P26659

PR
Gene name
rad15
Protein name
General transcription and DNA repair factor IIH helicase subunit XPD
Names
TFIIH subunit XPD, DNA repair helicase RAD3 homolog, Protein rhp3, DNA repair helicase rad15, RNA polymerase II transcription factor B subunit rad15, TFB subunit rad15
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
KEGG Pathway
spo:SPAC1D4.12
EC number
3.6.4.12: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

712-772 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P26659

Entry ID Method Resolution Chain Position Source
AF-P26659-F1 Predicted AlphaFoldDB

2 variants for P26659

Variant ID(s) Position Change Description Diseaes Association Provenance
I_665455_G_A 225 S>N No Jeffares_SNPs
I_666248_C_G 489 F>L No Jeffares_SNPs

No associated diseases with P26659

5 regional properties for P26659

Type Name Position InterPro Accession
domain Helicase-like, DEXD box c2 type 8 - 279 IPR006554
domain ATP-dependent helicase, C-terminal 523 - 698 IPR006555
domain RAD3-like helicase, DEAD 71 - 255 IPR010614
domain Helical and beta-bridge domain 269 - 411 IPR010643
domain Helicase superfamily 1/2, ATP-binding domain, DinG/Rad3-type 7 - 283 IPR014013

Functions

Description
EC Number 3.6.4.12 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nucleotide-excision repair factor 3 complex One of several protein complexes involved in nucleotide-excision repair; possesses endodeoxynuclease and DNA helicase activities. In S. cerevisiae, it is composed of Rad2p and the core TFIIH-Ssl2p complex (core TFIIH is composed of Rad3p, Tfb1p, Tfb2p, Ssl1p, Tfb4p and Tfb5p. Note that Ssl2p is also called Rad25p).
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
transcription factor TFIIH core complex The 7 subunit core of TFIIH that is a part of either the general transcription factor holo-TFIIH or the nucleotide-excision repair factor 3 complex. In S. cerevisiae/humans the complex is composed of: Ssl2/XPB, Tfb1/p62, Tfb2/p52, Ssl1/p44, Tfb4/p34, Tfb5/p8 and Rad3/XPD.
transcription factor TFIIH holo complex A complex that is capable of kinase activity directed towards the C-terminal Domain (CTD) of the largest subunit of RNA polymerase II and is essential for initiation at RNA polymerase II promoters in vitro. It is composed of the core TFIIH complex and the TFIIK complex.

8 GO annotations of molecular function

Name Definition
4 iron, 4 sulfur cluster binding Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
5'-3' DNA helicase activity Unwinding a DNA helix in the 5' to 3' direction, driven by ATP hydrolysis.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
damaged DNA binding Binding to damaged DNA.
DNA helicase activity Unwinding of a DNA helix, driven by ATP hydrolysis.
metal ion binding Binding to a metal ion.
RNA polymerase II general transcription initiation factor activity A general transcription initiation factor activity that contributes to transcription start site selection and transcription initiation of genes transcribed by RNA polymerase II. The general transcription factors for RNA polymerase II include TFIIB, TFIID, TFIIE, TFIIF, TFIIH and TATA-binding protein (TBP). In most species, RNA polymerase II transcribes all messenger RNAs (mRNAs), most untranslated regulatory RNAs, the majority of the snoRNAs, four of the five snRNAs (U1, U2, U4, and U5), and other small noncoding RNAs. For some small RNAs there is variability between species as to whether it is transcribed by RNA polymerase II or RNA polymerase III. However there are also rare exceptions, such as Trypanosoma brucei, where RNA polymerase I transcribes certain mRNAs in addition to its normal role in rRNA transcription.

6 GO annotations of biological process

Name Definition
nucleotide-excision repair A DNA repair process in which a small region of the strand surrounding the damage is removed from the DNA helix as an oligonucleotide. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. Nucleotide excision repair recognizes a wide range of substrates, including damage caused by UV irradiation (pyrimidine dimers and 6-4 photoproducts) and chemicals (intrastrand cross-links and bulky adducts).
nucleotide-excision repair, DNA duplex unwinding The unwinding, or local denaturation, of the DNA duplex to create a bubble around the site of the DNA damage.
nucleotide-excision repair, DNA incision A process that results in the endonucleolytic cleavage of the damaged strand of DNA. The incision occurs at the junction of single-stranded DNA and double-stranded DNA that is formed when the DNA duplex is unwound.
positive regulation of mitotic recombination Any process that activates or increases the frequency, rate or extent of DNA recombination during mitosis.
transcription by RNA polymerase II The synthesis of RNA from a DNA template by RNA polymerase II (RNAP II), originating at an RNA polymerase II promoter. Includes transcription of messenger RNA (mRNA) and certain small nuclear RNAs (snRNAs).
transcription initiation at RNA polymerase II promoter A transcription initiation process that takes place at a RNA polymerase II gene promoter. Messenger RNAs (mRNA) genes, as well as some non-coding RNAs, are transcribed by RNA polymerase II.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MKFYIDDLPI LFPYPRIYPE QYQYMCDLKH SLDAGGIALL EMPSGTGKTI SLLSLIVSYQ
70 80 90 100 110 120
QHYPEHRKLI YCSRTMSEID KALAELKRLM AYRTSQLGYE EPFLGLGLTS RKNLCLHPSV
130 140 150 160 170 180
RREKNGNVVD ARCRSLTAGF VREQRLAGMD VPTCEFHDNL EDLEPHSLIS NGVWTLDDIT
190 200 210 220 230 240
EYGEKTTRCP YFTVRRMLPF CNVIIYSYHY LLDPKIAERV SRELSKDCIV VFDEAHNIDN
250 260 270 280 290 300
VCIESLSIDL TESSLRKASK SILSLEQKVN EVKQSDSKKL QDEYQKLVRG LQDANAANDE
310 320 330 340 350 360
DQFMANPVLP EDVLKEAVPG NIRRAEHFIA FLKRFVEYLK TRMKVLHVIA ETPTSFLQHV
370 380 390 400 410 420
KDITFIDKKP LRFCAERLTS LVRALQISLV EDFHSLQQVV AFATLVATYE RGFILILEPF
430 440 450 460 470 480
ETENATVPNP ILRFSCLDAS IAIKPVFERF RSVIITSGTL SPLDMYPKML QFNTVMQESY
490 500 510 520 530 540
GMSLARNCFL PMVVTRGSDQ VAISSKFEAR NDPSVVRNYG NILVEFSKIT PDGLVAFFPS
550 560 570 580 590 600
YLYLESIVSS WQSMGILDEV WKYKLILVET PDPHETTLAL ETYRAACSNG RGAVLLSVAR
610 620 630 640 650 660
GKVSEGVDFD HHYGRAVIMF GIPYQYTESR VLKARLEFLR DTYQIREADF LTFDAMRHAA
670 680 690 700 710 720
QCLGRVLRGK DDHGIMVLAD KRYGRSDKRT KLPKWIQQYI TEGATNLSTD MSLALAKKFL
730 740 750 760 770
RTMAQPFTAS DQEGISWWSL DDLLIHQKKA LKSAAIEQSK HEDEMDIDVV ET