P41515

PR
Gene name
TOP2A (TOP-2, TOP2)
Protein name
DNA topoisomerase 2-alpha
Names
DNA topoisomerase II, alpha isozyme
Species
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
KEGG Pathway
cge:100689304
EC number
5.6.2.2: Enzymes altering nucleic acid conformation
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-54 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P41515

Entry ID Method Resolution Chain Position Source
AF-P41515-F1 Predicted AlphaFoldDB

1 variants for P41515

Variant ID(s) Position Change Description Diseaes Association Provenance
493 R>Q cells resistant to the antineoplastic agents VP-16 and VM-26 [UniProt] No

No associated diseases with P41515

8 regional properties for P41515

Type Name Position InterPro Accession
domain DNA topoisomerase, type IIA, domain A 692 - 1169 IPR002205
domain Histidine kinase/HSP90-like ATPase 78 - 223 IPR003594
domain TOPRIM domain 454 - 571 IPR006171
domain DTHCT 1430 - 1517 IPR012542
domain DNA topoisomerase, type IIA, subunit B, domain 2 265 - 425 IPR013506
conserved_site DNA topoisomerase, type IIA, conserved site 458 - 466 IPR018522
domain C-terminal associated domain of TOPRIM 572 - 710 IPR031660
domain DNA topoisomerase 2, TOPRIM domain 454 - 574 IPR034157

Functions

Description
EC Number 5.6.2.2 Enzymes altering nucleic acid conformation
Subcellular Localization
  • Cytoplasm
  • Nucleus, nucleoplasm
  • Nucleus
  • Nucleus, nucleolus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

11 GO annotations of cellular component

Name Definition
centriole A cellular organelle, found close to the nucleus in many eukaryotic cells, consisting of a small cylinder with microtubular walls, 300-500 nm long and 150-250 nm in diameter. It contains nine short, parallel, peripheral microtubular fibrils, each fibril consisting of one complete microtubule fused to two incomplete microtubules. Cells usually have two centrioles, lying at right angles to each other. At division, each pair of centrioles generates another pair and the twin pairs form the pole of the mitotic spindle.
chromosome, centromeric region The region of a chromosome that includes the centromeric DNA and associated proteins. In monocentric chromosomes, this region corresponds to a single area of the chromosome, whereas in holocentric chromosomes, it is evenly distributed along the chromosome.
condensed chromosome A highly compacted molecule of DNA and associated proteins resulting in a cytologically distinct structure.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex Complex that possesses DNA topoisomerase II (double strand cut, ATP-hydrolyzing) activity.
male germ cell nucleus The nucleus of a male germ cell, a reproductive cell in males.
nuclear chromosome A chromosome that encodes the nuclear genome and is found in the nucleus of a eukaryotic cell during the cell cycle phases when the nucleus is intact.
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
ribonucleoprotein complex A macromolecular complex that contains both RNA and protein molecules.

11 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
DNA binding, bending The activity of binding selectively and non-covalently to and distorting the original structure of DNA, typically a straight helix, into a bend, or increasing the bend if the original structure was intrinsically bent due to its sequence.
DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Catalysis of a DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined, driven by ATP hydrolysis. The enzyme changes the linking number in multiples of 2.
histone deacetylase binding Binding to histone deacetylase.
magnesium ion binding Binding to a magnesium (Mg) ion.
protein C-terminus binding Binding to a protein C-terminus, the end of a peptide chain at which the 1-carboxyl function of a constituent amino acid is not attached in peptide linkage to another amino-acid residue.
protein heterodimerization activity Binding to a nonidentical protein to form a heterodimer.
protein homodimerization activity Binding to an identical protein to form a homodimer.
protein kinase C binding Binding to protein kinase C.
ubiquitin binding Binding to ubiquitin, a protein that when covalently bound to other cellular proteins marks them for proteolytic degradation.

