P37229

SS
Gene name
Protein name
Malate dehydrogenase [NADP] 2, chloroplastic
Names
NADP-MDH-2
Species
Sorghum bicolor (Sorghum) (Sorghum vulgare)
KEGG Pathway
EC number
1.1.1.82: With NAD(+) or NADP(+) as acceptor
Protein Class

Descriptions

The chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH) catalyzes the reduction of oxaloacetate into L-malate. The enzyme exhibits extensions at both the N- and C-termini. The N-terminal disulfide motif is inserted in a cleft between the two subunits of the dimer, thereby locking the domains in each subunit. The C-terminal extension (C-terminal inhibitory peptide) also contains a regulatory disulfide bridge. The C-terminal disulfide keeps the C-terminal residues tight to the enzyme surface and blocks access to the active site. Reduction of the N-terminal disulfide may release the stopper between the domains and give the enzyme the flexibility. Simultaneous reduction of the C-terminal disulfide may free the C-terminal residues from binding to the enzyme and make the active site accessible.

Autoinhibitory domains (AIDs)

57-75 (N-terminal disulfide motif) SS

Target domain

91-411 (MDH domain)

Relief mechanism

Others

Assay

408-432 (C-terminal disulfide motif) SS

Target domain

91-411 (MDH domain)

Relief mechanism

Others

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P37229

Entry ID Method Resolution Chain Position Source
AF-P37229-F1 Predicted AlphaFoldDB

No variants for P37229

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P37229

No associated diseases with P37229

1 regional properties for P37229

Type Name Position InterPro Accession
domain Lactate/malate dehydrogenase, N-terminal 91 - 238 IPR001236

Functions

Description
EC Number 1.1.1.82 With NAD(+) or NADP(+) as acceptor
Subcellular Localization
  • Plastid, chloroplast
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
chloroplast A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

1 GO annotations of molecular function

Name Definition
malate dehydrogenase (NADP+) activity Catalysis of the reaction: (S)-malate + NADP+ = oxaloacetate + NADPH + H+.

2 GO annotations of biological process

Name Definition
carbohydrate metabolic process The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y.
malate metabolic process The chemical reactions and pathways involving malate, the anion of hydroxybutanedioic acid, a chiral hydroxydicarboxylic acid. The (+) enantiomer is an important intermediate in metabolism as a component of both the TCA cycle and the glyoxylate cycle.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P15719 Malate dehydrogenase [NADP], chloroplastic Zea mays (Maize) SS
P17606 Malate dehydrogenase [NADP] 1, chloroplastic Sorghum bicolor (Sorghum) (Sorghum vulgare) EV
10 20 30 40 50 60
MGLSTAYSPA GSGLVPAPLA RAARRRSVQV RRPRLATVRC SVVDAAKQVQ DGVATAVGGG
70 80 90 100 110 120
AASGNECFGV FCNIYDLKAE DKTKSWKKLV TIAVSGAAGM ISNHLLFKLA SGEVFGQDQP
130 140 150 160 170 180
IALKLLGSER SFQALEGVRM ELEDSLYPLL REVSIGIGPY EVFQDVDWAL LIGAKPRGPG
190 200 210 220 230 240
MERAALLDIN GQIFADQGKA LNAVASRNVK VLVVGNPCNT NALICLKNTP NIPAKNFHAL
250 260 270 280 290 300
TRLDENRAKC QIALKAGVFY DKVSNVTIWG NHSTTQVPDF LNAKIDGRPV KEIIQDTKWL
310 320 330 340 350 360
EEEFTMTVQK RGGVLIQKWG RSSAASTAVS IVDAIKSLVT PTPEGEWFST GVYTTGNPYG
370 380 390 400 410 420
IAEDIVFSMP CRSKGDGDYE LATDVSMDDF LWERIKKSEA ELLAEKKCVA HLTGEGDAFC
430
DLPEDTMLPG EN