AiPD: Autoinhibited Protein Database

P62205

Gene name	plk1
Protein name	Serine/threonine-protein kinase PLK1
Names	EC 2.7.11.21 , Plx1 , Polo-like kinase 1 , PLK-1
Species	Mus musculus (Mouse)
KEGG Pathway	xtr:407937
EC number
Protein Class

Descriptions

Microrchidia 3 is a human protein linked to autoimmune disorders, Down syndrome, and cancer. The extensive ATPase:CW interface stabilizes the protein fold and inhibits the catalytic activity of MORC3. The CW domain sterically impedes binding of the ATPase domain to DNA. MORC3 autoinhibition is released by disrupting the intramolecular ATPase:DW coupling through the competitive interaction of CW with histone H3 tail. Binding of CW to H3 leads to a marked rearrangement in the ATPase-CW cassette, which frees the DNA-binding site in active MORC3.

Autoinhibitory domains (AIDs)

361-598 (Polo box domain) SS

Target domain	44-296 (Protein kinase domain)
Relief mechanism	PTM
Assay

AID

361-598 (Polo box domain)

Target domain

44-296 (Protein kinase domain)

Relief mechanism

PTM (Phosphorylation at Thr-210 by the upstream kinase)

Assay

Accessory elements

184-207 (Activation loop from InterPro)

Target domain	36-300 (Protein kinase domain)
Relief mechanism
Assay

References

Cheng KY et al. (2003) "The crystal structure of the human polo-like kinase-1 polo box domain and its phospho-peptide complex", The EMBO journal, 22, 5757-68

Zhu K et al. (2016) "Phospho-Pon Binding-Mediated Fine-Tuning of Plk1 Activity", Structure (London, England : 1993), 24, 1110-9

Jang YJ et al. (2002) "Functional studies on the role of the C-terminal domain of mammalian polo-like kinase", Proceedings of the National Academy of Sciences of the United States of America, 99, 1984-9

Autoinhibited structure

Activated structure

1 structures for P62205

Entry ID	Method	Resolution	Chain	Position	Source
AF-P62205-F1	Predicted				AlphaFoldDB

No variants for P62205

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
No variants for P62205

No associated diseases with P62205

2 regional properties for P62205

Type	Name	Position	InterPro Accession
domain	Zinc finger, CW-type	404 - 454	IPR011124
domain	Morc, S5 domain 2-like	249 - 382	IPR041006

Functions

		Description
EC Number
Subcellular Localization	Nucleus, nucleoplasm Nucleus matrix Nucleus, PML body Chromosome	Also found in PML-independent nuclear bodies Localization to nuclear bodies is ATP-dependent
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

6 GO annotations of cellular component

Name	Definition
centrosome	A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
kinetochore	A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
midbody	A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
spindle pole	Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.

3 GO annotations of molecular function

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
protein serine kinase activity	Catalysis of the reactions
protein serine/threonine kinase activity	Catalysis of the reactions

7 GO annotations of biological process

Name	Definition
cell division	The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
mitotic cell cycle	Progression through the phases of the mitotic cell cycle, the most common eukaryotic cell cycle, which canonically comprises four successive phases called G1, S, G2, and M and includes replication of the genome and the subsequent segregation of chromosomes into daughter cells. In some variant cell cycles nuclear replication or nuclear division may not be followed by cell division, or G1 and G2 phases may be absent.
mitotic G2 DNA damage checkpoint signaling	A mitotic cell cycle checkpoint that detects and negatively regulates progression through the G2/M transition of the cell cycle in response to DNA damage.
mitotic spindle organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.
negative regulation of double-strand break repair via homologous recombination	Any process that stops, prevents, or reduces the frequency, rate or extent of double-strand break repair via homologous recombination.
protein localization to chromatin	Any process in which a protein is transported to, or maintained at, a part of a chromosome that is organized into chromatin.
protein phosphorylation	The process of introducing a phosphate group on to a protein.