14 GO annotations of biological process

Name Definition
apoptotic chromosome condensation The compaction of chromatin during apoptosis.
cellular response to DNA damage stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
chromosome segregation The process in which genetic material, in the form of chromosomes, is organized into specific structures and then physically separated and apportioned to two or more sets. In eukaryotes, chromosome segregation begins with the condensation of chromosomes, includes chromosome separation, and ends when chromosomes have completed movement to the spindle poles.
DNA ligation The re-formation of a broken phosphodiester bond in the DNA backbone, carried out by DNA ligase.
DNA topological change The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number.
embryonic cleavage The first few specialized divisions of an activated animal egg.
female meiotic nuclear division A cell cycle process by which the cell nucleus divides as part of a meiotic cell cycle in the female germline.
hematopoietic progenitor cell differentiation The process in which precursor cell type acquires the specialized features of a hematopoietic progenitor cell, a class of cell types including myeloid progenitor cells and lymphoid progenitor cells.
negative regulation of DNA duplex unwinding Any process that stops, prevents or reduces the frequency, rate or extent of DNA duplex unwinding.
positive regulation of apoptotic process Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process.
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate Any process that activates or increases the frequency, rate or extent of retroviral genome replication.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
regulation of circadian rhythm Any process that modulates the frequency, rate or extent of a circadian rhythm. A circadian rhythm is a biological process in an organism that recurs with a regularity of approximately 24 hours.
rhythmic process Any process pertinent to the generation and maintenance of rhythms in the physiology of an organism.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MELSPLQPVN ENMQMNKKKN EDAKKRLSIE RIYQKKTQLE HILLRPDTYI GSVELVTQQM
70 80 90 100 110 120
WVYDEDVGIN YREVTFVPGL YKIFDEILVN AADNKQRDPK MSCIRVTIDP ENNLISIWNN
130 140 150 160 170 180
GKGIPVVEHK VEKMYVPALI FGQLLTSSNY DDDEKKVTGG RNGYGAKLCN IFSTRFTVET
190 200 210 220 230 240
ASKEYKKMFK QTWMDNMGRA GDMELKPFNG EDYTCITFQP DLSKFKMQSL DKDIVALMVR
250 260 270 280 290 300
RAYDIAGSTK DVKVFLNGNK LPVKGFRSYV DMYLKDKLDE TGNALKVVHE QVNPRWEVCL
310 320 330 340 350 360
TMSEKGFQQI SFVNSIATSK GGRHVDYVAD QIVSKLVDVV KKKNKGGVAV KAHQVKNHMW
370 380 390 400 410 420
IFVNALIENP TFDSQTKENM TLQAKSFGST CQLSEKFIKA AIGCGIVESI LNWVKFKAQI
430 440 450 460 470 480
QLNKKCSAVK HNRIKGIPKL DDANDAGSRN STECTLILTE GDSAKTLAVS GLGVVGRDKY
490 500 510 520 530 540
GVFPLRGKIL NVREASHKQI MENAEINNII KIVGLQYKKN YEDEDSLKTL RYGKIMIMTD
550 560 570 580 590 600
QDQDGSHIKG LLINFIHHNW PSLLRHRFLE EFITPIVKVS KNKQELAFYS LPEFEEWKSS
610 620 630 640 650 660
TPNHKKWKVK YYKGLGTSTS KEAKEYFADM KRHRIQFKYS GPEDDAAISL AFSKKQVDDR
670 680 690 700 710 720
KEWLTHFMED RRQRKLLGLP EDYLYGQTTT YLTYNDFINK ELILFSNSDN ERSIPSMVDG
730 740 750 760 770 780
LKPGQRKVLF TCFKRNDKRE VKVAQLAGSV AEMSSYHHGE MSLMMTIINL AQNFVGSNNL
790 800 810 820 830 840
NLLQPIGQFG TRLHGGKDSA SPRYIFTMLS PLTRLLFPPK DDHTLKFLYD DNQRVEPEWY
850 860 870 880 890 900
IPIIPMVLIN GAEGIGTGWS CKIPNFDIRE VVNNIRRLLD GEEPLPMLPS YKNFKGTIEE
910 920 930 940 950 960
LASNQYVING EVAILNSTTI EISELPIRTW TQTYKEQVLE PMLNGTEKTP PLITDYREYH
970 980 990 1000 1010 1020
TDTTVKFVIK MTEEKLAEAE RVGLHKVFKL QTSLTCNSMV LFDHVGCLKK YDTVLDILKD
1030 1040 1050 1060 1070 1080
FFELRLKYYG LRKEWLLGML GAESAKLNNQ ARFILEKIDG KIIIENKPKK ELIKVLIQRG
1090 1100 1110 1120 1130 1140
YDSDPVKAWK EAQQKVPDEE ENEESDNENS DSVAESGPTF NYLLDMPLWY LTKEKKDELC
1150 1160 1170 1180 1190 1200
KQRNEKEQEL NTLKNKSPSD LWKEDLAVFI EELEVVEAKE KQDEQVGLPG KGGKAKGKKA
1210 1220 1230 1240 1250 1260
QMSEVLPSPH GKRVIPQVTM EMKAEAEKKI RKKIKSENVE GTPTENGLEL GSLKQRIEKK
1270 1280 1290 1300 1310 1320
QKKEPGAMTK KQTTLAFKPI KKGKKRNPWS DSESDMSSNE SNVDVPPREK DPRRAATKAK
1330 1340 1350 1360 1370 1380
FTMDLDSDED FSGSDGKDED EDFFPLDTTP PKTKIPQKNT KKALKPQKSA MSGDPESDEK
1390 1400 1410 1420 1430 1440
DSVPASPGPP AADLPADTEQ LKPSSKQTVA VKKTATKSQS STSTAGTKKR AVPKGSKSDS
1450 1460 1470 1480 1490 1500
ALNAHGPEKP VPAKAKNSRK RKQSSSDDSD SDFEKVVSKV AASKKSKGEN QDFRVDLDET
1510 1520
MVPRAKSGRA KKPIKYLEES DDDDLF