P54358
PR
Gene name |
PolD1 |
Protein name |
DNA polymerase delta catalytic subunit |
Names |
3'-5' exodeoxyribonuclease |
Species |
Drosophila melanogaster (Fruit fly) |
KEGG Pathway |
dme:Dmel_CG5949 |
EC number |
2.7.7.7: Nucleotidyltransferases |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for P54358
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P54358-F1 | Predicted | AlphaFoldDB |
No variants for P54358
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P54358 | |||||
No associated diseases with P54358
12 regional properties for P54358
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | C2 domain | 158 - 277 | IPR000008 |
| domain | Protein kinase domain | 339 - 597 | IPR000719 |
| domain | AGC-kinase, C-terminal | 598 - 668 | IPR000961 |
| domain | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain | 36 - 87 | IPR002219-1 |
| domain | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain | 101 - 153 | IPR002219-2 |
| active_site | Serine/threonine-protein kinase, active site | 459 - 471 | IPR008271 |
| binding_site | Protein kinase, ATP binding site | 345 - 368 | IPR017441 |
| domain | Diacylglycerol/phorbol-ester binding | 34 - 48 | IPR020454-1 |
| domain | Diacylglycerol/phorbol-ester binding | 50 - 59 | IPR020454-2 |
| domain | Diacylglycerol/phorbol-ester binding | 63 - 74 | IPR020454-3 |
| domain | Diacylglycerol/phorbol-ester binding | 140 - 152 | IPR020454-4 |
| domain | Classical Protein Kinase C alpha, catalytic domain | 328 - 668 | IPR034663 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.7.7 | Nucleotidyltransferases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| delta DNA polymerase complex | A multimeric DNA polymerase enzyme complex which differs in composition amongst species; in humans it is a heterotetramer of four subunits of approximately 125, 50, 68 and 12kDa, while in S. cerevisiae, it has three different subunits which form a heterotrimer, and the active enzyme is a dimer of this heterotrimer. Functions in DNA replication, mismatch repair and excision repair. |
| nucleoplasm | That part of the nuclear content other than the chromosomes or the nucleolus. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| 3'-5'-exodeoxyribonuclease activity | Catalysis of the sequential cleavage of mononucleotides from a free 3' terminus of a DNA molecule. |
| 4 iron, 4 sulfur cluster binding | Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. |
| DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). |
| DNA-directed DNA polymerase activity | Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1); the synthesis of DNA from deoxyribonucleotide triphosphates in the presence of a DNA template and a 3'hydroxyl group. |
| metal ion binding | Binding to a metal ion. |
| nucleotide binding | Binding to a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose. |
4 GO annotations of biological process
| Name | Definition |
|---|---|
| base-excision repair, gap-filling | Repair of the damaged strand by the combined action of an apurinic endouclease that degrades a few bases on the damaged strand and a polymerase that synthesizes a 'patch' in the 5' to 3' direction, using the undamaged strand as a template. |