9 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q2TA25	PLK1	Serine/threonine-protein kinase PLK1	Bos taurus (Bovine)	SS
O97143	SAK	Serine/threonine-protein kinase PLK4	Drosophila melanogaster (Fruit fly)	EV
P52304	polo	Serine/threonine-protein kinase polo	Drosophila melanogaster (Fruit fly)	SS
P53350	PLK1	Serine/threonine-protein kinase PLK1	Homo sapiens (Human)	EV
Q07832	Plk1	Serine/threonine-protein kinase PLK1	Mus musculus (Mouse)	EV
Q62673	Plk1	Serine/threonine-protein kinase PLK1	Rattus norvegicus (Rat)	SS
Q20845	plk-3	Serine/threonine-protein kinase plk-3	Caenorhabditis elegans	PR
Q9N2L7	plk-2	Serine/threonine-protein kinase plk-2	Caenorhabditis elegans	SS
P34331	plk-1	Serine/threonine-protein kinase plk-1	Caenorhabditis elegans	SS

10	20	30	40	50	60
MAAQPPTGIR	LSALCPKFLH	TNSTSHTWPF	SAVAELIDNA	YDPDVNAKQI	WIDKTVISDH
70	80	90	100	110	120
ICLTFTDNGN	GMTADKLHKM	LSFGFSDKVT	MNGHVPVGLY	GNGFKSGSMR	LGKDAMVFTK
130	140	150	160	170	180
NGETMSVGFL	SQTYLEVIKA	EHVVVPIVTF	NKHRQMINLT	ESKASLAAIL	EHSLFSTEQK
190	200	210	220	230	240
LLAELNAIMG	KKGTRIIIWN	LRSYKNATEF	DFEKDKYDIR	IPEDLDETAG	RKGYKKQERM
250	260	270	280	290	300
DQIAPESDYS	LRAYCSILYL	KPRMQIIIRG	QKVKTQLVSK	SLAYIERDVY	RPKFLTRTVR
310	320	330	340	350	360
ITFGFNCRNK	DHYGIMMYHK	NRLIKAYEKV	GCQLKANNMG	VGVVGIIECN	FLKPTHNKQD
370	380	390	400	410	420
FDYTNEYRLT	ILALGEKLND	YWNEMKVKKN	AEYPVNLPVE	DIQKRPDQTW	VQCDACLKWR
430	440	450	460	470	480
KLPDGIDQLP	EKWYCSNNPD	PQFRNCEVPE	EPEDEDLVHP	TYEKTYKKTS	KERFRIRQPE
490	500	510	520	530	540
ILPRILPQIN	PELLYQTSVS	SQSFSPVKES	VPRPHLSEVT	SPFAARIINL	NLASPASEPE
550	560	570	580	590	600
NSSMKRKLGV	HSSILNAKTR	RLSNPPVENS	SYKNDDDEDV	IILEENSTPK	PAVDLEVKSD
610	620	630	640	650	660
IEVKSEQSHT	EQSGIHVDLV	SSPKPCVQAS	STSTSTSRSD	PGITVSTQTD	APGLTVKKEE
670	680	690	700	710	720
SMEEDMGVRN	GTATLSCVGT	EAKVQETSAE	SVDATSHQLQ	ELRSELLVVT	QERDDYKRQC
730	740	750	760	770	780
QMFTDQIQVL	QQRLLEMNDK	CVKKEKCHQS	TETDAVFLLD	SVNGQAESLD	HLGSQYQQAL
790	800	810	820	830	840
QEIERLKRQC	SALQQVKSEC	SQASCTESKS	EVDEMAVQLD	DVFRQLDKCT	IERDQYKNEV
850	860	870	880	890	900
QLLEIEKSHI	HSQCEELQTE	VEQLKSTGQQ	AAADGSTASN	AEEPVSYVDG	ESLKLRSLRV
910	920	930	940
NVGQLLAMIV	PDLDLQQVNY	DVDVVDEILG	QVVEQMSEIS	ST