P16658

PR
Gene name
SEN2 (YLR105C, L8004.12)
Protein name
tRNA-splicing endonuclease subunit SEN2
Names
Splicing endonuclease protein 2, tRNA-intron endonuclease SEN2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YLR105C
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

64-249 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P16658

Entry ID Method Resolution Chain Position Source
AF-P16658-F1 Predicted AlphaFoldDB

7 variants for P16658

Variant ID(s) Position Change Description Diseaes Association Provenance
s12-347975 69 D>E No SGRP
s12-347856 109 H>P No SGRP
292 G>E the cold-sensitive allele sen2-3; defective in cleavage only at the 5'-splice site of tRNA precursors [UniProt] No
s12-347272 304 G>S No SGRP
s12-347157 342 Q>R No SGRP
s12-347146 346 A>S No SGRP
s12-347145 346 A>V No SGRP

No associated diseases with P16658

1 regional properties for P16658

Type Name Position InterPro Accession
domain tRNA intron endonuclease, catalytic domain-like 259 - 337 IPR006677

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
  • Endomembrane system ; Peripheral membrane protein
  • Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side
  • The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
endomembrane system A collection of membranous structures involved in transport within the cell. The main components of the endomembrane system are endoplasmic reticulum, Golgi bodies, vesicles, cell membrane and nuclear envelope. Members of the endomembrane system pass materials through each other or though the use of vesicles.
mitochondrial outer membrane The outer, i.e. cytoplasm-facing, lipid bilayer of the mitochondrial envelope.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
tRNA-intron endonuclease complex A protein complex that catalyzes the endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini, and specifically removing the intron.

3 GO annotations of molecular function

Name Definition
lyase activity Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but only one in the other direction. When acting on the single substrate, a molecule is eliminated and this generates either a new double bond or a new ring.
nucleic acid binding Binding to a nucleic acid.
tRNA-intron endonuclease activity Catalysis of the endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini, and specifically removing the intron.

2 GO annotations of biological process

Name Definition
mRNA cleavage involved in mRNA processing Cleavage of an immature mRNA transcript to produce one or more more mature mRNA transcripts, prior to translation into polypeptide.
tRNA-type intron splice site recognition and cleavage RNA processing that begins when the tertiary structure of a tRNA type intron is recognized, and ends when the endonucleolytic cleavage of the RNA at both the 5' and 3' splice sites occurs.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MSKGRVNQKR YKYPLPIHPV DDLPELILHN PLSWLYWAYR YYKSTNALND KVHVDFIGDT
70 80 90 100 110 120
TLHITVQDDK QMLYLWNNGF FGTGQFSRSE PTWKARTEAR LGLNDTPLHN RGGTKSNTET
130 140 150 160 170 180
EMTLEKVTQQ RRLQRLEFKK ERAKLERELL ELRKKGGHID EENILLEKQR ESLRKFKLKQ
190 200 210 220 230 240
TEDVGIVAQQ QDISESNLRD EDNNLLDENG DLLPLESLEL MPVEAMFLTF ALPVLDISPA
250 260 270 280 290 300
CLAGKLFQFD AKYKDIHSFV RSYVIYHHYR SHGWCVRSGI KFGCDYLLYK RGPPFQHAEF
310 320 330 340 350 360
CVMGLDHDVS KDYTWYSSIA RVVGGAKKTF VLCYVERLIS EQEAIALWKS NNFTKLFNSF
370
QVGEVLYKRW VPGRNRD