| DNA replication proofreading | Correction of replication errors by DNA polymerase using a 3'-5' exonuclease activity. |
| DNA-templated DNA replication | A DNA replication process that uses parental DNA as a template for the DNA-dependent DNA polymerases that synthesize the new strands. |
| nucleotide-excision repair, DNA gap filling | Repair of the gap in the DNA helix by DNA polymerase and DNA ligase after the portion of the strand containing the lesion has been removed by pyrimidine-dimer repair enzymes. |
5 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P28339 | POLD1 | DNA polymerase delta catalytic subunit | Bos taurus (Bovine) | PR |
| P28340 | POLD1 | DNA polymerase delta catalytic subunit | Homo sapiens (Human) | PR |
| P52431 | Pold1 | DNA polymerase delta catalytic subunit | Mus musculus (Mouse) | PR |
| O54747 | Pold1 | DNA polymerase delta catalytic subunit | Rattus norvegicus (Rat) | PR |
| Q9LRE6 | POLD1 | DNA polymerase delta catalytic subunit | Oryza sativa subsp japonica (Rice) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MDGKRKFNGT | SNGHAKKPRN | PDDDEEMGFE | AELAAFENSE | DMDQTLLMGD | GPENQTTSER |
| 70 | 80 | 90 | 100 | 110 | 120 |
| WSRPPPPELD | PSKHNLEFQQ | LDVENYLGQP | LPGMPGAQIG | PVPVVRMFGV | TMEGNSVCCH |
| 130 | 140 | 150 | 160 | 170 | 180 |
| VHGFCPYFYI | EAPSQFEEHH | CEKLQKALDQ | KVIADIRNNK | DNVQEAVLMV | ELVEKLNIHG |
| 190 | 200 | 210 | 220 | 230 | 240 |
| YNGDKKQRYI | KISVTLPRFV | AAASRLLKKE | VIMSEIDFQD | CRAFENNIDF | DIRFMVDTDV |
| 250 | 260 | 270 | 280 | 290 | 300 |
| VGCNWIELPM | GHWRIRNSHS | KPLPESRCQI | EVDVAFDRFI | SHEPEGEWSK | VAPFRILSFD |
| 310 | 320 | 330 | 340 | 350 | 360 |
| IECAGRKGIF | PEAKIDPVIQ | IANMVIRQGE | REPFIRNVFT | LNECAPIIGS | QVLCHDKETQ |
| 370 | 380 | 390 | 400 | 410 | 420 |
| MLDKWSAFVR | EVDPDILTGY | NINNFDFPYL | LNRAAHLKVR | NFEYLGRIKN | IRSVIKEQML |
| 430 | 440 | 450 | 460 | 470 | 480 |
| QSKQMGRREN | QYVNFEGRVP | FDLLFVLLRD | YKLRSYTLNA | VSYHFLQEQK | EDVHHSIITD |
| 490 | 500 | 510 | 520 | 530 | 540 |
| LQNGDEQTRR | RLAMYCLKDA | YLPLRLLEKL | MAIVNYMEMA | RVTGVPLESL | LTRGQQIKVL |
| 550 | 560 | 570 | 580 | 590 | 600 |
| SQLLRKAKTK | GFIMPSYTSQ | GSDEQYEGAT | VIEPKRGYYA | DPISTLDFAS | LYPSIMMAHN |
| 610 | 620 | 630 | 640 | 650 | 660 |
| LCYTTLVLGG | TREKLRQQEN | LQDDQVERTP | ANNYFVKSEV | RRGLLPEILE | SLLAARKRAK |
| 670 | 680 | 690 | 700 | 710 | 720 |
| NDLKVETDPF | KRKVLDGRQL | ALKISANSVY | GFTGAQVGKL | PCLEISGSVT | AYGRTMIEMT |
| 730 | 740 | 750 | 760 | 770 | 780 |
| KNEVESHYTQ | ANGYENNAVV | IYGDTDSVMV | NFGVKTLERS | MELGREAAEL | VSSKFVHPIK |
| 790 | 800 | 810 | 820 | 830 | 840 |
| LEFEKVYYPY | LLINKKRYAG | LYFTRPDTYD | KMDCKGIETV | RRDNSPLVAN | LMNSCLQKLL |
| 850 | 860 | 870 | 880 | 890 | 900 |
| IERDPDGAVA | YVKQVIADLL | CNRIDISHLV | ITKELAKTDY | AAKQAHVELA | AKMKKRDPGT |
| 910 | 920 | 930 | 940 | 950 | 960 |
| APKLGDRVPY | VICAAAKNTP | AYQKAEDPLY | VLENSVPIDA | TYYLEQQLSK | PLLRIFEPIL |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| GDNAESILLK | GEHTRTRTVV | TSKVGGLAGF | MTKKTSCLGC | KSLMPKGYEQ | ACLCPHCEPR |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| MSELYQKEVG | AKRELEETFS | RLWTECQRCQ | ESLHEEVICS | NRDCPIFYMR | QKVRMDLDNQ |
| 1090 | |||||
| EKRVLRFGLA | EW |