270 | 1280 | 1290 | 1300 | 1310 | 1320 |
| VRKEEVLILR | SQLVSQKEAI | QPKDDKNTMT | DSTILLEDVQ | KMKDKGEIAQ | AYIGLKETNR |
| 1330 | 1340 | 1350 | 1360 | 1370 | 1380 |
| LLESQLQSQK | RSHENEAEAL | RGEIQSLKEE | NNRQQQLLAQ | NLQLPPEARI | EASLQHEITR |
| 1390 | 1400 | 1410 | 1420 | 1430 | 1440 |
| LTNENLYFEE | LYADDPKKYQ | SYRISLYKRM | IDLMEQLEKQ | DKTVRKLKKQ | LKVFAKKIGE |
| 1450 | 1460 | 1470 | 1480 | 1490 | 1500 |
| LEVGQMENIS | PGQIIDEPIR | PVNIPRKEKD | FQGMLEYKRE | DEQKLVKNLI | LELKPRGVAV |
| 1510 | 1520 | 1530 | 1540 | 1550 | 1560 |
| NLIPGLPAYI | LFMCVRHADY | LNDDQKVRSL | LTSTINSIKK | VLKKRGDDFE | TVSFWLSNTC |
| 1570 | 1580 | 1590 | 1600 | 1610 | 1620 |
| RFLHCLKQYS | GEEGFMKHNT | SRQNEHCLTN | FDLAEYRQVL | SDLAIQIYQQ | LVRVLENILQ |
| 1630 | 1640 | 1650 | 1660 | 1670 | 1680 |
| PMIVSGMLEH | ETIQGVSGVK | PTGLRKRTSS | IADEGTYTLD | SILRQLNSFH | SVMCQHGMDP |
| 1690 | 1700 | 1710 | 1720 | 1730 | 1740 |
| ELIKQVVKQM | FYIVGAITLN | NLLLRKDMCS | WSKGMQIRYN | VSQLEEWLRD | KNLMNSGAKE |
| 1750 | 1760 | 1770 | 1780 | 1790 | 1800 |
| TLEPLIQAAQ | LLQVKKKTDD | DAEAICSMCN | ALTTAQIVKV | LNLYTPVNEF | EERVSVSFIR |
| 1810 | 1820 | 1830 | 1840 | 1850 | |
| TIQMRLRDRK | DSPQLLMDAK | HIFPVTFPFN | PSSLALETIQ | IPASLGLGFI | ARV |